LOCUS       Exported                9288 bp DNA     circular SYN 05-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Yoav Ben Simon
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 7, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        17..396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        397..600
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             601..776
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    839..1038
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1239..1655
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     enhancer        1663..2010
                     /label=CMV-IE
     promoter        2013..2290
                     /label=chicken beta-actin promoter
     intron          2606..2699
                     /label=beta-globin intron
     CDS             2713..5364
                     /codon_start=1
                     /label=oT7-Pol
                     /translation="MNTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYE
                     MGEARFRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTAFQ
                     FLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFK
                     KNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIE
                     STGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRAGALAGISPMFQPCVVPPKPWTGI
                     TGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANV
                     ITKWKHCPVEDIPAIEREELPMKPEDIDMNPEALTAWKRAAAAVYRKDKARKSRRISLE
                     FMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYY
                     WLKIHGANCAGVDKVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPFCFLAFCFE
                     YAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAK
                     KVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALAGQWLAYGVTRSVTKRSVM
                     TLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVE
                     AMNWLKSAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGFPVWQEYKKPIQTRLNLMFLG
                     QFRLQPTINTNKDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHD
                     SFGTIPADAANLFKAVRETMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNLN
                     LRDILESDFAFA"
     misc_feature    5376..5948
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6007..6405
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6775..7244
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     rep_origin      complement(7545..8133)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8293..9153)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      421 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      481 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      541 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      601 caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc
      661 cgggtacccg tattcccaat aaagcctctt gctgtttgca tccgaatcgt ggtctcgctg
      721 ttccttggga gggtctcctc tgagtgattg actacccacg acgggggtct ttcatttggg
      781 ggctcgtccg ggatttggag acccctgccc agggaccacc gacccaccac cgggaggtaa
      841 gctggccagc aacttatctg tgtctgtccg attgtctagt gtctatgttt gatgttatgc
      901 gcctgcgtct gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg
      961 acgagttctg aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt
     1021 tttgtggccc gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg
     1081 ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg
     1141 tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc
     1201 tctgtctgac tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct
     1261 taagtttgac cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag
     1321 atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg
     1381 gatggccgcg agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct
     1441 tttcacctgg cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct
     1501 tggcttttga cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc
     1561 ttcctccatc cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct
     1621 ccctttatcc agccctcact ccttctctag gcgccggaat tgcagagagg gagtggccaa
     1681 ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta
     1741 gccatgctct aggaagatcg taccattgac gtcaataatg acgtatgttc ccatagtaac
     1801 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     1861 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     1921 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     1981 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
     2041 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
     2101 ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg
     2161 cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
     2221 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     2281 gcggcgggcg ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc gccgccgcct
     2341 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
     2401 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt
     2461 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg
     2521 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2581 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2641 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg
     2701 gatcccgcca ccatgaacac catcaatatt gccaagaacg acttttctga tatcgagctg
     2761 gccgctattc cattcaatac actggctgac cactacggag agcggctggc ccgcgaacag
     2821 ctggctctgg agcatgaaag ctatgagatg ggagaagccc gattcaggaa gatgtttgag
     2881 aggcagctga aagctgggga agtggcagac aacgcagccg ctaagccact gattaccaca
     2941 ctgctgccca aaatgatcgc cagaattaat gattggttcg aggaagtgaa ggcaaaacga
     3001 ggaaagaggc ctaccgcctt ccagtttctg caggagatca agccagaagc tgtggcatac
     3061 atcaccatca agactaccct ggcatgcctg acaagcgccg acaacacaac tgtgcaggca
     3121 gtcgcttccg caatcggacg agctattgag gacgaagcac gctttgggag aatccgggat
     3181 ctggaggcca agcacttcaa gaaaaacgtg gaggaacagc tgaacaagag ggtggggcat
     3241 gtctataaga aggccttcat gcaggtggtc gaggccgaca tgctgtcaaa gggactgctg
     3301 ggaggagagg cctggagctc ctggcacaaa gaagatagca tccatgtggg agtccgctgc
     3361 atcgagatgc tgattgaatc tactgggatg gtgagtctgc accgacagaa cgccggcgtg
     3421 gtcggacagg actctgagac aatcgaactg gctcccgagt atgccgaagc tattgcaact
     3481 agagccggag ctctggcagg gatcagtcca atgttccagc cctgcgtggt cccccctaag
     3541 ccttggactg gcatcaccgg gggcggatac tgggctaatg gaaggagacc actggcactg
     3601 gtgcgaacac actctaagaa agccctgatg agatacgagg atgtctatat gcccgaagtg
     3661 tataaggcca tcaacattgc tcagaataca gcatggaaaa ttaacaagaa agtgctggcc
     3721 gtcgctaatg tgatcactaa gtggaaacat tgtcccgtgg aggacatccc tgccattgaa
     3781 cgggaggaac tgcctatgaa gccagaggac atcgatatga accctgaagc tctgaccgca
     3841 tggaagcgcg cagccgctgc agtgtacaga aaggataaag cccggaagtc ccggcgcatt
     3901 tctctggagt tcatgctgga acaggccaac aagtttgcta atcacaaagc aatctggttc
     3961 ccctacaaca tggactggcg cggacgagtc tatgccgtga gcatgttcaa ccctcagggg
     4021 aatgatatga caaagggcct gctgactctg gctaagggga aaccaattgg gaaggagggc
     4081 tactattggc tgaaaatcca cggggccaat tgcgctggcg tcgacaaggt gccattcccc
     4141 gagaggatca agttcatcga ggaaaaccat gaaaatatta tggcatgtgc caagtctccc
     4201 ctggagaaca catggtgggc cgaacaggat agtcctttct gctttctggc cttctgtttt
     4261 gagtacgctg gagtgcagca ccatgggctg agttataatt gctccctgcc actggccttt
     4321 gacggctctt gtagtggaat ccagcacttc tccgcaatgc tgagggatga ggtcggagga
     4381 agagcagtga acctgctgcc atctgagaca gtgcaggaca tctacggcat tgtcgccaag
     4441 aaagtgaatg agatcctgca ggctgacgca attaacggga ctgataatga ggtggtcacc
     4501 gtcacagatg aaaacactgg cgagatcagc gaaaaggtga aactgggaac caaggccctg
     4561 gctggacagt ggctggcata cggagtcacc cgctcagtga caaagcgaag cgtgatgacc
     4621 ctggcttatg gcagcaaaga gttcggcttc aggcagcagg tgctggaaga caccatccag
     4681 ccagccattg attccggaaa ggggctgatg tttacacagc ccaaccaggc cgctggctac
     4741 atggccaagc tgatctggga gtcagtgagc gtcacagtgg tcgcagccgt ggaagctatg
     4801 aattggctga agtccgctgc aaaactgctg gccgctgagg tgaaggacaa gaaaactggc
     4861 gaaattctga ggaaaagatg cgccgtccac tgggtgaccc ctgatggatt cccagtgtgg
     4921 caggagtata agaaacccat ccagaccaga ctgaacctga tgttcctggg ccagtttcgg
     4981 ctgcagccta caatcaacac taataaggac agtgagattg atgctcataa acaggaatca
     5041 gggattgcac ctaattttgt gcacagccag gacggctccc atctgcggaa gactgtggtc
     5101 tgggctcacg agaaatacgg catcgaatcc ttcgcactga ttcatgactc ttttggaacc
     5161 atcccagccg atgcagccaa cctgttcaag gctgtccgcg agactatggt ggacacctac
     5221 gaaagttgtg atgtgctggc cgacttctat gatcagtttg ctgaccagct gcacgagtca
     5281 cagctggata agatgcccgc actgcctgcc aaaggcaacc tgaatctgag agacatcctg
     5341 gagtccgatt tcgcatttgc ctgactcgaa ttccgcccct ctccctcccc cccccctaac
     5401 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     5461 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     5521 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     5581 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     5641 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     5701 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     5761 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     5821 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     5881 aggttaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     5941 gatgataagc ttgccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc
     6001 acaaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct
     6061 acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc
     6121 gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa
     6181 ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg
     6241 atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc
     6301 gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt
     6361 gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaagcact tcgtggccga
     6421 ggagcaggac tgacacgacc cggacgggac gctcctgcgc ctgatacaga acgaattgct
     6481 tgcaggcatc tcatgagtgt gtcttcccgt tttccgcctg aggtcactgc gtggatggag
     6541 catcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt
     6601 acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     6661 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
     6721 atacataact gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat
     6781 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     6841 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     6901 cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc
     6961 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
     7021 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
     7081 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg
     7141 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     7201 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcacatgca gcatgtatca
     7261 aaattaattt ggtttttttt cttaagtatt tacattaaat ggccatagtt gcattaatga
     7321 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7381 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7441 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7501 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7561 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7621 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7681 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7741 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7801 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7861 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7921 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7981 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8041 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8101 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8161 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8221 aggatcttca cctagatcct tttgcggccg caaatcaatc taaagtatat atgagtaaac
     8281 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8341 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8401 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     8461 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8521 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8581 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8641 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8701 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8761 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8821 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8881 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8941 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9001 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9061 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9121 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat gatccggcca
     9181 ttagccatat tattcattgg ttatatagca taaatcaata ttggctattg gccattgcat
     9241 acgttgtatc catatcataa tatgtacatt tatattggct catgtcca
//
