LOCUS       Exported                4719 bp ds-DNA     circular SYN 08-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 8, 2021 from SnapGene 5.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    1..34
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     regulatory      207..216
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             213..1469
                     /codon_start=1
                     /product="BC069482
                     "
                     /label=Human Kir3.4 (KCNJ5)
                     /translation="MAGDSRNAMNQDMEIGVTPWDPKKIPKQARDYVPIATDRTRLLAE
                     GKKPRQRYMEKSGKCNVHHGNVQETYRYLSDLFTTLVDLKWRFNLLVFTMVYTVTWLFF
                     GFIWWLIAYIRGDLDHVGDQEWIPCVENLSGFVSAFLFSIETETTIGYGFRVITEKCPE
                     GIILLLVQAILGSIVNAFMVGCMFVKISQPKKRAETLMFSNNAVISMRDEKLCLMFRVG
                     DLRNSHIVEASIRAKLIKSRQTKEGEFIPLNQTDINVGFDTGDDRLFLVSPLIISHEIN
                     EKSPFWEMSQAQLHQEEFEVVVILEGMVEATGMTCQARSSYMDTEVLWGHRFTPVLTLE
                     KGFYEVDYNTFHDTYETNTPSCCAKELAEMKREGRLLQYLPSPPLLGGCAEAGLDAEAE
                     QNEEDEPKGLGGSREARGSV"
     CDS             1479..1499
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1506..2210
                     /codon_start=1
                     /label=mCherry_pichia_codon_optimized
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     CDS             2226..2249
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    2276..2410
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  2531..2696
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2886..3474
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  3609..3832
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3899..4432)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(4621..4649)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa ataaccgcca ccatggccgg cgattctagg aatgccatga
      241 accaggacat ggagattgga gtcactccct gggaccccaa gaagattcca aaacaggccc
      301 gcgattatgt ccccattgcc acagaccgta cgcgcctgct ggccgagggc aagaagccac
      361 gccagcgcta catggagaag agcggcaagt gcaacgtgca ccacggcaac gtccaggaga
      421 cctaccggta cctgagtgac ctcttcacca ccctggtgga cctcaagtgg cgcttcaact
      481 tgctcgtctt caccatggtt tacactgtca cctggctgtt cttcggcttc atttggtggc
      541 tcattgctta tatccggggt gacctggacc atgttggcga ccaagagtgg attccttgtg
      601 ttgaaaacct cagtggcttc gtgtccgctt tcctgttctc cattgagacc gaaacaacca
      661 ttgggtatgg cttccgagtc atcacagaga agtgtccaga ggggattata ctcctcttgg
      721 tccaggccat cctgggctcc atcgtcaatg ccttcatggt ggggtgcatg tttgtcaaga
      781 tcagccagcc caagaagaga gcggagaccc tcatgttttc caacaacgca gtcatctcca
      841 tgcgggacga gaagctgtgc ctcatgttcc gggtgggcga cctccgcaac tcccacatcg
      901 tggaggcctc catccgggcc aagctcatca agtcccggca gaccaaagag ggggagttca
      961 tccccctgaa ccagacagac atcaacgtgg gctttgacac gggcgacgac cgcctcttcc
     1021 tggtgtctcc tctgatcatc tcccacgaga tcaacgagaa gagccctttc tgggagatgt
     1081 ctcaggctca gctgcatcag gaagagtttg aagttgtggt cattctagaa gggatggtgg
     1141 aagccacagg catgacctgc caagcccgga gctcctacat ggatacagag gtgctctggg
     1201 gccaccgatt cacaccagtc ctcaccttgg aaaagggctt ctatgaggtg gactacaaca
     1261 ccttccatga tacctatgag accaacacac ccagctgctg tgccaaggag ctggcagaaa
     1321 tgaagaggga aggccggctc ctccagtacc tccccagccc cccactgctg gggggctgtg
     1381 ctgaggcagg gctggatgca gaggctgagc agaatgaaga agatgagccc aaggggctgg
     1441 gtgggtccag ggaggccagg ggctcggtgg gtaccagcga aaacctgtat tttcagggta
     1501 ctagtgtctc taagggagaa gaggacaata tggctattat caaggagttc atgagattta
     1561 aagtccatat ggagggctct gttaatggtc atgaatttga aattgaaggt gaaggagaag
     1621 gaagacctta tgagggaacc caaacggcta agctgaaagt aactaaaggc ggcccacttc
     1681 ctttcgcatg ggatatactg tctccacaat tcatgtatgg ctctaaagct tatgtaaagc
     1741 accccgccga cattcccgat tatctaaagt tatcctttcc cgagggattt aaatgggaaa
     1801 gggttatgaa cttcgaggat ggcggcgtgg taactgttac gcaggactcc tcccttcaag
     1861 atggtgaatt tatttacaaa gtgaagcttc gtggtactaa tttcccaagt gacggtcctg
     1921 tgatgcagaa gaagactatg ggttgggaag ctagttccga gaggatgtac cccgaagatg
     1981 gtgccctgaa gggtgagatt aagcaacgtc taaaattaaa agacggcggt cactatgacg
     2041 ctgaagtaaa gaccacatat aaggcaaaaa agcccgtcca gcttcctggc gcctacaatg
     2101 ttaatatcaa gttggacatc acctcccata acgaagatta cactatcgtc gagcaatacg
     2161 agagggcaga gggaagacat tccacaggtg gtatggacga gctatataaa ctcgagggcg
     2221 cttcctggtc acatccacag tttgagaaat aataatgaat gcaattgttg ttgttaactt
     2281 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     2341 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     2401 tgtctggatc tgatcactgc ttgagcctag aagatccggc tgctaacaaa gcccgaaagg
     2461 aagctgagtt ggctgctgcc accgctgagc aataactatc ataaccccta gggtataccc
     2521 atctaattgg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt
     2581 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa
     2641 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacaaccg
     2701 gttgacttgg gtcaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2761 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2821 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2881 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2941 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3001 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3061 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3121 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3181 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3241 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3301 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3361 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3421 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3481 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3541 cctgcgttat cccctgattg acttgggtcg ctcttcctgt ggatgcgcag atgccctgcg
     3601 taagcgggtg tgggcggaca ataaagtctt aaactgaaca aaatagatct aaactatgac
     3661 aataaagtct taaactagac agaatagttg taaactgaaa tcagtccagt tatgctgtga
     3721 aaaagcatac tggacttttg ttatggctaa agcaaactct tcattttctg aagtgcaaat
     3781 tgcccgtcgt attaaagagg ggcgtggcca agggcatgta aagactatat tcgcggcgtt
     3841 gtgacaattt accgaacaac tccgcggccg ggaagccgat ctcggcttga acgaattgtt
     3901 aggtggcggt acttgggtcg atatcaaagt gcatcacttc ttcccgtatg cccaactttg
     3961 tatagagagc cactgcggga tcgtcaccgt aatctgcttg cacgtagatc acataagcac
     4021 caagcgcgtt ggcctcatgc ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc
     4081 ggtgctcgcc ggagactgcg agatcataga tatagatctc actacgcggc tgctcaaact
     4141 tgggcagaac gtaagccgcg agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg
     4201 cgatgaatgt cttactacgg agcaagttcc cgaggtaatc ggagtccggc tgatgttggg
     4261 agtaggtggc tacgtctccg aactcacgac cgaaaagatc aagagcagcc cgcatggatt
     4321 tgacttggtc agggccgagc ctacatgtgc gaatgatgcc catacttgag ccacctaact
     4381 ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctgcgtaac atcgttgctg
     4441 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     4501 atagactgta caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
     4561 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac
     4621 agtttacgaa ccgaacaggc ttatgtcaac tgggttcgtg ccttcatccg tttccacggt
     4681 gtgcgtcacc cggcaacctt gggcagcagc gaagtcgcc
//
