LOCUS       Exported                8529 bp DNA     circular SYN 22-MAR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Yoav
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 7, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        17..396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        397..600
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             601..776
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    839..1038
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1239..1655
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     enhancer        1663..2010
                     /label=CMV-IE
     promoter        2013..2290
                     /label=chicken beta-actin promoter
     intron          2606..2699
                     /label=beta-globin intron
     CDS             2718..4292
                     /codon_start=1
                     /label=B19-G
                     /translation="MVPQVLLFVLLLGFSLCFGKFPIYTIPDKLGPWSPIDIHHLRCPN
                     NLVVEDEGCTNLSGFSYMELKVGYISAIKVNGFTCTGVVTEAETYTNFVGYVTTTFKRK
                     HFRPTPDACRAAYNWKMAGDPRYEESLQNPYPDYHWLRTVRTTKESLIIISPSVTDLDP
                     YDKSLHSRVFPGGKCSGITVSSTYCSTNHDYTIWMPEDPRPGTPCDIFTNSRGKRASNG
                     NKTCGFVDERGLYKSLKGACRLKLCGVLGLRLMDGTWVAMQTSDETKWCSPDQLVNLHD
                     FRSNEIEHLVVEDLVKKREECLDTLESIMTTKSVSFRRLSHLRKLVPGFGKAYTIFNKT
                     LMEADVHYKSVRTWNEIIPSKGCLKVGGRCHPHVNGVFFNGIILGPDDRVLIPEMQSSL
                     LRQHMELLESSVIPLMHPLADPSTVFKEGDEAEDFVEVHLPDVYKKISGVDLGLPNWGK
                     YVLMTAGAMIGLVLIFSLMTWCRRANRPESKQRSFGGTGGNVSVTSQSGKVIPSWESYK
                     SGGEIRL"
     misc_feature    4301..4888
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             4921..5520
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     LTR             6016..6485
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     rep_origin      complement(6786..7374)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7534..8394)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      421 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      481 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      541 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      601 caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc
      661 cgggtacccg tattcccaat aaagcctctt gctgtttgca tccgaatcgt ggtctcgctg
      721 ttccttggga gggtctcctc tgagtgattg actacccacg acgggggtct ttcatttggg
      781 ggctcgtccg ggatttggag acccctgccc agggaccacc gacccaccac cgggaggtaa
      841 gctggccagc aacttatctg tgtctgtccg attgtctagt gtctatgttt gatgttatgc
      901 gcctgcgtct gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg
      961 acgagttctg aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt
     1021 tttgtggccc gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg
     1081 ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg
     1141 tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc
     1201 tctgtctgac tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct
     1261 taagtttgac cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag
     1321 atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg
     1381 gatggccgcg agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct
     1441 tttcacctgg cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct
     1501 tggcttttga cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc
     1561 ttcctccatc cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct
     1621 ccctttatcc agccctcact ccttctctag gcgccggaat tgcagagagg gagtggccaa
     1681 ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta
     1741 gccatgctct aggaagatcg taccattgac gtcaataatg acgtatgttc ccatagtaac
     1801 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     1861 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     1921 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     1981 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
     2041 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
     2101 ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg
     2161 cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
     2221 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     2281 gcggcgggcg ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc gccgccgcct
     2341 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
     2401 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt
     2461 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg
     2521 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2581 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2641 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg
     2701 gatcctctag agccaccatg gttcctcagg ttcttttgtt tgtactcctt ctggggtttt
     2761 cgttgtgttt cgggaagttc cccatttaca cgataccaga caaacttggt ccctggagcc
     2821 ctattgacat acaccatctc cgctgtccaa ataacctggt tgtggaggat gaaggatgta
     2881 ccaacctgtc cgggttctcc tatatggaac tcaaagtggg atacatctca gccatcaaag
     2941 tgaacgggtt cacttgcaca ggtgttgtga cagaggcaga gacctacacc aactttgttg
     3001 gttatgtcac aaccacattc aagagaaagc atttccgccc caccccagac gcatgtagag
     3061 ccgcgtataa ctggaagatg gccggtgacc ccagatatga agagtcccta caaaatccat
     3121 accccgacta ccactggctt cgaactgtaa gaaccaccaa agagtccctc attatcatat
     3181 ccccaagtgt gacagatttg gacccatatg acaaatccct tcactcaagg gtcttccctg
     3241 gcggaaagtg ctcaggaata acggtgtcct ctacctactg ctcaactaac catgattaca
     3301 ccatttggat gcccgaggac ccgagaccag ggacaccttg tgacattttt accaatagca
     3361 gagggaagag agcatccaac gggaacaaga cttgcggctt tgtggatgaa agaggcctgt
     3421 ataagtctct aaaaggagca tgcaggctca agttatgtgg agttcttgga cttagactta
     3481 tggatggaac atgggtcgcg atgcaaacat cagatgagac caaatggtgc tctccagatc
     3541 agttggtgaa tttgcacgac tttcgctcaa acgagattga gcatctcgtt gtggaggatt
     3601 tagtcaagaa aagagaggaa tgtctggata cattagagtc catcatgacc accaagtcag
     3661 taagtttcag acgtctcagt cacctgagaa aacttgtccc agggtttgga aaagcatata
     3721 ccatattcaa caaaaccttg atggaggctg atgttcacta caagtcagtc cggacctgga
     3781 atgagatcat cccctcaaaa gggtgtttga aagttggagg aaggtgccat cctcatgtga
     3841 acggggtgtt tttcaatggt ataatattag ggcctgacga ccgtgtccta atcccagaga
     3901 tgcaatcatc cctcctccgg caacatatgg agttgttgga atcttcagtt atccccctga
     3961 tgcaccccct ggctgaccct tctacagttt tcaaagaagg tgatgaggct gaggattttg
     4021 ttgaagttca cctccccgat gtgtacaaaa agatctcagg ggttgacctg ggtctcccga
     4081 actggggaaa gtatgtattg atgactgcag gggccatgat tggcctggtg ttgatatttt
     4141 ccctaatgac atggtgcaga agagccaatc gaccagaatc gaaacaacgc agttttggag
     4201 ggacaggggg gaatgtgtca gtcacttccc aaagcggaaa agtcatacct tcatgggaat
     4261 catataagag tggaggtgag atcagactgt aagaattccg cccccccccc ccccccccct
     4321 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     4381 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     4441 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     4501 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     4561 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     4621 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     4681 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     4741 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     4801 gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt
     4861 ggttttcctt tgaaaaacac gatgataagc ttgccacaac ccacaaggag acgaccttcc
     4921 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta
     4981 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac
     5041 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     5101 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     5161 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     5221 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     5281 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     5341 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     5401 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     5461 gacgtcgagt gcccgaagga ccgcgcgacc tggtgcatga cccgcaagcc cggtgcctga
     5521 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tggctccgac
     5581 cgaagccgac ccgggcggcc ccgccgaccc cgcacccgcc cccgaggccc accgactcta
     5641 gtcgagggct gcagcgctgc agaggccgag tgcagaactg ctccaaaggg acctcaaggc
     5701 tttccgaggg acactaggct gactccatcg agccagtgta gagataagct tatcgattag
     5761 tccaatttgt taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc
     5821 accagctgaa gcctatagag tacgagccat agataaaata aaagatttta tttagtctcc
     5881 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     5941 cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag
     6001 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     6061 ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
     6121 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc
     6181 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
     6241 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
     6301 ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg
     6361 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
     6421 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
     6481 tttcacatgc agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa
     6541 tggccatagt tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     6601 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     6661 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6721 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6781 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6841 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6901 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6961 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7021 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7081 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7141 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7201 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7261 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7321 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7381 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7441 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgcggcc gcaaatcaat
     7501 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7561 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7621 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7681 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7741 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7801 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7861 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7921 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7981 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8041 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8101 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8161 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8221 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8281 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8341 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8401 cctttttcaa tgatccggcc attagccata ttattcattg gttatatagc ataaatcaat
     8461 attggctatt ggccattgca tacgttgtat ccatatcata atatgtacat ttatattggc
     8521 tcatgtcca
//
