LOCUS       pAAV-hSyn-Axon-jRex-GEC 6016 bp ds-DNA     circular     17-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      699..1287
                     /label="ori"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     1188..1207
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     repeat_region   1349..1478
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        1689..2136
                     /label="hSyn promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          2114..2133
                     /label="136_pAAV_hSyn_DIO_hM4D(Gi)_mCherry_F"
                     /note="sequence: tcgtgtcgtgcctgagagcg"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          2114..2133
                     /label="131_pAAV_hSyn_eGFPSeq1"
                     /note="sequence: TCGTGTCGTGCCTGAGAGCG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2159..2218
                     /label="Axon-targeting"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2159..2218
                     /label="Translation 2159-2218"
     misc_feature    2219..3472
                     /label="jRex-geco"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             2219..3496
                     /label="Translation 2219-3496"
     misc_feature    3497..4085
                     /label="WPRE"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(3550..3570)
                     /label="WPRE-R"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(3968..3979)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     polyA_signal    4105..4581
                     /label="hGH poly(A) signal"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(4304..4323)
                     /label="hGH-PA-R"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     repeat_region   4627..4756
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      4842..5297
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(4929..4948)
                     /label="F1ori-R"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     5139..5160
                     /label="F1ori-F"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(5314..5333)
                     /label="pRS-marker"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     5433..5455
                     /label="pGEX 3'"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(5493..5511)
                     /label="pBRforEco"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        5579..5683
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             join(5684..6016,1..528)
                     /label="AmpR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(5902..5921)
                     /label="Amp-R"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
       61 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
      121 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
      181 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
      241 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
      301 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
      361 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
      421 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
      481 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
      541 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
      601 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
      661 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
      721 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
      781 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
      841 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
      901 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
      961 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1021 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1081 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1141 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1201 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1261 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1321 gccttttgct ggccttttgc tcacatgtcc tgcaggcagc tgcgcgctcg ctcgctcact
     1381 gaggccgccc gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg
     1441 cagagaggga gtggccaact ccatcactag gggttcctgc ggccgcacgc gttgcaaaga
     1501 tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa
     1561 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     1621 gagaagttgg ggggaggggt cggcagcaaa tggttaatta atctagactg cagagggccc
     1681 tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg tgcctacctg
     1741 acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat tgcgcatccc
     1801 ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact gccagcttca
     1861 gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg cctcagcact
     1921 gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg ccaccttggt
     1981 cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga gatagggggg
     2041 cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg
     2101 ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga gaggatccac ccgccaccat
     2161 gctgtgctgt atgagaagaa ccaaacaggt tgaaaagaat gatgaggacc aaaagattat
     2221 ggtcgactct tcacgtcgta agtggaataa ggcaggtcac gcagtcagag ctataggtcg
     2281 gctgagctca cgttgggttt ccgagtggat gtaccccgag gacggcgccc tgaagagcgt
     2341 gatcaaggag gggttgaggc tgaaggacgg cggccactac gccgccgagg tcaggaccac
     2401 ctacaaggcc aaaaagcccg tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga
     2461 catcgtgtcc cacaacgagg actacaccat cgtggaacag tgcgaacgcg ccgagggccg
     2521 ccaccccacc ggcggcatgg tcgggctgta caagggaggt acaggcggga gtctggtgag
     2581 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     2641 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     2701 cgaggccttt cagaccgcta agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     2761 ggacatcctg tcccttcagt tcatgtacgg ctccaaggcc tacattaagc acccagccga
     2821 catccccgac tacttcaagc tgtccttccc cgagggcttc aggtgggagc gcgtgatgat
     2881 cttcgaggac ggcggcatta ttcacgttaa ccaggactcc tccctgcagg acggcgtatt
     2941 catctacaag gtgaagctgc gcggcaccaa cttccccccc gacggccccg taatgcagaa
     3001 gaagaccatg ggctgggagc ctacgcgtga cgatctgact gaagagcaga tcgcagagtt
     3061 taaagaggct ttctccctat ttgacaagga cggggatggg acgataacaa ccaaggagct
     3121 ggggacggtg ttccggtctc tggggcagaa ccccacagaa gcagagctgc aggacatgat
     3181 caatgaagta gatgccgacg gtgacggcac attcgacttc cctgagttcc tgacgatgat
     3241 ggcaaggaaa atgaatgact cagacagtga agaggaaatt agagaagcgt tccgcgtgtt
     3301 tgataaggac ggcaatggct acatcggcgc agcagagctt cgccacgtga tgacagacct
     3361 tggagagaag ttaacagatg aggaggttga tgaaatgatc agggtagcag acatcgatgg
     3421 ggatggtcag gtaaactacg aagagtttgt acaaatgatg acagcgaagt aggaattccg
     3481 tacaaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3541 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3601 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3661 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     3721 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     3781 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     3841 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     3901 ctttccttgg ctgctcgcct atgttgccac ctggattctg cgcgggacgt ccttctgcta
     3961 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4021 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4081 cccgcatcga taccggctcg agacgggtgg catccctgtg acccctcccc agtgcctctc
     4141 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc
     4201 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg
     4261 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc
     4321 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt
     4381 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa
     4441 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc
     4501 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc
     4561 actgctccct tccctgtcct tctcgagctg attttgtagg taaccacgtg cggaccgagc
     4621 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     4681 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     4741 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     4801 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     4861 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
     4921 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4981 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5041 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5101 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5161 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
     5221 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     5281 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     5341 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     5401 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     5461 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     5521 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     5581 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5641 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5701 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5761 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5821 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5881 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5941 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     6001 cacagaaaag catctt
//