LOCUS       Exported               16108 bp DNA     circular SYN 07-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 7, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16108
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             3325..4044
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4091..5362
                     /codon_start=1
                     /label=FlpO
                     /translation="MSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     CDS             7537..7548
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(9340..9351)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      12562..12609
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12637..12653)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12661..12677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12685..12715)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12730..12751
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(13039..13627)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(13798..14658)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(14659..14763)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14790..15245
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     15386..15402
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15472..15775
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15776..15979
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        16024..16042
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatggtg agcaagggcg aggagctgtt caccggggtg
     3361 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3421 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3481 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     3541 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     3601 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     3661 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     3721 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     3781 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     3841 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     3901 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     3961 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4021 ggcatggacg agctgtacaa gtaagctaga catgaaaaaa actaacactc ctccggtacc
     4081 ggatcccacc atgagccagt tcgacatcct gtgcaagacc ccccccaagg tgctggtgcg
     4141 gcagttcgtg gagagattcg agaggcccag cggcgagaag atcgccagct gtgccgccga
     4201 gctgacctac ctgtgctgga tgatcaccca caacggcacc gccatcaaga gggccacctt
     4261 catgagctac aacaccatca tcagcaacag cctgagcttc gacatcgtga acaagagcct
     4321 gcagttcaag tacaagaccc agaaggccac catcctggag gccagcctga agaagctgat
     4381 ccccgcctgg gagttcacca tcatccctta caacggccag aagcaccaga gcgacatcac
     4441 cgacatcgtg tccagcctgc agctgcagtt cgagagcagc gaggaggccg acaagggcaa
     4501 cagccacagc aagaagatgc tgaaggccct gctgtccgag ggcgagagca tctgggagat
     4561 caccgagaag atcctgaaca gcttcgagta caccagcagg ttcaccaaga ccaagaccct
     4621 gtaccagttc ctgttcctgg ccacattcat caactgcggc aggttcagcg acatcaagaa
     4681 cgtggacccc aagagcttca agctggtgca gaacaagtac ctgggcgtga tcattcagtg
     4741 cctggtgacc gagaccaaga caagcgtgtc caggcacatc tactttttca gcgccagagg
     4801 caggatcgac cccctggtgt acctggacga gttcctgagg aacagcgagc ccgtgctgaa
     4861 gagagtgaac aggaccggca acagcagcag caacaagcag gagtaccagc tgctgaagga
     4921 caacctggtg cgcagctaca acaaggccct gaagaagaac gccccctacc ccatcttcgc
     4981 tatcaagaac ggccctaaga gccacatcgg caggcacctg atgaccagct ttctgagcat
     5041 gaagggcctg accgagctga caaacgtggt gggcaactgg agcgacaaga gggcctccgc
     5101 cgtggccagg accacctaca cccaccagat caccgccatc cccgaccact acttcgccct
     5161 ggtgtccagg tactacgcct acgaccccat cagcaaggag atgatcgccc tgaaggacga
     5221 gaccaacccc atcgaggagt ggcagcacat cgagcagctg aagggcagcg ccgagggcag
     5281 catcagatac cccgcctgga acggcatcat cagccaggag gtgctggact acctgagcag
     5341 ctacatcaac aggcggatct gagctagcgg cgcctagccg gtcatccttt tgacgattcc
     5401 agtcccgagg ataacctcct ctcgggattg gggggaatct ttggatccag tagtcctcct
     5461 tgaactccgt ccaacagggc agattcaaga gtcataagac tttcattaat catttcagtt
     5521 gatcagacat ggtcgtgtag attctcttaa tacgggagat cttctagcag tttcagtgac
     5581 caacggtgct ttcattctcc aggaactgat accaaaggtt gtggacaggc caaggggtac
     5641 ttcggatgac tctgtgcttg ggcacagaaa gaggtcgtag tgtgcccccc gatagcggac
     5701 tcgacatgaa tcaactaaga aaggcaatct gcctcccatg atggacataa gcaatagttc
     5761 acaaccatct tgcatctcag tgaagtgtac ataactattg agggctgggt catctaagca
     5821 tttcagtcga gaaaaaaact gtagaccaaa agaacaacta gcaacacttc tcatccagag
     5881 acccatatca agatgctaga tccgggagag gtttatgatg accctattga tccaattgag
     5941 tcagaggctg aacccagagg aacccccact gtccccaaca tcttgaggaa ctccgactac
     6001 aatctcaatt ctcctttgat agaggatcct gccaaactaa tgttagaatg gttgaagaca
     6061 gggaacagac cttatcggat gactttgaca gacaattgct ccaggtctta caaagttttg
     6121 aaagattatt tcaagaaagt agatttgggt tctctcaaag tgggcggaac tgctgcacag
     6181 tcaatggttt ctctctggtt gtgtggtgcc cactctgaat caaacaggag ccggagatgt
     6241 ataaccgact tggcccattt ctattccaag tcatccccca tagagaagct attgaattgt
     6301 acgctaggaa acagaggcct gagaatccca ccagaggggg tgttaaattg cctcgagagg
     6361 gtcaattatg acaaggcatt tgggaggtat ctggccaaca cgtattcctc ttacttgttt
     6421 ttccatgtaa tcaccttata catgaatgcc ttagactggg aagaggaaaa gaccatcctg
     6481 gcattatgga aagatataac ctcagtggat accgagaagg acttggtcaa attcaaagat
     6541 caaatatggg gactgttgat tgtgacaaag gactttgttt actctcagag ttcaaactgt
     6601 ctttttgaca gaaactacac actgatgcta aaggatcttt tcttgtctcg attcaactcc
     6661 ttaatgattc tgctttctcc ccctgagccc cgatactcag atgacttaat atctcagctg
     6721 tgccagctat acatcgctgg ggatcaagtc ttgtccatgt gtgggaactc cggctatgaa
     6781 gtcatcaaaa tattggagcc atatgtcgtg aacagtttgg tccagagggc agagaagttt
     6841 aggcctctca tccacccctt gggagacttt cctatgttca taaaagacaa ggtgaatcaa
     6901 cttgaaggga cttttggtcc cagtgcaaag aggtttttta gggttttaga tcaattcgac
     6961 aacatacatg acttagtatt tgtgtatggc tgttacagac attgggggca cccctatata
     7021 gattatcgga agggtctgtc gaaactatat gatcaagttc acattaagaa agtaatagat
     7081 aagtcctacc aggagtgttt agcaagtgac ttggccagaa ggatcctcag atggggattt
     7141 gacaagtact ccaaatggta tctagattcg agattccttg cccgagacca ccccttgact
     7201 ccttatgtca agacccaaac atggccaccc aaacatatag tagacttggt gggggacaca
     7261 tggcataagc tcccgatcac gcagatcttt gaaattcctg aatcaatgga cccgtcagag
     7321 atactggatg acaaatcaca ttctttcacc agaacaaggt tagcttcttg gctgtcagag
     7381 aaccgagggg ggcctgttcc tagcgagaag gtcattatca cggccctgtc taagccacct
     7441 gtcaatcccc gagagttttt gaaatctata gacctcggag gattgccaga tgaggatttg
     7501 ataattggcc tcaaaccaaa ggaacgggag ttgaagatcg agggccgatt ctttgctcta
     7561 atgtcatgga atctaagatt atattttgtc atcaccgaaa agctcttggc caactacatt
     7621 ttgccacttt ttgacgcact gactatgaca gacaacctga acaaggtgtt caaaaagttg
     7681 atcgacaggg tcaccgggca agggcttttg gactattcta gggtcacata cgcatttcac
     7741 ctggactatg agaaatggaa caatcatcaa agattggagt caacagagga tgtattctct
     7801 gtcctagatc aggtgtttgg attgaagagg gtgttttcta gaacacacga gttttttcag
     7861 aagtcctgga tctattattc agacagatca gacctcattg ggttatggga ggaccaaata
     7921 tattgcttgg atatgtctaa cggcccaacc tgctggaatg gccaagatgg cgggctagag
     7981 ggcttgcggc agaagggctg gagtctagtc agtttattaa tgatagatag agaatctcaa
     8041 accaggaaca caagaaccaa gatactagct caaggagaca accaggttct gtgtcctaca
     8101 tacatgttgt caccgggatt gtctcaagag gggcttctct atgagttaga gagcatatca
     8161 aggaatgcac tctcaatata ccgagctatc gaggaaggag catctaagct ggggctgatc
     8221 atcaagaagg aagagaccat gtgtagttat gactttctca tatatgggaa gaccccctta
     8281 tttcgaggca acatattggt gcctgaatcc aaaagatggg cccgagtctc ttgcatctct
     8341 aacgaccaaa tagtcaacct cgccaatata atgtcgacag tatccaccaa tgcgctgaca
     8401 gtggcacaac actctcaatc tctgatcaaa cctatgaggg attttctgct catgtcagta
     8461 caggcagttt tccactacct gttgtttagc ccaatcctaa aaggcagagt ttataagatt
     8521 ctgagtgctg aaggggagag ctttctccta gccatgtcgc ggataatcta cctagatcct
     8581 tctttgggag gggtgtctgg aatgtctctc gggaggttcc atatacgtca gttctcagac
     8641 cctgtctctg aagggttgtc attctggaga gagatctggt taggctctca tgagtcctgg
     8701 attcacgcgt tgtgtcagga ggccgggaac cccgatcttg gagagagaac actcgagagc
     8761 ttcactcgcc ttttagaaga tcctactacc ttaaatatca aaggaggggc cagccctacc
     8821 attctactca aggatgctat cagaaaggct ctgtacgacg aggtggacaa ggtggaaaat
     8881 tcagagtttc gagaggcaat cctgctgtcc aagacccata gagataactt tatactcttt
     8941 ttaaaatctg ttgagcctct gttccctcga tttctcagtg agctcttcag ttcgtctttc
     9001 ttgggaatac cggagtcaat cattggactg atacaaaact cccggacaat aagaaggcag
     9061 tttagaaaga gtctctcaag aactttagaa gagtccttct acaactcaga gatccacggg
     9121 attaatcgga taacccagac acctcaaagg gtcggaaggg tgtggccttg ctcttcagag
     9181 agggcggatc tacttaggga gatctcttgg gggaggaaag tggtaggcac gacagttcct
     9241 cacccttccg agatgttgga gttgtttccc aaatcctcca tttcctgcac ttgtggagca
     9301 acagggggag gcaatcctag agtctctgta tcagtactcc cgtccttcga tcagtcattt
     9361 ttctcacggg gccccctaaa gggatacttg ggctcgtcca cctctatgtc aacccagcta
     9421 ttccatgcat gggaaaaagt cactaatgtt catgtggtga aaagggctct atcgttaaaa
     9481 gaatctataa actggttcat caataggaat tccaatttgg ctcaaactct aattggaaac
     9541 atcatgtctc tgacaggccc tgacttccct ctagaagagg cccctgtttt caaacggaca
     9601 gggtcagcct tgcataggtt caagtctgcc agatacagcg aaggagggta ttcttctgtt
     9661 tgccctaacc ttctctccca tatctctgtt agtacagaca ctatgtctga tttgacccaa
     9721 aacgggaaga actatgattt catgtttcag ccattgatgc tttatgcgca aacatggaca
     9781 tcggaactgg tacagaggga tacaagactt agagactcca cgtttcactg gcaccttcgg
     9841 tgcaacagat gtgtgaggcc cattgatgat ataacactgg aaacttctca gatcttcgag
     9901 ttcccggatg tgtcaaaaag gatatccagg atggtttctg gagctgtccc tcaatttcag
     9961 aagcttcctg atatccgtct aagaccaggt gattttgaat ccctaagtgg tagagaaaag
    10021 tctcgccata tagggtcagc tcaggggctc ttatactcaa tcttagtagc aattcacgac
    10081 tcaggataca atgatgggac catcttccct gtcaacatat acggcaaagt ttcccccaga
    10141 gactatttga gagggcttgc aagagggatc ttgatagggt cctcgatttg cttcttgaca
    10201 cgaatgacaa atattaacat taacagacct cttgaattga tctcaggggt aatttcctat
    10261 attctcctga ggctggataa tcatccctct ctgtatataa tgctcagaga accgtctctt
    10321 agaggagaaa tattctctat ccctcagaaa atccccgccg cttacccaac cactatgaga
    10381 gaaggcaaca gatcgatttt gtgttacctc caacacgtgc tacgctatga gcgagaggca
    10441 atcacggcgt ccccggagaa tgactggctg tggatcttct cagacttcag aagtgtgaaa
    10501 atgacgtact tgaccctcat tacttaccag tctcacctcc tactccagag ggttgagaga
    10561 aacttgtcta agagtatgag agctactctg cgacaaatgg gttccttaat gaggcaagtg
    10621 ctgggtgggc acggagaaga taccttggag tcagacgatg acattcaacg attactaaaa
    10681 gactctttgc gaaagacaag gtgggtggat caagaggtgc gccatgcagc tagaaccatg
    10741 aatggagatt acagccccga caagaaagta tcccgtaagg taggatgttc agaatgggtc
    10801 tgctctgctc aacagattgc cgtctccacc tcagccaacc cggcccctgt ctcagagctt
    10861 gacattaggg ccctctctaa gcggtttcaa aaccccttga tctcgggcct gagagtggtt
    10921 cagtgggcaa ccggtgccca ttataagctt aagcctattc tagatgatct aaatgttttc
    10981 ccatctctct gtcttgtaat tggagacggg tcagggggaa tatcaagggc agttctcaac
    11041 atgtttccag attctaagct tgtgttcaac agcctattgg aggtgaatga tctgatggct
    11101 tccggaacac atccactgcc tccttcagca atcatgagtg gaggagatga catcatctcc
    11161 agagtgatag actttgactc aatatgggag aaaccgtccg acctgaggaa ctcggccacc
    11221 tggagatact tccagtcggt tcaaaaacag gtcaacatgt cgtatgacct cattatttgt
    11281 gatgcagaag ttactgatat tgcatctatc aaccggataa ctctgttgat gtctgatttc
    11341 gcattgtcta tagatggacc actttatctg gtcttcaaaa cttacgggac tatgctagta
    11401 aacccggact ataaagctat tcaacatctg tcaagagcgt tcccttcggt cacagggttt
    11461 gtaacccaag taacttcatc cttttcttct gagctatacc tccggttctc taaacgagga
    11521 aagtttttca gggacgccga gtacttgacc tcttccaccc ttcgagagat gagccttgtg
    11581 ttgttcaatt gcagcagccc caaaagtgag atgcagagag ctcgttcctt aaactatcaa
    11641 gacctggtaa ggggatttcc tgaagagatc atatcaaatc cttacaacga aatgatcata
    11701 actctgattg acaatgatgt agagtccttc ctagtccaca agatggtgga tgatcttgag
    11761 ctacagaggg gaactctgtc taaagtggct atcattatat ccatcatgat cgttttttcc
    11821 aatagagtct tcaacatttc caaacctttg actgacccct tgttctatcc accgtctgat
    11881 cctaaaatcc tgaggcactt caacatatgt tgcagtacta tgatgtatct atctaccgct
    11941 ttaggcgacg tccctaactt cgcaagactt catgacctgt ataacagacc tataacttgt
    12001 tacttcagaa aacaagttat tcgagggaat atttatctat cttggagttg gtccgatgat
    12061 accccagtgt tcaagagagt agcctgtaat tctagcttga gtctgtcatc tcactggatc
    12121 aggttgatct acaagatagt gaagactacc agactcattg gcagcataaa agacctatca
    12181 ggagaggtag aacgacatct tcatgggtat aacagatgga tcaccctcga ggatatccga
    12241 tctagatcat ccctactaga ttacagttgt ttgtaagccg gatattaccg aaagcctgtg
    12301 catgctaaaa ttcttgtatg atgcatcttg aaaaaaacaa gatcttgaat ccggacctct
    12361 ggttgtttga ttgttttttc catctttatt gtttttttgt taagcgtggg tcggcatggc
    12421 atctccacct cctcgcggtc cgacctgggc atccgaagga ggacgcacgt ccactcggat
    12481 ggctaaggga ggggcgggga tccggctgct aacaaagccc gaaaggaagc tgagttggct
    12541 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
    12601 ggttttttgc tgaaagtcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    12661 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    12721 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    12781 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    12841 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    12901 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    12961 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    13021 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    13081 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    13141 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    13201 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    13261 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    13321 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    13381 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    13441 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
    13501 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    13561 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    13621 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    13681 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    13741 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    13801 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    13861 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    13921 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    13981 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    14041 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    14101 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    14161 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    14221 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    14281 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    14341 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    14401 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    14461 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    14521 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    14581 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    14641 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    14701 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    14761 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc
    14821 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
    14881 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
    14941 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
    15001 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
    15061 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
    15121 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
    15181 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
    15241 aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
    15301 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
    15361 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc ctagttatta
    15421 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
    15481 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
    15541 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
    15601 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
    15661 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
    15721 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
    15781 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
    15841 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
    15901 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
    15961 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
    16021 aattaatacg actcactata gggagaccca agctggctag attaagcgtc tgatgagtcc
    16081 gtgaggacga aacccggcgt accgggtc
//
