LOCUS       Exported               16617 bp DNA     circular SYN 07-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 7, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16617
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             71..1423
                     /codon_start=1
                     /label=N
                     /translation="MDADKIVFKVNNQVVSLKPEIIVDQYEYKYPAIKDLKKPCITLGK
                     APDLNKAYKSVLSGMNAAKLDPDDVCSYLAAAMQFFEGTCPEDWTSYGILIARKGDRIT
                     PNSLVEIKRTDVEGNWALTGGMELTRDPTVSEHASLVGLLLSLYRLSKISGQNTGNYKT
                     NIADRIEQIFETAPFVKIVEHHTLMTTHKMCANWSTIPNFRFLAGTYDMFFSRIEHLYS
                     AIRVGTVVTAYEDCSGLVSFTGFIKQINLTAREAILYFFHKNFEEEIRRMFEPGQETAV
                     PHSYFIHFRSLGLSGKSPYSSNAVGHVFNLIHFVGCYMGQVRSLNATVIAACAPHEMSV
                     LGGYLGEEFFGKGTFERRFFRDEKELQEYEAAELTKSDVALADDGTVNSDDEDYFSGET
                     RSPEAVYTRIMMNGGRLKRSHIRRYVSVSSNHQARPNSFAEFLNKTYSNDS"
     CDS             1514..2407
                     /codon_start=1
                     /label=P
                     /translation="MSKIFVNPSAIRAGLADLEMAEETVDLINRNIEDNQAHLQGEPIE
                     VDNLPEDMKRLHLDDEKSSNLGEMVRVGEGKYREDFQMDEGEDPNLLFQSYLDNVGVQI
                     VRQMRSGERFLKIWSQTVEEIVSYVTVNFPNPPRRSSEDKSTQTTGRELKKETTSAFSQ
                     RESQPSKARMVAQVAPGPPALEWSATNEEDDLSVEAEIAHQIAESFSKKYKFPSRSSGI
                     FLYNFEQLKMNLDDIVKEAKNVPGVTRLAHDGSKIPLRCVLGWVALANSKKFQLLVEAD
                     KLSKIMQDDLNRYTSC"
     CDS             2496..3104
                     /codon_start=1
                     /label=M
                     /translation="MNVLRKIVKKCRDEDTQKPSPVSAPPYDDDLWLPPPEYVPLKELT
                     SKKNMRNFCVNGEVKACSPNGYSFRILRHILRSFNEIYSGNHRMIGLVKIVVGLALSGA
                     PVPEGMNWVYKLRRTLIFQWADSRGPLEGEELDYSQEITWDDDTEFVGLQIRVSARQCH
                     IQGRIWCINTNSRACQLWSDMSLQTQRSEEDKDSSLLLE"
     CDS             3325..4755
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             4798..5868
                     /codon_start=1
                     /label=iCre
                     /translation="MVPKKKRKVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAF
                     SEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLN
                     MLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRC
                     QDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALS
                     LGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIY
                     GAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSET
                     GAMVRLLEDGDGSGGSG"
     CDS             4804..4824
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6402..12785
                     /codon_start=1
                     /label=L
                     /translation="MLDPGEVYDDPIDPIESEAEPRGTPTVPNILRNSDYNLNSPLIED
                     PAKLMLEWLKTGNRPYRMTLTDNCSRSYKVLKDYFKKVDLGSLKVGGTAAQSMVSLWLC
                     GAHSESNRSRRCITDLAHFYSKSSPIEKLLNCTLGNRGLRIPPEGVLNCLERVNYDKAF
                     GRYLANTYSSYLFFHVITLYMNALDWEEEKTILALWKDITSVDTEKDLVKFKDQIWGLL
                     IVTKDFVYSQSSNCLFDRNYTLMLKDLFLSRFNSLMILLSPPEPRYSDDLISQLCQLYI
                     AGDQVLSMCGNSGYEVIKILEPYVVNSLVQRAEKFRPLIHPLGDFPMFIKDKVNQLEGT
                     FGPSAKRFFRVLDQFDNIHDLVFVYGCYRHWGHPYIDYRKGLSKLYDQVHIKKVIDKSY
                     QECLASDLARRILRWGFDKYSKWYLDSRFLARDHPLTPYVKTQTWPPKHIVDLVGDTWH
                     KLPITQIFEIPESMDPSEILDDKSHSFTRTRLASWLSENRGGPVPSEKVIITALSKPPV
                     NPREFLKSIDLGGLPDEDLIIGLKPKERELKIEGRFFALMSWNLRLYFVITEKLLANYI
                     LPLFDALTMTDNLNKVFKKLIDRVTGQGLLDYSRVTYAFHLDYEKWNNHQRLESTEDVF
                     SVLDQVFGLKRVFSRTHEFFQKSWIYYSDRSDLIGLWEDQIYCLDMSNGPTCWNGQDGG
                     LEGLRQKGWSLVSLLMIDRESQTRNTRTKILAQGDNQVLCPTYMLSPGLSQEGLLYELE
                     SISRNALSIYRAIEEGASKLGLIIKKEETMCSYDFLIYGKTPLFRGNILVPESKRWARV
                     SCISNDQIVNLANIMSTVSTNALTVAQHSQSLIKPMRDFLLMSVQAVFHYLLFSPILKG
                     RVYKILSAEGESFLLAMSRIIYLDPSLGGVSGMSLGRFHIRQFSDPVSEGLSFWREIWL
                     GSHESWIHALCQEAGNPDLGERTLESFTRLLEDPTTLNIKGGASPTILLKDAIRKALYD
                     EVDKVENSEFREAILLSKTHRDNFILFLKSVEPLFPRFLSELFSSSFLGIPESIIGLIQ
                     NSRTIRRQFRKSLSRTLEESFYNSEIHGINRITQTPQRVGRVWPCSSERADLLREISWG
                     RKVVGTTVPHPSEMLELFPKSSISCTCGATGGGNPRVSVSVLPSFDQSFFSRGPLKGYL
                     GSSTSMSTQLFHAWEKVTNVHVVKRALSLKESINWFINRNSNLAQTLIGNIMSLTGPDF
                     PLEEAPVFKRTGSALHRFKSARYSEGGYSSVCPNLLSHISVSTDTMSDLTQNGKNYDFM
                     FQPLMLYAQTWTSELVQRDTRLRDSTFHWHLRCNRCVRPIDDITLETSQIFEFPDVSKR
                     ISRMVSGAVPQFQKLPDIRLRPGDFESLSGREKSRHIGSAQGLLYSILVAIHDSGYNDG
                     TIFPVNIYGKVSPRDYLRGLARGILIGSSICFLTRMTNININRPLELISGVISYILLRL
                     DNHPSLYIMLREPSLRGEIFSIPQKIPAAYPTTMREGNRSILCYLQHVLRYEREAITAS
                     PENDWLWIFSDFRSVKMTYLTLITYQSHLLLQRVERNLSKSMRATLRQMGSLMRQVLGG
                     HGEDTLESDDDIQRLLKDSLRKTRWVDQEVRHAARTMNGDYSPDKKVSRKVGCSEWVCS
                     AQQIAVSTSANPAPVSELDIRALSKRFQNPLISGLRVVQWATGAHYKLKPILDDLNVFP
                     SLCLVIGDGSGGISRAVLNMFPDSKLVFNSLLEVNDLMASGTHPLPPSAIMSGGDDIIS
                     RVIDFDSIWEKPSDLRNSATWRYFQSVQKQVNMSYDLIICDAEVTDIASINRITLLMSD
                     FALSIDGPLYLVFKTYGTMLVNPDYKAIQHLSRAFPSVTGFVTQVTSSFSSELYLRFSK
                     RGKFFRDAEYLTSSTLREMSLVLFNCSSPKSEMQRARSLNYQDLVRGFPEEIISNPYNE
                     MIITLIDNDVESFLVHKMVDDLELQRGTLSKVAIIISIMIVFSNRVFNISKPLTDPLFY
                     PPSDPKILRHFNICCSTMMYLSTALGDVPNFARLHDLYNRPITCYFRKQVIRGNIYLSW
                     SWSDDTPVFKRVACNSSLSLSSHWIRLIYKIVKTTRLIGSIKDLSGEVERHLHGYNRWI
                     TLEDIRSRSSLLDYSCL"
     CDS             8046..8057
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(9849..9860)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      13071..13118
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(13146..13162)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    13170..13186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(13194..13224)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13239..13260
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(13548..14136)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(14307..15167)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(15168..15272)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      15299..15754
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     15895..15911
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15981..16284
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        16285..16488
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        16533..16551
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatggtg agcaagggcg aggaggtcat caaagagttc
     3361 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     3421 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     3481 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     3541 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     3601 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     3661 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     3721 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     3781 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     3841 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     3901 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     3961 gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg caccggcagc
     4021 accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc cgtcatcaaa
     4081 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc
     4141 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     4201 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc
     4261 aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag
     4321 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag
     4381 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc
     4441 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc
     4501 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac
     4561 ggcggccgct acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg
     4621 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc
     4681 atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta cggcatggac
     4741 gagctgtaca agtaagctag acatgaaaaa aactaacact cctccggtac cgccaccatg
     4801 gtgcccaaga agaagaggaa agtctccaac ctgctgactg tgcaccaaaa cctgcctgcc
     4861 ctccctgtgg atgccacctc tgatgaagtc aggaagaacc tgatggacat gttcagggac
     4921 aggcaggcct tctctgaaca cacctggaag atgctcctgt ctgtgtgcag atcctgggct
     4981 gcctggtgca agctgaacaa caggaaatgg ttccctgctg aacctgagga tgtgagggac
     5041 tacctcctgt acctgcaagc cagaggcctg gctgtgaaga ccatccaaca gcacctgggc
     5101 cagctcaaca tgctgcacag gagatctggc ctgcctcgcc cttctgactc caatgctgtg
     5161 tccctggtga tgaggagaat cagaaaggag aatgtggatg ctggggagag agccaagcag
     5221 gccctggcct ttgaacgcac tgactttgac caagtcagat ccctgatgga gaactctgac
     5281 agatgccagg acatcaggaa cctggccttc ctgggcattg cctacaacac cctgctgcgc
     5341 attgccgaaa ttgccagaat cagagtgaag gacatctccc gcaccgatgg tgggagaatg
     5401 ctgatccaca ttggcaggac caagaccctg gtgtccacag ctggtgtgga gaaggccctg
     5461 tccctggggg ttaccaagct ggtggagaga tggatctctg tgtctggtgt ggctgatgac
     5521 cccaacaact acctgttctg ccgggtcaga aagaatggtg tggctgcccc ttctgccacc
     5581 tcccaactgt ccacccgggc cctggaaggg atctttgagg ccacccaccg cctgatctat
     5641 ggtgccaagg atgactctgg gcagagatac ctggcctggt ctggccactc tgccagagtg
     5701 ggtgctgcca gggacatggc cagggctggt gtgtccatcc ctgaaatcat gcaggctggt
     5761 ggctggacca atgtgaacat tgtgatgaac tacatcagaa acctggactc tgagactggg
     5821 gccatggtga ggctgctcga ggatggggac ggcagtggag gatccggatg agctagcggc
     5881 gcctagccgg tcatcctttt gacgattcca gtcccgagga taacctcctc tcgggattgg
     5941 ggggaatctt tggatccagt agtcctcctt gaactccgtc caacagggca gattcaagag
     6001 tcataagact ttcattaatc atttcagttg atcagacatg gtcgtgtaga ttctcttaat
     6061 acgggagatc ttctagcagt ttcagtgacc aacggtgctt tcattctcca ggaactgata
     6121 ccaaaggttg tggacaggcc aaggggtact tcggatgact ctgtgcttgg gcacagaaag
     6181 aggtcgtagt gtgccccccg atagcggact cgacatgaat caactaagaa aggcaatctg
     6241 cctcccatga tggacataag caatagttca caaccatctt gcatctcagt gaagtgtaca
     6301 taactattga gggctgggtc atctaagcat ttcagtcgag aaaaaaactg tagaccaaaa
     6361 gaacaactag caacacttct catccagaga cccatatcaa gatgctagat ccgggagagg
     6421 tttatgatga ccctattgat ccaattgagt cagaggctga acccagagga acccccactg
     6481 tccccaacat cttgaggaac tccgactaca atctcaattc tcctttgata gaggatcctg
     6541 ccaaactaat gttagaatgg ttgaagacag ggaacagacc ttatcggatg actttgacag
     6601 acaattgctc caggtcttac aaagttttga aagattattt caagaaagta gatttgggtt
     6661 ctctcaaagt gggcggaact gctgcacagt caatggtttc tctctggttg tgtggtgccc
     6721 actctgaatc aaacaggagc cggagatgta taaccgactt ggcccatttc tattccaagt
     6781 catcccccat agagaagcta ttgaattgta cgctaggaaa cagaggcctg agaatcccac
     6841 cagagggggt gttaaattgc ctcgagaggg tcaattatga caaggcattt gggaggtatc
     6901 tggccaacac gtattcctct tacttgtttt tccatgtaat caccttatac atgaatgcct
     6961 tagactggga agaggaaaag accatcctgg cattatggaa agatataacc tcagtggata
     7021 ccgagaagga cttggtcaaa ttcaaagatc aaatatgggg actgttgatt gtgacaaagg
     7081 actttgttta ctctcagagt tcaaactgtc tttttgacag aaactacaca ctgatgctaa
     7141 aggatctttt cttgtctcga ttcaactcct taatgattct gctttctccc cctgagcccc
     7201 gatactcaga tgacttaata tctcagctgt gccagctata catcgctggg gatcaagtct
     7261 tgtccatgtg tgggaactcc ggctatgaag tcatcaaaat attggagcca tatgtcgtga
     7321 acagtttggt ccagagggca gagaagttta ggcctctcat ccaccccttg ggagactttc
     7381 ctatgttcat aaaagacaag gtgaatcaac ttgaagggac ttttggtccc agtgcaaaga
     7441 ggttttttag ggttttagat caattcgaca acatacatga cttagtattt gtgtatggct
     7501 gttacagaca ttgggggcac ccctatatag attatcggaa gggtctgtcg aaactatatg
     7561 atcaagttca cattaagaaa gtaatagata agtcctacca ggagtgttta gcaagtgact
     7621 tggccagaag gatcctcaga tggggatttg acaagtactc caaatggtat ctagattcga
     7681 gattccttgc ccgagaccac cccttgactc cttatgtcaa gacccaaaca tggccaccca
     7741 aacatatagt agacttggtg ggggacacat ggcataagct cccgatcacg cagatctttg
     7801 aaattcctga atcaatggac ccgtcagaga tactggatga caaatcacat tctttcacca
     7861 gaacaaggtt agcttcttgg ctgtcagaga accgaggggg gcctgttcct agcgagaagg
     7921 tcattatcac ggccctgtct aagccacctg tcaatccccg agagtttttg aaatctatag
     7981 acctcggagg attgccagat gaggatttga taattggcct caaaccaaag gaacgggagt
     8041 tgaagatcga gggccgattc tttgctctaa tgtcatggaa tctaagatta tattttgtca
     8101 tcaccgaaaa gctcttggcc aactacattt tgccactttt tgacgcactg actatgacag
     8161 acaacctgaa caaggtgttc aaaaagttga tcgacagggt caccgggcaa gggcttttgg
     8221 actattctag ggtcacatac gcatttcacc tggactatga gaaatggaac aatcatcaaa
     8281 gattggagtc aacagaggat gtattctctg tcctagatca ggtgtttgga ttgaagaggg
     8341 tgttttctag aacacacgag ttttttcaga agtcctggat ctattattca gacagatcag
     8401 acctcattgg gttatgggag gaccaaatat attgcttgga tatgtctaac ggcccaacct
     8461 gctggaatgg ccaagatggc gggctagagg gcttgcggca gaagggctgg agtctagtca
     8521 gtttattaat gatagataga gaatctcaaa ccaggaacac aagaaccaag atactagctc
     8581 aaggagacaa ccaggttctg tgtcctacat acatgttgtc accgggattg tctcaagagg
     8641 ggcttctcta tgagttagag agcatatcaa ggaatgcact ctcaatatac cgagctatcg
     8701 aggaaggagc atctaagctg gggctgatca tcaagaagga agagaccatg tgtagttatg
     8761 actttctcat atatgggaag acccccttat ttcgaggcaa catattggtg cctgaatcca
     8821 aaagatgggc ccgagtctct tgcatctcta acgaccaaat agtcaacctc gccaatataa
     8881 tgtcgacagt atccaccaat gcgctgacag tggcacaaca ctctcaatct ctgatcaaac
     8941 ctatgaggga ttttctgctc atgtcagtac aggcagtttt ccactacctg ttgtttagcc
     9001 caatcctaaa aggcagagtt tataagattc tgagtgctga aggggagagc tttctcctag
     9061 ccatgtcgcg gataatctac ctagatcctt ctttgggagg ggtgtctgga atgtctctcg
     9121 ggaggttcca tatacgtcag ttctcagacc ctgtctctga agggttgtca ttctggagag
     9181 agatctggtt aggctctcat gagtcctgga ttcacgcgtt gtgtcaggag gccgggaacc
     9241 ccgatcttgg agagagaaca ctcgagagct tcactcgcct tttagaagat cctactacct
     9301 taaatatcaa aggaggggcc agccctacca ttctactcaa ggatgctatc agaaaggctc
     9361 tgtacgacga ggtggacaag gtggaaaatt cagagtttcg agaggcaatc ctgctgtcca
     9421 agacccatag agataacttt atactctttt taaaatctgt tgagcctctg ttccctcgat
     9481 ttctcagtga gctcttcagt tcgtctttct tgggaatacc ggagtcaatc attggactga
     9541 tacaaaactc ccggacaata agaaggcagt ttagaaagag tctctcaaga actttagaag
     9601 agtccttcta caactcagag atccacggga ttaatcggat aacccagaca cctcaaaggg
     9661 tcggaagggt gtggccttgc tcttcagaga gggcggatct acttagggag atctcttggg
     9721 ggaggaaagt ggtaggcacg acagttcctc acccttccga gatgttggag ttgtttccca
     9781 aatcctccat ttcctgcact tgtggagcaa cagggggagg caatcctaga gtctctgtat
     9841 cagtactccc gtccttcgat cagtcatttt tctcacgggg ccccctaaag ggatacttgg
     9901 gctcgtccac ctctatgtca acccagctat tccatgcatg ggaaaaagtc actaatgttc
     9961 atgtggtgaa aagggctcta tcgttaaaag aatctataaa ctggttcatc aataggaatt
    10021 ccaatttggc tcaaactcta attggaaaca tcatgtctct gacaggccct gacttccctc
    10081 tagaagaggc ccctgttttc aaacggacag ggtcagcctt gcataggttc aagtctgcca
    10141 gatacagcga aggagggtat tcttctgttt gccctaacct tctctcccat atctctgtta
    10201 gtacagacac tatgtctgat ttgacccaaa acgggaagaa ctatgatttc atgtttcagc
    10261 cattgatgct ttatgcgcaa acatggacat cggaactggt acagagggat acaagactta
    10321 gagactccac gtttcactgg caccttcggt gcaacagatg tgtgaggccc attgatgata
    10381 taacactgga aacttctcag atcttcgagt tcccggatgt gtcaaaaagg atatccagga
    10441 tggtttctgg agctgtccct caatttcaga agcttcctga tatccgtcta agaccaggtg
    10501 attttgaatc cctaagtggt agagaaaagt ctcgccatat agggtcagct caggggctct
    10561 tatactcaat cttagtagca attcacgact caggatacaa tgatgggacc atcttccctg
    10621 tcaacatata cggcaaagtt tcccccagag actatttgag agggcttgca agagggatct
    10681 tgatagggtc ctcgatttgc ttcttgacac gaatgacaaa tattaacatt aacagacctc
    10741 ttgaattgat ctcaggggta atttcctata ttctcctgag gctggataat catccctctc
    10801 tgtatataat gctcagagaa ccgtctctta gaggagaaat attctctatc cctcagaaaa
    10861 tccccgccgc ttacccaacc actatgagag aaggcaacag atcgattttg tgttacctcc
    10921 aacacgtgct acgctatgag cgagaggcaa tcacggcgtc cccggagaat gactggctgt
    10981 ggatcttctc agacttcaga agtgtgaaaa tgacgtactt gaccctcatt acttaccagt
    11041 ctcacctcct actccagagg gttgagagaa acttgtctaa gagtatgaga gctactctgc
    11101 gacaaatggg ttccttaatg aggcaagtgc tgggtgggca cggagaagat accttggagt
    11161 cagacgatga cattcaacga ttactaaaag actctttgcg aaagacaagg tgggtggatc
    11221 aagaggtgcg ccatgcagct agaaccatga atggagatta cagccccgac aagaaagtat
    11281 cccgtaaggt aggatgttca gaatgggtct gctctgctca acagattgcc gtctccacct
    11341 cagccaaccc ggcccctgtc tcagagcttg acattagggc cctctctaag cggtttcaaa
    11401 accccttgat ctcgggcctg agagtggttc agtgggcaac cggtgcccat tataagctta
    11461 agcctattct agatgatcta aatgttttcc catctctctg tcttgtaatt ggagacgggt
    11521 cagggggaat atcaagggca gttctcaaca tgtttccaga ttctaagctt gtgttcaaca
    11581 gcctattgga ggtgaatgat ctgatggctt ccggaacaca tccactgcct ccttcagcaa
    11641 tcatgagtgg aggagatgac atcatctcca gagtgataga ctttgactca atatgggaga
    11701 aaccgtccga cctgaggaac tcggccacct ggagatactt ccagtcggtt caaaaacagg
    11761 tcaacatgtc gtatgacctc attatttgtg atgcagaagt tactgatatt gcatctatca
    11821 accggataac tctgttgatg tctgatttcg cattgtctat agatggacca ctttatctgg
    11881 tcttcaaaac ttacgggact atgctagtaa acccggacta taaagctatt caacatctgt
    11941 caagagcgtt cccttcggtc acagggtttg taacccaagt aacttcatcc ttttcttctg
    12001 agctatacct ccggttctct aaacgaggaa agtttttcag ggacgccgag tacttgacct
    12061 cttccaccct tcgagagatg agccttgtgt tgttcaattg cagcagcccc aaaagtgaga
    12121 tgcagagagc tcgttcctta aactatcaag acctggtaag gggatttcct gaagagatca
    12181 tatcaaatcc ttacaacgaa atgatcataa ctctgattga caatgatgta gagtccttcc
    12241 tagtccacaa gatggtggat gatcttgagc tacagagggg aactctgtct aaagtggcta
    12301 tcattatatc catcatgatc gttttttcca atagagtctt caacatttcc aaacctttga
    12361 ctgacccctt gttctatcca ccgtctgatc ctaaaatcct gaggcacttc aacatatgtt
    12421 gcagtactat gatgtatcta tctaccgctt taggcgacgt ccctaacttc gcaagacttc
    12481 atgacctgta taacagacct ataacttgtt acttcagaaa acaagttatt cgagggaata
    12541 tttatctatc ttggagttgg tccgatgata ccccagtgtt caagagagta gcctgtaatt
    12601 ctagcttgag tctgtcatct cactggatca ggttgatcta caagatagtg aagactacca
    12661 gactcattgg cagcataaaa gacctatcag gagaggtaga acgacatctt catgggtata
    12721 acagatggat caccctcgag gatatccgat ctagatcatc cctactagat tacagttgtt
    12781 tgtaagccgg atattaccga aagcctgtgc atgctaaaat tcttgtatga tgcatcttga
    12841 aaaaaacaag atcttgaatc cggacctctg gttgtttgat tgttttttcc atctttattg
    12901 tttttttgtt aagcgtgggt cggcatggca tctccacctc ctcgcggtcc gacctgggca
    12961 tccgaaggag gacgcacgtc cactcggatg gctaagggag gggcggggat ccggctgcta
    13021 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
    13081 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaagtcgcg cttggcgtaa
    13141 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    13201 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    13261 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    13321 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    13381 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    13441 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    13501 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    13561 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    13621 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    13681 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    13741 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    13801 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    13861 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    13921 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    13981 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    14041 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    14101 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    14161 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    14221 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    14281 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    14341 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    14401 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    14461 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    14521 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    14581 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    14641 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    14701 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    14761 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    14821 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    14881 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    14941 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    15001 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    15061 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    15121 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    15181 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    15241 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    15301 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
    15361 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    15421 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    15481 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    15541 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    15601 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    15661 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
    15721 gcgaatttta acaaaatatt aacgcttaca atttccattc gccattcagg ctgcgcaact
    15781 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
    15841 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
    15901 cgacggccag tgagcgcgcc tagttattaa tagtaatcaa ttacggggtc attagttcat
    15961 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
    16021 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
    16081 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
    16141 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
    16201 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
    16261 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
    16321 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
    16381 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
    16441 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
    16501 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
    16561 gctggctaga ttaagcgtct gatgagtccg tgaggacgaa acccggcgta ccgggtc
//
