LOCUS       Exported               10609 bp DNA     circular SYN 12-AUG-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10609)
  AUTHORS   Collins Lab
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10609
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_structure  2028..2151
                     /label=cPPT
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    2028..2144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
                     /note="color: #ffe4c4"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
                     /note="color: #ffe4c4"
     promoter        2209..3380
                     /label=EF-1a
                     /note="color: #66cc99; direction: RIGHT"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     intron          3168..3371
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
                     /note="color: #------"
     primer_bind     3362..3416
                     /label=ProlacPP1_4SC160_F
                     /note="color: black; sequence: 
                     tccatttcaggtgtcgtgaggatccACGAGAATGGACTCTAAGGGCAGCAGCCAG"
     primer_bind     complement(3364..3392)
                     /label=SC160_vectV2_R
                     /note="color: black; sequence: 
                     TCTCGTggatcctcacgacacctgaaatg"
     misc_feature    3381
                     /label=MCS
                     /note="color: #00ccff"
     misc_feature    3393..3482
                     /label=ProlactinSS-CodonOpt
                     /note="color: #33cccc; direction: RIGHT"
     misc_feature    3483..4505
                     /label=CodonOpt-PP1
                     /note="color: #800080; direction: RIGHT"
     primer_bind     complement(3753..3766)
                     /label=PP1_qPCR_R
                     /note="color: black; sequence: actctgcccaggctgaagta"
     CDS             4506..4532
                     /codon_start=1
                     /label=Linker
                     /note="color: #00ff00"
                     /translation="ETSQVAPAE"
     primer_bind     complement(4514..4554)
                     /label=ProlactPP1_4Sc160_R
                     /note="color: black; sequence: 
                     cctcgcccttgctcacTTCAGCAGGGGCCACCTGG"
     primer_bind     4522..4559
                     /label=mCit_4PP1-Sc160V2_F
                     /note="color: black; sequence: 
                     CCCCTGCTGAAgtgagcaagggcgaggagctg"
     CDS             4539..5252
                     /label=mCitrine
                     /note="color: #ffff00; direction: RIGHT"
     primer_bind     complement(4825..4844)
                     /label=mCit_Seq_R
                     /note="color: black; sequence: gtccttgaagaagatggtgc"
     primer_bind     4825..4844
                     /label=mCit_seq_F
                     /note="color: black; sequence: gcaccatcttcttcaaggac"
     primer_bind     complement(5078..5095)
                     /label=mCit_Col_R
                     /note="color: black; sequence: gtgttctgctggtagtgg"
     primer_bind     complement(5229..5270)
                     /label=mCit_4PP1-Sc160_R
                     /note="color: black; sequence: 
                     ctctagagcggccgcTCActtgtacagctcgtccatgccgag"
     protein_bind    5252
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     5256..5275
                     /label=SC160_vect_F
                     /note="color: black; sequence: gcggccgctctagagtcgac"
     misc_feature    5262
                     /label=IRES
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    5293..5904
                     /label=IRES
                     /note="color: #a6acb3"
     CDS             5908..6507
                     /codon_start=1
                     /label=Puro
                     /note="color: #ff9966"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    6531..7122
                     /label=WPRE
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    6531..7119
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(7002..7013)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 7001"
                     /translation="IEGR"
     LTR             7327..7960
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     complement(8089..8105)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    8113..8129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(8137..8167)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 8137 .. 8143 / #ffffff / -10
                        2: 8144 .. 8161 / #ffffff
                        3: 8162 .. 8167 / #ffffff / -35"
     protein_bind    8182..8203
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(8491..9076)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(9247..10107)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  9247 .. 10038 / #ccffcc
                        2: 10039 .. 10107 / #ccffcc / signal sequence
                     Cleavage site after base 10038"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10108..10212)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    10260..10394
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccacga gaatggactc taagggcagc agccagaagg
     3421 gctcccggct gctgctcctc ctcgtggtga gcaacctgct gctctgccag ggggtggtgt
     3481 ctatgcacga ggagatggag tccgagacct ctacagctgc ctctgggtcc attgctgagg
     3541 tgatgccaag aatggggtac acaatcctcg ccgtgattat cggggtgttc tccgtgtgcg
     3601 gagtgatcct caacgtgaca gtgatcacag tgaccctcaa gtacaagcag ctgaggcagc
     3661 ccctcaactt cgccctggtg aacctcgctg tggctgacct cggctgcgct gtgttcggag
     3721 gcctgcccac cgtggtgacc aacgctatgg gctacttcag cctgggacgg gtggggtgcg
     3781 tgctggaggg cttcgctgtg gccttcttcg gcattgctgc cctctgctct gtggccgtga
     3841 ttgccctcga gcgctgcatg gtggtgtgcc gcccagtggg ctccatttct ttccagacaa
     3901 ggcacgctgt gttcggagtg gccgtgtcct ggctctggtc cttcatctgg aacacccctc
     3961 ccctcttcgg atgggggagg ttcgagctcg agggagtgag aacatcttgc gctcctgact
     4021 ggtactctag ggacctggcc aacgtgtctt tcatcgtgtg ctacttcctg ctgtgcttcg
     4081 ccctcccatt cagcgtgatt gtgtactctt acaccagact cctgtggaca ctgcggcagg
     4141 tgtctagact gcaggtgtgc gagggggggt ccgctgctag ggctgaggct caggtgtcct
     4201 gcatggtggt ggtgatgatt ctggccttcc tcctgacatg gctgccatac gctagcttcg
     4261 ctctctgcgt gatcctcatt cccgagctgt acattgaccc tgtgattgct accgtgccca
     4321 tgtacctgac caagtcttcc acagtgttca accctatcat ctacatcttc atgaacaggc
     4381 agttcagaga cagagctctc cccttcctcc tgtgcggacg caacccctgg gccgctgagg
     4441 ccgaggagga ggaggaggag acaaccgtgt ctagcgtgag ccggtccacc tccgtgtctc
     4501 ctgctgagac atcccaggtg gcccctgctg aaatgcatgt gagcaagggc gaggagctgt
     4561 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     4621 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     4681 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc
     4741 tgatgtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     4801 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     4861 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     4921 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     4981 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     5041 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     5101 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga
     5161 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     5221 ggatcactct cggcatggac gagctgtaca agtgagcggc cgctctagag tcgacgggcc
     5281 gcggtaacaa ttgttaacta acttaagcta gcaacggttt ccctctagcg ggatcaattc
     5341 cgcccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     5401 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     5461 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     5521 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     5581 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     5641 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     5701 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     5761 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     5821 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     5881 ttttcctttg aaaaacacga taataccatg accgagtaca agcccacggt gcgcctcgcc
     5941 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     6001 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     6061 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6121 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     6181 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     6241 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     6301 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     6361 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     6421 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     6481 tgcatgaccc gcaagcccgg tgcctgaccc gggcggggcg cgtctggaac aatcaacctc
     6541 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6601 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6661 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6721 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6781 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6841 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6901 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     6961 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7021 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7081 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg
     7141 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     7201 tgcctggcta gaagcacaag aggaggagga ggtgggtttt tccagtcaca cctcaggtac
     7261 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga
     7321 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     7381 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
     7441 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
     7501 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
     7561 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
     7621 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
     7681 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
     7741 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
     7801 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7861 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7921 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     7981 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat
     8041 tgctagcgtt ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     8101 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8161 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8221 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8281 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8341 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8401 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8461 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8521 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8581 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8641 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8701 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8761 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8821 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8881 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8941 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9001 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag
     9061 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9121 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9181 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9241 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9301 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9361 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9421 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9481 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9541 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9601 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9661 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9721 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9781 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9841 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9901 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9961 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10021 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10081 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10141 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10201 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca
    10261 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    10321 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10381 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    10441 cataccctat taccactgcc aattacctag tggtttcatt tactctaaac ctgtgattcc
    10501 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    10561 gtttttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
