LOCUS       pDF0191human_codon_opt 11522 bp ds-DNA     circular     19-SEP-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(1..16)
                     /label="rop"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     source          complement(1..3333)
                     /label="source"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(1328..2410)
                     /label="lacI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     2372..2391
                     /label="LacI-R"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        complement(2411..2488)
                     /label="lacI promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     2694..2713
                     /label="pBRrevBam"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        2797..2815
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     2797..2816
                     /label="T7"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    complement(2816..2840)
                     /label="lac operator"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     RBS             complement(2855..2877)
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             2884..3333
                     /label="Translation 2884-3333"
     CDS             2896..2913
                     /label="6xHis"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2923..2940
                     /label="thrombin site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             2953..2976
                     /label="Strep-Tag II"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             3013..3036
                     /label="Strep-Tag II"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             3037..3327
                     /label="SUMO"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3334..3357
                     /label="fwd primer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3334..8772
                     /label="human codon opt Cjc"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             3334..8772
                     /label="Translation 3334-8772"
     misc_feature    8747..8772
                     /label="rev primer"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     source          complement(8773..11522)
                     /label="source"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     complement(8819..8837)
                     /label="T7 Term"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      complement(8833..8880)
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        complement(9055..9083)
                     /label="tet promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(9110..9128)
                     /label="pBRforEco"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        9196..9300
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             9301..10161
                     /label="AmpR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(9519..9538)
                     /label="Amp-R"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      10332..10920
                     /label="ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     10821..10840
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     11074..11091
                     /label="L4440"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(11106..11245)
                     /label="bom"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(11171..11190)
                     /label="pRS-marker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     11331..11353
                     /label="pGEX 3'"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(11347..11522)
                     /label="rop"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga
       61 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
      121 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
      181 tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc
      241 agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt
      301 ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg
      361 ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag
      421 taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc
      481 gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg
      541 cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat
      601 tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt
      661 cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc
      721 ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt
      781 gacgatcagc ggtccaatga tcgaagttag gctggtaaga gccgcgagcg atccttgaag
      841 ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc
      901 gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa
      961 cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc
     1021 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
     1081 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
     1141 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
     1201 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     1261 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     1321 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1381 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
     1441 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     1501 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
     1561 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
     1621 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
     1681 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
     1741 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
     1801 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
     1861 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
     1921 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
     1981 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
     2041 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
     2101 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
     2161 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
     2221 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
     2281 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
     2341 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
     2401 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     2461 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
     2521 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     2581 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     2641 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     2701 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     2761 ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat
     2821 tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat
     2881 accatgggca gcagccatca tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc
     2941 catatggcta gctggagcca tccgcagttt gaaaaaggtg gtggtagcgg tggtggttca
     3001 ggtggtagtg catggtcaca ccctcagttt gagaaaatgt cggactcaga agtcaatcaa
     3061 gaagctaagc cagaggtcaa gccagaagtc aagcctgaga ctcacatcaa tttaaaggtg
     3121 tccgatggat cttcagagat cttcttcaag atcaaaaaga ccactccttt aagaaggctg
     3181 atggaagcgt tcgctaaaag acagggtaag gaaatggact ccttaagatt cttgtacgac
     3241 ggtattagaa tccaagctga tcagacccct gaagatttgg acatggagga taacgatatt
     3301 attgaggctc acagagaaca gattggtgga tccATGCACA CCATCCTGCC TATCCACCTG
     3361 ACCTTTCTGG AACCCTACAG ACTGGCCGAG TGGCACGCCA AGGCCGACAG AAAGAAGAAC
     3421 AAGAGATACC TGCGGGGCAT GAGCTTCGCC CAGTGGCACA AGGATAAGGA CGGCATCGGC
     3481 AAGCCCTACA TCACCGGCAC ACTGCTGAGA AGCGCCGTGC TGAATGCCGC CGAGGAACTG
     3541 ATCAGCCTGA ATCAAGGCAT GTGGGCCAAA GAGCCCTGCT GCAACGGCAA GTTCGAGACA
     3601 GAGAAGGACA AGCCTGCCGT GCTGCGGAAG AGGCCTACCA TCCAGTGGAA AACAGGCAGA
     3661 CCCGCCATCT GCGACCCCGA GAAGCAAGAG AAGAAGGACG CCTGTCCTCT GTGCATGCTG
     3721 CTGGGCAGAT TTGACAAGGC CGGCAAGCGG CACCGGGACA ACAAATACGA CAAGCACGAC
     3781 TACGACATCC ACTTCGACAA CCTGAACCTG ATCACCGACA AGAAGTTCTC TCACCCCGAC
     3841 GATATCGCCA GCGAGCGGAT CCTGAACAGA GTGGATTACA CCACCGGCAA GGCCCACGAC
     3901 TACTTCAAAG TGTGGGAAGT CGACGACGAC CAGTGGTGGC AGTTTACCGG CACCATCACC
     3961 ATGCACGACG ACTGCTCTAA GGCCAAGGGA CTGCTGCTGG CCTCTCTGTG CTTCGTGGAT
     4021 AAGCTGTGTG GCGCCCTGTG CCGGATTGAA GTGACCGGCA ACAACAGCCA GGACGAGAAC
     4081 AAAGAGTACG CCCATCCTGA CACAGGCATC ATCACATCCC TGAATCTGAA GTACCAGAAC
     4141 AACTCCACCA TCCACCAGGA CGCCGTGCCA CTGTCTGGAT CTGCCCACGA CAATGACGAG
     4201 CCTCCAGTGC ACGACAACGA TAGCAGCCTG GACAACGACA CAATCACCCT GCTGAGCATG
     4261 AAGGCCAAAG AAATCGTGGG CGCCTTCAGA GAGAGCGGAA AGATCGAGAA GGCCCGGACA
     4321 CTGGCCGATG TGATCAGAGC CATGAGACTG CAGAAGCCCG ACATCTGGGA GAAGCTGCCC
     4381 AAGGGCATCA ACGATAAGCA CCACCTGTGG GATAGAGAAG TGAACGGAAA GAAGCTGCGG
     4441 AACATCCTGG AAGAACTGTG GCGGCTGATG AACAAGAGGA ACGCCTGGCG GACCTTCTGC
     4501 GAGGTGCTGG GCAATGAGCT GTACCGGTGC TACAAAGAGA AAACCGGCGG CATCGTGCTG
     4561 AGATTCAGAA CCCTGGGCGA AACCGAGTAC TACCCCGAGC CTGAAAAGAC CGAGCCTTGC
     4621 CTGATCAGCG ATAACAGCAT CCCCATCACA CCTCTCGGCG GCGTGAAAGA ATGGATCATC
     4681 ATCGGCAGAC TGAAGGCCGA GACACCCTTC TACTTTGGCG TGCAGAGCAG CTTCGACAGC
     4741 ACCCAGGATG ACCTGGACCT GGTGCCTGAC ATCGTGAACA CCGACGAGAA GCTGGAAGCC
     4801 AACGAGCAGA CCAGCTTCAG AATCCTGATG GACAAGAAGG GCCGCTACAG AATCCCTCGG
     4861 AGCCTGATTA GAGGCGTGCT GAGGCGGGAT CTGAGAACAG CCTTTGGCGG CTCTGGCTGC
     4921 ATCGTGGAAC TGGGCAGAAT GATCCCCTGC GACTGCAAAG TGTGCGCCAT CATGCGGAAG
     4981 ATCACCGTGA TGGACAGCAG AAGCGAGAAC ATCGAGCTGC CCGATATCCG GTACAGGATC
     5041 CGGCTGAATC CTTACACCGC CACCGTGGAT GAGGGCGCTC TGTTCGATAT GGAAATCGGC
     5101 CCCGAGGGCA TCACCTTTCC ATTCGTGTTC AGATACAGAG GCGAGGACGC CCTGCCTCGG
     5161 GAACTTTGGA GCGTGATCAG ATACTGGATG GACGGCATGG CCTGGCTCGG CGGATCTGGA
     5221 TCTACTGGCA AGGGAAGATT CGCCCTGATC GACATCAAGG TGTTCGAGTG GGACCTGTGC
     5281 AACGAGGAAG GCCTGAAGGC CTACATCTGC AGCAGAGGCC TGAGAGGCAT CGAGAAAGAG
     5341 GTGCTGCTGG AAAACAAGAC AATCGCCGAG ATCACCAACC TGTTCAAGAC CGAGGAAGTG
     5401 AAGTTCTTCG AGAGCTACAG CAAGCACATC AAGCAGCTGT GCCACGAGTG CATTATCAAC
     5461 CAGATCAGCT TCCTGTGGGG CCTGAGAAGC TACTACGAGT ATCTGGGCCC TCTGTGGACC
     5521 GAAGTGAAAT ACGAGATCAA GATCGCCTCT CCACTGCTGT CCTCCGACAC CATTTCTGCC
     5581 CTGCTGAACA AGGACAACAT CGACTGTATC GCCTACGAGA AGCGCAAGTG GGAGAATGGC
     5641 GGCATCAAGT TCGTGCCCAC CATCAAGGGC GAGACAATCA GAGGAATCGT GCGGATGGCC
     5701 GTGGGCAAGA GATCTGGCGA TCTCGGCATG GACGACCACG AGGATTGCAG CTGTACCCTG
     5761 TGCACCATCT TCGGCAACGA GCACGAGGCC GGAAAGCTGA GATTTGAGGA CCTGGAAGTG
     5821 GTGGAAGAGA AACTGCCCTC CGAGCAGAAC AGCGACAGCA ACAAGATCCC TTTCGGCCCC
     5881 GTTCAGGACG GCGACGGCAA CAGAGAAAAA GAATGCGTGA CCGCCGTGAA GTCCTACAAG
     5941 AAGAAACTGA TCGACCACGT GGCCATCGAC CGGTTTCATG GCGGAGCCGA GGACAAGATG
     6001 AAGTTCAACA CACTGCCTCT GGCCGGCAGC TTTGAGAAGC CCATCATCCT GAAGGGCCGA
     6061 TTCTGGATCA AGAAGGACAT CGTCAAGGAC TACAAGAAAA AGATTGAGGA CGCCATGGTG
     6121 GACATCCGCG ACGGACTGTA TCCTATCGGC GGCAAGACAG GCATCGGCTA CGGCTGGGTT
     6181 ACCGATCTGA CCATCCTGAA TCCTCAGAGC GGCTTTCAGA TCCCCGTGAA GAAGGATATC
     6241 AGCCCTGAGC CTGGCACCTA CAGCACATAC CCTAGCCACA GCACACCTAG CCTGAACAAA
     6301 GGCCACATCT ACTACCCTCA CTACTTTCTG GCCCCTGCCA ACACCGTGCA CCGCGAGCAA
     6361 GAAATGATCG GACACGAGCA GTTCCACAAA GAGCAGAAAG GCGAGCTGCT GGTGTCCGGC
     6421 AAGATCGTGT GCACCCTGAA AACCGTGACA CCCCTGATCA TCCCCGACAC CGAGAACGAG
     6481 GATGCCTTCG GGCTGCAGAA TACCTACAGC GGCCACAAGA ACTACCAGTT CTTTCACATC
     6541 AACGACGAGA TCATGGTGCC CGGCAGCGAG ATCAGGGGCA TGATCAGCTC TGTGTACGAG
     6601 GCCATCACCA ATAGCTGCTT CCGGGTGTAC GACGAGACAA AGTACATCAC CAGACGGCTG
     6661 AGCCCCGAGA AAAAGGACGA GAGCAACGAC AAGAACAAGT CCCAGGACGA CGCCAGCCAG
     6721 AAGATCAGAA AGGGCCTCGT GAAGAAAACC GACGAGGGCT TCAGCATCAT CGAGGTGGAA
     6781 CGGTACTCCA TGAAGACAAA AGGCGGCACC AAGCTGGTGG ACAAGGTGTA CAGACTGCCC
     6841 CTGTACGATA GCGAGGCCGT GATTGCCAGC ATCCAGTTCG AGCAGTACGG CGAGAAGAAT
     6901 GAGAAACGGA ACGCCAAGAT CAGGGCCGCC ATCAAGAGAA ACGAAGTGAT CGCCGAGGTG
     6961 GCCCGGAAGA ACCTGATTTT CCTGCGGTCC CTGACACCTG AAGAACTGAA GAAGGTGCTG
     7021 CAGGGCGAGA TCCTGGTCAA GTTCTCCCTG AAGTCTGGCA AGAACCCCAA CGACTACCTG
     7081 GCCGAGCTGC ACGAGAATGG CACAGAAAGG GGCCTGATCA AGTTCACCGG CCTGAACATG
     7141 GTCAATATCA AGAACGTGAA CGAAGAGGAC AAGGACTTCA ATGACACCTG GGACTGGGAG
     7201 AAACTGAACA TCTTCCACAA CGCCCACGAG AAGAGGAATA GCCTGAAGCA GGGCTACCCC
     7261 AGACCTGTGC TGAAGTTCAT CAAGGACCGG GTCGAGTACA CAATCCCCAA GAGATGCGAG
     7321 AGGATCTTCT GCATTCCTGT GAAGAACACC ATCGAGTACA AGGTGTCCAG CAAAGTCTGC
     7381 AAGCAGTACA AGGACGTGCT GAGCGATTAC GAGAAGAACT TTGGCCACAT CAACAAGATT
     7441 TTCACCACCA AGATCCAGAA GCGCGAGCTG ACCGACGGCG ATCTGGTGTA CTTCATCCCT
     7501 AACGAGGGCG CCGACAAGAC CGTGCAGGCT ATTATGCCAG TGCCACTGAG CAGAATCACC
     7561 GATAGCAGAA CACTGGGAGA GAGACTGCCT CACAAGAACC TGCTGCCTTG CGTGCACGAA
     7621 GTGAATGAGG GCCTGCTGTC CGGCATCCTG GACAGCCTGG ATAAGAAACT GCTGTCTATT
     7681 CACCCCGAGG GACTGTGCCC TACCTGCAGA CTGTTTGGCA CCACCTACTA CAAGGGCAGA
     7741 GTCAGATTCG GCTTCGCCAA CCTCATGAAC AAGCCCAAGT GGCTGACCGA GAGAGAGAAC
     7801 GGCTGCGGCG GATATGTGAC ACTGCCACTG CTTGAGCGGC CCAGACTGAC TTGGAGTGTG
     7861 CCCAGCGACA AGTGTGACGT GCCAGGCCGG AAGTTCTACA TCCACCACAA CGGCTGGCAA
     7921 GAGGTCCTGA GAAACAACGA CATCACCCCT AAGACCGAGA ACAACCGGAC CGTGGAACCA
     7981 CTGGCCGCCG ACAACAGATT CACCTTCGAC GTGTACTTCG AGAACCTCAG AGAGTGGGAG
     8041 CTGGGGCTGC TGTGCTATTG CCTGGAACTG GAACCTGGCA TGGGCCACAA GCTCGGAATG
     8101 GGCAAGCCTA TGGGCTTCGG CTCTGTGAAG ATCGCCATTG AGCGGCTGCA GACCTTCACA
     8161 GTGCACCAGG ATGGCATCAA CTGGAAGCCC AGCGAGAATG AGATCGGCGT GTACGTGCAG
     8221 AAAGGACGGG AAAAACTGGT GGAATGGTTC ACCCCTAGCG CTCCCCACAA GAATATGGAA
     8281 TGGAACGGCG TGAAGCACAT TAAGGACCTG AGATCCCTCC TGAGCATCCC TGGCGATAAG
     8341 CCTACCGTGA AGTACCCCAC TCTCAACAAG GATGCCGAAG GCGCCATCAG CGACTACACC
     8401 TACGAGAGAC TGAGCGATAC CAAGCTGCTG CCCCACGATA AGAGAGTGGA ATACCTGAGA
     8461 ACCCCTTGGA GCCCCTGGAA CGCCTTTGTG AAAGAGGCCG AGTACAGCCC CTCCGAGAAG
     8521 TCCGATGAGA AAGGCAGAGA GACAATCCGG ACCAAGCCTA AGAGCCTGCC AAGCGTGAAG
     8581 TCTATCGGCA AAGTGAAGTG GTTCGACGAA GGCAAAGGCT TCGGGATCCT GATCATGGAC
     8641 GATGGCAAAG AGGTGTCCAT CTCCAAGAAC AGCATCAGAG GCAACATCCT GCTCAAGAAG
     8701 GGACAGAAAG TCACCTTCCA CATCGTGCAG GGACTGATCC CTAAGGCCGA GGATATCGAG
     8761 ATTGCCAAGT GAgcggccgc actcgaggcc cgaaaggaag ctgagttggc tgctgccacc
     8821 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     8881 ctgaaaggag gaactatatc cggatatccc gcaagaggcc cggcagtacc ggcataacca
     8941 agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag
     9001 atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa
     9061 gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata
     9121 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     9181 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     9241 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     9301 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     9361 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     9421 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     9481 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     9541 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     9601 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     9661 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     9721 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     9781 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     9841 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     9901 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     9961 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
    10021 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
    10081 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
    10141 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
    10201 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    10261 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    10321 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    10381 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    10441 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
    10501 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    10561 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    10621 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    10681 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    10741 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    10801 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    10861 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    10921 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    10981 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    11041 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
    11101 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
    11161 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
    11221 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
    11281 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
    11341 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
    11401 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
    11461 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
    11521 tt
//