LOCUS       pDF0229_Golden_Gate_sit 7288 bp ds-DNA     circular     22-JUL-2020
DEFINITION  .
ACCESSION   urn.local...2a6-c6zfre4                                            
SOURCE      Cloning vector pACYC184                                            
  ORGANISM  Cloning vector pACYC184 other sequences; artificial sequences;     
            vectors.
FEATURES             Location/Qualifiers
     misc_feature    1..1
                     /label="Geneious type: Editing History Deletion"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1..1141
                     /label="Geneious type: Editing History Replacement"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1977..2115
                     /label="Golden gate insert piece"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1978..2012
                     /label="synthetic promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1982..2004
                     /label="NGS F"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2013..2047
                     /label="DR"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     source          2013..2100
                     /label="type strain of Desulfonema ishimotonii"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2069..2103
                     /label="Final DR (end 3 bases differ)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(2112..2115)
                     /label="NGS R"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2116..2150
                     /label="J23119 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2159..2170
                     /label="B0034 RBS (BBa_J34801)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2177..3976
                     /label="Twist piece 1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     source          2177..6979
                     /label="source"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2177..6979
                     /label="Translation 2177-6979"
     misc_feature    2177..6982
                     /label="E coli codon optiimized DsiCas15"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             2177..6982
                     /label="Translation 2177-6982"
     CDS             2177..6982
                     /label="Translation 2177-6982"
     misc_feature    3977..5776
                     /label="Twist piece 2"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    5777..6979
                     /label="Twist piece 3"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    6991..7119
                     /label="terminator B0015"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     source          7120..7288
                     /label="type strain of Desulfonema ishimotonii"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    7120..7288
                     /label="misc_feature"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             7120..7145
                     /label="DENIS_1082"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     gene            7120..7145
                     /label="DENIS_1082"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    7120..7145
                     /label=" CRISPR-associated RAMP family protein CDS"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             7120..7145
                     /label="Translation 7120-7145"
     repeat_region   7206..7231
                     /label="repeat_region"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     repeat_region   7210..7238
                     /label="repeat_region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     repeat_region   7253..7288
                     /label="repeat_region"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     repeat_region   7254..7288
                     /label="repeat_region"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     repeat_region   7255..7288
                     /label="repeat_region"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     repeat_region   7256..7288
                     /label="repeat_region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     repeat_region   7256..7288
                     /label="repeat_region"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    7265..7286
                     /label="Reverse primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 gaccgggtcg aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag
       61 gcgtagcaac caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc
      121 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
      181 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
      241 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
      301 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
      361 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
      421 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
      481 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
      541 gccatacgga attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
      601 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
      661 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
      721 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
      781 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
      841 tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccaaaa gttggcccag
      901 ggcttcccgg tatcaacagg gacaccagga tttatttatt ctgcgaagtg atcttccgtc
      961 acaggtattt attcggcgca aagtgcgtcg ggtgatgctg ccaacttact gatttagtgt
     1021 atgatggtgt ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc
     1081 agctactgac ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctagcggag
     1141 tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca
     1201 ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt
     1261 cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg
     1321 aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc
     1381 cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
     1441 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1501 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     1561 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     1621 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact
     1681 atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta
     1741 attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca
     1801 agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag
     1861 agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg
     1921 cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata tttctagttg
     1981 acggctagct cagtcctagg tacagtgCTA GCGGTTGgaa agccggtttt ctttgatGTC
     2041 ACGGAACcga gacctactgt tggtctcggg ttggaaagcc ggttttcttt gatgtcaCGG
     2101 TTGCCACccG GATCCTTGAC AGCTAGCTCA GTCCTAGGTA TAATGCTAGC tactagagaa
     2161 agaggagaaa tactagATGA CCACTACCAT GAAGATCAGC ATCGAGTTCT TGGAGCCCTT
     2221 CCGCATGACG AAGTGGCAGG AGTCCACGCG CCGCAATAAG AACAATAAAG AGTTTGTGCG
     2281 TGGCCAAGCG TTTGCCCGTT GGCACCGCAA CAAGAAAGAT AACACCAAGG GTCGTCCGTA
     2341 CATTACTGGT ACGCTCCTGC GCTCAGCGGT GATTCGCTCC GCGgagAACC TGCTGACACT
     2401 GAGTGACGGT AAGATCTCCG AGAAAACCTG CTGCCCGGGG AAATTCGACA CGGAAGATAA
     2461 GGACCGTTTA CTCCAGCTGC GTCAACGCTC CACGCTGCGT TGGACAGACA AGAATCCGTG
     2521 TCCCGACAAT GCGGAAACGT ATTGTCCGTT CTGTGAGCTC CTCGGCCGCT CCGGAAATGA
     2581 CGGTAAGAAA GCAGAAAAGA AGgacTGGCG CTTCCGCATC CATTTTGGTA ACCTTTCCCT
     2641 GCCGGGCAAG CCCGACTTCG ACGGTCCGAA GGCAATTGGT TCACAGCGCG TATTAAATCG
     2701 TGTGGATTTT AAGTCGGGAA AGGCACATGA CTTCTTCAAG GCTTATGAGG TGGACCATAC
     2761 CCGCTTCCCT CGCTTTGAAG GGGAGATCAC CATCGACAAT AAAGTGTCCG CCGAGGCACG
     2821 TAAGTTGCTG TGTGACAGCC TTAAATTCAC GGATCGCCTG TGTGGAGCCC TGTGCGTCAT
     2881 CCGCTTCGAC GAGTATACAC CGGCCGCGGA CAGCGGTAAG CAAACCGAGA ATGTGCAGGC
     2941 AGAGCCCAAC GCAAACCTGG CGGAGAAAAC TGCCGAGCAA ATTATCTCGA TCCTCGATGA
     3001 CAACAAGAAA ACGGAATACA CCCGCCTTTT AGCGGACGCC ATCCGCTCAT TGCGTCGCTC
     3061 CTCAAAACTT GTTGCAGGTT TGCCCAAGGA TCACGATGGT AAGGACGATC ATTACTTGtg
     3121 gGACATTGGG AAGAAGAAGA AGGATGAGAA TTCGGTTACC ATCCGTCAGA TTCTTACCAC
     3181 GAGTGCCGAC ACGAAGGAGC TGAAGAACGC TGGTAAGTGG CGCGAGTTTT GTGAGAAGCT
     3241 TGGTGAGGCG CTTTATTTGA AGAGTAAGGA TATGAGCGGC GGGTTGAAGA TTACACGCCG
     3301 TATTCTCGGG GACGCAGAGT TTCATGGCAA GCCAGATCGC TTGGAAAAGT CCCGTAGTGT
     3361 CTCGATCGGA AGCGTGTTAA AGGAAACCGT GGTGTGCGGA GAACTCGTCG CAAAGACCCC
     3421 GTTCTTCTTT GGCGCGATCG ACGAGGATGC AAAACAAACT gacTTACAGG TACTGCTTAC
     3481 TCCGGACAAT AAGTATCGCC TGCCGCGCAG CGCCGTCCGC GGCATCCTTC GCCGCGATCT
     3541 TCAAACGTAC TTTGACAGTC CCTGTAATGC GGAGTTAGGC GGTCGTCCAT GTATGTGCAA
     3601 GACCTGTCGT ATCATGCGTG GCATTACGGT TATGgacGCG CGCTCCGAGT ATAACGCGCC
     3661 CCCGGAGATT CGTCACCGTA CCCGCATTAA TCCGTTTACG GGAACAGTTG CTGAGGGCGC
     3721 CCTGTTTAAT ATGGAAGTGG CCCCGGAAGG GATCGTATTC CCATTCCAAT TACGCTATCG
     3781 CGGCTCTGAG GACGGGCTCC CAGACGCGCT CAAGACCGTG CTGAAGTGGT GGGCAGAGGG
     3841 GCAAGCTTTT ATGTCAGGGG CCGCCAGTAC CGGGAAAGGC CGTTTTCGTA TGGAGAATGC
     3901 CAAATATGAA ACCTTGGATC TGAGCGATGA GAATCAACGC AATGACTACT TGAAGAATTG
     3961 GGGCTGGCGT GACGAGAAGG GTCTTGAGGA GCTTAAGAAA CGTTTAAATT CAGGTCTGCC
     4021 GGAGCCTGGC AATTACCGTG ACCCGAAGTG GCATGAAATC AACGTGTCAA TCGAGATGGC
     4081 GAGTCCCTTC ATCAACGGCg acCCTATCCG CGCAGCTGTA GACAAGCGTG GGACGgacGT
     4141 CGTGACCTTC GTTAAGTACA AGGCGGAAGG GGAAGAAGCA AAGCCCGTGT GCGCATATAA
     4201 GGCGGAGTCG TTTCGCGGCG TCATTCGTTC GGCTGTAGCA CGCATTCACA TGGAAGATGG
     4261 AGTGCCCCTG ACTGAGCTTA CTCACTCAGA TTGCGAGTGT TTGCTCTGTC AAATCTTCGG
     4321 GTCGGAATAT GAGGCTGGTA AGATTCGTTT CGAAgatTTA GTATTTGAGA GTgacCCGGA
     4381 GCCGGTCACC TTCGATCACG TTGCAATCga cCGCTTCACC GGCGGTGCCG CTGACAAGAA
     4441 GAAGTTCGAC GACAGTCCTT TGCCAGGGTC GCCGGCGCGC CCTCTGATGC TGAAGGGTTC
     4501 CTTCTGGATT CGCCGTGACG TTCTCGAAGA TGAGGAGTAT TGCAAAGCGC TCGGGAAAGC
     4561 ATTAGCCGAT GTAAATAATG GTTTATATCC TCTGGGTGGA AAATCCGCCA TCGGATACGG
     4621 ACAAGTTAAG AGCCTGGGTA TCAAGGGCGA CGATAAGCGT ATCAGCCGTT TAATGAATCC
     4681 GGCCTTCGAC GAAACCGACG TGGCGGTTCC AGAGAAGCCT AAAACCGATG CCGAGGTCCG
     4741 TATCGAAGCT GAGAAGGTAT ATTATCCCCA CTACTTTGTG GAACCGCACA AGAAGGTAGA
     4801 GCGCGAGGAG AAGCCGTGCG GTCATCAAAA GTTTCATGAG GGACGCCTTA CCGGTAAGAT
     4861 TCGTTGTAAA CTTATTACGA AAACGCCTCT CATTGTGCCG GACACCAGCA ATGATGACTT
     4921 CTTTCGCCCT GCGGACAAAG AGGCCCGCAA GGAGAAGGAC GAGTATCACA AATCCTATGC
     4981 CTTCTTTCGC CTGCATAAGC AAATCATGAT CCCCGGTAGT GAACTCCGCG GGATGGTGTC
     5041 CTCTGTTTAC GAAACGGTTA CAAATTCATG TTTCCGTATC TTTGACGAGA CTAAGCGTCT
     5101 TAGTTGGCGC ATGGATGCCG ATCACCAAAA CGTTTTACAA GATTTCTTAC CGGGCCGTGT
     5161 TACGGCCGAC GGCAAGCACA TTCAAAAGTT TTCCGAGACT GCACGTGTAC CGTTTTATga
     5221 cAAAACGCAA AAGCACTTTG ACATTCTCGA CGAGCAGGAA ATTGCCGGTG AAAAGCCGGT
     5281 CCGCATGTGG GTGAAGCGTT TCATTAAGCG CCTGAGCCTG GTGGACCCTG CAAAGCACCC
     5341 ACAAAAGAAG CAAGACAATA AGTGGAAACG CCGCAAGGAA GGTATTGCGA CGTTCATTGA
     5401 GCAGAAGAAT GGGTCTTACT ATTTTAACGT GGTCACCAAC AATGGCTGCA CCAGCTTTCA
     5461 CTTGTGGCAC AAGCCAGACA ACTTCGACCA AGAGAAGTTA GAGGGCATCC AAAACGGGGA
     5521 GAAGTTAgat TGCTGGGTTC GTGATAGCCG TTACCAAAAG GCATTTCAAG AAATCCCGGA
     5581 GAACGATCCG GACGGATGGG AGTGTAAGGA AGGTTATCTG CATGTGGTGG GCCCTTCGAA
     5641 GGTTGAATTT AGCGACAAGA AGGGCGATGT CATTAATAAC TTCCAAGGGA CTCTCCCATC
     5701 AGTGCCCAAT GATTGGAAAA CGATTCGTAC AAACGACTTT AAGAACCGCA AACGTAAGAA
     5761 CGAGCCCGTT TTCTGTTGCG AGGATGACAA GGGGAACTAC TACACGATGG CCAAATATTG
     5821 TGAAACGTTC TTCTTCGACC TGAAAGAGAA CGAGGAGTAT GAGATCCCCG AGAAGGCACG
     5881 TATCAAGTAC AAGGAGTTAT TACGCGTGTA CAACAACAAT CCGCAAGCGG TTCCCGAGAG
     5941 CGTGTTCCAG AGTCGCGTAG CGCGCGAGAA CGTAGAAAAG CTGAAGTCTG GGGATTTGGT
     6001 GTATTTCAAG CACAACGAGA AATATGTTGA GGACATCGTA CCGGTTCGTA TTTCACGTAC
     6061 CGTAGACGAC CGCATGATCG GAAAGCGTAT GTCGGCTGAT TTGCGTCCAT GTCACGGTGA
     6121 CTGGGTAGAG GACGGGGACC TCTCTGCGTT GAATGCATAT CCAGAGAAGC GCCTTTTATT
     6181 GCGTCACCCT AAGGGCCTTT GCCCGGCATG TCGCCTTTTC GGAACGGGTA GCTACAAGGG
     6241 CCGCGTTCGT TTTGGCTTCG CGAGCCTGGA GAACgacCCG GAGTGGTTGA TTCCTGGAAA
     6301 GAATCCCGGT GATCCATTCC ACGGCGGGCC AGTGATGCTG AGCTTGCTGG AGCGCCCGCG
     6361 TCCCACCTGG AGCATTCCTG GCAGTGATAA TAAGTTCAAA GTTCCAGGCC GTAAGTTCTA
     6421 CGTTCACCAT CATGCTTGGA AAACGATTAA GGACGGTAAC CATCCGACCA CCGGCAAAGC
     6481 CATTGAACAA TCGCCAAATA ACCGCACGGT TGAAGCGCTC GCCGGTGGCA ACAGCTTCAG
     6541 CTTCGAAATT GCGTTCGAAA ATTTGAAGGA GTGGGAGTTG GGCCTGCTGA TCCATAGCCT
     6601 GCAGCTTGAA AAGGGCTTGG CCCATAAGCT CGGTATGGCT AAGAGCATGG GCTTTGGCTC
     6661 AGTAGAGATC GACGTTGAGA GTGTCCGCCT GCGTAAAGAT TGGAAGCAGT GGCGCAATGG
     6721 TAATTCAGAG ATCCCAAATT GGTTGGGCAA GGGGTTTGCA AAGCTGAAGG AGTGGTTCCG
     6781 CGACGAGCTG GACTTCATCG AGAATTTGAA GAAATTACTG TGGTTCCCCG AAGGTGATCA
     6841 AGCTCCGCGC GTATGTTACC CCATGCTTCG CAAGAAGGAT GACCCTAATG GAAACTCAGG
     6901 TTATGAGGAG TTGAAAGACG GCGAATTTAA GAAGGAAGAT CGTCAGAAGA AGCTTACAAC
     6961 ACCATGGACG CCGTGGGCTT GAtactagag ccaggcatca aataaaacga aaggctcagt
     7021 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     7081 acactggctc accttcgggt gggcctttct gcgtttatac cacgccttgg accccttggg
     7141 cctgatcttc tgtgccgcct gatactctcc cttcgtaaga tgattcagca tgaaaagttc
     7201 attatttcag cccggaagtt cattttcggg ctgaaacctg ttggtttcct ccgataaccc
     7261 gcttcagcgg gtttcaataa ctgagccg
//