LOCUS       Exported                9070 bp ds-DNA     circular SYN 10-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pGJK_His-SUMO-LbuCas13a
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Doudna Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 10, 2021 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(560..1375)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1497..2085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2271..2413
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2515..2706)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3515..4597)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(4598..4675)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        4984..5002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5042..5064
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5071..5100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="MGSSHHHHHH"
     CDS             5110..5127
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5131..5427
                     /codon_start=1
                     /label=bdSUMO
                     /translation="MSGSAAGGEEDKKPAGGEGGGAHINLKVKGQDGNEVFFRIKRSTQ
                     LKKLMNAYCDRQSVDMTAIAFLFDGRRLRAEQTPDELEMEDGDEIDAMLHQTGG"
     CDS             5428..8904
                     /codon_start=1
                     /label=LbuCas13a
                     /translation="MKVTKVGGISHKKYTSEGRLVKSESEENRTDERLSALLNMRLDMY
                     IKNPSSTETKENQKRIGKLKKFFSNKMVYLKDNTLSLKNGKKENIDREYSETDILESDV
                     RDKKNFAVLKKIYLNENVNSEELEVFRNDIKKKLNKINSLKYSFEKNKANYQKINENNI
                     EKVEGKSKRNIIYDYYRESAKRDAYVSNVKEAFDKLYKEEDIAKLVLEIENLTKLEKYK
                     IREFYHEIIGRKNDKENFAKIIYEEIQNVNNMKELIEKVPDMSELKKSQVFYKYYLDKE
                     ELNDKNIKYAFCHFVEIEMSQLLKNYVYKRLSNISNDKIKRIFEYQNLKKLIENKLLNK
                     LDTYVRNCGKYNYYLQDGEIATSDFIARNRQNEAFLRNIIGVSSVAYFSLRNILETENE
                     NDITGRMRGKTVKNNKGEEKYVSGEVDKIYNENKKNEVKENLKMFYSYDFNMDNKNEIE
                     DFFANIDEAISSIRHGIVHFNLELEGKDIFAFKNIAPSEISKKMFQNEINEKKLKLKIF
                     RQLNSANVFRYLEKYKILNYLKRTRFEFVNKNIPFVPSFTKLYSRIDDLKNSLGIYWKT
                     PKTNDDNKTKEIIDAQIYLLKNIYYGEFLNYFMSNNGNFFEISKEIIELNKNDKRNLKT
                     GFYKLQKFEDIQEKIPKEYLANIQSLYMINAGNQDEEEKDTYIDFIQKIFLKGFMTYLA
                     NNGRLSLIYIGSDEETNTSLAEKKQEFDKFLKKYEQNNNIKIPYEINEFLREIKLGNIL
                     KYTERLNMFYLILKLLNHKELTNLKGSLEKYQSANKEEAFSDQLELINLLNLDNNRVTE
                     DFELEADEIGKFLDFNGNKVKDNKELKKFDTNKIYFDGENIIKHRAFYNIKKYGMLNLL
                     EKIADKAGYKISIEELKKYSNKKNEIEKNHKMQENLHRKYARPRKDEKFTDEDYESYKQ
                     AIENIEEYTHLKNKVEFNELNLLQGLLLRILHRLVGYTSIWERDLRFRLKGEFPENQYI
                     EEIFNFENKKNVKYKGGQIVEKYIKFYKELHQNDEVKINKYSSANIKVLKQEKKDLYIR
                     NYIAHFNYIPHAEISLLEVLENLRKLLSYDRKLKNAVMKSVVDILKEYGFVATFKIGAD
                     KKIGIQTLESEKIVHLKNLKKKKLMTDRNSEELCKLVKIMFEYKMEEKKSEN"
     CDS             8914..8931
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      8998..9045
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     5041 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
     5101 agcagcggcc tggtgccgcg cggcagccat atgtcaggta gtgcagctgg gggagaagag
     5161 gacaaaaaac cggcaggtgg cgagggtggc ggtgcacata ttaatttaaa ggtgaaaggt
     5221 caggatggta atgaagtctt cttccgaatc aaacgcagca cccagctgaa aaaactgatg
     5281 aatgcgtact gcgatcgtca gtccgttgat atgaccgcaa ttgcgttctt gttcgacggc
     5341 cgccgtctgc gtgctgaaca gactccggat gaattggaga tggaagacgg cgatgagatc
     5401 gacgcaatgc tgcaccagac cggtggcatg aaagtcacca aagtgggtgg tatctcccat
     5461 aaaaaataca cctctgaggg tcgtctggtt aagagtgagt ccgaggaaaa ccgtacggat
     5521 gagcgcctgt ccgctctgtt aaatatgcgt ctggatatgt atatcaagaa cccgagctcc
     5581 accgaaacga aagaaaatca gaaacgtatt ggcaagctga aaaaattctt ctctaacaag
     5641 atggtatatt tgaaggataa taccctgagc ctgaagaatg gtaagaagga gaacatagac
     5701 cgcgaatact cagagacaga tatcctggaa tcagacgtgc gtgataagaa gaacttcgcc
     5761 gttctgaaga aaatctatct aaacgaaaac gttaattccg aagaattgga ggtctttcgt
     5821 aacgacatca aaaagaagct caacaaaatc aactcgctga agtacagctt tgaaaagaat
     5881 aaggcgaatt atcaaaaaat caacgaaaac aacatcgaaa aggtggaggg caaatcgaaa
     5941 cgcaacatca tttacgacta ttatcgtgaa agcgcgaaac gtgatgcgta cgttagcaat
     6001 gtgaaagaag ccttcgacaa attatataaa gaggaggata tcgctaaatt agttctggag
     6061 atcgaaaacc tgaccaaatt ggagaaatac aagattcgtg aattttacca tgaaattatc
     6121 ggccgtaaga acgataagga gaattttgcg aaaatcatct atgaggagat tcagaatgtt
     6181 aataacatga aagaactcat cgaaaaggtt ccggatatgt ctgagctgaa gaaatctcag
     6241 gttttttaca agtactacct ggacaaagaa gagcttaatg acaagaacat caaatatgca
     6301 ttctgccatt ttgttgaaat cgaaatgagc cagctgctga agaattacgt gtataagaga
     6361 ctctctaaca ttagcaacga caaaatcaag cgtattttcg agtatcaaaa cttgaagaag
     6421 ctgatagaaa acaagctgct caacaaactg gacacctatg tgcgtaattg tggtaaatac
     6481 aattactatc tgcaagacgg tgagattgcg accagcgact tcatcgcgcg taaccgccaa
     6541 aacgaggcgt ttctgcgtaa tatcatcgga gttagcagcg ttgcatattt cagcctacgc
     6601 aacatcctgg aaaccgaaaa cgagaatgat atcaccggtc gcatgcgtgg taagaccgtt
     6661 aaaaataaca agggcgagga aaagtacgtg agcggtgaag tggataaaat ttataacgag
     6721 aataaaaaga atgaggtgaa agaaaacctg aagatgtttt acagctatga cttcaacatg
     6781 gataataaaa acgaaattga agatttcttc gccaacatcg atgaggccat ttcctctatt
     6841 cgtcatggta tagtacactt caacctggag ctggaaggca aggacatctt tgccttcaaa
     6901 aacatcgctc cgagcgaaat ctccaaaaaa atgtttcaga acgagatcaa tgagaaaaag
     6961 ttgaagctga aaatctttcg ccaactgaat tctgcgaatg tcttccgtta tctggagaag
     7021 tacaagattc tgaattactt gaaacgcacc cgttttgagt tcgtcaacaa gaacatccca
     7081 tttgtgccgt cgttcaccaa attgtacagc cgtattgacg acctgaaaaa ctccctgggc
     7141 atctactgga aaaccccgaa aaccaatgac gataacaaga ctaaagagat catcgatgct
     7201 cagatttacc tgctgaagaa catatattat ggcgagttcc ttaactattt catgagcaat
     7261 aacggcaatt ttttcgagat ttctaaagag attattgaac tgaacaagaa tgataagcgc
     7321 aacttgaaga ccggctttta taagctacaa aaatttgagg acattcaaga aaaaattccg
     7381 aaagaatact tggcgaacat ccagagtctg tacatgatta atgcaggcaa tcaggatgag
     7441 gaggaaaaag acacctatat cgactttatt caaaaaattt tcctgaaagg cttcatgacg
     7501 tacttagcga ataatggtcg ccttagcctg atttacatcg gctccgatga ggaaacgaat
     7561 accagtctgg ctgaaaagaa gcaggagttc gataaatttt tgaagaaata tgaacaaaac
     7621 aacaacatta agattccgta tgaaatcaac gagtttctga gggaaatcaa acttggtaac
     7681 attttgaagt acactgagcg cctgaacatg ttttacctga ttctgaagtt gcttaaccac
     7741 aaagagttga ccaatctgaa aggcagcctg gagaagtacc aatcggcgaa caaggaagag
     7801 gcgttcagcg accagctgga gttgatcaac ctgctgaacc tggacaacaa ccgtgttacc
     7861 gaagactttg agctcgaagc ggatgaaatt ggtaaattcc tggactttaa tggtaacaag
     7921 gtgaaggaca acaaagagct gaagaagttt gataccaaca aaatctactt cgacggcgaa
     7981 aacatcatca agcaccgtgc cttctacaac attaaaaagt acggtatgct gaatctgctg
     8041 gaaaaaatcg ccgataaggc gggttataaa atctcgatcg aagagttgaa aaaatatagc
     8101 aacaagaaga atgagattga gaaaaaccac aaaatgcagg agaacttgca ccgcaagtac
     8161 gctcgccctc gtaaggacga aaaatttacc gacgaggact acgagagcta caagcaggca
     8221 attgagaaca ttgaggagta tacgcatctc aaaaacaagg ttgaattcaa cgagttaaat
     8281 ctgctgcaag gtctgttgct gcgcattctt caccgtttag ttggttatac gagcatttgg
     8341 gaaagagacc tgcgttttcg tctgaagggt gagttcccgg aaaaccagta tattgaagaa
     8401 atttttaatt tcgagaacaa gaaaaacgta aaatacaagg gtggccaaat tgttgagaag
     8461 tacatcaagt tctataagga gcttcaccaa aacgacgagg tgaaaataaa caaatactcc
     8521 tctgcgaata ttaaagtgct gaagcaagaa aagaaagatc tgtacattcg caactacatt
     8581 gcccacttta actatatccc gcatgcggaa atttccctgt tggaagtgct ggaaaacctg
     8641 cgtaagctgc tgagctacga tcgtaaactc aagaatgcgg ttatgaagtc cgtggtggac
     8701 attctgaagg agtatggttt tgtagcgacc tttaaaatcg gcgcggataa gaaaatcggc
     8761 attcaaaccc tggaatccga aaaaattgtc cacctgaaga acctgaagaa gaagaaactg
     8821 atgacggatc gtaatagcga ggagctttgc aaactggtga aaatcatgtt cgagtacaaa
     8881 atggaagaga agaaaagcga gaattaactc gagcaccacc accaccacca ctgagatccg
     8941 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     9001 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
     9061 atatccggat
//
