LOCUS       Exported               11934 bp ds-DNA     circular SYN 14-JUN-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11934)
  AUTHORS   diannelumaquin
  TITLE     Direct Submission
  JOURNAL   Exported Jun 14, 2021 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11934
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    7..560
                     /label=Tol2 R
                     /note="color: #808000"
     primer_bind     complement(557..573)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     promoter        complement(610..628)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(649..665)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    673..689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(697..727)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 697 .. 703 / #ffffff / -10
                        2: 704 .. 721 / #ffffff
                        3: 722 .. 727 / #ffffff / -35"
     protein_bind    742..763
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(1051..1639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(1810..2670)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 1810 .. 2601 / #ccffcc
                        2: 2602 .. 2670 / #ccffcc / signal sequence
                     Cleavage site after base 2601"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2671..2775)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    3023..3843
                     /label=Tol2 L
                     /note="color: #808000"
     primer_bind     3855..3871
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     protein_bind    3891..3911
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
                     /note="color: #31849b; direction: RIGHT"
     promoter        3912..7445
                     /label=Zubi_promoter
                     /note="color: #cc99ff"
     protein_bind    7446..7470
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    7454..7470
                     /label=attB1 forward
                     /note="color: #a6acb3"
     CDS             7491..8822
                     /codon_start=1
                     /label=Plin2 cDNA
                     /note="color: #993366"
                     /translation="MSFLLYLKLHNRFSANMGSMEDVNNQNVVARIVHLPLVVSTYDMM
                     SNVYVNTKNNHPYLKSVCEVAENGVKSITSAAFHRSLPIIGKLEPQISMANDLACKGLD
                     KIEKTLPILHKPSEQIMANAKDAVTGAKEAVSGTVSGAKDSVSFTLNDVMDRTRGAVQG
                     SVEKTKTVVSGGVQTVMDSRMLKLVSSRVDNALSTSETLLEHYLPENEEEKEGEERNTE
                     GLENDSDLANYYVRLGSLSIKVRDRTYQRAVEKVRHAVQRGQESISQLNHTMDIIEFTR
                     KNIDGSNQKLQEKLGSLMGWKSVSQSQETESETDSEAEQIESRTLTIAHSLSQQLQSTC
                     VVLVSSLRGLPQHVQLQVGSVSRSALEIYSSFSKAAVLGDLSSTVLSSSRSQLSRITES
                     MDNVMDYLLNNTPLNWLVGPFYPRVERAVENEAKCSKQRTLQVTE"
     CDS             8823..10253
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    complement(10274..10298)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     polyA_signal    10300..10434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     protein_bind    complement(10497..10517)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
                     /note="color: #31849b; direction: LEFT"
     primer_bind     complement(10525..10541)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     polyA_signal    10557..10691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        10813..11143
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      10994..11129
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        11191..11238
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             11257..11655
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /note="color: #ccffcc"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    11813..11934
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
ORIGIN
        1 agatctgcga agatacggcc acgggtgctc ttgatcctgt ggctgatttt ggactgtgct
       61 gctcgcagct gctgatgaat cacatacttc ctccattttc ttccactgat tgactgttat
      121 aatttcccta atttccaggt caaggtgctg tgcattgtgg taatagatgt gacatgacgt
      181 cacttccaaa ggaccaatga acatgtctga ccaatttcat ataatgtgaa aacgattttc
      241 ataggcagaa taaataacat ttaaattaaa ctgggcatca gcgcaattca attggtttgg
      301 taatagcaag ggaaaataga atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa
      361 ataaaattgt aaggagtaaa aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt
      421 aaaagtattg atttttaatt gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa
      481 gtacagtaat caagtaaaat tactcaagta ctttacacct ctggttcttg accccctacc
      541 ttcagcaagc ccagcagatc cactagttct agagcggccg ccaccgcggt ggagctccag
      601 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
      661 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
      721 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
      781 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
      841 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
      901 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
      961 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1021 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1081 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1141 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1201 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1261 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1321 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1381 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1441 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     1501 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1561 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1621 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1681 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1741 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1801 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     1861 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     1921 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     1981 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2041 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2101 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2161 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     2221 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     2281 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     2341 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     2401 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     2461 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     2521 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     2581 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     2641 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     2701 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     2761 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     2821 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     2881 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     2941 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     3001 gtctatcagg gcgatggccc acgctggcta agaactcatc agcctccccg gtccatctac
     3061 ccacgtacca atgcaccaat tggccacaat gacggctact acatggtgcc attccttcct
     3121 ctttatagga atggagacta cctcctgtcc aacaaggctc ttggatacga gtacgcctac
     3181 ctgttggacc caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga
     3241 atatctgttc agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag
     3301 ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt
     3361 agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa
     3421 gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag
     3481 aaagaaaact agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca
     3541 tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac
     3601 aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa
     3661 gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg
     3721 cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag
     3781 agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca
     3841 gatgggccct cgagcaggaa acagctatga ccatgattac gccaagctat caactttgta
     3901 tagaaaagtt ggctccgaat tcgcccttaa aactcgagac cagcaaagtt ctagaatttg
     3961 tcgaaacatt tatgttatat atttcctgaa aaaaattctg agtaagttct taagtgttat
     4021 tgccagcaac ataaacaaca gacggcaaaa tgaataaatg ataacaaagc agtaggctta
     4081 aataaaccta atttttatag gctgttctct acaaccctca aacagtgatt agttttgtac
     4141 ttataaactt gccctttcat tcatatttca agaaaattgg ttcagaagat ctggatattc
     4201 tagcagttgt tcaagctcat ggagggatca gtgacctgat tccacaatga ctaggcctaa
     4261 tccagaaatt agatgactgt caacataaaa aggcacagca ctcactagct gccctatata
     4321 ttttattata ttttacatat attattttat ttatttagct ctgagtgctg tactttctgg
     4381 ttaaagaaaa ctgcttacaa cagctaacct gtactacctc aggctcaggg aatttggaac
     4441 aggtttgtct ggtttgtttc tttaaccatg catgcttgtt ttcaactatg gcaacacagt
     4501 cacatgggac attacagaaa tgatttgtcg atgacatgcg acttttcttt aataaagcgc
     4561 aaagatccca aaaagcaaac ttttaacaaa aatcatataa ttatattttc aatccagctt
     4621 tgtagcaact ttgtgctgct gttcactcag caacagatag tcagtataag gtcagtgtgt
     4681 ctcaaagcag tgccatctgt ttcacacatt gcgttctata tataagtgtg ctggttgaca
     4741 cgacactgta taaggcctag gctaaaacac aaacaatgta gaatgacact gtgttttttt
     4801 tgtaaacaaa tgttgttttt ggttaaacat ctttgtgaaa acatcctcct gtcatgtatt
     4861 tgctatattc aaatgttaaa cccgtgcaga atagaacata tacaaaaaaa aacaacacaa
     4921 cacattttta aacattatta aatatcaagt attgctggca gttctgtttc tgttttacag
     4981 taccctttgc cacagttctc cgcttttcct ggtccagatt ccacaagtct gattcaccaa
     5041 tagcaaagcg aataaacaac caaagcagcc aatcactgct tgtagactgt cctgcgagac
     5101 cggcccattc cagcacattc tggaaacttc ctttatatga taattataaa tacatttaaa
     5161 ttattgatac aaaacatgta attcctagaa cataaccata gcaatcatta gttttcaggg
     5221 taattatgta tttttaggat ttgactgcgg aaagatctgg tcatgtgacg tctcatgaac
     5281 gtcacggccc tgggtttcta taaatacagt aggactctcg accatcggca gatttttcga
     5341 agaagaagat cagtttcagg agccgtactg ttccgtttca acgcaaatat taacggtaag
     5401 agcgaatttc ctagtttgtt ttcatgccat tctttaaaac catagcgtat tactttaatt
     5461 atagtaaact ttcgctttct ttattacaag agacgttttg tgttgattct ccgcggacat
     5521 tttcggtcag acaatcagaa aatgaccgcg gaggaccagt aacttgcatt acacgtaagt
     5581 taaatcttcg tgtattaaaa tggttaggtt gttaacgtca aataggttac cgtgtttgcg
     5641 tgtgatcagg ttggttttgt tagatttttg tcagtatttt taatttattt gttttagttt
     5701 atttattttt tttgctgaat catagtttgt gaacaaagaa cccggatgtt acatacagta
     5761 cagccgccat gttacagaga gttataactt aatcatttta aaaataattt tgccttactt
     5821 ttagtttgtc atgttgagaa atgaggaaat gttaaaatga ggaaatatcc aattaattta
     5881 atatatcaaa ataatccatg attacaatgc actgaactgg agaaaattaa gatgttttct
     5941 agtgtcatga aacaaatgta agagatgtac attgtagatg ttttatgtca agaattggct
     6001 agttgatgca gcatactggc gatactcagt tgtaataaca gtaacgttac atgttaatag
     6061 actactgagt atgctgttct gtctatgtat gctctgtaag ctacgagaag gactttttta
     6121 aacagtaaag ggtgcaatat ttttacaaat tgaattaaat aaaggctgtc tattaagtaa
     6181 tatgcttgat atttttctta cttgatcgga aataagaaaa aatataaacg ttgttgctct
     6241 aaaaatccta gttcagttta gccaaccaca aatacctttt tgttcctcca acagtttttt
     6301 tttcttctct ataatatttg gcagtctata gtactccaaa tgtttcccca cagtctaact
     6361 aattggtaca gccaaaatca tgacacttat tgcaataata attttggttc attggcattg
     6421 ttgatagcct gtgccactaa tatggtcgat tgatcatgct tcaggaagaa aactatattg
     6481 tttgatgtaa gattattaaa tcttcacctg cttccattac aaactattcc catcttattg
     6541 aattctggta tgtcttaaag gattagttca cttcccaaat caaaatttac ttagcctttt
     6601 ttcatccatg atcccttttt ttcatcatta atgaagaaat tgtttttgaa aaagtttcaa
     6661 gatttttttc tctatattgt ggagcttgtt agtttaaaat tccaaaatgc aatatgtggc
     6721 ttcaaatggt tctaaatgat cccagtcaag gaataacagt cttatctaat gaaaccatta
     6781 gacctttaaa aataaaaaaa ataaaagtat ttatttttaa atgactgagt gattaagttg
     6841 aatttcagcg tttccttact gtgtagaagt ccttccttac tggccccacc ctttggttct
     6901 ctgccaatct gctacctaat gtaatgttgt ggaacattat tattctttat ttcttaattt
     6961 tttatttttt attttaaaac aatgtaaact gcacagatgt gcagtttgtt taaaatggcc
     7021 aatgctttgg aaatgcatga cataattaga tttcatgatg cacaaagcca aatctcagag
     7081 cttgtgcaaa atgagctatc atttcactag gtaagaccct aaattttcat ataggatcat
     7141 ttggacaatt ttgctgcagg taaaatgcat tctatagtcc actgtcagcc attgttttgg
     7201 atagtattta tttttctcta caagtatagt caatagtttt ctattatttt aaaggtttgt
     7261 aacatttaag ggtgaccaaa tgcaaagtaa aatttcattt tcgggtgaac tatctcgttt
     7321 aacatgggag aagtgcaaaa catacattat tggctagaac attgtagtat tttttaaatg
     7381 gaaatgtgtg attgctaatc ttactttgaa tttgtttaca gggatccaaa aagggcgaat
     7441 tcgacccaag tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atgagctttc
     7501 ttctgtactt gaaactgcac aacagatttt ctgctaacat gggttctatg gaggatgtaa
     7561 ataaccagaa tgtggttgcc cgaattgttc accttcccct ggttgtttcc acctatgaca
     7621 tgatgtcgaa cgtgtacgtc aacaccaaga acaaccaccc atacctgaag tctgtgtgcg
     7681 aggttgctga gaatggtgtc aaatccataa cctcagctgc ctttcacagg tctttaccaa
     7741 tcattggcaa actggagcct cagatctcta tggcaaatga cctggcgtgc aaaggactgg
     7801 ataagataga aaagactctg cccatcctac acaagccatc tgaacagatt atggccaatg
     7861 ctaaagatgc agtgacagga gccaaggagg ctgtcagcgg gactgtgagc ggtgccaagg
     7921 attctgtctc cttcacccta aatgatgtga tggaccgaac acgcggggca gtgcagggca
     7981 gcgtggagaa gaccaagact gtggtcagcg gtggagtcca gacagtgatg gacagcagga
     8041 tgctcaaact ggtgtccagc agagtggaca atgcactgag cacatctgag acgctgctgg
     8101 agcactactt gcctgaaaat gaggaggaaa aagaaggtga agagaggaat actgaaggat
     8161 tggaaaatga cagcgatttg gccaattatt atgtccgact gggctctctg tccattaaag
     8221 tcagagacag gacttatcag agagccgtgg agaaagttcg tcatgccgtg cagcgcggac
     8281 aggagtccat ttcacagctt aaccacacaa tggatataat cgaatttacc aggaagaata
     8341 ttgatgggtc taaccagaag ctgcaggaga aactgggttc actaatgggc tggaagagcg
     8401 tttctcaaag ccaagaaaca gagagcgaaa cagacagtga agctgagcaa attgagtcgc
     8461 gcacactgac catcgcccac agcctcagcc agcagcttca gagcacatgt gtggtgctgg
     8521 tgtccagtct caggggtctc cctcagcacg tccagctcca ggtgggctcc gtcagccgct
     8581 ccgcattgga gatctacagc agcttcagta aagcggccgt gctgggggat ctgtccagca
     8641 ccgtcctgtc ctccagccgc tcacagctca gcagaatcac agagtccatg gacaacgtga
     8701 tggactacct gctcaacaac actcctctca actggctggt cggaccgttt tacccacgtg
     8761 tggagcgcgc cgttgaaaac gaggccaaat gtagcaaaca gaggaccctt caagtcactg
     8821 aaatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg
     8881 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     8941 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     9001 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca
     9061 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     9121 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga
     9181 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga
     9241 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
     9301 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga
     9361 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc
     9421 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg
     9481 gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca
     9541 ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc
     9601 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     9661 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     9721 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     9781 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     9841 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     9901 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     9961 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
    10021 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
    10081 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
    10141 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
    10201 ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag tagaagagcc
    10261 cgggcgatcg cgaacccagc tttcttgtac aaagtggggg atccagacat gataagatac
    10321 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
    10381 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
    10441 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttccaac
    10501 tttattatac atagttgata attcactggc cgtcgtttta cggtaccatc gatgatgatc
    10561 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
    10621 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
    10681 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
    10741 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc
    10801 tagatcagat ctgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag
    10861 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
    10921 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
    10981 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    11041 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
    11101 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
    11161 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
    11221 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa
    11281 gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac
    11341 tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta
    11401 tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg
    11461 gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc
    11521 ccctgcggac ggtgtcgaca ggtgcttctc gatctgcatc ctgggatcaa agcgatagtg
    11581 aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat
    11641 gtgtgggagg gctaagcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
    11701 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
    11761 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
    11821 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    11881 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat ctta
//
