LOCUS       Exported               11550 bp DNA     circular SYN 28-JAN-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11550)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11550
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_structure  2028..2151
                     /label=cPPT
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    2028..2144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
                     /note="color: #ffe4c4"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
                     /note="color: #ffe4c4"
     primer_bind     2054..2071
                     /label=cPPT_Seq_F
                     /note="color: black; sequence: GTACAGTGCAGGGGAAAG"
     primer_bind     2184..2234
                     /label=UCOE_F_V2
                     /note="color: black; sequence: 
                     tgaggccctttcgtcttcacatatgcgggaggtggtccctgcagttacgcc"
     misc_feature    2209..2888
                     /label=UCOE
                     /note="This feature has 3 segments:
                        1: 2209 .. 2820 / #ff0000
                        2: 2821 .. 2829 / #0000ff / Not present in the plasmid
                        3: 2830 .. 2888 / #ff0000"
     misc_feature    2209..2449
                     /label=minUCOE
                     /label=nonstandard type:  chromatin
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
                     /note="color: #c6c9d1"
     misc_feature    complement(2223..2231)
                     /label=CTCF binding site
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
                     /note="color: #85dae9; direction: LEFT"
     primer_bind     2320..2338
                     /label=UCOE_Colony.FOR
                     /note="color: black; sequence: cggaaacacccgaatcaac"
     misc_feature    2398..2411
                     /label=CTCF binding site
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
                     /note="color: #85dae9"
     misc_feature    2450..2888
                     /label=UCOE
                     /note="/ApEinfo_fwdcolor=#b4abac
                     /ApEinfo_revcolor=#b4abac"
                     /note="color: #b4abac"
     primer_bind     complement(2660..2676)
                     /label=UCOE_Colony.REV
                     /note="color: black; sequence: cgcggcacttaccgttc"
     mRNA            2807..2888
                     /label=CBX3 mRNA
                     /note="/ApEinfo_fwdcolor=#b4abac
                     /ApEinfo_revcolor=#b4abac"
                     /note="color: #b4abac; direction: RIGHT"
     misc_feature    2821..2829
                     /label=missing in actual plasmid
                     /note="/ApEinfo_fwdcolor=#ffef86
                     /ApEinfo_revcolor=#ffef86"
                     /note="color: #ffef86"
     primer_bind     complement(2865..2914)
                     /label=UCOE_R_V3
                     /note="color: black; sequence: 
                     tctgcccactgacgggcacctcgagtctccgcgcctacagctcaagccac"
     misc_feature    2888
                     /label=origin
                     /note="/ApEinfo_fwdcolor=#b4abac
                     /ApEinfo_revcolor=#b4abac"
                     /note="color: #b4abac"
     misc_feature    2895..2914
                     /label=origin
                     /note="/ApEinfo_fwdcolor=#b4abac
                     /ApEinfo_revcolor=#b4abac"
                     /note="color: #b4abac"
     misc_feature    2895..2914
                     /label=EFS
                     /note="/ApEinfo_fwdcolor=#b4abac
                     /ApEinfo_revcolor=#b4abac"
                     /note="color: #b4abac"
     promoter        2896..4067
                     /label=EF-1a
                     /note="color: #66cc99; direction: RIGHT"
     promoter        2908..3119
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(2951..2968)
                     /label=EF1a_begin_seq.REV
                     /note="color: black; sequence: gttcaattgccgacccct"
     intron          3120..4058
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     primer_bind     complement(3829..3838)
                     /label=mTurq2_Col_F
                     /note="color: black; sequence: ctggtcgagctggacggcg"
     intron          3855..4058
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
                     /note="color: #------"
     primer_bind     3865..3884
                     /label=EF-1a_seq.F
                     /note="color: black; sequence: ggagtacgtcgtctttaggt"
     primer_bind     4051..4104
                     /label=iRFP_4SC627_F
                     /note="color: black; sequence: 
                     catttcaggtgtcgtgagGagcgctatggcgcgtaaggtcgatctcacctcctg; 
                     added: 2019-10-25"
     CDS             4076..5008
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /note="color: #a0003c"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     primer_bind     4124..4143
                     /label=iRFP_col_F
                     /note="color: black; sequence: atccccggcagcattcagcc; added:
                     2020-01-28"
     primer_bind     complement(4430..4450)
                     /label=iRFP_col_R
                     /note="color: black; sequence: cggattgtcggcctgttcggc; 
                     added: 2020-01-29"
     primer_bind     4616..4636
                     /label=iRFP-CAAX seq.FOR
                     /note="color: black; sequence: cgcagcgacctcgagagcttt; 
                     added: 2019-11-08"
     primer_bind     4677..4695
                     /label=iRFP-CAAX seq2.FOR
                     /note="color: black; sequence: cgcggctactgtacttgaa"
     primer_bind     4855..4873
                     /label=iRFP-CAAX seq3.FOR
                     /note="color: black; sequence: gatgtcgctgtcgatcatc"
     primer_bind     complement(4990..5035)
                     /label=iRFP_4SC627_R
                     /note="color: black; sequence: 
                     gttcgtggcgccgctgcctGCacttccgcgttggtggtgggcggcg; added: 
                     2019-10-25"
     primer_bind     4998..5040
                     /label=P2AT2A_4SC627_F
                     /note="color: black; sequence: 
                     accaccaacgcGGAAGTGCAggcagcggcgccacgaacttctc; added: 
                     2020-01-08"
     CDS             5018..5083
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="This feature has 2 segments:
                        1: 5018 .. 5026 / #cc99b2 / P2A_improves cleavage
                        2: 5027 .. 5083 / #cc99b2 / P2A_main sequence
                     Cleavage site after base 5080"
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     CDS             5084..5146
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="This feature has 2 segments:
                        1: 5084 .. 5092 / #cc99b2 / T2A_improves cleavage
                        2: 5093 .. 5146 / #cc99b2 / T2A_main sequence
                     Cleavage site after base 5143"
                     /translation="GSGEGRGSLLTCGDVEENPGP"
     primer_bind     complement(5121..5164)
                     /label=P2AT2A_4SC627_R
                     /note="color: black; sequence: 
                     CTTGCTCACACGCGTcgtTGGGCCAGGATTCTCCTCGACGTCAC; added: 
                     2020-01-08"
     primer_bind     5132..5183
                     /label=mScarlet-I_4SC627_F
                     /note="color: black; sequence: 
                     GAGAATCCTGGCCCAACGacgcgtGTGAGCAAGGGCGAGGCAGTGATCAAGG; 
                     added: 2019-10-25"
     CDS             5156..5848
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="color: #ff0000"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     5214..5233
                     /label=Scar_col_F
                     /note="color: black; sequence: GCTCCATGAACGGCCACGAG"
     primer_bind     5361..5387
                     /label=t74i-Scar_F
                     /note="color: black; sequence: CCAGGGCCTTCATCAAGCACCCCGCCG"
     primer_bind     complement(5361..5387)
                     /label=T74I_scar_R
                     /note="color: black; sequence: CGGCGGGGTGCTTGATGAAGGCCCTGG"
     primer_bind     5520..5542
                     /label=mScar-Seq-F
                     /note="color: black; sequence: TGAAGCTCCGCGGCACCAACTTC; 
                     added: 2019-07-12"
     primer_bind     complement(5635..5654)
                     /label=Scar_col_R
                     /note="color: black; sequence: GGCGCAGGGCCATCTTAATG"
     primer_bind     complement(5823..5870)
                     /label=mScarlet-I_4SC627_R
                     /note="color: black; sequence: 
                     gcttgctggacataccggctccCTTGTACAGCTCGTCCATGCCgccgg; added: 
                     2019-10-25"
     primer_bind     5828..5873
                     /label=myl9_4SC627_F
                     /note="color: black; sequence: 
                     GGCATGGACGAGCTGTACAAGggagccggtatgtccagcaagcggg; added: 
                     2019-10-30"
     CDS             5858..6376
                     /codon_start=1
                     /label=MYL9
                     /note="/vntifkey=4"
                     /note="color: #33cccc"
                     /translation="MSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNR
                     DGFIDKEDLHDMLASLGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIR
                     NAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRIL
                     KHGAKDKDD"
     primer_bind     5863..5880
                     /label=MYL9_col_F
                     /note="color: black; sequence: cagcaagcgggccaaagc; added: 
                     2019-05-04"
     primer_bind     complement(6339..6397)
                     /label=Myl9_4SC627_R
                     /note="color: black; sequence: 
                     ccgcggcccgACatgcatCGCTTAGTCGTCTTTATCCTTGGCGCCATGTTTGAGGatgc
                     ; added: 2019-10-25"
     primer_bind     complement(6344..6368)
                     /label=MYL9_Col_R
                     /note="color: black; sequence: ctttatccttggcgccatgtttgag; 
                     added: 2019-05-04"
     primer_bind     6386..6401
                     /label=SC452vect_F
                     /note="color: black; sequence: GAATTCACGCGTcgggccgcggtaac; 
                     added: 2019-05-04"
     misc_feature    6391..7019
                     /label=IRES
                     /note="color: #339966"
     primer_bind     complement(6436..6455)
                     /label=PHGrp1_colony.REV
                     /note="color: black; sequence: cggaattgatcccgctagag"
     primer_bind     complement(6541..6560)
                     /label=IRES_5'-Seq_R
                     /note="color: black; sequence: CCTCACATTGCCAAAAGACG"
     primer_bind     complement(6586..6608)
                     /label=IRES_4SEQ
                     /note="color: black; sequence: AAGACCCCTAGGAATGCTCGTCA; 
                     added: 2020-01-03"
     CDS             7020..7448
                     /codon_start=1
                     /label=BlastR
                     /note="color: #ff9966"
                     /translation="MVMKTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEI
                     ISAVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGM
                     CRELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN"
     primer_bind     complement(7421..7468)
                     /label=BlastR_SC417.REV
                     /note="color: black; sequence: 
                     ccagacgcgccccgcccgggTTAATTTCGGGTATATTTGAGTGGAATG"
     misc_feature    7472..8063
                     /label=WPRE
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    7472..8060
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(7943..7954)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 7942"
                     /translation="IEGR"
     LTR             8268..8901
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     complement(9030..9046)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    9054..9070
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(9078..9108)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 9078 .. 9084 / #ffffff / -10
                        2: 9085 .. 9102 / #ffffff
                        3: 9103 .. 9108 / #ffffff / -35"
     protein_bind    9123..9144
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(9432..10017)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(10188..11048)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10188 .. 10979 / #ccffcc
                        2: 10980 .. 11048 / #ccffcc / signal sequence
                     Cleavage site after base 10979"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10556..10583)
                     /label=BB_overlap_R
                     /note="color: black; sequence: 
                     CATGTAACTCGCCTTGATCGTTGGGAAC; added: 2019-07-19"
     primer_bind     10556..10583
                     /label=BB_overlap_F
                     /note="color: black; sequence: 
                     GTTCCCAACGATCAAGGCGAGTTACATG; added: 2019-07-19"
     promoter        complement(11049..11153)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    11201..11335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cacatatgcg ggaggtggtc
     2221 cctgcagtta cgccaatgat aacccccgcc agaaaaatct tagtagcctt ccctttttgt
     2281 tttccgtgcc ccaactcggc ggattgactc ggccccttcc ggaaacaccc gaatcaactt
     2341 ctagtcaaat tattgttcac gccgcaatga cccacccctg gcccgcgtct gtggaactga
     2401 cccctggtgt acaggagagt tcgctgctga aagtggtccc aaaggggtac tagtttttaa
     2461 gctcccaact ccccctcccc cagcgtctgg aggattccac accctcgcac cgcaggggcg
     2521 aggaagtggg cggagtccgg ttttggcgcc agccgctgag gctgccaagc agaaaagcca
     2581 ccgctgagga gactccggtc actgtcctcg ccccgcctcc cccttccctc cccttgggga
     2641 ccaccgggcg ccacgccgcg aacggtaagt gccgcggtcg tcggcgcctc cgccctcccc
     2701 ctagggcccc aattcccagc gggcgcggcg cgcggcccct ccccccgccg ggcgcgcgcc
     2761 cgctgccccg cccttcgtgg ccgcccggcg tgggcggtgc cacccctccc cccggcggcc
     2821 cccctgcagc cgcgcgcagc tcccggctcc ctcccccttc ggatgtggct tgagctgtag
     2881 gcgcggagac tcgaggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     2941 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     3001 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     3061 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     3121 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     3181 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
     3241 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
     3301 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
     3361 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
     3421 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
     3481 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
     3541 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
     3601 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
     3661 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     3721 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     3781 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     3841 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     3901 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     3961 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     4021 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga gcgctatggc
     4081 gcgtaaggtc gatctcacct cctgcgatcg cgagccgatc cacatccccg gcagcattca
     4141 gccgtgcggc tgcctgctag cctgcgacgc gcaggcggtg cggatcacgc gcattacgga
     4201 aaatgccggc gcgttctttg gacgcgaaac tccgcgggtc ggtgagctac tcgccgatta
     4261 cttcggcgag accgaagccc atgcgctgcg caacgcactg gcgcagtcct ccgatccaaa
     4321 gcgaccggcg ctgatcttcg gttggcgcga cggcctgacc ggccgcacct tcgacatctc
     4381 actgcatcgc catgacggta catcgatcat cgagttcgag cctgcggcgg ccgaacaggc
     4441 cgacaatccg ctgcggctga cgcggcagat catcgcgcgc accaaagaac tgaagtcgct
     4501 cgaagagatg gccgcacggg tgccgcgcta tctgcaggcg atgctcggct atcaccgcgt
     4561 gatgttgtac cgcttcgcgg acgacggctc cgggatggtg atcggcgagg cgaagcgcag
     4621 cgacctcgag agctttctcg gtcagcactt tccggcgtcg ctggtcccgc agcaggcgcg
     4681 gctactgtac ttgaagaacg cgatccgcgt ggtctcggat tcgcgcggca tcagcagccg
     4741 gatcgtgccc gagcacgacg cctccggcgc cgcgctcgat ctgtcgttcg cgcacctgcg
     4801 cagcatctcg ccctgccatc tcgaatttct gcggaacatg ggcgtcagcg cctcgatgtc
     4861 gctgtcgatc atcattgacg gcacgctatg gggattgatc atctgtcatc attacgagcc
     4921 gcgtgccgtg ccgatggcgc agcgcgtcgc ggccgaaatg ttcgccgact tcttatcgct
     4981 gcacttcacc gccgcccacc accaacgcgg aagtgcaggc agcggcgcca cgaacttctc
     5041 tctgttaaag caagcaggag acgtggaaga aaatcccggt cctggctccg gcgagggcag
     5101 aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggcccaacga cgcgtgtgag
     5161 caagggcgag gcagtgatca aggagttcat gcggttcaag gtgcacatgg agggctccat
     5221 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     5281 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttctcctggg acatcctgtc
     5341 ccctcagttc atgtacggct ccagggcctt catcaagcac cccgccgaca tccccgacta
     5401 ctataagcag tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     5461 cggcgccgtg accgtgaccc aggacacctc cctggaggac ggcaccctga tctacaaggt
     5521 gaagctccgc ggcaccaact tccctcctga cggccccgta atgcagaaga agacaatggg
     5581 ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc gtgctgaagg gcgacattaa
     5641 gatggccctg cgcctgaagg acggcggccg ctacctggcg gacttcaaga ccacctacaa
     5701 ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtc gaccgcaagt tggacatcac
     5761 ctcccacaac gaggactaca ccgtggtgga acagtacgaa cgctccgagg gccgccactc
     5821 caccggcggc atggacgagc tgtacaaggg agccggtatg tccagcaagc gggccaaagc
     5881 caagaccacc aagaagcggc cacagcgggc cacatccaat gtcttcgcaa tgtttgacca
     5941 gtcccagatc caggagttta aggaggcttt caacatgatt gaccagaacc gtgatggctt
     6001 cattgacaag gaggacctgc acgacatgct ggcctcgctg gggaagaacc ccacagacga
     6061 atacctggag ggcatgatga gcgaggcccc ggggcccatc aacttcacca tgttcctcac
     6121 catgtttggg gagaagctga acggcacgga ccccgaggat gtgattcgca acgcctttgc
     6181 ctgcttcgac gaggaagcct caggtttcat ccatgaggac cacctccggg agctgctcac
     6241 caccatgggt gaccgcttca cagatgagga agtggacgag atgtaccggg aggcacccat
     6301 tgataagaaa ggcaacttca actacgtgga gttcacccgc atcctcaaac atggcgccaa
     6361 ggataaagac gactaagcga tgcatgtcgg gccgcggtaa caattgttaa ctaacttaag
     6421 ctagcaacgg tttccctcta gcgggatcaa ttccgccccc cccccctaac gttactggcc
     6481 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     6541 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     6601 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     6661 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     6721 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     6781 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     6841 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     6901 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     6961 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gataatacca
     7021 tggtcatgaa aacatttaac atttctcaac aagatctaga attagtagaa gtagcgacag
     7081 agaagattac aatgctttat gaggataata aacatcatgt gggagcggca attcgtacga
     7141 aaacaggaga aatcatttcg gcagtacata ttgaagcgta tataggacga gtaactgttt
     7201 gtgcagaagc cattgcgatt ggtagtgcag tttcgaatgg acaaaaggat tttgacacga
     7261 ttgtagctgt tagacaccct tattctgacg aagtagatag aagtattcga gtggtaagtc
     7321 cttgtggtat gtgtagggag ttgatttcag actatgcacc agattgtttt gtgttaatag
     7381 aaatgaatgg caagttagtc aaaactacga ttgaagaact cattccactc aaatataccc
     7441 gaaattaacc cgggcggggc gcgtctggaa caatcaacct ctggattaca aaatttgtga
     7501 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     7561 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     7621 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     7681 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     7741 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     7801 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     7861 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     7921 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     7981 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     8041 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     8101 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     8161 gaggaggagg aggtgggttt ttccagtcac acctcaggta cctttaagac caatgactta
     8221 caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat
     8281 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     8341 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
     8401 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
     8461 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
     8521 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
     8581 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
     8641 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
     8701 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8761 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8821 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8881 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     8941 ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg
     9001 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     9061 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     9121 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     9181 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     9241 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     9301 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     9361 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9421 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9481 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9541 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9601 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9661 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9721 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9781 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9841 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9901 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9961 ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    10021 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    10081 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    10141 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    10201 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    10261 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    10321 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    10381 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    10441 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    10501 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    10561 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10621 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10681 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10741 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10801 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10861 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10921 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10981 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    11041 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    11101 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    11161 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta
    11221 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    11281 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    11341 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    11401 caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    11461 aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa gaaattgtat
    11521 ttgttaaata tgtactacaa acttagtagt
//
