LOCUS       Exported                8815 bp ds-DNA     circular SYN 21-SEP-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 21, 2018 from SnapGene 4.2.4
            http://www.snapgene.com
COMMENT     Ligation of : 2642 pCDH1_SBP-EGFP-gp135_hygro.xdna [7883  nt ] : 
            (PacI[4741] / AscI[2297]) to 1740_pA_CS-KDEL_Il2ss-SBP-E GFP.gb [926
             nt] : (AscI[2525] / PacI[3454])
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2305..2358
                     /label=signal peptide
     CDS             2365..2475
                     /label=SBP tag
                     /note="note"
     CDS             2485..3198
                     /label=EGFP
                     /note="note"
     misc_feature    3255..3845
                     /label=IRES
                     /note="label=IRES - 1333..1923"
     misc_feature    3869..4900
                     /label=Hyg(R)
                     /note="label=Hyg(R) - 1947..2978"
     CDS             complement(5381..5392)
                     /label=FactorXa site
     primer_bind     complement(6186..6206)
                     /label=M13-rev
                     /note="note"
     misc_binding    complement(6212..6234)
                     /label=LacO
                     /note="note"
     promoter        complement(6239..6268)
                     /label=lac
                     /note="note"
     rep_origin      complement(6574..7202)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(7354..8013)
                     /label=AmpR
                     /note="note"
     promoter        complement(8253..8281)
                     /label=Amp prom
                     /note="note"
     CDS             complement(8650..8718)
                     /label=LacZ alpha
     primer_bind     8789..8806
                     /label=M13-fwd
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcaccggt ggatccggcg cgccatgtac aggatgcaac tcctgtcttg cattgcacta
     2341 agtcttgcac ttgtcacgaa ttccgacgag aagaccactg gttggcgagg tggacacgtt
     2401 gttgaaggac tggctgggga acttgaacaa cttcgtgcac gactggagca tcacccacaa
     2461 ggtcaacgtg aaccacctgc aggtgtgagc aagggcgagg agctgttcac cggggtggtg
     2521 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2581 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2641 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2701 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2761 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2821 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2881 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2941 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     3001 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     3061 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     3121 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3181 atggacgagc tgtacaaggg ccggccttaa ggcctcgagg gccaattaat taagcggccg
     3241 cactagagga attccgcccc tctccccccc ccccctctcc ctcccccccc cctaacgtta
     3301 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     3361 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     3421 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     3481 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     3541 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     3601 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     3661 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     3721 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     3781 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     3841 gataagcttg ccacaacccg taccaaagat ggatagatcc ggaaagcctg aactcaccgc
     3901 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     3961 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     4021 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     4081 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac
     4141 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     4201 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
     4261 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     4321 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     4381 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     4441 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     4501 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     4561 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     4621 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
     4681 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     4741 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     4801 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     4861 tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa taggtcgaca atcaacctct
     4921 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     4981 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     5041 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     5101 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     5161 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     5221 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     5281 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     5341 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     5401 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     5461 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga
     5521 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     5581 taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct ctctggttag
     5641 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     5701 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5761 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     5821 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     5881 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5941 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6001 atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6061 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6121 ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc
     6181 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6241 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6301 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6361 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6421 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6481 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6541 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6601 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6661 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6721 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6781 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6841 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6901 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6961 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7021 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7081 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7141 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7201 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7261 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7321 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7381 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7441 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7501 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7561 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7621 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7681 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7741 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7801 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7861 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7921 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7981 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8041 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8101 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8161 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8221 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8281 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8341 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     8401 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     8461 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     8521 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     8581 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     8641 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     8701 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     8761 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
