LOCUS       Exported               15339 bp DNA     circular SYN 07-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 7, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15339
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             3325..4593
                     /codon_start=1
                     /label=GCaMP8f
                     /translation="MHHHHHHTRRKKTFKEVATAVKIIARLMGLKINVYIKADKQKNGI
                     KANFHIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVESKLSKDPNEKRDHMVL
                     LEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGE
                     GDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYI
                     QERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNLPDQLTEE
                     QIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGLNPTEAELQDMINEVDADGDGTIDFP
                     EFLTMMARKMKYRDTEEEIREAFGVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMI
                     READIDGDGQVNYEEFVQMMTAK"
     CDS             6768..6779
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(8571..8582)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      11793..11840
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(11868..11884)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11892..11908
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11916..11946)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11961..11982
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(12270..12858)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(13029..13889)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13890..13994)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14021..14476
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     14617..14633
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        14703..15006
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15007..15210
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        15255..15273
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatgcat catcaccatc atcacacgcg tcgcaagaag
     3361 accttcaagg aggtggccac cgccgtgaag atcatcgccc gcctgatggg cctgaagatc
     3421 aacgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt ccacatccgc
     3481 cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa cacccccatc
     3541 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtggagtc caaactttcg
     3601 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     3661 atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat ggtgagcaag
     3721 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     3781 ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg caagctgacc
     3841 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     3901 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     3961 ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt caaggacgac
     4021 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     4081 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     4141 aacctgccgg accaactgac tgaagagcag atcgcagaat ttaaagaggc tttctcccta
     4201 tttgacaagg acggggatgg gacaataaca accaaggagc tggggacggt gatgcggtct
     4261 ctcggactga accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac
     4321 ggtgacggca caatcgactt ccctgagttc ctgacaatga tggcaagaaa aatgaaatac
     4381 agggacacgg aagaagaaat tagagaagcg ttcggtgtgt ttgataagga tggcaatggc
     4441 tacatcagtg cagcagagct tcgccacgtg atgacaaacc ttggagagaa gttaacagat
     4501 gaagaggttg atgaaatgat cagggaagca gacatcgatg gggatggtca ggtaaactac
     4561 gaagagtttg tacaaatgat gacagcgaag taagctagcg gcgcctagcc ggtcatcctt
     4621 ttgacgattc cagtcccgag gataacctcc tctcgggatt ggggggaatc tttggatcca
     4681 gtagtcctcc ttgaactccg tccaacaggg cagattcaag agtcataaga ctttcattaa
     4741 tcatttcagt tgatcagaca tggtcgtgta gattctctta atacgggaga tcttctagca
     4801 gtttcagtga ccaacggtgc tttcattctc caggaactga taccaaaggt tgtggacagg
     4861 ccaaggggta cttcggatga ctctgtgctt gggcacagaa agaggtcgta gtgtgccccc
     4921 cgatagcgga ctcgacatga atcaactaag aaaggcaatc tgcctcccat gatggacata
     4981 agcaatagtt cacaaccatc ttgcatctca gtgaagtgta cataactatt gagggctggg
     5041 tcatctaagc atttcagtcg agaaaaaaac tgtagaccaa aagaacaact agcaacactt
     5101 ctcatccaga gacccatatc aagatgctag atccgggaga ggtttatgat gaccctattg
     5161 atccaattga gtcagaggct gaacccagag gaacccccac tgtccccaac atcttgagga
     5221 actccgacta caatctcaat tctcctttga tagaggatcc tgccaaacta atgttagaat
     5281 ggttgaagac agggaacaga ccttatcgga tgactttgac agacaattgc tccaggtctt
     5341 acaaagtttt gaaagattat ttcaagaaag tagatttggg ttctctcaaa gtgggcggaa
     5401 ctgctgcaca gtcaatggtt tctctctggt tgtgtggtgc ccactctgaa tcaaacagga
     5461 gccggagatg tataaccgac ttggcccatt tctattccaa gtcatccccc atagagaagc
     5521 tattgaattg tacgctagga aacagaggcc tgagaatccc accagagggg gtgttaaatt
     5581 gcctcgagag ggtcaattat gacaaggcat ttgggaggta tctggccaac acgtattcct
     5641 cttacttgtt tttccatgta atcaccttat acatgaatgc cttagactgg gaagaggaaa
     5701 agaccatcct ggcattatgg aaagatataa cctcagtgga taccgagaag gacttggtca
     5761 aattcaaaga tcaaatatgg ggactgttga ttgtgacaaa ggactttgtt tactctcaga
     5821 gttcaaactg tctttttgac agaaactaca cactgatgct aaaggatctt ttcttgtctc
     5881 gattcaactc cttaatgatt ctgctttctc cccctgagcc ccgatactca gatgacttaa
     5941 tatctcagct gtgccagcta tacatcgctg gggatcaagt cttgtccatg tgtgggaact
     6001 ccggctatga agtcatcaaa atattggagc catatgtcgt gaacagtttg gtccagaggg
     6061 cagagaagtt taggcctctc atccacccct tgggagactt tcctatgttc ataaaagaca
     6121 aggtgaatca acttgaaggg acttttggtc ccagtgcaaa gaggtttttt agggttttag
     6181 atcaattcga caacatacat gacttagtat ttgtgtatgg ctgttacaga cattgggggc
     6241 acccctatat agattatcgg aagggtctgt cgaaactata tgatcaagtt cacattaaga
     6301 aagtaataga taagtcctac caggagtgtt tagcaagtga cttggccaga aggatcctca
     6361 gatggggatt tgacaagtac tccaaatggt atctagattc gagattcctt gcccgagacc
     6421 accccttgac tccttatgtc aagacccaaa catggccacc caaacatata gtagacttgg
     6481 tgggggacac atggcataag ctcccgatca cgcagatctt tgaaattcct gaatcaatgg
     6541 acccgtcaga gatactggat gacaaatcac attctttcac cagaacaagg ttagcttctt
     6601 ggctgtcaga gaaccgaggg gggcctgttc ctagcgagaa ggtcattatc acggccctgt
     6661 ctaagccacc tgtcaatccc cgagagtttt tgaaatctat agacctcgga ggattgccag
     6721 atgaggattt gataattggc ctcaaaccaa aggaacggga gttgaagatc gagggccgat
     6781 tctttgctct aatgtcatgg aatctaagat tatattttgt catcaccgaa aagctcttgg
     6841 ccaactacat tttgccactt tttgacgcac tgactatgac agacaacctg aacaaggtgt
     6901 tcaaaaagtt gatcgacagg gtcaccgggc aagggctttt ggactattct agggtcacat
     6961 acgcatttca cctggactat gagaaatgga acaatcatca aagattggag tcaacagagg
     7021 atgtattctc tgtcctagat caggtgtttg gattgaagag ggtgttttct agaacacacg
     7081 agttttttca gaagtcctgg atctattatt cagacagatc agacctcatt gggttatggg
     7141 aggaccaaat atattgcttg gatatgtcta acggcccaac ctgctggaat ggccaagatg
     7201 gcgggctaga gggcttgcgg cagaagggct ggagtctagt cagtttatta atgatagata
     7261 gagaatctca aaccaggaac acaagaacca agatactagc tcaaggagac aaccaggttc
     7321 tgtgtcctac atacatgttg tcaccgggat tgtctcaaga ggggcttctc tatgagttag
     7381 agagcatatc aaggaatgca ctctcaatat accgagctat cgaggaagga gcatctaagc
     7441 tggggctgat catcaagaag gaagagacca tgtgtagtta tgactttctc atatatggga
     7501 agaccccctt atttcgaggc aacatattgg tgcctgaatc caaaagatgg gcccgagtct
     7561 cttgcatctc taacgaccaa atagtcaacc tcgccaatat aatgtcgaca gtatccacca
     7621 atgcgctgac agtggcacaa cactctcaat ctctgatcaa acctatgagg gattttctgc
     7681 tcatgtcagt acaggcagtt ttccactacc tgttgtttag cccaatccta aaaggcagag
     7741 tttataagat tctgagtgct gaaggggaga gctttctcct agccatgtcg cggataatct
     7801 acctagatcc ttctttggga ggggtgtctg gaatgtctct cgggaggttc catatacgtc
     7861 agttctcaga ccctgtctct gaagggttgt cattctggag agagatctgg ttaggctctc
     7921 atgagtcctg gattcacgcg ttgtgtcagg aggccgggaa ccccgatctt ggagagagaa
     7981 cactcgagag cttcactcgc cttttagaag atcctactac cttaaatatc aaaggagggg
     8041 ccagccctac cattctactc aaggatgcta tcagaaaggc tctgtacgac gaggtggaca
     8101 aggtggaaaa ttcagagttt cgagaggcaa tcctgctgtc caagacccat agagataact
     8161 ttatactctt tttaaaatct gttgagcctc tgttccctcg atttctcagt gagctcttca
     8221 gttcgtcttt cttgggaata ccggagtcaa tcattggact gatacaaaac tcccggacaa
     8281 taagaaggca gtttagaaag agtctctcaa gaactttaga agagtccttc tacaactcag
     8341 agatccacgg gattaatcgg ataacccaga cacctcaaag ggtcggaagg gtgtggcctt
     8401 gctcttcaga gagggcggat ctacttaggg agatctcttg ggggaggaaa gtggtaggca
     8461 cgacagttcc tcacccttcc gagatgttgg agttgtttcc caaatcctcc atttcctgca
     8521 cttgtggagc aacaggggga ggcaatccta gagtctctgt atcagtactc ccgtccttcg
     8581 atcagtcatt tttctcacgg ggccccctaa agggatactt gggctcgtcc acctctatgt
     8641 caacccagct attccatgca tgggaaaaag tcactaatgt tcatgtggtg aaaagggctc
     8701 tatcgttaaa agaatctata aactggttca tcaataggaa ttccaatttg gctcaaactc
     8761 taattggaaa catcatgtct ctgacaggcc ctgacttccc tctagaagag gcccctgttt
     8821 tcaaacggac agggtcagcc ttgcataggt tcaagtctgc cagatacagc gaaggagggt
     8881 attcttctgt ttgccctaac cttctctccc atatctctgt tagtacagac actatgtctg
     8941 atttgaccca aaacgggaag aactatgatt tcatgtttca gccattgatg ctttatgcgc
     9001 aaacatggac atcggaactg gtacagaggg atacaagact tagagactcc acgtttcact
     9061 ggcaccttcg gtgcaacaga tgtgtgaggc ccattgatga tataacactg gaaacttctc
     9121 agatcttcga gttcccggat gtgtcaaaaa ggatatccag gatggtttct ggagctgtcc
     9181 ctcaatttca gaagcttcct gatatccgtc taagaccagg tgattttgaa tccctaagtg
     9241 gtagagaaaa gtctcgccat atagggtcag ctcaggggct cttatactca atcttagtag
     9301 caattcacga ctcaggatac aatgatggga ccatcttccc tgtcaacata tacggcaaag
     9361 tttcccccag agactatttg agagggcttg caagagggat cttgataggg tcctcgattt
     9421 gcttcttgac acgaatgaca aatattaaca ttaacagacc tcttgaattg atctcagggg
     9481 taatttccta tattctcctg aggctggata atcatccctc tctgtatata atgctcagag
     9541 aaccgtctct tagaggagaa atattctcta tccctcagaa aatccccgcc gcttacccaa
     9601 ccactatgag agaaggcaac agatcgattt tgtgttacct ccaacacgtg ctacgctatg
     9661 agcgagaggc aatcacggcg tccccggaga atgactggct gtggatcttc tcagacttca
     9721 gaagtgtgaa aatgacgtac ttgaccctca ttacttacca gtctcacctc ctactccaga
     9781 gggttgagag aaacttgtct aagagtatga gagctactct gcgacaaatg ggttccttaa
     9841 tgaggcaagt gctgggtggg cacggagaag ataccttgga gtcagacgat gacattcaac
     9901 gattactaaa agactctttg cgaaagacaa ggtgggtgga tcaagaggtg cgccatgcag
     9961 ctagaaccat gaatggagat tacagccccg acaagaaagt atcccgtaag gtaggatgtt
    10021 cagaatgggt ctgctctgct caacagattg ccgtctccac ctcagccaac ccggcccctg
    10081 tctcagagct tgacattagg gccctctcta agcggtttca aaaccccttg atctcgggcc
    10141 tgagagtggt tcagtgggca accggtgccc attataagct taagcctatt ctagatgatc
    10201 taaatgtttt cccatctctc tgtcttgtaa ttggagacgg gtcaggggga atatcaaggg
    10261 cagttctcaa catgtttcca gattctaagc ttgtgttcaa cagcctattg gaggtgaatg
    10321 atctgatggc ttccggaaca catccactgc ctccttcagc aatcatgagt ggaggagatg
    10381 acatcatctc cagagtgata gactttgact caatatggga gaaaccgtcc gacctgagga
    10441 actcggccac ctggagatac ttccagtcgg ttcaaaaaca ggtcaacatg tcgtatgacc
    10501 tcattatttg tgatgcagaa gttactgata ttgcatctat caaccggata actctgttga
    10561 tgtctgattt cgcattgtct atagatggac cactttatct ggtcttcaaa acttacggga
    10621 ctatgctagt aaacccggac tataaagcta ttcaacatct gtcaagagcg ttcccttcgg
    10681 tcacagggtt tgtaacccaa gtaacttcat ccttttcttc tgagctatac ctccggttct
    10741 ctaaacgagg aaagtttttc agggacgccg agtacttgac ctcttccacc cttcgagaga
    10801 tgagccttgt gttgttcaat tgcagcagcc ccaaaagtga gatgcagaga gctcgttcct
    10861 taaactatca agacctggta aggggatttc ctgaagagat catatcaaat ccttacaacg
    10921 aaatgatcat aactctgatt gacaatgatg tagagtcctt cctagtccac aagatggtgg
    10981 atgatcttga gctacagagg ggaactctgt ctaaagtggc tatcattata tccatcatga
    11041 tcgttttttc caatagagtc ttcaacattt ccaaaccttt gactgacccc ttgttctatc
    11101 caccgtctga tcctaaaatc ctgaggcact tcaacatatg ttgcagtact atgatgtatc
    11161 tatctaccgc tttaggcgac gtccctaact tcgcaagact tcatgacctg tataacagac
    11221 ctataacttg ttacttcaga aaacaagtta ttcgagggaa tatttatcta tcttggagtt
    11281 ggtccgatga taccccagtg ttcaagagag tagcctgtaa ttctagcttg agtctgtcat
    11341 ctcactggat caggttgatc tacaagatag tgaagactac cagactcatt ggcagcataa
    11401 aagacctatc aggagaggta gaacgacatc ttcatgggta taacagatgg atcaccctcg
    11461 aggatatccg atctagatca tccctactag attacagttg tttgtaagcc ggatattacc
    11521 gaaagcctgt gcatgctaaa attcttgtat gatgcatctt gaaaaaaaca agatcttgaa
    11581 tccggacctc tggttgtttg attgtttttt ccatctttat tgtttttttg ttaagcgtgg
    11641 gtcggcatgg catctccacc tcctcgcggt ccgacctggg catccgaagg aggacgcacg
    11701 tccactcgga tggctaaggg aggggcgggg atccggctgc taacaaagcc cgaaaggaag
    11761 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
    11821 gggtcttgag gggttttttg ctgaaagtcg cgcttggcgt aatcatggtc atagctgttt
    11881 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
    11941 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
    12001 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
    12061 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
    12121 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    12181 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    12241 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    12301 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    12361 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    12421 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    12481 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    12541 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    12601 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    12661 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
    12721 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    12781 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    12841 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    12901 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    12961 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    13021 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    13081 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    13141 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    13201 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    13261 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    13321 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    13381 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    13441 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    13501 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    13561 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    13621 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    13681 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    13741 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    13801 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    13861 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    13921 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    13981 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca
    14041 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
    14101 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
    14161 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
    14221 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
    14281 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    14341 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    14401 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    14461 ttaacgctta caatttccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    14521 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    14581 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg
    14641 cctagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
    14701 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
    14761 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
    14821 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
    14881 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
    14941 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
    15001 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
    15061 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
    15121 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
    15181 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
    15241 tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta gattaagcgt
    15301 ctgatgagtc cgtgaggacg aaacccggcg taccgggtc
//
