LOCUS       Exported               16605 bp DNA     circular SYN 16-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 7, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16605
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             3325..4755
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             4798..5859
                     /codon_start=1
                     /label=ChIEF
                     /translation="MSRRPWLLALALAVALAAGSAGASTGSDATVPVATQDGPDYVFHR
                     AHERMLFQTSYTLENNGSVICIPNNGQCFCLAWLKSNGTNAEKLAANILQWITFALSAL
                     CLMFYGYQTWKSTCGWEEIYVATIEMIKFIIEYFHEFDEPAVIYSSNGNKTVWLRYAAW
                     LLTCPVVLIHLSNLTGLANDYNKRTMGLLVSDIGCIVWGTTAALSKGYVRVIFFLMGLC
                     YGIYTFFNAAKVYIEAYHTVPKGRCRQVVTGMAWLFFVSWGMFPILFILGPEGFGVLSV
                     YGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDIRKTTKLNIGGTEIEVETLVE
                     DEAEAGAVVNGSG"
     CDS             8034..8045
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(9837..9848)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      13059..13106
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(13134..13150)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    13158..13174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(13182..13212)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13227..13248
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(13536..14124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(14295..15155)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(15156..15260)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      15287..15742
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     15883..15899
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15969..16272
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        16273..16476
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        16521..16539
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatggtg agcaagggcg aggaggtcat caaagagttc
     3361 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     3421 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     3481 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     3541 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     3601 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     3661 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     3721 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     3781 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     3841 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     3901 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     3961 gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg caccggcagc
     4021 accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc cgtcatcaaa
     4081 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc
     4141 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     4201 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc
     4261 aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag
     4321 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag
     4381 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc
     4441 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc
     4501 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac
     4561 ggcggccgct acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg
     4621 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc
     4681 atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta cggcatggac
     4741 gagctgtaca agtaagctag acatgaaaaa aactaacact cctccggtac cgccaccatg
     4801 tcgcggaggc catggcttct tgccctagcg ctggcagtgg cgctggcggc cggcagcgca
     4861 ggagcctcga ctggcagtga cgcgacggtg ccggtcgcga ctcaggatgg ccccgactac
     4921 gttttccacc gtgcccacga gcgcatgctc ttccaaacct catacactct tgagaacaat
     4981 ggttctgtta tttgcatccc gaacaacggc cagtgcttct gcttggcttg gcttaaatcc
     5041 aacggaacaa atgccgagaa gttggctgcc aacattctgc agtggattac ttttgcgctt
     5101 tcagcgctct gcctgatgtt ctacggctac cagacctgga agtctacttg cggctgggag
     5161 gagatttacg tggccacgat cgagatgatc aagttcatca tcgagtattt ccatgagttt
     5221 gacgaacctg cggtgatcta ctcatccaac ggcaacaaga ccgtgtggct tcgttacgcg
     5281 gcctggctgc tgacctgccc tgtcgttctt atccatctga gcaaccttac gggtctggcg
     5341 aacgactata acaagcgtac catgggtctg ctggtgtcag atatcggctg tatcgtgtgg
     5401 ggcaccacgg ccgcgctgtc caagggatac gtccgtgtca ttttcttcct gatgggcctg
     5461 tgctacggca tctacacatt cttcaacgca gccaaggtct acattgaggc gtaccacacc
     5521 gtgccgaagg gccggtgtcg ccaggtggtg actggcatgg cttggctctt cttcgtatca
     5581 tggggtatgt tccccatcct gttcatcctc ggccccgagg gcttcggcgt cctgagcgtg
     5641 tacggctcca ccgtcggcca caccatcatt gacctgatgt cgaagaactg ctggggtctg
     5701 ctcggccact acctgcgcgt gctgatccac gagcatatcc tcatccacgg cgacattcgc
     5761 aagaccacca aattgaacat tggtggcact gagattgagg tcgagacgct ggtggaggac
     5821 gaggccgagg ctggcgcggt cgttaacgga agcggataag ctagcggcgc ctagccggtc
     5881 atccttttga cgattccagt cccgaggata acctcctctc gggattgggg ggaatctttg
     5941 gatccagtag tcctccttga actccgtcca acagggcaga ttcaagagtc ataagacttt
     6001 cattaatcat ttcagttgat cagacatggt cgtgtagatt ctcttaatac gggagatctt
     6061 ctagcagttt cagtgaccaa cggtgctttc attctccagg aactgatacc aaaggttgtg
     6121 gacaggccaa ggggtacttc ggatgactct gtgcttgggc acagaaagag gtcgtagtgt
     6181 gccccccgat agcggactcg acatgaatca actaagaaag gcaatctgcc tcccatgatg
     6241 gacataagca atagttcaca accatcttgc atctcagtga agtgtacata actattgagg
     6301 gctgggtcat ctaagcattt cagtcgagaa aaaaactgta gaccaaaaga acaactagca
     6361 acacttctca tccagagacc catatcaaga tgctagatcc gggagaggtt tatgatgacc
     6421 ctattgatcc aattgagtca gaggctgaac ccagaggaac ccccactgtc cccaacatct
     6481 tgaggaactc cgactacaat ctcaattctc ctttgataga ggatcctgcc aaactaatgt
     6541 tagaatggtt gaagacaggg aacagacctt atcggatgac tttgacagac aattgctcca
     6601 ggtcttacaa agttttgaaa gattatttca agaaagtaga tttgggttct ctcaaagtgg
     6661 gcggaactgc tgcacagtca atggtttctc tctggttgtg tggtgcccac tctgaatcaa
     6721 acaggagccg gagatgtata accgacttgg cccatttcta ttccaagtca tcccccatag
     6781 agaagctatt gaattgtacg ctaggaaaca gaggcctgag aatcccacca gagggggtgt
     6841 taaattgcct cgagagggtc aattatgaca aggcatttgg gaggtatctg gccaacacgt
     6901 attcctctta cttgtttttc catgtaatca ccttatacat gaatgcctta gactgggaag
     6961 aggaaaagac catcctggca ttatggaaag atataacctc agtggatacc gagaaggact
     7021 tggtcaaatt caaagatcaa atatggggac tgttgattgt gacaaaggac tttgtttact
     7081 ctcagagttc aaactgtctt tttgacagaa actacacact gatgctaaag gatcttttct
     7141 tgtctcgatt caactcctta atgattctgc tttctccccc tgagccccga tactcagatg
     7201 acttaatatc tcagctgtgc cagctataca tcgctgggga tcaagtcttg tccatgtgtg
     7261 ggaactccgg ctatgaagtc atcaaaatat tggagccata tgtcgtgaac agtttggtcc
     7321 agagggcaga gaagtttagg cctctcatcc accccttggg agactttcct atgttcataa
     7381 aagacaaggt gaatcaactt gaagggactt ttggtcccag tgcaaagagg ttttttaggg
     7441 ttttagatca attcgacaac atacatgact tagtatttgt gtatggctgt tacagacatt
     7501 gggggcaccc ctatatagat tatcggaagg gtctgtcgaa actatatgat caagttcaca
     7561 ttaagaaagt aatagataag tcctaccagg agtgtttagc aagtgacttg gccagaagga
     7621 tcctcagatg gggatttgac aagtactcca aatggtatct agattcgaga ttccttgccc
     7681 gagaccaccc cttgactcct tatgtcaaga cccaaacatg gccacccaaa catatagtag
     7741 acttggtggg ggacacatgg cataagctcc cgatcacgca gatctttgaa attcctgaat
     7801 caatggaccc gtcagagata ctggatgaca aatcacattc tttcaccaga acaaggttag
     7861 cttcttggct gtcagagaac cgaggggggc ctgttcctag cgagaaggtc attatcacgg
     7921 ccctgtctaa gccacctgtc aatccccgag agtttttgaa atctatagac ctcggaggat
     7981 tgccagatga ggatttgata attggcctca aaccaaagga acgggagttg aagatcgagg
     8041 gccgattctt tgctctaatg tcatggaatc taagattata ttttgtcatc accgaaaagc
     8101 tcttggccaa ctacattttg ccactttttg acgcactgac tatgacagac aacctgaaca
     8161 aggtgttcaa aaagttgatc gacagggtca ccgggcaagg gcttttggac tattctaggg
     8221 tcacatacgc atttcacctg gactatgaga aatggaacaa tcatcaaaga ttggagtcaa
     8281 cagaggatgt attctctgtc ctagatcagg tgtttggatt gaagagggtg ttttctagaa
     8341 cacacgagtt ttttcagaag tcctggatct attattcaga cagatcagac ctcattgggt
     8401 tatgggagga ccaaatatat tgcttggata tgtctaacgg cccaacctgc tggaatggcc
     8461 aagatggcgg gctagagggc ttgcggcaga agggctggag tctagtcagt ttattaatga
     8521 tagatagaga atctcaaacc aggaacacaa gaaccaagat actagctcaa ggagacaacc
     8581 aggttctgtg tcctacatac atgttgtcac cgggattgtc tcaagagggg cttctctatg
     8641 agttagagag catatcaagg aatgcactct caatataccg agctatcgag gaaggagcat
     8701 ctaagctggg gctgatcatc aagaaggaag agaccatgtg tagttatgac tttctcatat
     8761 atgggaagac ccccttattt cgaggcaaca tattggtgcc tgaatccaaa agatgggccc
     8821 gagtctcttg catctctaac gaccaaatag tcaacctcgc caatataatg tcgacagtat
     8881 ccaccaatgc gctgacagtg gcacaacact ctcaatctct gatcaaacct atgagggatt
     8941 ttctgctcat gtcagtacag gcagttttcc actacctgtt gtttagccca atcctaaaag
     9001 gcagagttta taagattctg agtgctgaag gggagagctt tctcctagcc atgtcgcgga
     9061 taatctacct agatccttct ttgggagggg tgtctggaat gtctctcggg aggttccata
     9121 tacgtcagtt ctcagaccct gtctctgaag ggttgtcatt ctggagagag atctggttag
     9181 gctctcatga gtcctggatt cacgcgttgt gtcaggaggc cgggaacccc gatcttggag
     9241 agagaacact cgagagcttc actcgccttt tagaagatcc tactacctta aatatcaaag
     9301 gaggggccag ccctaccatt ctactcaagg atgctatcag aaaggctctg tacgacgagg
     9361 tggacaaggt ggaaaattca gagtttcgag aggcaatcct gctgtccaag acccatagag
     9421 ataactttat actcttttta aaatctgttg agcctctgtt ccctcgattt ctcagtgagc
     9481 tcttcagttc gtctttcttg ggaataccgg agtcaatcat tggactgata caaaactccc
     9541 ggacaataag aaggcagttt agaaagagtc tctcaagaac tttagaagag tccttctaca
     9601 actcagagat ccacgggatt aatcggataa cccagacacc tcaaagggtc ggaagggtgt
     9661 ggccttgctc ttcagagagg gcggatctac ttagggagat ctcttggggg aggaaagtgg
     9721 taggcacgac agttcctcac ccttccgaga tgttggagtt gtttcccaaa tcctccattt
     9781 cctgcacttg tggagcaaca gggggaggca atcctagagt ctctgtatca gtactcccgt
     9841 ccttcgatca gtcatttttc tcacggggcc ccctaaaggg atacttgggc tcgtccacct
     9901 ctatgtcaac ccagctattc catgcatggg aaaaagtcac taatgttcat gtggtgaaaa
     9961 gggctctatc gttaaaagaa tctataaact ggttcatcaa taggaattcc aatttggctc
    10021 aaactctaat tggaaacatc atgtctctga caggccctga cttccctcta gaagaggccc
    10081 ctgttttcaa acggacaggg tcagccttgc ataggttcaa gtctgccaga tacagcgaag
    10141 gagggtattc ttctgtttgc cctaaccttc tctcccatat ctctgttagt acagacacta
    10201 tgtctgattt gacccaaaac gggaagaact atgatttcat gtttcagcca ttgatgcttt
    10261 atgcgcaaac atggacatcg gaactggtac agagggatac aagacttaga gactccacgt
    10321 ttcactggca ccttcggtgc aacagatgtg tgaggcccat tgatgatata acactggaaa
    10381 cttctcagat cttcgagttc ccggatgtgt caaaaaggat atccaggatg gtttctggag
    10441 ctgtccctca atttcagaag cttcctgata tccgtctaag accaggtgat tttgaatccc
    10501 taagtggtag agaaaagtct cgccatatag ggtcagctca ggggctctta tactcaatct
    10561 tagtagcaat tcacgactca ggatacaatg atgggaccat cttccctgtc aacatatacg
    10621 gcaaagtttc ccccagagac tatttgagag ggcttgcaag agggatcttg atagggtcct
    10681 cgatttgctt cttgacacga atgacaaata ttaacattaa cagacctctt gaattgatct
    10741 caggggtaat ttcctatatt ctcctgaggc tggataatca tccctctctg tatataatgc
    10801 tcagagaacc gtctcttaga ggagaaatat tctctatccc tcagaaaatc cccgccgctt
    10861 acccaaccac tatgagagaa ggcaacagat cgattttgtg ttacctccaa cacgtgctac
    10921 gctatgagcg agaggcaatc acggcgtccc cggagaatga ctggctgtgg atcttctcag
    10981 acttcagaag tgtgaaaatg acgtacttga ccctcattac ttaccagtct cacctcctac
    11041 tccagagggt tgagagaaac ttgtctaaga gtatgagagc tactctgcga caaatgggtt
    11101 ccttaatgag gcaagtgctg ggtgggcacg gagaagatac cttggagtca gacgatgaca
    11161 ttcaacgatt actaaaagac tctttgcgaa agacaaggtg ggtggatcaa gaggtgcgcc
    11221 atgcagctag aaccatgaat ggagattaca gccccgacaa gaaagtatcc cgtaaggtag
    11281 gatgttcaga atgggtctgc tctgctcaac agattgccgt ctccacctca gccaacccgg
    11341 cccctgtctc agagcttgac attagggccc tctctaagcg gtttcaaaac cccttgatct
    11401 cgggcctgag agtggttcag tgggcaaccg gtgcccatta taagcttaag cctattctag
    11461 atgatctaaa tgttttccca tctctctgtc ttgtaattgg agacgggtca gggggaatat
    11521 caagggcagt tctcaacatg tttccagatt ctaagcttgt gttcaacagc ctattggagg
    11581 tgaatgatct gatggcttcc ggaacacatc cactgcctcc ttcagcaatc atgagtggag
    11641 gagatgacat catctccaga gtgatagact ttgactcaat atgggagaaa ccgtccgacc
    11701 tgaggaactc ggccacctgg agatacttcc agtcggttca aaaacaggtc aacatgtcgt
    11761 atgacctcat tatttgtgat gcagaagtta ctgatattgc atctatcaac cggataactc
    11821 tgttgatgtc tgatttcgca ttgtctatag atggaccact ttatctggtc ttcaaaactt
    11881 acgggactat gctagtaaac ccggactata aagctattca acatctgtca agagcgttcc
    11941 cttcggtcac agggtttgta acccaagtaa cttcatcctt ttcttctgag ctatacctcc
    12001 ggttctctaa acgaggaaag tttttcaggg acgccgagta cttgacctct tccacccttc
    12061 gagagatgag ccttgtgttg ttcaattgca gcagccccaa aagtgagatg cagagagctc
    12121 gttccttaaa ctatcaagac ctggtaaggg gatttcctga agagatcata tcaaatcctt
    12181 acaacgaaat gatcataact ctgattgaca atgatgtaga gtccttccta gtccacaaga
    12241 tggtggatga tcttgagcta cagaggggaa ctctgtctaa agtggctatc attatatcca
    12301 tcatgatcgt tttttccaat agagtcttca acatttccaa acctttgact gaccccttgt
    12361 tctatccacc gtctgatcct aaaatcctga ggcacttcaa catatgttgc agtactatga
    12421 tgtatctatc taccgcttta ggcgacgtcc ctaacttcgc aagacttcat gacctgtata
    12481 acagacctat aacttgttac ttcagaaaac aagttattcg agggaatatt tatctatctt
    12541 ggagttggtc cgatgatacc ccagtgttca agagagtagc ctgtaattct agcttgagtc
    12601 tgtcatctca ctggatcagg ttgatctaca agatagtgaa gactaccaga ctcattggca
    12661 gcataaaaga cctatcagga gaggtagaac gacatcttca tgggtataac agatggatca
    12721 ccctcgagga tatccgatct agatcatccc tactagatta cagttgtttg taagccggat
    12781 attaccgaaa gcctgtgcat gctaaaattc ttgtatgatg catcttgaaa aaaacaagat
    12841 cttgaatccg gacctctggt tgtttgattg ttttttccat ctttattgtt tttttgttaa
    12901 gcgtgggtcg gcatggcatc tccacctcct cgcggtccga cctgggcatc cgaaggagga
    12961 cgcacgtcca ctcggatggc taagggaggg gcggggatcc ggctgctaac aaagcccgaa
    13021 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
    13081 ctaaacgggt cttgaggggt tttttgctga aagtcgcgct tggcgtaatc atggtcatag
    13141 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    13201 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    13261 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
    13321 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
    13381 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    13441 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    13501 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    13561 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    13621 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    13681 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    13741 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    13801 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    13861 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    13921 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
    13981 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    14041 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    14101 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    14161 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    14221 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    14281 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    14341 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    14401 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    14461 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    14521 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    14581 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    14641 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    14701 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    14761 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    14821 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    14881 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    14941 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    15001 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    15061 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    15121 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    15181 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    15241 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc
    15301 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
    15361 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    15421 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    15481 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    15541 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    15601 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    15661 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    15721 aaaatattaa cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc
    15781 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
    15841 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
    15901 agcgcgccta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
    15961 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
    16021 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
    16081 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
    16141 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
    16201 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
    16261 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
    16321 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
    16381 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
    16441 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
    16501 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc tggctagatt
    16561 aagcgtctga tgagtccgtg aggacgaaac ccggcgtacc gggtc
//
