LOCUS       Exported               16313 bp DNA     circular SYN 23-DEC-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 16313)
  AUTHORS   Yoav Ben Simon
  TITLE     Direct Submission
  JOURNAL   Exported Jun 23, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16313
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     CDS             71..1423
                     /codon_start=1
                     /label=N
                     /translation="MDADKIVFKVNNQVVSLKPEIIVDQYEYKYPAIKDLKKPCITLGK
                     APDLNKAYKSVLSGMNAAKLDPDDVCSYLAAAMQFFEGTCPEDWTSYGILIARKGDRIT
                     PNSLVEIKRTDVEGNWALTGGMELTRDPTVSEHASLVGLLLSLYRLSKISGQNTGNYKT
                     NIADRIEQIFETAPFVKIVEHHTLMTTHKMCANWSTIPNFRFLAGTYDMFFSRIEHLYS
                     AIRVGTVVTAYEDCSGLVSFTGFIKQINLTAREAILYFFHKNFEEEIRRMFEPGQETAV
                     PHSYFIHFRSLGLSGKSPYSSNAVGHVFNLIHFVGCYMGQVRSLNATVIAACAPHEMSV
                     LGGYLGEEFFGKGTFERRFFRDEKELQEYEAAELTKSDVALADDGTVNSDDEDYFSGET
                     RSPEAVYTRIMMNGGRLKRSHIRRYVSVSSNHQARPNSFAEFLNKTYSNDS"
     CDS             1514..2407
                     /codon_start=1
                     /label=P
                     /translation="MSKIFVNPSAIRAGLADLEMAEETVDLINRNIEDNQAHLQGEPIE
                     VDNLPEDMKRLHLDDEKSSNLGEMVRVGEGKYREDFQMDEGEDPNLLFQSYLDNVGVQI
                     VRQMRSGERFLKIWSQTVEEIVSYVTVNFPNPPRRSSEDKSTQTTGRELKKETTSAFSQ
                     RESQPSKARMVAQVAPGPPALEWSATNEEDDLSVEAEIAHQIAESFSKKYKFPSRSSGI
                     FLYNFEQLKMNLDDIVKEAKNVPGVTRLAHDGSKIPLRCVLGWVALANSKKFQLLVEAD
                     KLSKIMQDDLNRYTSC"
     CDS             2496..3104
                     /codon_start=1
                     /label=M
                     /translation="MNVLRKIVKKCRDEDTQKPSPVSAPPYDDDLWLPPPEYVPLKELT
                     SKKNMRNFCVNGEVKACSPNGYSFRILRHILRSFNEIYSGNHRMIGLVKIVVGLALSGA
                     PVPEGMNWVYKLRRTLIFQWADSRGPLEGEELDYSQEITWDDDTEFVGLQIRVSARQCH
                     IQGRIWCINTNSRACQLWSDMSLQTQRSEEDKDSSLLLE"
     CDS             3325..4032
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             4048..4068
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4072..4092
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4096..4116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     4198..4230
                     /label=Fragment.FOR
     regulatory      4214..4223
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4220..5572
                     /codon_start=1
                     /label=GCaMP7f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKELFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     CDS             4229..4246
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4250..4282
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             4286..4309
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      4316..4325
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4337..4393
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     4606..4627
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4733..4754)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4994..5013)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(5542..5587)
                     /label=Fragment.REV
     CDS             6098..12481
                     /codon_start=1
                     /label=L
                     /translation="MLDPGEVYDDPIDPIESEAEPRGTPTVPNILRNSDYNLNSPLIED
                     PAKLMLEWLKTGNRPYRMTLTDNCSRSYKVLKDYFKKVDLGSLKVGGTAAQSMVSLWLC
                     GAHSESNRSRRCITDLAHFYSKSSPIEKLLNCTLGNRGLRIPPEGVLNCLERVNYDKAF
                     GRYLANTYSSYLFFHVITLYMNALDWEEEKTILALWKDITSVDTEKDLVKFKDQIWGLL
                     IVTKDFVYSQSSNCLFDRNYTLMLKDLFLSRFNSLMILLSPPEPRYSDDLISQLCQLYI
                     AGDQVLSMCGNSGYEVIKILEPYVVNSLVQRAEKFRPLIHPLGDFPMFIKDKVNQLEGT
                     FGPSAKRFFRVLDQFDNIHDLVFVYGCYRHWGHPYIDYRKGLSKLYDQVHIKKVIDKSY
                     QECLASDLARRILRWGFDKYSKWYLDSRFLARDHPLTPYVKTQTWPPKHIVDLVGDTWH
                     KLPITQIFEIPESMDPSEILDDKSHSFTRTRLASWLSENRGGPVPSEKVIITALSKPPV
                     NPREFLKSIDLGGLPDEDLIIGLKPKERELKIEGRFFALMSWNLRLYFVITEKLLANYI
                     LPLFDALTMTDNLNKVFKKLIDRVTGQGLLDYSRVTYAFHLDYEKWNNHQRLESTEDVF
                     SVLDQVFGLKRVFSRTHEFFQKSWIYYSDRSDLIGLWEDQIYCLDMSNGPTCWNGQDGG
                     LEGLRQKGWSLVSLLMIDRESQTRNTRTKILAQGDNQVLCPTYMLSPGLSQEGLLYELE
                     SISRNALSIYRAIEEGASKLGLIIKKEETMCSYDFLIYGKTPLFRGNILVPESKRWARV
                     SCISNDQIVNLANIMSTVSTNALTVAQHSQSLIKPMRDFLLMSVQAVFHYLLFSPILKG
                     RVYKILSAEGESFLLAMSRIIYLDPSLGGVSGMSLGRFHIRQFSDPVSEGLSFWREIWL
                     GSHESWIHALCQEAGNPDLGERTLESFTRLLEDPTTLNIKGGASPTILLKDAIRKALYD
                     EVDKVENSEFREAILLSKTHRDNFILFLKSVEPLFPRFLSELFSSSFLGIPESIIGLIQ
                     NSRTIRRQFRKSLSRTLEESFYNSEIHGINRITQTPQRVGRVWPCSSERADLLREISWG
                     RKVVGTTVPHPSEMLELFPKSSISCTCGATGGGNPRVSVSVLPSFDQSFFSRGPLKGYL
                     GSSTSMSTQLFHAWEKVTNVHVVKRALSLKESINWFINRNSNLAQTLIGNIMSLTGPDF
                     PLEEAPVFKRTGSALHRFKSARYSEGGYSSVCPNLLSHISVSTDTMSDLTQNGKNYDFM
                     FQPLMLYAQTWTSELVQRDTRLRDSTFHWHLRCNRCVRPIDDITLETSQIFEFPDVSKR
                     ISRMVSGAVPQFQKLPDIRLRPGDFESLSGREKSRHIGSAQGLLYSILVAIHDSGYNDG
                     TIFPVNIYGKVSPRDYLRGLARGILIGSSICFLTRMTNININRPLELISGVISYILLRL
                     DNHPSLYIMLREPSLRGEIFSIPQKIPAAYPTTMREGNRSILCYLQHVLRYEREAITAS
                     PENDWLWIFSDFRSVKMTYLTLITYQSHLLLQRVERNLSKSMRATLRQMGSLMRQVLGG
                     HGEDTLESDDDIQRLLKDSLRKTRWVDQEVRHAARTMNGDYSPDKKVSRKVGCSEWVCS
                     AQQIAVSTSANPAPVSELDIRALSKRFQNPLISGLRVVQWATGAHYKLKPILDDLNVFP
                     SLCLVIGDGSGGISRAVLNMFPDSKLVFNSLLEVNDLMASGTHPLPPSAIMSGGDDIIS
                     RVIDFDSIWEKPSDLRNSATWRYFQSVQKQVNMSYDLIICDAEVTDIASINRITLLMSD
                     FALSIDGPLYLVFKTYGTMLVNPDYKAIQHLSRAFPSVTGFVTQVTSSFSSELYLRFSK
                     RGKFFRDAEYLTSSTLREMSLVLFNCSSPKSEMQRARSLNYQDLVRGFPEEIISNPYNE
                     MIITLIDNDVESFLVHKMVDDLELQRGTLSKVAIIISIMIVFSNRVFNISKPLTDPLFY
                     PPSDPKILRHFNICCSTMMYLSTALGDVPNFARLHDLYNRPITCYFRKQVIRGNIYLSW
                     SWSDDTPVFKRVACNSSLSLSSHWIRLIYKIVKTTRLIGSIKDLSGEVERHLHGYNRWI
                     TLEDIRSRSSLLDYSCL"
     CDS             7742..7753
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(9545..9556)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      12767..12814
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12842..12858)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12866..12882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12890..12920)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12935..12956
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(13244..13832)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(14003..14863)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(14864..14968)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14995..15450
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     15591..15607
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15677..15980
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15981..16184
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        16229..16247
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccggtaaagc caccatggtg agcaagggcg aggaggataa catggccatc
     3361 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     3421 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     3481 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     3541 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     3601 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     3661 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     3721 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     3781 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     3841 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     3901 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     3961 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     4021 gagctgtaca agagatctcg agctgatcca aaaaagaaga gaaaggtaga tccaaaaaag
     4081 aagagaaagg tagatccaaa aaagaagaga aaggtaggat ccagataact gatcataatc
     4141 agccatacca catttggaat tcgtcgaggg ggcgcgccgc tagacatgaa aaaaactaac
     4201 actcctccgg tacgccacca tgggttctca tcatcatcat catcatggta tggctagcat
     4261 gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg atctcgccac
     4321 catggtcgac tcatcacgtc gtaagtggaa taagacaggt cacgcagtca gagctatagg
     4381 tcggctgagc tcactcgaga acgtctatat caaggccgac aagcagaaga acggcatcaa
     4441 ggcgaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg cctaccacta
     4501 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     4561 cgtgcagtcc aaactttcga aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     4621 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg
     4681 agggagcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     4741 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc
     4801 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     4861 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     4921 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacatcc aggagcgcac
     4981 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     5041 caccctggtg aaccgcatcg agcttaaggg catcgacttc aaggaggacg gcaacatcct
     5101 ggggcacaag ctggagtaca acctgccgga ccaactgact gaagagcaga tcgcagaatt
     5161 taaagagctg ttctccctat ttgacaagga cggggatggg acaataacaa ccaaggagct
     5221 ggggacggtg atgcggtctc tggggcagaa ccccacagaa gcagagctgc aggacatgat
     5281 caatgaagta gatgccgacg gtgacggcac aatcgacttc cctgagttcc tgacaatgat
     5341 ggcaagaaaa atgaaataca cagacagtga agaagaaatt agagaagcgt tccgtgtgtt
     5401 tgataaggat ggcaatggct acatcagtgc agcagagctt cgccacgtga tgacaaacct
     5461 tggagagaag ttaacagatg aagaggttga tgaaatgatc agggaagcag acatcgatgg
     5521 ggatggtcag gtaaactacg aagagtttgt acaaatgatg acagcgaagt gacggcgcct
     5581 agccggtcat ccttttgacg attccagtcc cgaggataac ctcctctcgg gattgggggg
     5641 aatctttgga tccagtagtc ctccttgaac tccgtccaac agggcagatt caagagtcat
     5701 aagactttca ttaatcattt cagttgatca gacatggtcg tgtagattct cttaatacgg
     5761 gagatcttct agcagtttca gtgaccaacg gtgctttcat tctccaggaa ctgataccaa
     5821 aggttgtgga caggccaagg ggtacttcgg atgactctgt gcttgggcac agaaagaggt
     5881 cgtagtgtgc cccccgatag cggactcgac atgaatcaac taagaaaggc aatctgcctc
     5941 ccatgatgga cataagcaat agttcacaac catcttgcat ctcagtgaag tgtacataac
     6001 tattgagggc tgggtcatct aagcatttca gtcgagaaaa aaactgtaga ccaaaagaac
     6061 aactagcaac acttctcatc cagagaccca tatcaagatg ctagatccgg gagaggttta
     6121 tgatgaccct attgatccaa ttgagtcaga ggctgaaccc agaggaaccc ccactgtccc
     6181 caacatcttg aggaactccg actacaatct caattctcct ttgatagagg atcctgccaa
     6241 actaatgtta gaatggttga agacagggaa cagaccttat cggatgactt tgacagacaa
     6301 ttgctccagg tcttacaaag ttttgaaaga ttatttcaag aaagtagatt tgggttctct
     6361 caaagtgggc ggaactgctg cacagtcaat ggtttctctc tggttgtgtg gtgcccactc
     6421 tgaatcaaac aggagccgga gatgtataac cgacttggcc catttctatt ccaagtcatc
     6481 ccccatagag aagctattga attgtacgct aggaaacaga ggcctgagaa tcccaccaga
     6541 gggggtgtta aattgcctcg agagggtcaa ttatgacaag gcatttggga ggtatctggc
     6601 caacacgtat tcctcttact tgtttttcca tgtaatcacc ttatacatga atgccttaga
     6661 ctgggaagag gaaaagacca tcctggcatt atggaaagat ataacctcag tggataccga
     6721 gaaggacttg gtcaaattca aagatcaaat atggggactg ttgattgtga caaaggactt
     6781 tgtttactct cagagttcaa actgtctttt tgacagaaac tacacactga tgctaaagga
     6841 tcttttcttg tctcgattca actccttaat gattctgctt tctccccctg agccccgata
     6901 ctcagatgac ttaatatctc agctgtgcca gctatacatc gctggggatc aagtcttgtc
     6961 catgtgtggg aactccggct atgaagtcat caaaatattg gagccatatg tcgtgaacag
     7021 tttggtccag agggcagaga agtttaggcc tctcatccac cccttgggag actttcctat
     7081 gttcataaaa gacaaggtga atcaacttga agggactttt ggtcccagtg caaagaggtt
     7141 ttttagggtt ttagatcaat tcgacaacat acatgactta gtatttgtgt atggctgtta
     7201 cagacattgg gggcacccct atatagatta tcggaagggt ctgtcgaaac tatatgatca
     7261 agttcacatt aagaaagtaa tagataagtc ctaccaggag tgtttagcaa gtgacttggc
     7321 cagaaggatc ctcagatggg gatttgacaa gtactccaaa tggtatctag attcgagatt
     7381 ccttgcccga gaccacccct tgactcctta tgtcaagacc caaacatggc cacccaaaca
     7441 tatagtagac ttggtggggg acacatggca taagctcccg atcacgcaga tctttgaaat
     7501 tcctgaatca atggacccgt cagagatact ggatgacaaa tcacattctt tcaccagaac
     7561 aaggttagct tcttggctgt cagagaaccg aggggggcct gttcctagcg agaaggtcat
     7621 tatcacggcc ctgtctaagc cacctgtcaa tccccgagag tttttgaaat ctatagacct
     7681 cggaggattg ccagatgagg atttgataat tggcctcaaa ccaaaggaac gggagttgaa
     7741 gatcgagggc cgattctttg ctctaatgtc atggaatcta agattatatt ttgtcatcac
     7801 cgaaaagctc ttggccaact acattttgcc actttttgac gcactgacta tgacagacaa
     7861 cctgaacaag gtgttcaaaa agttgatcga cagggtcacc gggcaagggc ttttggacta
     7921 ttctagggtc acatacgcat ttcacctgga ctatgagaaa tggaacaatc atcaaagatt
     7981 ggagtcaaca gaggatgtat tctctgtcct agatcaggtg tttggattga agagggtgtt
     8041 ttctagaaca cacgagtttt ttcagaagtc ctggatctat tattcagaca gatcagacct
     8101 cattgggtta tgggaggacc aaatatattg cttggatatg tctaacggcc caacctgctg
     8161 gaatggccaa gatggcgggc tagagggctt gcggcagaag ggctggagtc tagtcagttt
     8221 attaatgata gatagagaat ctcaaaccag gaacacaaga accaagatac tagctcaagg
     8281 agacaaccag gttctgtgtc ctacatacat gttgtcaccg ggattgtctc aagaggggct
     8341 tctctatgag ttagagagca tatcaaggaa tgcactctca atataccgag ctatcgagga
     8401 aggagcatct aagctggggc tgatcatcaa gaaggaagag accatgtgta gttatgactt
     8461 tctcatatat gggaagaccc ccttatttcg aggcaacata ttggtgcctg aatccaaaag
     8521 atgggcccga gtctcttgca tctctaacga ccaaatagtc aacctcgcca atataatgtc
     8581 gacagtatcc accaatgcgc tgacagtggc acaacactct caatctctga tcaaacctat
     8641 gagggatttt ctgctcatgt cagtacaggc agttttccac tacctgttgt ttagcccaat
     8701 cctaaaaggc agagtttata agattctgag tgctgaaggg gagagctttc tcctagccat
     8761 gtcgcggata atctacctag atccttcttt gggaggggtg tctggaatgt ctctcgggag
     8821 gttccatata cgtcagttct cagaccctgt ctctgaaggg ttgtcattct ggagagagat
     8881 ctggttaggc tctcatgagt cctggattca cgcgttgtgt caggaggccg ggaaccccga
     8941 tcttggagag agaacactcg agagcttcac tcgcctttta gaagatccta ctaccttaaa
     9001 tatcaaagga ggggccagcc ctaccattct actcaaggat gctatcagaa aggctctgta
     9061 cgacgaggtg gacaaggtgg aaaattcaga gtttcgagag gcaatcctgc tgtccaagac
     9121 ccatagagat aactttatac tctttttaaa atctgttgag cctctgttcc ctcgatttct
     9181 cagtgagctc ttcagttcgt ctttcttggg aataccggag tcaatcattg gactgataca
     9241 aaactcccgg acaataagaa ggcagtttag aaagagtctc tcaagaactt tagaagagtc
     9301 cttctacaac tcagagatcc acgggattaa tcggataacc cagacacctc aaagggtcgg
     9361 aagggtgtgg ccttgctctt cagagagggc ggatctactt agggagatct cttgggggag
     9421 gaaagtggta ggcacgacag ttcctcaccc ttccgagatg ttggagttgt ttcccaaatc
     9481 ctccatttcc tgcacttgtg gagcaacagg gggaggcaat cctagagtct ctgtatcagt
     9541 actcccgtcc ttcgatcagt catttttctc acggggcccc ctaaagggat acttgggctc
     9601 gtccacctct atgtcaaccc agctattcca tgcatgggaa aaagtcacta atgttcatgt
     9661 ggtgaaaagg gctctatcgt taaaagaatc tataaactgg ttcatcaata ggaattccaa
     9721 tttggctcaa actctaattg gaaacatcat gtctctgaca ggccctgact tccctctaga
     9781 agaggcccct gttttcaaac ggacagggtc agccttgcat aggttcaagt ctgccagata
     9841 cagcgaagga gggtattctt ctgtttgccc taaccttctc tcccatatct ctgttagtac
     9901 agacactatg tctgatttga cccaaaacgg gaagaactat gatttcatgt ttcagccatt
     9961 gatgctttat gcgcaaacat ggacatcgga actggtacag agggatacaa gacttagaga
    10021 ctccacgttt cactggcacc ttcggtgcaa cagatgtgtg aggcccattg atgatataac
    10081 actggaaact tctcagatct tcgagttccc ggatgtgtca aaaaggatat ccaggatggt
    10141 ttctggagct gtccctcaat ttcagaagct tcctgatatc cgtctaagac caggtgattt
    10201 tgaatcccta agtggtagag aaaagtctcg ccatataggg tcagctcagg ggctcttata
    10261 ctcaatctta gtagcaattc acgactcagg atacaatgat gggaccatct tccctgtcaa
    10321 catatacggc aaagtttccc ccagagacta tttgagaggg cttgcaagag ggatcttgat
    10381 agggtcctcg atttgcttct tgacacgaat gacaaatatt aacattaaca gacctcttga
    10441 attgatctca ggggtaattt cctatattct cctgaggctg gataatcatc cctctctgta
    10501 tataatgctc agagaaccgt ctcttagagg agaaatattc tctatccctc agaaaatccc
    10561 cgccgcttac ccaaccacta tgagagaagg caacagatcg attttgtgtt acctccaaca
    10621 cgtgctacgc tatgagcgag aggcaatcac ggcgtccccg gagaatgact ggctgtggat
    10681 cttctcagac ttcagaagtg tgaaaatgac gtacttgacc ctcattactt accagtctca
    10741 cctcctactc cagagggttg agagaaactt gtctaagagt atgagagcta ctctgcgaca
    10801 aatgggttcc ttaatgaggc aagtgctggg tgggcacgga gaagatacct tggagtcaga
    10861 cgatgacatt caacgattac taaaagactc tttgcgaaag acaaggtggg tggatcaaga
    10921 ggtgcgccat gcagctagaa ccatgaatgg agattacagc cccgacaaga aagtatcccg
    10981 taaggtagga tgttcagaat gggtctgctc tgctcaacag attgccgtct ccacctcagc
    11041 caacccggcc cctgtctcag agcttgacat tagggccctc tctaagcggt ttcaaaaccc
    11101 cttgatctcg ggcctgagag tggttcagtg ggcaaccggt gcccattata agcttaagcc
    11161 tattctagat gatctaaatg ttttcccatc tctctgtctt gtaattggag acgggtcagg
    11221 gggaatatca agggcagttc tcaacatgtt tccagattct aagcttgtgt tcaacagcct
    11281 attggaggtg aatgatctga tggcttccgg aacacatcca ctgcctcctt cagcaatcat
    11341 gagtggagga gatgacatca tctccagagt gatagacttt gactcaatat gggagaaacc
    11401 gtccgacctg aggaactcgg ccacctggag atacttccag tcggttcaaa aacaggtcaa
    11461 catgtcgtat gacctcatta tttgtgatgc agaagttact gatattgcat ctatcaaccg
    11521 gataactctg ttgatgtctg atttcgcatt gtctatagat ggaccacttt atctggtctt
    11581 caaaacttac gggactatgc tagtaaaccc ggactataaa gctattcaac atctgtcaag
    11641 agcgttccct tcggtcacag ggtttgtaac ccaagtaact tcatcctttt cttctgagct
    11701 atacctccgg ttctctaaac gaggaaagtt tttcagggac gccgagtact tgacctcttc
    11761 cacccttcga gagatgagcc ttgtgttgtt caattgcagc agccccaaaa gtgagatgca
    11821 gagagctcgt tccttaaact atcaagacct ggtaagggga tttcctgaag agatcatatc
    11881 aaatccttac aacgaaatga tcataactct gattgacaat gatgtagagt ccttcctagt
    11941 ccacaagatg gtggatgatc ttgagctaca gaggggaact ctgtctaaag tggctatcat
    12001 tatatccatc atgatcgttt tttccaatag agtcttcaac atttccaaac ctttgactga
    12061 ccccttgttc tatccaccgt ctgatcctaa aatcctgagg cacttcaaca tatgttgcag
    12121 tactatgatg tatctatcta ccgctttagg cgacgtccct aacttcgcaa gacttcatga
    12181 cctgtataac agacctataa cttgttactt cagaaaacaa gttattcgag ggaatattta
    12241 tctatcttgg agttggtccg atgatacccc agtgttcaag agagtagcct gtaattctag
    12301 cttgagtctg tcatctcact ggatcaggtt gatctacaag atagtgaaga ctaccagact
    12361 cattggcagc ataaaagacc tatcaggaga ggtagaacga catcttcatg ggtataacag
    12421 atggatcacc ctcgaggata tccgatctag atcatcccta ctagattaca gttgtttgta
    12481 agccggatat taccgaaagc ctgtgcatgc taaaattctt gtatgatgca tcttgaaaaa
    12541 aacaagatct tgaatccgga cctctggttg tttgattgtt ttttccatct ttattgtttt
    12601 tttgttaagc gtgggtcggc atggcatctc cacctcctcg cggtccgacc tgggcatccg
    12661 aaggaggacg cacgtccact cggatggcta agggaggggc ggggatccgg ctgctaacaa
    12721 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
    12781 tggggcctct aaacgggtct tgaggggttt tttgctgaaa gtcgcgcttg gcgtaatcat
    12841 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    12901 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    12961 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    13021 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    13081 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    13141 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    13201 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    13261 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    13321 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    13381 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    13441 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    13501 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    13561 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    13621 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    13681 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    13741 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    13801 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    13861 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    13921 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    13981 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    14041 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    14101 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    14161 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    14221 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    14281 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    14341 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    14401 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    14461 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    14521 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    14581 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    14641 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    14701 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    14761 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    14821 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    14881 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    14941 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc
    15001 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
    15061 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
    15121 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
    15181 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
    15241 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
    15301 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
    15361 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
    15421 attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg
    15481 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    15541 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    15601 ggccagtgag cgcgcctagt tattaatagt aatcaattac ggggtcatta gttcatagcc
    15661 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
    15721 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
    15781 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
    15841 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
    15901 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
    15961 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
    16021 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
    16081 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
    16141 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
    16201 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
    16261 gctagattaa gcgtctgatg agtccgtgag gacgaaaccc ggcgtaccgg gtc
//
