LOCUS       pDF0250_DisCas7-11_ful 13705 bp ds-DNA     circular     11-JUN-2021
DEFINITION  .
SOURCE      Cloning vector pACYC184                                            
  ORGANISM  Cloning vector pACYC184 other sequences; artificial sequences;     
            vectors.
FEATURES             Location/Qualifiers
     misc_feature    23..568
                     /label="ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          27..42
                     /label="PG0390"
                     /note="sequence: CATAGGCTCCGCCCCC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(27..42)
                     /label="PG0397"
                     /note="sequence: gggggcggagcctatg"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          452..478
                     /label="PG0184"
                     /note="sequence: agccagttacctcggttcaaagagttg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    604..791
                     /label="Golden gate insert piece"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    605..639
                     /label="synthetic promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    640..674
                     /label="DR"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     source          640..727
                     /label="type strain of Desulfonema ishimotonii"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    696..730
                     /label="Final DR (end 3 bases differ)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          718..762
                     /label="FP0107"
                     /note="sequence: gatgtcacggttgccacccGGATCCcatgtgcgctgtaccggtgg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     source          743..12315
                     /label="type strain of Desulfonema ishimotonii"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    770..791
                     /label="Primer 3"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    775..777
                     /label="misc_feature"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     source          775..777
                     /label="type strain of Desulfonema ishimotonii"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    778..12315
                     /label="misc_feature"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1320..1982
                     /label="RpoE"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             1320..1982
                     /label="DENIS_1078"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     gene            1320..1982
                     /label="DENIS_1078"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             1979..3229
                     /label="DENIS_1079"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     gene            1979..3229
                     /label="DENIS_1079"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2635..2666
                     /label="Primer 4"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2672..3229
                     /label="Translation 2672-3229"
     gene            3238..4935
                     /label="DENIS_1080"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             3238..4935
                     /label="DENIS_1080"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3238..4935
                     /label="DUF4384"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             3238..4935
                     /label="Translation 3238-4935"
     CDS             4937..7192
                     /label="DENIS_1081"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     gene            4937..7192
                     /label="DENIS_1081"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    4937..7192
                     /label="TPR-CHAT"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             4937..7192
                     /label="Translation 4937-7192"
     misc_feature    6608..7036
                     /label="CHAT domain"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    6779..6781
                     /label="catalytic H"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    6908..6910
                     /label="catalytic residue"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          7174..7200
                     /label="FP0095"
                     /note="sequence: cggcttcccggcggaatgatataaccc"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(7174..7200)
                     /label="FP0096"
                     /note="sequence: gggttatatcattccgccgggaagccg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     repeat_region   7302..7329
                     /label="repeat_region"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(7340..7364)
                     /label="PDG0035"
                     /note="sequence: tatgtgtctcctcggtatgtcgcgt"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    7367..12172
                     /label=" CRISPR-associated RAMP family protein CDS"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             7367..12172
                     /label="DENIS_1082"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     gene            7367..12172
                     /label="DENIS_1082"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             7367..12172
                     /label="Translation 7367-12172"
     repeat_region   8990..9015
                     /label="repeat_region"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    10220..10283
                     /label="Primer 1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          11284..11308
                     /label="FP0152"
                     /note="sequence: agccgatctccgcccctgtcacggt"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(11284..11308)
                     /label="FP0151"
                     /note="sequence: accgtgacaggggcggagatcggct"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    12014..12036
                     /label="Primer 2"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     repeat_region   12233..12258
                     /label="repeat_region"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     repeat_region   12237..12265
                     /label="repeat_region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     repeat_region   12280..12315
                     /label="repeat_region"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     repeat_region   12281..12315
                     /label="repeat_region"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     repeat_region   12282..12315
                     /label="repeat_region"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     repeat_region   12283..12315
                     /label="repeat_region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     repeat_region   12283..12315
                     /label="repeat_region"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    12292..12313
                     /label="Reverse primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    13458..13474
                     /label="A"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    join(13703..13705,1..13)
                     /label="B"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa
       61 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      121 cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
      181 cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt
      241 cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc
      301 ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc
      361 actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga
      421 aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt
      481 agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga
      541 gattacgcgc agaccaaaac gatctcaaga agatcatctt attaatcaga taaaatattt
      601 ctagttgacg gctagctcag tcctaggtac agtgctagcg gttggaaagc cggttttctt
      661 tgatgtcacg gaaccgagac ctactgttgg tctcgggttg gaaagccggt tttctttgat
      721 gtcacggttg ccacccGGAT CCCATGTGCG CTGTACCGGT GGTTCTCCGA CTGAtcaaag
      781 accgggcagc cccggatgaa ttttacatcc ggggctgttc cgcaggcaaa atgccagaga
      841 attctgagca ccggaatctg acgacataac aaaaggcaaa gttgactgaa agaacaggaa
      901 cattatctga aatttttaca aaaaggaggc agatatggtg acacccggca tgattcagag
      961 cgcaggcaaa gagatgggac ttttcatccc gtgggatgtg gcattgggca tggttctctg
     1021 cgttgccgga aaaatactca tgggcgagag cctggaaaat gcggttttgt cgtgttggaa
     1081 ataaagccca ttttaaaatg tcgcggataa gatgaataat accaaatcgc agatggaata
     1141 tgtcagccgg agcaaggctc cggcaatccg cactccgaac aaaagataaa ttcaatacag
     1201 aatcggtaga agcagggatt ttttttattg acgctgataa caggaaaaag ctacaaacgt
     1261 agtttgcaga tttttatcat tacctacaac tttttttaaa atcaggatat tcgaaaatta
     1321 tgagtttaaa tcatccctgt ttgtctgccc gtgctgcgtc acccgaatgt actgtttgtc
     1381 ctgtttttaa ggctgggcat caggaggcat ttgtcaaaat tcttgaaaaa aaggttcggt
     1441 ttctcgaaag ccgaaaacat ctgtcaccgg aagacaagga agatattctc agcgaatttg
     1501 ttctgcaaat ccagtcttcc acatcctcat ttaagggaca gaatgcaaaa tttgaaacct
     1561 gggcctatag aatatttcaa aatgtatggg ctgatttttt tagaaaaatc aaaaatttca
     1621 aaacgcagag aaccgatgac ggcatttata ttcaggaaac aataaaagta aaaaaaacgg
     1681 ataaagacgg aagagaaatg cttgaggaaa aagcaaggac gggcgtattt cttccatata
     1741 aagagaatat cgaagacaga attgaatcgc gggaaatttt ttcaattttc cgcaaagcag
     1801 taaaagatcc gaaaaaggca ttttgctatc atttgcttgt taatcattat acttggaaaa
     1861 gcgaagggtt gaaacaggat gaaatggcgg ctcggtatga aatgaggcac gataccttca
     1921 gaaaacgcct ttcagaatgc cgctcaagat tcaaacaata tctgaaagag gttacgcaat
     1981 gacaattccc gaaatctgcc gcgagccagg atatgttttt tacgaaggaa tttttgaaag
     2041 tgttctttcg cgtgactgtt attgctttaa agtggagaac gaggattaca gcggagaggt
     2101 gataatggaa aaacattatt cccccgacaa tgtgcagcta aaaagcggta aacctgtcac
     2161 tgaccttgtt gttgttagat atgagaaaaa ccgtctgttt gtggccatga ataattattt
     2221 tgatgatctg atggggcatt ttttcaatac ggacagagga gtttttgtaa aaaatattcc
     2281 ttttccaaac tgggaaaggg cttgggaaac agcgtttgcc catacaaatt atcagcctga
     2341 aaagttgcaa ctcaagaaga gagaacacag aaacgaacga tggattgagg taaaaagtcc
     2401 ccggtgtcgg atacctgccc atgaacattc tcatatcagt gatatggacg gggaaccagt
     2461 gttttttcct gcctcatttt acaatataca aaacagagac aggaacggca ggttgattcc
     2521 ccgcgcactg tataaaaccg aaggcatgga ggagatgccc tgttacttca aaggggaatt
     2581 cagcccggaa tccggcaaca cccggacagt ccacatttat ctgcatggca taacgtttcc
     2641 ttccaaatgg cattatagcg tattgaagca catgatctgt cttccgaaat tcaaaggcaa
     2701 ggatgacaat cgcgacctgt ggaaagcatt tgatggagaa gaaccgcttg aaccagacgt
     2761 agaaattgtg caagcgtgta aactgaaatg caaagattca tgtgatgaaa ggcattgcca
     2821 aaaggaaaaa attttcaaga atgttcacaa aaagatcaga acagcgtgtg aacggtatct
     2881 gagacgggcc atcatatttt ttccccaaca taccgacaaa gctctgagga tttggcaaga
     2941 caggaagagc cagtgcggaa aacccaaatt tattgaggtg gattgtctga ataaaatcga
     3001 aaacaacata ttcagacaag ctctcgggca ggaagagttg gagcggaatg aaattctcat
     3061 cctccgggga tattcatatg actgggattc cgccgaggaa ccaacgattc atctgagtat
     3121 tgtcacaatg tatgcaggcc ccttaaaagg gaaaatgccc cgtgaaaacg cattcaaaaa
     3181 agatttcagg ctgaccggaa acggcaaact ttccatatcg gagaattaag agacaaaatg
     3241 aatacaacaa catacaacac cacaacagac gcgcttttgg aatggggcaa ggtatatttt
     3301 cagaaagagg atttttctga attccttgat aatctcgaag cgtatatttc agatgccggg
     3361 gacagtctga aagatgaact tgagagcggg gtggaaaagc ttgttttggg cataaaatcg
     3421 gcggaagcag ttattttcgg agaggctgtg atcgggacta cgccggaaaa tgaagcatgg
     3481 tacgatgccg aagaatcctt tctgactttg gattgtgccg tatggctgtc ccaagcgctt
     3541 gatcgcgttg ttcgcaggca ggatgcgtca ttggccgaca gcctgatcgc ccgcttggac
     3601 gaagccataa accgcgtcgc cgaaaaactg tatgcggaca atttatctcc gcttcgtttt
     3661 tcatcgctca atgaaatccg tcgaagcgct ctggaggcaa cggatgagaa atatcattat
     3721 ctgtttccct ggcacggggc cgcatgtgat gttgatgaaa acattctgct gattctgacg
     3781 gaggaatatc atctgatcgg ggcagataaa gccggagcca atctttcaga ggaactgcgg
     3841 ggcgacctgc cttttatttt tgccgagttg gagcgggatg aggtgctgcg ggcatatgtt
     3901 gaaaaggaga acgccctttc tcttgctttg gaaaatacca tgcgggaaca ctgggcattc
     3961 ggcctgctgg aagcggcccg tgatgaaggg tacaaccatc cctatcccgc agatgtcggg
     4021 atgaggattc atcaggttgc ccgtgccgta ttttcccaga caaacctctc tccggcggaa
     4081 cgccttgctg ttgccatagc cggggcctgt tttacgcctg agatcagcga agaccggcgg
     4141 cttgagattc tgctggactg tgaggagcgg gtgtgtgaaa tcgaagcccc gaccggagac
     4201 gatacatccg tccgcgttat taaagacttg aaagcgctgg ccgatcaccg tgtccgccat
     4261 gaaatcccgg cagaaagtct tgtcagtctg tggtttgagc agattgaggc ggcggggacg
     4321 gattttgata caaagacacc gatggatgaa ttggtgttgc gaatgctttc cgataacgtc
     4381 atcactctgt ctgttgaccg aaaagcggct tctcagacag aaacagatga tgtgaaaccg
     4441 cagaaaggaa aaatcatacc ctttcctgtt cctgatattg ccaacgatga agttgaatac
     4501 caaaaagctg tgggaatgaa gaaggataaa aaggcggcta atgacagcaa agtcaaattt
     4561 cccggcctgc ttgaaatcca gggttgtcgt gacggggaca aggctatttt gctggaagac
     4621 acagacgatg cggcagctaa tcaccggaaa ctgttttcca ttctgaaagc aggtaaattg
     4681 aattcagctt ttttcattca aagcgatgat ggagaatggg ttgagtcgga atccaaaccg
     4741 acgatggaag ataaccgtat catattacat gacagccatc attccagctt tgtatggata
     4801 ttggataccg gttccatgca gctcagacag agtgtaaagt gtgtcaaaga cgcattgaat
     4861 aagaagacag ggagtgcgaa gaaactgaaa ccgaaaacaa tgatcgtttg ggtcacgatt
     4921 ccgcaggagg gatgatatgt caaatccgat tcgggatatt caggacaggc tgaaaactgc
     4981 aaaatttgac aataaggatg atatgatgaa cttggcatcc tcgttgtaca aatatgaaaa
     5041 gcaactgatg gattcttctg aagcaacact ttgtcagcaa gggttgtcca atcggcccaa
     5101 ttcattcagc cagctttccc aattccggga ttccgacatt caaagtaaag caggcggaca
     5161 gacggggaaa ttttggcaga atgaatatga agcctgcaaa aattttcaaa cccataaaga
     5221 gcggcgggaa actctggagc aaatcatccg ctttctccaa aatggcgcag aagaaaaaga
     5281 tgcggatgat ctgcttctga aaacgcttgc ccgcgcctat tttcacaggg ggttgctcta
     5341 caggcccaaa ggcttctctg ttccggccag gaaagtcgaa gcaatgaaaa aggcaatagc
     5401 gtactgtgaa ataattctgg acaaaaatga agaagagtcg gaggcactaa gaatctggct
     5461 ttatgccgca atggaactcc gacggtgcgg tgaggagtat ccggaaaatt tcgctgaaaa
     5521 acttttttat ctcgcaaatg acggtttcat atccgaactg tatgatatca gactttttct
     5581 tgaatatacg gagcgagaag aagataacaa ttttcttgat atgatccttc aggaaaatca
     5641 ggacagggaa cgcctttttg aactatgtct ttacaaggcc agagcgtgtt tccatctaaa
     5701 tcagttgaat gacgtgagaa tatatggaga aagtgcaatt gataacgcgc cgggggcttt
     5761 tgccgatccg ttctgggatg agcttgttga atttatcaga atgcttagaa ataagaaatc
     5821 ggaattatgg aaagaaattg ccataaaagc gtgggacaag tgccgcgaaa aagaaatgaa
     5881 agttggcaac aatatttatc taagctggta ttgggccaga cagcgagagc tttatgacct
     5941 tgcgtttatg gcgcaggacg gtattgaaaa aaaaacgaga attgctgatt ccctgaaatc
     6001 cagaaccaca ctcagaatcc aggaattgaa tgaactcaga aaagatgcac atagaaaaca
     6061 aaataggaga ttagaagaca aactggatag gattattgag caggaaaatg aagccagaga
     6121 tggtgcttat ctaaggagaa atcctccttg ctttacaggc ggaaaaaggg aagaaatacc
     6181 ttttgccaga ctgccccaaa actggattgc cgtccatttc tatctgaacg aactggaatc
     6241 tcacgaaggc ggaaaaggcg gacatgcgct gatttatgat cctcaaaaag ctgaaaaaga
     6301 tcaatggcag gacaaatcct ttgactacaa agaactgcat cggaaatttt tggagtggca
     6361 ggaaaactat attctgaacg aagaagggtc cgccgatttt ctggtaacac tctgtcggga
     6421 aatcgaaaaa gccatgcctt ttctgtttaa gtcagaagtg attcccgaag acaggccggt
     6481 attgtggatt ccgcatggtt tcctgcatcg actccccctt catgcagcca tgaaatcagg
     6541 taataactcc aatattgaaa tattttggga aagacatgcg tctcgatatc ttcctgcatg
     6601 gcatctgttt gatcctgctc catatagtag agaagaaagc tccactcttt taaagaattt
     6661 tgaagaatat gatttccaga atttggaaaa tggagaaatt gaggtttacg ccccctcatc
     6721 tcccaagaaa gtaaaagagg caattaggga aaatccggcc attctgcttt tgctttgtca
     6781 tggtgaagcc gacatgacca atccttttcg ctcctgcctg aagcttaaaa ataaagatat
     6841 gaccatcttt gatctgctga cagtggaaga tgtaaggctc tccggttcac gaattcttct
     6901 gggtgcctgt gagtccgata tggttccacc gttggagttt tctgtggacg aacacctttc
     6961 tgttagcggt gcatttcttt ctcacaaggc gggagagatt gttgcgggtc tatggacagt
     7021 tgattcggaa aaagttgacg aatgctattc gtatttggtg gaggagaaag attttttgag
     7081 aaatttgcag gaatggcaaa tggcagaaac agaaaacttt cggtctgaaa atgatagttc
     7141 tttattttat aaaatcgccc cgttcagaat catcggcttc ccggcggaat gatataaccc
     7201 acacgtcttc cttctttccc gatccagcag agctacaggg aaagcgtata agaacgggaa
     7261 aaattcaggc cggagcggga ctttcctttt ctcacacatt ttccctgtag ttttcccaag
     7321 gctttgaggg acgatttcca cgcgacatac cgaggagaca cataccatga cgacgactat
     7381 gaaaatttcc atcgaatttc tcgaaccctt tcggatgact aaatggcagg aaagtaccag
     7441 acgaaacaaa aacaacaaag aattcgtccg gggtcaggca tttgccagat ggcacagaaa
     7501 caaaaaagac aacacaaagg gccgtcccta tatcaccgga accctgctcc gttcggcagt
     7561 catccgctcg gcggagaacc tgctgacgct ttccgatgga aaaatttcag aaaagacctg
     7621 ttgtccgggg aaattcgaca ctgaagacaa agacagattg ttacaattgc gccagcgttc
     7681 cacgctcagg tggacagata aaaatccctg cccggacaac gccgaaacat attgcccgtt
     7741 ctgtgaactg ctgggccgat ccggtaacga cggaaagaaa gccgaaaaaa aagattggag
     7801 atttcgtatc catttcggca atctcagttt gcctggaaaa ccggactttg acggcccgaa
     7861 ggcaatcgga tcacagcgcg ttctgaaccg ggtcgatttc aaaagcggca aggcccatga
     7921 cttttttaaa gcctatgaag tggatcatac ccgctttccc cggtttgagg gagagatcac
     7981 cattgacaac aaggtgagtg ccgaggcccg aaagcttctg tgcgacagcc tgaaattcac
     8041 agaccggctc tgcggggcct tgtgtgtcat ccggtttgac gagtatactc cggctgccga
     8101 ttccggcaaa cagacggaga atgtgcaggc agagccaaat gccaatctcg ccgagaaaac
     8161 cgccgagcag atcatcagca tcttggatga caacaaaaaa accgaataca cccgccttct
     8221 ggctgatgcc atccgttcgc tccgccggag cagcaagctt gtcgccggtc tgcccaaaga
     8281 tcacgatgga aaggatgatc attatctctg ggacatcgga aagaaaaaaa aagatgaaaa
     8341 cagcgtaaca atccgccaaa tcctgacaac atcggcggat acaaaagaac ttaaaaatgc
     8401 cggaaagtgg cgcgaattct gcgagaagct gggcgaggca ctctacctga aatccaaaga
     8461 catgagcggc ggcttgaaaa taacccggcg aattctggga gacgccgaat ttcacggaaa
     8521 gccggatcgt ctggaaaaat cccgttctgt ttccatcggc tctgtgctga aagagacggt
     8581 tgtctgcgga gaactggtcg caaaaacgcc gtttttcttc ggagccattg atgaggatgc
     8641 aaaacagacc gatcttcagg tgttgctgac gcccgacaac aaataccgcc tgccgcgctc
     8701 cgctgttcgg ggaatcctgc gccgggattt gcagacatac tttgacagcc cgtgcaatgc
     8761 cgaactcggc ggaaggccct gtatgtgcaa aacctgccgg atcatgcgcg gtatcaccgt
     8821 catggatgcc cgcagtgaat acaatgcgcc gccggaaatc cgccaccgca cccgcatcaa
     8881 tccctttacc ggcacggttg ccgagggagc gctgttcaat atggaagtgg caccggaggg
     8941 aattgtgttt cccttccagc ttcgctaccg gggaagcgaa gacggattgc cggatgcact
     9001 gaaaaccgtc ctgaaatggt gggcagaggg ccaggcgttt atgagcggcg cggcatccac
     9061 ggggaaaggg cgattccgta tggaaaacgc caagtatgag acattggatt tgtccgatga
     9121 aaatcaacgg aatgattatc tgaaaaattg gggatggcgg gatgaaaaag gattggagga
     9181 actgaaaaaa cgcctgaatt ctggcttgcc ggaaccgggc aattaccgtg accccaagtg
     9241 gcacgaaatc aacgtcagta tagaaatggc ctcccccttc atcaatggcg acccgatccg
     9301 cgccgccgtg gataaaaggg gtacggatgt ggtcacgttc gtcaaataca aagccgaagg
     9361 tgaagaggcc aaaccagttt gtgcctacaa ggcggagagt ttcaggggcg tgatacgttc
     9421 ggcagttgcg cgaatccata tggaagatgg tgttccgctg accgaactga cccacagcga
     9481 ttgcgaatgc ctgctctgcc agattttcgg cagcgaatat gaggccggga aaatccggtt
     9541 tgaggatctg gtgtttgaat cagatccaga gccggtgaca ttcgaccatg tggccattga
     9601 ccggtttacc ggcggcgcgg ctgacaaaaa gaaatttgac gattctccgc ttccgggcag
     9661 tccggccaga cctctcatgc taaaaggcag cttctggata cggcgggatg ttctggaaga
     9721 tgaggaatat tgcaaagcgc tgggaaaggc gctggcagat gtgaacaacg ggctttatcc
     9781 tctgggcgga aaaagcgcca tcggatatgg gcaggtaaaa agtctgggga ttaaaggaga
     9841 tgataagcgg atttcccgtt tgatgaaccc cgcttttgat gaaacagatg tggctgtgcc
     9901 ggaaaagccg aaaaccgatg ccgaggttcg gattgaggcg gaaaaggttt attatcctca
     9961 ttattttgtc gagcctcata aaaaggtgga gcgtgaagaa aagccttgcg gccatcagaa
    10021 atttcatgaa ggacggctga ccggaaaaat tcgatgcaaa ctgatcacaa agacgccgct
    10081 cattgtaccg gacacctcga atgacgattt tttcaggccc gcagacaaag aagccagaaa
    10141 agaaaaagat gagtatcaca aaagctatgc ctttttcagg cttcacaagc aaattatgat
    10201 tcccggttcc gagctgcggg gcatggtcag ttcggtttac GAAaccgtga ccaactcctg
    10261 ctttcgcatt tttgatGAAa ccaagcggct ttcgtggcgg atggatgcag accatcagaa
    10321 tgtgcttcaa gattttcttc ccggcagagt tacggctgac ggaaagcata tacaaaaatt
    10381 ttctgaaacc gcacgggttc ctttttatga taaaacccag aagcattttg acatattgga
    10441 tgaacaggaa atagcaggcg aaaaacctgt tagaatgtgg gtcaaaagat ttatcaaacg
    10501 tttgtctctg gtcgatcctg ctaaacatcc tcaaaaaaag caggacaaca aatggaagcg
    10561 cagaaaagag ggaatagcca cattcataga acagaaaaac ggcagttatt atttcaatgt
    10621 agttacgaac aatggctgta cgagctttca tttatggcac aaacctgaca actttgacca
    10681 agagaaactt gaaggtatcc agaatggtga gaaattggat tgttgggtga gagattccag
    10741 atatcaaaag gctttccagg aaatacccga aaatgatccg gatggatggg aatgtaaaga
    10801 aggatacctt catgttgtcg gtcccagcaa agtagagttt tccgacaaga aaggtgatgt
    10861 cataaacaat tttcagggaa ccctgccatc tgtaccgaat gattggaaaa cgatccgaac
    10921 caatgatttc aaaaaccgaa aacgcaaaaa tgaacccgta ttttgctgtg aggatgataa
    10981 gggaaactat tatacgatgg caaaatattg tgaaaccttt ttctttgacc tcaaagaaaa
    11041 tgaggagtat gaaattccag aaaaagcccg tattaaatac aaagagctgt tgagagttta
    11101 taacaataat ccccaagcag ttcctgaatc ggtatttcaa tctcgcgtcg caagagaaaa
    11161 tgtcgaaaag cttaaatctg gcgatcttgt ctattttaaa cacaatgaaa aatatgttga
    11221 ggatattgtt cccgtccgca tttcccggac agttgatgat cggatgatcg gcaaacgcat
    11281 gtcagccgat ctccgcccct gtcacggtga ctgggtggaa gacggcgacc tttccgccct
    11341 gaacgcctac ccggaaaagc gccttctcct gcgtcatccc aaaggattgt gcccggcctg
    11401 ccgccttttc ggaaccggtt catacaaggg ccgggttcgg ttcggatttg caagccttga
    11461 aaatgatccc gaatggctga tacccggcaa gaatcccggt gatcccttcc acgggggacc
    11521 ggtaatgctg tcgcttctgg agcgcccccg gcccacatgg tcgatccccg gatcggacaa
    11581 taagttcaaa gtgccgggcc ggaagtttta tgtccatcat catgcatgga aaacgataaa
    11641 agacggcaac catcccacaa ccggaaaggc catagaacaa agccccaaca accggacagt
    11701 tgaagccttg gccgggggaa acagcttttc ttttgaaatc gcttttgaaa atctgaaaga
    11761 atgggaactc ggtttgctga ttcattccct gcaattggaa aaagggttgg cacacaagct
    11821 gggcatggca aaatcaatgg gattcggcag cgttgagatt gatgttgaat ctgtgcgtct
    11881 gaggaaggac tggaaacagt ggagaaacgg aaacagtgaa ataccaaact ggcttgggaa
    11941 aggctttgcc aagctaaagg aatggttcag agatgagctg gattttatag aaaatctgaa
    12001 gaagttgctc tggtttccag aaGGCgacca agcgccgaga gtctgttatc cgatgctgag
    12061 aaaaaaggat gacccgaacg gaaactccgg ctacgaagaa ctgaaagacg gcgaatttaa
    12121 aaaggaagac cgccagaaaa agctcaccac gccttggacc ccttgggcct gatcttctgt
    12181 gccgcctgat actctccctt cgtaagatga ttcagcatga aaagttcatt atttcagccc
    12241 ggaagttcat tttcgggctg aaacctgttg gtttcctccg ataacccgct tcagcgggtt
    12301 tcaataactg agccggaccg ggtcgaattt gctttcgaat ttctgccatt catccgctta
    12361 ttatcactta ttcaggcgta gcAaccaggc gtttaagggc acCAATAACT GCCTTAAAAA
    12421 AATTACGCCC CGCCCTGCCA CTCATCGCAG TACTGTTGTA ATTCATTAAG CATTCTGCCG
    12481 ACATGGAAGC CATCACAAAC GGCATGATGA ACCTGAATCG CCAGCGGCAT CAGCACCTTG
    12541 TCGCCTTGCG TATAATATTT GCCCATGGTG AAAACGGGGG CGAAGAAGTT GTCCATATTG
    12601 GCCACGTTTA AATCAAAACT GGTGAAACTC ACCCAGGGAT TGGCTGAGAC GAAAAACATA
    12661 TTCTCAATAA ACCCTTTAGG GAAATAGGCC AGGTTTTCAC CGTAACACGC CACATCTTGC
    12721 GAATATATGT GTAGAAACTG CCGGAAATCG TCGTGGTATT CACTCCAGAG CGATGAAAAC
    12781 GTTTCAGTTT GCTCATGGAA AACGGTGTAA CAAGGGTGAA CACTATCCCA TATCACCAGC
    12841 TCACCGTCTT TCATTGCCAT ACGGAATTCC GGATGAGCAT TCATCAGGCG GGCAAGAATG
    12901 TGAATAAAGG CCGGATAAAA CTTGTGCTTA TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA
    12961 ATATCCAGCT GAACGGTCTG GTTATAGGTA CATTGAGCAA CTGACTGAAA TGCCTCAAAA
    13021 TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC
    13081 ATTTTAGCTT CCTTAGCTCC TGAAAATCTC GATAACTCAA AAAATACGCC CGGTAGTGAT
    13141 CTTATTTCAT TATGGTGAAA GTTGGAACCT CTTACGTGCC GATCAACGTC TCATTTTCGC
    13201 CAAAAGTTGG CCCAGGGCTT CCCGGTATCA ACAGGGACAC CAGGATTTAT TTATTCTGCG
    13261 AAGTGATCTT CCGTCACAGG TATTTATTCG GCGCAAAGTG CGTCGGGTGA TGCTGCCAAC
    13321 TTACTGATTT AGTGTATGAT GGTGTTTTTG AGGTGCTCCA GTGGCTTCTG TTTCTATCAG
    13381 CTGTCCCTCC TGTTCAGCTA CTGACGGGGT GGTGCGTAAC GGCAAAAGCA CCGCCGGACA
    13441 TCAGCGCTAG CGGAGTTATA CTGGCTTACT ATGTTATACT GGCTTACTAT GTTGGCACTG
    13501 ATGAGGGTGT CAGTGAAGTG CTTCATGTGG CAGGAGAAAA AAGGCTGCAC CGGTGCGTCA
    13561 GCAGAATATG TGATACAGGA TATATTCCGC TTCCTCGCTC ACTGACTCGC TACGCTCGGT
    13621 CGTTCGACTG CGGCGAGCGG AAATGGCTTA CGAACGGGGC GGAGATTTCC TGGAAGATGC
    13681 CAGGAAGATA CTTAACAGGG AAgtg
//