LOCUS       Exported               15894 bp DNA     circular SYN 06-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 7, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15894
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             3325..4044
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4087..5148
                     /codon_start=1
                     /label=ChIEF
                     /translation="MSRRPWLLALALAVALAAGSAGASTGSDATVPVATQDGPDYVFHR
                     AHERMLFQTSYTLENNGSVICIPNNGQCFCLAWLKSNGTNAEKLAANILQWITFALSAL
                     CLMFYGYQTWKSTCGWEEIYVATIEMIKFIIEYFHEFDEPAVIYSSNGNKTVWLRYAAW
                     LLTCPVVLIHLSNLTGLANDYNKRTMGLLVSDIGCIVWGTTAALSKGYVRVIFFLMGLC
                     YGIYTFFNAAKVYIEAYHTVPKGRCRQVVTGMAWLFFVSWGMFPILFILGPEGFGVLSV
                     YGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDIRKTTKLNIGGTEIEVETLVE
                     DEAEAGAVVNGSG"
     CDS             7323..7334
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(9126..9137)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      12348..12395
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12423..12439)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12447..12463
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12471..12501)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12516..12537
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(12825..13413)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(13584..14444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(14445..14549)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14576..15031
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     15172..15188
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15258..15561
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15562..15765
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        15810..15828
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatggtg agcaagggcg aggagctgtt caccggggtg
     3361 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3421 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3481 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     3541 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     3601 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     3661 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     3721 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     3781 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     3841 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     3901 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     3961 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4021 ggcatggacg agctgtacaa gtaagctaga catgaaaaaa actaacactc ctccggtacc
     4081 gccaccatgt cgcggaggcc atggcttctt gccctagcgc tggcagtggc gctggcggcc
     4141 ggcagcgcag gagcctcgac tggcagtgac gcgacggtgc cggtcgcgac tcaggatggc
     4201 cccgactacg ttttccaccg tgcccacgag cgcatgctct tccaaacctc atacactctt
     4261 gagaacaatg gttctgttat ttgcatcccg aacaacggcc agtgcttctg cttggcttgg
     4321 cttaaatcca acggaacaaa tgccgagaag ttggctgcca acattctgca gtggattact
     4381 tttgcgcttt cagcgctctg cctgatgttc tacggctacc agacctggaa gtctacttgc
     4441 ggctgggagg agatttacgt ggccacgatc gagatgatca agttcatcat cgagtatttc
     4501 catgagtttg acgaacctgc ggtgatctac tcatccaacg gcaacaagac cgtgtggctt
     4561 cgttacgcgg cctggctgct gacctgccct gtcgttctta tccatctgag caaccttacg
     4621 ggtctggcga acgactataa caagcgtacc atgggtctgc tggtgtcaga tatcggctgt
     4681 atcgtgtggg gcaccacggc cgcgctgtcc aagggatacg tccgtgtcat tttcttcctg
     4741 atgggcctgt gctacggcat ctacacattc ttcaacgcag ccaaggtcta cattgaggcg
     4801 taccacaccg tgccgaaggg ccggtgtcgc caggtggtga ctggcatggc ttggctcttc
     4861 ttcgtatcat ggggtatgtt ccccatcctg ttcatcctcg gccccgaggg cttcggcgtc
     4921 ctgagcgtgt acggctccac cgtcggccac accatcattg acctgatgtc gaagaactgc
     4981 tggggtctgc tcggccacta cctgcgcgtg ctgatccacg agcatatcct catccacggc
     5041 gacattcgca agaccaccaa attgaacatt ggtggcactg agattgaggt cgagacgctg
     5101 gtggaggacg aggccgaggc tggcgcggtc gttaacggaa gcggataagc tagcggcgcc
     5161 tagccggtca tccttttgac gattccagtc ccgaggataa cctcctctcg ggattggggg
     5221 gaatctttgg atccagtagt cctccttgaa ctccgtccaa cagggcagat tcaagagtca
     5281 taagactttc attaatcatt tcagttgatc agacatggtc gtgtagattc tcttaatacg
     5341 ggagatcttc tagcagtttc agtgaccaac ggtgctttca ttctccagga actgatacca
     5401 aaggttgtgg acaggccaag gggtacttcg gatgactctg tgcttgggca cagaaagagg
     5461 tcgtagtgtg ccccccgata gcggactcga catgaatcaa ctaagaaagg caatctgcct
     5521 cccatgatgg acataagcaa tagttcacaa ccatcttgca tctcagtgaa gtgtacataa
     5581 ctattgaggg ctgggtcatc taagcatttc agtcgagaaa aaaactgtag accaaaagaa
     5641 caactagcaa cacttctcat ccagagaccc atatcaagat gctagatccg ggagaggttt
     5701 atgatgaccc tattgatcca attgagtcag aggctgaacc cagaggaacc cccactgtcc
     5761 ccaacatctt gaggaactcc gactacaatc tcaattctcc tttgatagag gatcctgcca
     5821 aactaatgtt agaatggttg aagacaggga acagacctta tcggatgact ttgacagaca
     5881 attgctccag gtcttacaaa gttttgaaag attatttcaa gaaagtagat ttgggttctc
     5941 tcaaagtggg cggaactgct gcacagtcaa tggtttctct ctggttgtgt ggtgcccact
     6001 ctgaatcaaa caggagccgg agatgtataa ccgacttggc ccatttctat tccaagtcat
     6061 cccccataga gaagctattg aattgtacgc taggaaacag aggcctgaga atcccaccag
     6121 agggggtgtt aaattgcctc gagagggtca attatgacaa ggcatttggg aggtatctgg
     6181 ccaacacgta ttcctcttac ttgtttttcc atgtaatcac cttatacatg aatgccttag
     6241 actgggaaga ggaaaagacc atcctggcat tatggaaaga tataacctca gtggataccg
     6301 agaaggactt ggtcaaattc aaagatcaaa tatggggact gttgattgtg acaaaggact
     6361 ttgtttactc tcagagttca aactgtcttt ttgacagaaa ctacacactg atgctaaagg
     6421 atcttttctt gtctcgattc aactccttaa tgattctgct ttctccccct gagccccgat
     6481 actcagatga cttaatatct cagctgtgcc agctatacat cgctggggat caagtcttgt
     6541 ccatgtgtgg gaactccggc tatgaagtca tcaaaatatt ggagccatat gtcgtgaaca
     6601 gtttggtcca gagggcagag aagtttaggc ctctcatcca ccccttggga gactttccta
     6661 tgttcataaa agacaaggtg aatcaacttg aagggacttt tggtcccagt gcaaagaggt
     6721 tttttagggt tttagatcaa ttcgacaaca tacatgactt agtatttgtg tatggctgtt
     6781 acagacattg ggggcacccc tatatagatt atcggaaggg tctgtcgaaa ctatatgatc
     6841 aagttcacat taagaaagta atagataagt cctaccagga gtgtttagca agtgacttgg
     6901 ccagaaggat cctcagatgg ggatttgaca agtactccaa atggtatcta gattcgagat
     6961 tccttgcccg agaccacccc ttgactcctt atgtcaagac ccaaacatgg ccacccaaac
     7021 atatagtaga cttggtgggg gacacatggc ataagctccc gatcacgcag atctttgaaa
     7081 ttcctgaatc aatggacccg tcagagatac tggatgacaa atcacattct ttcaccagaa
     7141 caaggttagc ttcttggctg tcagagaacc gaggggggcc tgttcctagc gagaaggtca
     7201 ttatcacggc cctgtctaag ccacctgtca atccccgaga gtttttgaaa tctatagacc
     7261 tcggaggatt gccagatgag gatttgataa ttggcctcaa accaaaggaa cgggagttga
     7321 agatcgaggg ccgattcttt gctctaatgt catggaatct aagattatat tttgtcatca
     7381 ccgaaaagct cttggccaac tacattttgc cactttttga cgcactgact atgacagaca
     7441 acctgaacaa ggtgttcaaa aagttgatcg acagggtcac cgggcaaggg cttttggact
     7501 attctagggt cacatacgca tttcacctgg actatgagaa atggaacaat catcaaagat
     7561 tggagtcaac agaggatgta ttctctgtcc tagatcaggt gtttggattg aagagggtgt
     7621 tttctagaac acacgagttt tttcagaagt cctggatcta ttattcagac agatcagacc
     7681 tcattgggtt atgggaggac caaatatatt gcttggatat gtctaacggc ccaacctgct
     7741 ggaatggcca agatggcggg ctagagggct tgcggcagaa gggctggagt ctagtcagtt
     7801 tattaatgat agatagagaa tctcaaacca ggaacacaag aaccaagata ctagctcaag
     7861 gagacaacca ggttctgtgt cctacataca tgttgtcacc gggattgtct caagaggggc
     7921 ttctctatga gttagagagc atatcaagga atgcactctc aatataccga gctatcgagg
     7981 aaggagcatc taagctgggg ctgatcatca agaaggaaga gaccatgtgt agttatgact
     8041 ttctcatata tgggaagacc cccttatttc gaggcaacat attggtgcct gaatccaaaa
     8101 gatgggcccg agtctcttgc atctctaacg accaaatagt caacctcgcc aatataatgt
     8161 cgacagtatc caccaatgcg ctgacagtgg cacaacactc tcaatctctg atcaaaccta
     8221 tgagggattt tctgctcatg tcagtacagg cagttttcca ctacctgttg tttagcccaa
     8281 tcctaaaagg cagagtttat aagattctga gtgctgaagg ggagagcttt ctcctagcca
     8341 tgtcgcggat aatctaccta gatccttctt tgggaggggt gtctggaatg tctctcggga
     8401 ggttccatat acgtcagttc tcagaccctg tctctgaagg gttgtcattc tggagagaga
     8461 tctggttagg ctctcatgag tcctggattc acgcgttgtg tcaggaggcc gggaaccccg
     8521 atcttggaga gagaacactc gagagcttca ctcgcctttt agaagatcct actaccttaa
     8581 atatcaaagg aggggccagc cctaccattc tactcaagga tgctatcaga aaggctctgt
     8641 acgacgaggt ggacaaggtg gaaaattcag agtttcgaga ggcaatcctg ctgtccaaga
     8701 cccatagaga taactttata ctctttttaa aatctgttga gcctctgttc cctcgatttc
     8761 tcagtgagct cttcagttcg tctttcttgg gaataccgga gtcaatcatt ggactgatac
     8821 aaaactcccg gacaataaga aggcagttta gaaagagtct ctcaagaact ttagaagagt
     8881 ccttctacaa ctcagagatc cacgggatta atcggataac ccagacacct caaagggtcg
     8941 gaagggtgtg gccttgctct tcagagaggg cggatctact tagggagatc tcttggggga
     9001 ggaaagtggt aggcacgaca gttcctcacc cttccgagat gttggagttg tttcccaaat
     9061 cctccatttc ctgcacttgt ggagcaacag ggggaggcaa tcctagagtc tctgtatcag
     9121 tactcccgtc cttcgatcag tcatttttct cacggggccc cctaaaggga tacttgggct
     9181 cgtccacctc tatgtcaacc cagctattcc atgcatggga aaaagtcact aatgttcatg
     9241 tggtgaaaag ggctctatcg ttaaaagaat ctataaactg gttcatcaat aggaattcca
     9301 atttggctca aactctaatt ggaaacatca tgtctctgac aggccctgac ttccctctag
     9361 aagaggcccc tgttttcaaa cggacagggt cagccttgca taggttcaag tctgccagat
     9421 acagcgaagg agggtattct tctgtttgcc ctaaccttct ctcccatatc tctgttagta
     9481 cagacactat gtctgatttg acccaaaacg ggaagaacta tgatttcatg tttcagccat
     9541 tgatgcttta tgcgcaaaca tggacatcgg aactggtaca gagggataca agacttagag
     9601 actccacgtt tcactggcac cttcggtgca acagatgtgt gaggcccatt gatgatataa
     9661 cactggaaac ttctcagatc ttcgagttcc cggatgtgtc aaaaaggata tccaggatgg
     9721 tttctggagc tgtccctcaa tttcagaagc ttcctgatat ccgtctaaga ccaggtgatt
     9781 ttgaatccct aagtggtaga gaaaagtctc gccatatagg gtcagctcag gggctcttat
     9841 actcaatctt agtagcaatt cacgactcag gatacaatga tgggaccatc ttccctgtca
     9901 acatatacgg caaagtttcc cccagagact atttgagagg gcttgcaaga gggatcttga
     9961 tagggtcctc gatttgcttc ttgacacgaa tgacaaatat taacattaac agacctcttg
    10021 aattgatctc aggggtaatt tcctatattc tcctgaggct ggataatcat ccctctctgt
    10081 atataatgct cagagaaccg tctcttagag gagaaatatt ctctatccct cagaaaatcc
    10141 ccgccgctta cccaaccact atgagagaag gcaacagatc gattttgtgt tacctccaac
    10201 acgtgctacg ctatgagcga gaggcaatca cggcgtcccc ggagaatgac tggctgtgga
    10261 tcttctcaga cttcagaagt gtgaaaatga cgtacttgac cctcattact taccagtctc
    10321 acctcctact ccagagggtt gagagaaact tgtctaagag tatgagagct actctgcgac
    10381 aaatgggttc cttaatgagg caagtgctgg gtgggcacgg agaagatacc ttggagtcag
    10441 acgatgacat tcaacgatta ctaaaagact ctttgcgaaa gacaaggtgg gtggatcaag
    10501 aggtgcgcca tgcagctaga accatgaatg gagattacag ccccgacaag aaagtatccc
    10561 gtaaggtagg atgttcagaa tgggtctgct ctgctcaaca gattgccgtc tccacctcag
    10621 ccaacccggc ccctgtctca gagcttgaca ttagggccct ctctaagcgg tttcaaaacc
    10681 ccttgatctc gggcctgaga gtggttcagt gggcaaccgg tgcccattat aagcttaagc
    10741 ctattctaga tgatctaaat gttttcccat ctctctgtct tgtaattgga gacgggtcag
    10801 ggggaatatc aagggcagtt ctcaacatgt ttccagattc taagcttgtg ttcaacagcc
    10861 tattggaggt gaatgatctg atggcttccg gaacacatcc actgcctcct tcagcaatca
    10921 tgagtggagg agatgacatc atctccagag tgatagactt tgactcaata tgggagaaac
    10981 cgtccgacct gaggaactcg gccacctgga gatacttcca gtcggttcaa aaacaggtca
    11041 acatgtcgta tgacctcatt atttgtgatg cagaagttac tgatattgca tctatcaacc
    11101 ggataactct gttgatgtct gatttcgcat tgtctataga tggaccactt tatctggtct
    11161 tcaaaactta cgggactatg ctagtaaacc cggactataa agctattcaa catctgtcaa
    11221 gagcgttccc ttcggtcaca gggtttgtaa cccaagtaac ttcatccttt tcttctgagc
    11281 tatacctccg gttctctaaa cgaggaaagt ttttcaggga cgccgagtac ttgacctctt
    11341 ccacccttcg agagatgagc cttgtgttgt tcaattgcag cagccccaaa agtgagatgc
    11401 agagagctcg ttccttaaac tatcaagacc tggtaagggg atttcctgaa gagatcatat
    11461 caaatcctta caacgaaatg atcataactc tgattgacaa tgatgtagag tccttcctag
    11521 tccacaagat ggtggatgat cttgagctac agaggggaac tctgtctaaa gtggctatca
    11581 ttatatccat catgatcgtt ttttccaata gagtcttcaa catttccaaa cctttgactg
    11641 accccttgtt ctatccaccg tctgatccta aaatcctgag gcacttcaac atatgttgca
    11701 gtactatgat gtatctatct accgctttag gcgacgtccc taacttcgca agacttcatg
    11761 acctgtataa cagacctata acttgttact tcagaaaaca agttattcga gggaatattt
    11821 atctatcttg gagttggtcc gatgataccc cagtgttcaa gagagtagcc tgtaattcta
    11881 gcttgagtct gtcatctcac tggatcaggt tgatctacaa gatagtgaag actaccagac
    11941 tcattggcag cataaaagac ctatcaggag aggtagaacg acatcttcat gggtataaca
    12001 gatggatcac cctcgaggat atccgatcta gatcatccct actagattac agttgtttgt
    12061 aagccggata ttaccgaaag cctgtgcatg ctaaaattct tgtatgatgc atcttgaaaa
    12121 aaacaagatc ttgaatccgg acctctggtt gtttgattgt tttttccatc tttattgttt
    12181 ttttgttaag cgtgggtcgg catggcatct ccacctcctc gcggtccgac ctgggcatcc
    12241 gaaggaggac gcacgtccac tcggatggct aagggagggg cggggatccg gctgctaaca
    12301 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
    12361 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa agtcgcgctt ggcgtaatca
    12421 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    12481 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    12541 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    12601 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    12661 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    12721 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    12781 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    12841 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    12901 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    12961 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    13021 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    13081 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    13141 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    13201 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    13261 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    13321 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    13381 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    13441 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    13501 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    13561 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    13621 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    13681 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    13741 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    13801 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    13861 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    13921 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    13981 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    14041 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    14101 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    14161 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    14221 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    14281 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    14341 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    14401 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    14461 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    14521 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
    14581 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    14641 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    14701 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    14761 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    14821 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    14881 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    14941 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    15001 aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt
    15061 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
    15121 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
    15181 cggccagtga gcgcgcctag ttattaatag taatcaatta cggggtcatt agttcatagc
    15241 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
    15301 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
    15361 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
    15421 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
    15481 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
    15541 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
    15601 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
    15661 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
    15721 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga
    15781 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
    15841 ggctagatta agcgtctgat gagtccgtga ggacgaaacc cggcgtaccg ggtc
//
