LOCUS       Exported               16819 bp DNA     circular SYN 07-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 7, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16819
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             71..1423
                     /codon_start=1
                     /label=N
                     /translation="MDADKIVFKVNNQVVSLKPEIIVDQYEYKYPAIKDLKKPCITLGK
                     APDLNKAYKSVLSGMNAAKLDPDDVCSYLAAAMQFFEGTCPEDWTSYGILIARKGDRIT
                     PNSLVEIKRTDVEGNWALTGGMELTRDPTVSEHASLVGLLLSLYRLSKISGQNTGNYKT
                     NIADRIEQIFETAPFVKIVEHHTLMTTHKMCANWSTIPNFRFLAGTYDMFFSRIEHLYS
                     AIRVGTVVTAYEDCSGLVSFTGFIKQINLTAREAILYFFHKNFEEEIRRMFEPGQETAV
                     PHSYFIHFRSLGLSGKSPYSSNAVGHVFNLIHFVGCYMGQVRSLNATVIAACAPHEMSV
                     LGGYLGEEFFGKGTFERRFFRDEKELQEYEAAELTKSDVALADDGTVNSDDEDYFSGET
                     RSPEAVYTRIMMNGGRLKRSHIRRYVSVSSNHQARPNSFAEFLNKTYSNDS"
     CDS             1514..2407
                     /codon_start=1
                     /label=P
                     /translation="MSKIFVNPSAIRAGLADLEMAEETVDLINRNIEDNQAHLQGEPIE
                     VDNLPEDMKRLHLDDEKSSNLGEMVRVGEGKYREDFQMDEGEDPNLLFQSYLDNVGVQI
                     VRQMRSGERFLKIWSQTVEEIVSYVTVNFPNPPRRSSEDKSTQTTGRELKKETTSAFSQ
                     RESQPSKARMVAQVAPGPPALEWSATNEEDDLSVEAEIAHQIAESFSKKYKFPSRSSGI
                     FLYNFEQLKMNLDDIVKEAKNVPGVTRLAHDGSKIPLRCVLGWVALANSKKFQLLVEAD
                     KLSKIMQDDLNRYTSC"
     CDS             2496..3104
                     /codon_start=1
                     /label=M
                     /translation="MNVLRKIVKKCRDEDTQKPSPVSAPPYDDDLWLPPPEYVPLKELT
                     SKKNMRNFCVNGEVKACSPNGYSFRILRHILRSFNEIYSGNHRMIGLVKIVVGLALSGA
                     PVPEGMNWVYKLRRTLIFQWADSRGPLEGEELDYSQEITWDDDTEFVGLQIRVSARQCH
                     IQGRIWCINTNSRACQLWSDMSLQTQRSEEDKDSSLLLE"
     CDS             3325..4755
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             4802..6073
                     /codon_start=1
                     /label=FlpO
                     /translation="MSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     CDS             6604..12987
                     /codon_start=1
                     /label=L
                     /translation="MLDPGEVYDDPIDPIESEAEPRGTPTVPNILRNSDYNLNSPLIED
                     PAKLMLEWLKTGNRPYRMTLTDNCSRSYKVLKDYFKKVDLGSLKVGGTAAQSMVSLWLC
                     GAHSESNRSRRCITDLAHFYSKSSPIEKLLNCTLGNRGLRIPPEGVLNCLERVNYDKAF
                     GRYLANTYSSYLFFHVITLYMNALDWEEEKTILALWKDITSVDTEKDLVKFKDQIWGLL
                     IVTKDFVYSQSSNCLFDRNYTLMLKDLFLSRFNSLMILLSPPEPRYSDDLISQLCQLYI
                     AGDQVLSMCGNSGYEVIKILEPYVVNSLVQRAEKFRPLIHPLGDFPMFIKDKVNQLEGT
                     FGPSAKRFFRVLDQFDNIHDLVFVYGCYRHWGHPYIDYRKGLSKLYDQVHIKKVIDKSY
                     QECLASDLARRILRWGFDKYSKWYLDSRFLARDHPLTPYVKTQTWPPKHIVDLVGDTWH
                     KLPITQIFEIPESMDPSEILDDKSHSFTRTRLASWLSENRGGPVPSEKVIITALSKPPV
                     NPREFLKSIDLGGLPDEDLIIGLKPKERELKIEGRFFALMSWNLRLYFVITEKLLANYI
                     LPLFDALTMTDNLNKVFKKLIDRVTGQGLLDYSRVTYAFHLDYEKWNNHQRLESTEDVF
                     SVLDQVFGLKRVFSRTHEFFQKSWIYYSDRSDLIGLWEDQIYCLDMSNGPTCWNGQDGG
                     LEGLRQKGWSLVSLLMIDRESQTRNTRTKILAQGDNQVLCPTYMLSPGLSQEGLLYELE
                     SISRNALSIYRAIEEGASKLGLIIKKEETMCSYDFLIYGKTPLFRGNILVPESKRWARV
                     SCISNDQIVNLANIMSTVSTNALTVAQHSQSLIKPMRDFLLMSVQAVFHYLLFSPILKG
                     RVYKILSAEGESFLLAMSRIIYLDPSLGGVSGMSLGRFHIRQFSDPVSEGLSFWREIWL
                     GSHESWIHALCQEAGNPDLGERTLESFTRLLEDPTTLNIKGGASPTILLKDAIRKALYD
                     EVDKVENSEFREAILLSKTHRDNFILFLKSVEPLFPRFLSELFSSSFLGIPESIIGLIQ
                     NSRTIRRQFRKSLSRTLEESFYNSEIHGINRITQTPQRVGRVWPCSSERADLLREISWG
                     RKVVGTTVPHPSEMLELFPKSSISCTCGATGGGNPRVSVSVLPSFDQSFFSRGPLKGYL
                     GSSTSMSTQLFHAWEKVTNVHVVKRALSLKESINWFINRNSNLAQTLIGNIMSLTGPDF
                     PLEEAPVFKRTGSALHRFKSARYSEGGYSSVCPNLLSHISVSTDTMSDLTQNGKNYDFM
                     FQPLMLYAQTWTSELVQRDTRLRDSTFHWHLRCNRCVRPIDDITLETSQIFEFPDVSKR
                     ISRMVSGAVPQFQKLPDIRLRPGDFESLSGREKSRHIGSAQGLLYSILVAIHDSGYNDG
                     TIFPVNIYGKVSPRDYLRGLARGILIGSSICFLTRMTNININRPLELISGVISYILLRL
                     DNHPSLYIMLREPSLRGEIFSIPQKIPAAYPTTMREGNRSILCYLQHVLRYEREAITAS
                     PENDWLWIFSDFRSVKMTYLTLITYQSHLLLQRVERNLSKSMRATLRQMGSLMRQVLGG
                     HGEDTLESDDDIQRLLKDSLRKTRWVDQEVRHAARTMNGDYSPDKKVSRKVGCSEWVCS
                     AQQIAVSTSANPAPVSELDIRALSKRFQNPLISGLRVVQWATGAHYKLKPILDDLNVFP
                     SLCLVIGDGSGGISRAVLNMFPDSKLVFNSLLEVNDLMASGTHPLPPSAIMSGGDDIIS
                     RVIDFDSIWEKPSDLRNSATWRYFQSVQKQVNMSYDLIICDAEVTDIASINRITLLMSD
                     FALSIDGPLYLVFKTYGTMLVNPDYKAIQHLSRAFPSVTGFVTQVTSSFSSELYLRFSK
                     RGKFFRDAEYLTSSTLREMSLVLFNCSSPKSEMQRARSLNYQDLVRGFPEEIISNPYNE
                     MIITLIDNDVESFLVHKMVDDLELQRGTLSKVAIIISIMIVFSNRVFNISKPLTDPLFY
                     PPSDPKILRHFNICCSTMMYLSTALGDVPNFARLHDLYNRPITCYFRKQVIRGNIYLSW
                     SWSDDTPVFKRVACNSSLSLSSHWIRLIYKIVKTTRLIGSIKDLSGEVERHLHGYNRWI
                     TLEDIRSRSSLLDYSCL"
     CDS             8248..8259
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(10051..10062)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      13273..13320
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(13348..13364)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    13372..13388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(13396..13426)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13441..13462
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(13750..14338)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(14509..15369)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(15370..15474)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      15501..15956
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     16097..16113
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        16183..16486
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        16487..16690
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        16735..16753
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatggtg agcaagggcg aggaggtcat caaagagttc
     3361 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     3421 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     3481 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     3541 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     3601 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     3661 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     3721 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     3781 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     3841 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     3901 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     3961 gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg caccggcagc
     4021 accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc cgtcatcaaa
     4081 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc
     4141 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     4201 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc
     4261 aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag
     4321 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag
     4381 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc
     4441 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc
     4501 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac
     4561 ggcggccgct acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg
     4621 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc
     4681 atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta cggcatggac
     4741 gagctgtaca agtaagctag acatgaaaaa aactaacact cctccggtac cggatcccac
     4801 catgagccag ttcgacatcc tgtgcaagac cccccccaag gtgctggtgc ggcagttcgt
     4861 ggagagattc gagaggccca gcggcgagaa gatcgccagc tgtgccgccg agctgaccta
     4921 cctgtgctgg atgatcaccc acaacggcac cgccatcaag agggccacct tcatgagcta
     4981 caacaccatc atcagcaaca gcctgagctt cgacatcgtg aacaagagcc tgcagttcaa
     5041 gtacaagacc cagaaggcca ccatcctgga ggccagcctg aagaagctga tccccgcctg
     5101 ggagttcacc atcatccctt acaacggcca gaagcaccag agcgacatca ccgacatcgt
     5161 gtccagcctg cagctgcagt tcgagagcag cgaggaggcc gacaagggca acagccacag
     5221 caagaagatg ctgaaggccc tgctgtccga gggcgagagc atctgggaga tcaccgagaa
     5281 gatcctgaac agcttcgagt acaccagcag gttcaccaag accaagaccc tgtaccagtt
     5341 cctgttcctg gccacattca tcaactgcgg caggttcagc gacatcaaga acgtggaccc
     5401 caagagcttc aagctggtgc agaacaagta cctgggcgtg atcattcagt gcctggtgac
     5461 cgagaccaag acaagcgtgt ccaggcacat ctactttttc agcgccagag gcaggatcga
     5521 ccccctggtg tacctggacg agttcctgag gaacagcgag cccgtgctga agagagtgaa
     5581 caggaccggc aacagcagca gcaacaagca ggagtaccag ctgctgaagg acaacctggt
     5641 gcgcagctac aacaaggccc tgaagaagaa cgccccctac cccatcttcg ctatcaagaa
     5701 cggccctaag agccacatcg gcaggcacct gatgaccagc tttctgagca tgaagggcct
     5761 gaccgagctg acaaacgtgg tgggcaactg gagcgacaag agggcctccg ccgtggccag
     5821 gaccacctac acccaccaga tcaccgccat ccccgaccac tacttcgccc tggtgtccag
     5881 gtactacgcc tacgacccca tcagcaagga gatgatcgcc ctgaaggacg agaccaaccc
     5941 catcgaggag tggcagcaca tcgagcagct gaagggcagc gccgagggca gcatcagata
     6001 ccccgcctgg aacggcatca tcagccagga ggtgctggac tacctgagca gctacatcaa
     6061 caggcggatc tgagctagcg gcgcctagcc ggtcatcctt ttgacgattc cagtcccgag
     6121 gataacctcc tctcgggatt ggggggaatc tttggatcca gtagtcctcc ttgaactccg
     6181 tccaacaggg cagattcaag agtcataaga ctttcattaa tcatttcagt tgatcagaca
     6241 tggtcgtgta gattctctta atacgggaga tcttctagca gtttcagtga ccaacggtgc
     6301 tttcattctc caggaactga taccaaaggt tgtggacagg ccaaggggta cttcggatga
     6361 ctctgtgctt gggcacagaa agaggtcgta gtgtgccccc cgatagcgga ctcgacatga
     6421 atcaactaag aaaggcaatc tgcctcccat gatggacata agcaatagtt cacaaccatc
     6481 ttgcatctca gtgaagtgta cataactatt gagggctggg tcatctaagc atttcagtcg
     6541 agaaaaaaac tgtagaccaa aagaacaact agcaacactt ctcatccaga gacccatatc
     6601 aagatgctag atccgggaga ggtttatgat gaccctattg atccaattga gtcagaggct
     6661 gaacccagag gaacccccac tgtccccaac atcttgagga actccgacta caatctcaat
     6721 tctcctttga tagaggatcc tgccaaacta atgttagaat ggttgaagac agggaacaga
     6781 ccttatcgga tgactttgac agacaattgc tccaggtctt acaaagtttt gaaagattat
     6841 ttcaagaaag tagatttggg ttctctcaaa gtgggcggaa ctgctgcaca gtcaatggtt
     6901 tctctctggt tgtgtggtgc ccactctgaa tcaaacagga gccggagatg tataaccgac
     6961 ttggcccatt tctattccaa gtcatccccc atagagaagc tattgaattg tacgctagga
     7021 aacagaggcc tgagaatccc accagagggg gtgttaaatt gcctcgagag ggtcaattat
     7081 gacaaggcat ttgggaggta tctggccaac acgtattcct cttacttgtt tttccatgta
     7141 atcaccttat acatgaatgc cttagactgg gaagaggaaa agaccatcct ggcattatgg
     7201 aaagatataa cctcagtgga taccgagaag gacttggtca aattcaaaga tcaaatatgg
     7261 ggactgttga ttgtgacaaa ggactttgtt tactctcaga gttcaaactg tctttttgac
     7321 agaaactaca cactgatgct aaaggatctt ttcttgtctc gattcaactc cttaatgatt
     7381 ctgctttctc cccctgagcc ccgatactca gatgacttaa tatctcagct gtgccagcta
     7441 tacatcgctg gggatcaagt cttgtccatg tgtgggaact ccggctatga agtcatcaaa
     7501 atattggagc catatgtcgt gaacagtttg gtccagaggg cagagaagtt taggcctctc
     7561 atccacccct tgggagactt tcctatgttc ataaaagaca aggtgaatca acttgaaggg
     7621 acttttggtc ccagtgcaaa gaggtttttt agggttttag atcaattcga caacatacat
     7681 gacttagtat ttgtgtatgg ctgttacaga cattgggggc acccctatat agattatcgg
     7741 aagggtctgt cgaaactata tgatcaagtt cacattaaga aagtaataga taagtcctac
     7801 caggagtgtt tagcaagtga cttggccaga aggatcctca gatggggatt tgacaagtac
     7861 tccaaatggt atctagattc gagattcctt gcccgagacc accccttgac tccttatgtc
     7921 aagacccaaa catggccacc caaacatata gtagacttgg tgggggacac atggcataag
     7981 ctcccgatca cgcagatctt tgaaattcct gaatcaatgg acccgtcaga gatactggat
     8041 gacaaatcac attctttcac cagaacaagg ttagcttctt ggctgtcaga gaaccgaggg
     8101 gggcctgttc ctagcgagaa ggtcattatc acggccctgt ctaagccacc tgtcaatccc
     8161 cgagagtttt tgaaatctat agacctcgga ggattgccag atgaggattt gataattggc
     8221 ctcaaaccaa aggaacggga gttgaagatc gagggccgat tctttgctct aatgtcatgg
     8281 aatctaagat tatattttgt catcaccgaa aagctcttgg ccaactacat tttgccactt
     8341 tttgacgcac tgactatgac agacaacctg aacaaggtgt tcaaaaagtt gatcgacagg
     8401 gtcaccgggc aagggctttt ggactattct agggtcacat acgcatttca cctggactat
     8461 gagaaatgga acaatcatca aagattggag tcaacagagg atgtattctc tgtcctagat
     8521 caggtgtttg gattgaagag ggtgttttct agaacacacg agttttttca gaagtcctgg
     8581 atctattatt cagacagatc agacctcatt gggttatggg aggaccaaat atattgcttg
     8641 gatatgtcta acggcccaac ctgctggaat ggccaagatg gcgggctaga gggcttgcgg
     8701 cagaagggct ggagtctagt cagtttatta atgatagata gagaatctca aaccaggaac
     8761 acaagaacca agatactagc tcaaggagac aaccaggttc tgtgtcctac atacatgttg
     8821 tcaccgggat tgtctcaaga ggggcttctc tatgagttag agagcatatc aaggaatgca
     8881 ctctcaatat accgagctat cgaggaagga gcatctaagc tggggctgat catcaagaag
     8941 gaagagacca tgtgtagtta tgactttctc atatatggga agaccccctt atttcgaggc
     9001 aacatattgg tgcctgaatc caaaagatgg gcccgagtct cttgcatctc taacgaccaa
     9061 atagtcaacc tcgccaatat aatgtcgaca gtatccacca atgcgctgac agtggcacaa
     9121 cactctcaat ctctgatcaa acctatgagg gattttctgc tcatgtcagt acaggcagtt
     9181 ttccactacc tgttgtttag cccaatccta aaaggcagag tttataagat tctgagtgct
     9241 gaaggggaga gctttctcct agccatgtcg cggataatct acctagatcc ttctttggga
     9301 ggggtgtctg gaatgtctct cgggaggttc catatacgtc agttctcaga ccctgtctct
     9361 gaagggttgt cattctggag agagatctgg ttaggctctc atgagtcctg gattcacgcg
     9421 ttgtgtcagg aggccgggaa ccccgatctt ggagagagaa cactcgagag cttcactcgc
     9481 cttttagaag atcctactac cttaaatatc aaaggagggg ccagccctac cattctactc
     9541 aaggatgcta tcagaaaggc tctgtacgac gaggtggaca aggtggaaaa ttcagagttt
     9601 cgagaggcaa tcctgctgtc caagacccat agagataact ttatactctt tttaaaatct
     9661 gttgagcctc tgttccctcg atttctcagt gagctcttca gttcgtcttt cttgggaata
     9721 ccggagtcaa tcattggact gatacaaaac tcccggacaa taagaaggca gtttagaaag
     9781 agtctctcaa gaactttaga agagtccttc tacaactcag agatccacgg gattaatcgg
     9841 ataacccaga cacctcaaag ggtcggaagg gtgtggcctt gctcttcaga gagggcggat
     9901 ctacttaggg agatctcttg ggggaggaaa gtggtaggca cgacagttcc tcacccttcc
     9961 gagatgttgg agttgtttcc caaatcctcc atttcctgca cttgtggagc aacaggggga
    10021 ggcaatccta gagtctctgt atcagtactc ccgtccttcg atcagtcatt tttctcacgg
    10081 ggccccctaa agggatactt gggctcgtcc acctctatgt caacccagct attccatgca
    10141 tgggaaaaag tcactaatgt tcatgtggtg aaaagggctc tatcgttaaa agaatctata
    10201 aactggttca tcaataggaa ttccaatttg gctcaaactc taattggaaa catcatgtct
    10261 ctgacaggcc ctgacttccc tctagaagag gcccctgttt tcaaacggac agggtcagcc
    10321 ttgcataggt tcaagtctgc cagatacagc gaaggagggt attcttctgt ttgccctaac
    10381 cttctctccc atatctctgt tagtacagac actatgtctg atttgaccca aaacgggaag
    10441 aactatgatt tcatgtttca gccattgatg ctttatgcgc aaacatggac atcggaactg
    10501 gtacagaggg atacaagact tagagactcc acgtttcact ggcaccttcg gtgcaacaga
    10561 tgtgtgaggc ccattgatga tataacactg gaaacttctc agatcttcga gttcccggat
    10621 gtgtcaaaaa ggatatccag gatggtttct ggagctgtcc ctcaatttca gaagcttcct
    10681 gatatccgtc taagaccagg tgattttgaa tccctaagtg gtagagaaaa gtctcgccat
    10741 atagggtcag ctcaggggct cttatactca atcttagtag caattcacga ctcaggatac
    10801 aatgatggga ccatcttccc tgtcaacata tacggcaaag tttcccccag agactatttg
    10861 agagggcttg caagagggat cttgataggg tcctcgattt gcttcttgac acgaatgaca
    10921 aatattaaca ttaacagacc tcttgaattg atctcagggg taatttccta tattctcctg
    10981 aggctggata atcatccctc tctgtatata atgctcagag aaccgtctct tagaggagaa
    11041 atattctcta tccctcagaa aatccccgcc gcttacccaa ccactatgag agaaggcaac
    11101 agatcgattt tgtgttacct ccaacacgtg ctacgctatg agcgagaggc aatcacggcg
    11161 tccccggaga atgactggct gtggatcttc tcagacttca gaagtgtgaa aatgacgtac
    11221 ttgaccctca ttacttacca gtctcacctc ctactccaga gggttgagag aaacttgtct
    11281 aagagtatga gagctactct gcgacaaatg ggttccttaa tgaggcaagt gctgggtggg
    11341 cacggagaag ataccttgga gtcagacgat gacattcaac gattactaaa agactctttg
    11401 cgaaagacaa ggtgggtgga tcaagaggtg cgccatgcag ctagaaccat gaatggagat
    11461 tacagccccg acaagaaagt atcccgtaag gtaggatgtt cagaatgggt ctgctctgct
    11521 caacagattg ccgtctccac ctcagccaac ccggcccctg tctcagagct tgacattagg
    11581 gccctctcta agcggtttca aaaccccttg atctcgggcc tgagagtggt tcagtgggca
    11641 accggtgccc attataagct taagcctatt ctagatgatc taaatgtttt cccatctctc
    11701 tgtcttgtaa ttggagacgg gtcaggggga atatcaaggg cagttctcaa catgtttcca
    11761 gattctaagc ttgtgttcaa cagcctattg gaggtgaatg atctgatggc ttccggaaca
    11821 catccactgc ctccttcagc aatcatgagt ggaggagatg acatcatctc cagagtgata
    11881 gactttgact caatatggga gaaaccgtcc gacctgagga actcggccac ctggagatac
    11941 ttccagtcgg ttcaaaaaca ggtcaacatg tcgtatgacc tcattatttg tgatgcagaa
    12001 gttactgata ttgcatctat caaccggata actctgttga tgtctgattt cgcattgtct
    12061 atagatggac cactttatct ggtcttcaaa acttacggga ctatgctagt aaacccggac
    12121 tataaagcta ttcaacatct gtcaagagcg ttcccttcgg tcacagggtt tgtaacccaa
    12181 gtaacttcat ccttttcttc tgagctatac ctccggttct ctaaacgagg aaagtttttc
    12241 agggacgccg agtacttgac ctcttccacc cttcgagaga tgagccttgt gttgttcaat
    12301 tgcagcagcc ccaaaagtga gatgcagaga gctcgttcct taaactatca agacctggta
    12361 aggggatttc ctgaagagat catatcaaat ccttacaacg aaatgatcat aactctgatt
    12421 gacaatgatg tagagtcctt cctagtccac aagatggtgg atgatcttga gctacagagg
    12481 ggaactctgt ctaaagtggc tatcattata tccatcatga tcgttttttc caatagagtc
    12541 ttcaacattt ccaaaccttt gactgacccc ttgttctatc caccgtctga tcctaaaatc
    12601 ctgaggcact tcaacatatg ttgcagtact atgatgtatc tatctaccgc tttaggcgac
    12661 gtccctaact tcgcaagact tcatgacctg tataacagac ctataacttg ttacttcaga
    12721 aaacaagtta ttcgagggaa tatttatcta tcttggagtt ggtccgatga taccccagtg
    12781 ttcaagagag tagcctgtaa ttctagcttg agtctgtcat ctcactggat caggttgatc
    12841 tacaagatag tgaagactac cagactcatt ggcagcataa aagacctatc aggagaggta
    12901 gaacgacatc ttcatgggta taacagatgg atcaccctcg aggatatccg atctagatca
    12961 tccctactag attacagttg tttgtaagcc ggatattacc gaaagcctgt gcatgctaaa
    13021 attcttgtat gatgcatctt gaaaaaaaca agatcttgaa tccggacctc tggttgtttg
    13081 attgtttttt ccatctttat tgtttttttg ttaagcgtgg gtcggcatgg catctccacc
    13141 tcctcgcggt ccgacctggg catccgaagg aggacgcacg tccactcgga tggctaaggg
    13201 aggggcgggg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
    13261 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
    13321 ctgaaagtcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    13381 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    13441 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    13501 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    13561 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    13621 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    13681 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    13741 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    13801 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    13861 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    13921 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    13981 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    14041 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    14101 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    14161 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    14221 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    14281 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    14341 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    14401 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    14461 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    14521 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    14581 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    14641 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    14701 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    14761 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    14821 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    14881 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    14941 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    15001 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    15061 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    15121 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    15181 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    15241 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    15301 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    15361 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    15421 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    15481 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
    15541 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
    15601 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
    15661 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
    15721 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    15781 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    15841 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
    15901 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttccat
    15961 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    16021 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    16081 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cctagttatt aatagtaatc
    16141 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
    16201 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
    16261 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
    16321 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
    16381 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
    16441 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
    16501 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
    16561 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
    16621 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
    16681 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
    16741 gactcactat agggagaccc aagctggcta gattaagcgt ctgatgagtc cgtgaggacg
    16801 aaacccggcg taccgggtc
//
