LOCUS       Exported                7389 bp DNA     circular SYN 24-JUL-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Yoav
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 7, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        17..396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        397..600
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             601..776
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    839..1038
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1239..1655
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     enhancer        1663..2010
                     /label=CMV-IE
     promoter        2013..2290
                     /label=chicken beta-actin promoter
     intron          2606..2699
                     /label=beta-globin intron
     CDS             2712..3149
                     /codon_start=1
                     /label=TVA
                     /translation="MARLLPALLLLLLPGNVTGNGSGNGFLSRCPPGQFRCSEPPGAHG
                     ECYPQDWLCDGHPDCDDGRDEWGCGTSAIPAVPTDNGTEAPTAPASGRALPARNHGRMW
                     MLITAGPLCCLVAVGGIAAWGKSKAKSRFDIFSLERASKGGG"
     misc_feature    3161..3748
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             3781..4380
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     LTR             4876..5345
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     rep_origin      complement(5646..6234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6394..7254)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      421 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      481 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      541 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      601 caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc
      661 cgggtacccg tattcccaat aaagcctctt gctgtttgca tccgaatcgt ggtctcgctg
      721 ttccttggga gggtctcctc tgagtgattg actacccacg acgggggtct ttcatttggg
      781 ggctcgtccg ggatttggag acccctgccc agggaccacc gacccaccac cgggaggtaa
      841 gctggccagc aacttatctg tgtctgtccg attgtctagt gtctatgttt gatgttatgc
      901 gcctgcgtct gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg
      961 acgagttctg aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt
     1021 tttgtggccc gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg
     1081 ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg
     1141 tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc
     1201 tctgtctgac tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct
     1261 taagtttgac cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag
     1321 atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg
     1381 gatggccgcg agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct
     1441 tttcacctgg cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct
     1501 tggcttttga cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc
     1561 ttcctccatc cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct
     1621 ccctttatcc agccctcact ccttctctag gcgccggaat tgcagagagg gagtggccaa
     1681 ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta
     1741 gccatgctct aggaagatcg taccattgac gtcaataatg acgtatgttc ccatagtaac
     1801 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     1861 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     1921 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     1981 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
     2041 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
     2101 ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg
     2161 cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
     2221 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     2281 gcggcgggcg ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc gccgccgcct
     2341 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
     2401 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt
     2461 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg
     2521 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2581 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2641 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg
     2701 gatccgccac catggcgcgg ctgctgcccg cgctgctgct gctgctgctg cccggtaacg
     2761 tgaccggtaa cgggtccggt aacggttttt tgtcccgttg cccccccggt cagttccgct
     2821 gctcggagcc gcccggtgcc cacggggagt gttacccgca ggactggctg tgcgacggac
     2881 accccgactg cgacgacggg cgggacgagt gggggtgcgg gaccagcgcg atccccgcgg
     2941 tgcccacgga caacggcaca gaggctccca ctgcccctgc ttctggacgt gctttgccag
     3001 ccaggaatca cggccgcatg tggatgctga tcactgcagg gcccctgtgc tgcctggtag
     3061 ctgtgggggg tatcgctgca tgggggaagt ccaaagcaaa aagcaggttt gacattttca
     3121 gtcttgaaag ggcatccaag gggggtgggt gagaattccg cccccccccc ccccccccct
     3181 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     3241 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     3301 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     3361 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     3421 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     3481 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     3541 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     3601 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     3661 gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt
     3721 ggttttcctt tgaaaaacac gatgataagc ttgccacaac ccacaaggag acgaccttcc
     3781 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta
     3841 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac
     3901 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     3961 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     4021 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     4081 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     4141 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     4201 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     4261 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     4321 gacgtcgagt gcccgaagga ccgcgcgacc tggtgcatga cccgcaagcc cggtgcctga
     4381 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tggctccgac
     4441 cgaagccgac ccgggcggcc ccgccgaccc cgcacccgcc cccgaggccc accgactcta
     4501 gtcgagggct gcagcgctgc agaggccgag tgcagaactg ctccaaaggg acctcaaggc
     4561 tttccgaggg acactaggct gactccatcg agccagtgta gagataagct tatcgattag
     4621 tccaatttgt taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc
     4681 accagctgaa gcctatagag tacgagccat agataaaata aaagatttta tttagtctcc
     4741 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     4801 cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag
     4861 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     4921 ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
     4981 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc
     5041 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
     5101 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
     5161 ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg
     5221 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
     5281 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
     5341 tttcacatgc agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa
     5401 tggccatagt tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5461 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5521 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5581 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5641 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5701 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5761 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5821 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5881 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     5941 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6001 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6061 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6121 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6181 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6241 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6301 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgcggcc gcaaatcaat
     6361 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6421 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6481 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6541 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6601 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6661 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6721 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6781 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6841 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6901 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6961 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7021 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7081 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7141 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7201 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7261 cctttttcaa tgatccggcc attagccata ttattcattg gttatatagc ataaatcaat
     7321 attggctatt ggccattgca tacgttgtat ccatatcata atatgtacat ttatattggc
     7381 tcatgtcca
//
