LOCUS       Exported                9682 bp DNA     circular SYN 22-JUL-2021
DEFINITION  sfGFP DO for Part 1 (Part 1a/1b).
ACCESSION   urn.local...vb-dn74pmy
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 17, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9682
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          5411..6444
                     /label=Part 1 DO
                     /note="/created_by=moham"
                     /note="/modified_by=moham"
     primer_bind     4..20
                     /label=KJ_T0046_pcDNA5_NotI_Fwd
     primer_bind     5..20
                     /label=KJ_T0399_FLAG_Cterm_Fwd
     primer_bind     complement(37..52)
                     /label=KJ_T0020_EGFP_Rev
     terminator      42..277
                     /label=BGH-PA
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=98.31%; Adjusted-Interval-0:0/0;
                     BGH-PA (<a href=""urn:local:.:9zo-bzmwvsn"">JPF0509</a>)"
                     /note="/Alternative_Match=98.31%; Adjusted-Interval-0:0/0;
                     BGH-PA (<a href=""urn:local:.:9ww-bzmwvr7"">ARB341</a>)"
                     /note="/Alternative_Match=98.31%; Adjusted-Interval-0:0/0;
                     BGH-PA (<a href=""urn:local:.:9yc-bzmwvrt"">ARB367</a>)"
                     /note="/Alternative_Match=98.31%; Adjusted-Interval-0:0/0;
                     BGH-PA (<a href=""urn:local:.:9yo-bzmwvs4"">ARB387</a>)"
                     /note="/Alternative_Match=98.31%; Adjusted-Interval-0:0/0;
                     BGH-PA (<a href=""urn:local:.:9x4-bzmwvr9"">ARB343</a>)"
                     /note="/Alternative_Match=98.31%; Adjusted-Interval-0:0/0;
                     BGH-PA (<a href=""urn:local:.:a1s-bzmwvtt"">JPF0536b</a>)"
                     /note="/Alternative_Match=98.31%; Adjusted-Interval-0:0/0;
                     BGH-PA (<a href=""urn:local:.:a10-bzmwvtc"">JPF0521</a>)"
                     /note="/Alternative_Match=98.31%; Adjusted-Interval-0:0/0;
                     BGH-PA (<a href=""urn:local:.:a0g-bzmwvt2"">JPF0516</a>)"
                     /note="/Alternative_Match=98.31%; Adjusted-Interval-0:0/0;
                     BGH-PA (<a href=""urn:local:.:9y8-bzmwvrr"">ARB366</a>)"
                     /note="/Primary_Match=98.31%; Adjusted-Interval-0:0/0; 
                     BGH-PA
                     (<a href=""urn:local:.:a00-bzmwvst"">JPF0512</a>)"
                     /note="/Transferred_From=JPF0512"
                     /note="/Transferred_Similarity=98.31%"
     polyA_signal    53..277
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:y-c2vthvs"">pDisplay-mSA-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:7t-c2wj41q"">Lenti__IVT_gBlock_msr-msd_ 
                     (Wt)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:7n-c8vy8oc"">pDisplay-mSA-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:87-c2wj420"">Lenti_IVT_gBlock_msr-msd_( 
                     TurboRFP_repair)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:1y-c61zrev"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:1x-c61zreu"">pDisplay-SS47 
                     (86)-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:8f-c2wj425"">Lenti_sgRNA_sfGFP-DO_no_Sp 
                     yCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:1x-c7wp0k7"">pDisplay-SS47 
                     (86)-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:19-c5kw4c2"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:6a-c8vxg0z"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)"
                     /note="/Transferred_From=pDisplay-SS47 (87)-EGFP-TM"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     complement(283..299)
                     /label=KJ_T0019_PolyA_Rev
     misc_feature    complement(402..413)
                     /label=polyArg
                     /note="polyarginine tag used for protein purification (may 
                     sometime have 6 R residues instead of 5)"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on protein similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     polyArg (<a href=""urn:local:.:37-c0ul19s"">SARS-CoV-2_S-2P
                     translation 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     polyArg (<a href=""urn:local:.:e-c79sdnm"">pDisplay-SS47 
                     (87)-EGFP-TM translation</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     polyArg (<a href=""urn:local:.:u-c79srj8"">S-2P_87_TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     polyArg (<a href=""urn:local:.:2g-c0ubf2q"">SARS-CoV-2_S-2P
                     translation 1</a>)"
                     /note="/Color=#FFFFB9"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     polyArg
                     (<a href=""urn:local:.:n-c79snqp"">S-2P_86_TM</a>)"
                     /note="/Transferred_From=S-2P_86_TM"
                     /note="/Transferred_Translation_Similarity=100.00%"
                     /note="/X-Alternative_Match=80.00%; 
                     Adjusted-Interval-0:3/3; <a 
                     href=""""urn:local:.:z1-bzc80bg"""">polyArg</a>"
                     /note="/X-Primary_Match=80.00%; Adjusted-Interval-0:0/0; <a
                     href=""""urn:local:.:z1-bzc80bg"""">polyArg</a>"
                     /note="/X-Transferred_From=polyArg"
                     /note="/X-Transferred_Translation_Similarity=80.00%"
                     /note="/kind=tag"
     primer_bind     538..557
                     /label=KJ_T0022_FRT_Fwd
     protein_bind    561..608
                     /label=FRT
                     /bound_moiety="FLP recombinase from the <i>Saccharomyces
                     cerevisiae</i> 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (<a 
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/21891781"" 
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/21891781"">Tura 
                     n and Bode, 2011</a>)."
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a 
                     href=""urn:local:.:v-c0u7dc2"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a 
                     href=""urn:local:.:973-bzmvybu"">pcDNA-FRT-TO-intron</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a 
                     href=""urn:local:.:3i-c0uqp26"">pcDNA5-intron-Spike-3XFLAG 
                     -TM-2A-EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a 
                     href=""urn:local:.:3f-c45uttj"">pcDNA5_TET_intron_HA_NLS_E 
                     co2_EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:2r-c0uhwai"">RFP-GFP 
                     reporter_pcDNA5-FRT-TO-intron_v1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a 
                     href=""urn:local:.:9ft-bzmwl6g"">pcDNA5-intron_HA_NLS_Eco1 
                     _FLAG</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:97c-bzmvyby"">pcDNA5 Reporter (RFP 
                     GFP) with hPGK promoter (replaced CMV)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a 
                     href=""urn:local:.:9fn-bzmwl6e"">pcDNA5-intron_HA_NLS_Eco1 
                     _EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a 
                     href=""urn:local:.:9fk-bzmwl6d"">pcDNA5-intron_HA_NLS_Eco1 
                     </a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; FRT
                     (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Transferred_From=pcDNA5_TET_intron_HA_NLS_Eco1_EGFP
                     "
                     /note="/Transferred_Similarity=100.00%"
     rep_origin      complement(575..608)
                     /direction=LEFT
                     /label=FRT site
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=89.47%; Adjusted-Interval-0:0/0; FRT 
                     site
                     (<a href=""urn:local:.:910-bzmvb6e"">pYTK082</a>)"
                     /note="/Transferred_From=pYTK082"
                     /note="/Transferred_Similarity=89.47%"
     protein_bind    complement(575..608)
                     /standard_name="FRT (minimal)"
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (minimal) (<a 
                     href=""urn:local:.:11-c4a760j"">Mu-793_R</a>)"
                     /note="/Primary_Match=82.35%; Adjusted-Interval-0:0/0; FRT
                     (minimal) (<a 
                     href=""urn:local:.:17-c4a760m"">Mu-795_R</a>)"
                     /note="/Transferred_From=Mu-795_R"
                     /note="/Transferred_Similarity=82.35%"
     misc_feature    575..608
                     /label=FRT
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:a3k-bzmwvuk"">MTK0_037</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:9l8-bzmwsfr"">MTK0_051</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:9l8-bzmwsfr"">MTK0_051</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:a44-bzmwvus"">MTK0_053</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:a44-bzmwvus"">MTK0_053</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:a2w-bzmwvub"">MTK0_028</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:a2w-bzmwvub"">MTK0_028</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:a2s-bzmwvu9"">MTK0_012</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:a2s-bzmwvu9"">MTK0_012</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; FRT
                     (<a href=""urn:local:.:a3k-bzmwvuk"">MTK0_037</a>)"
                     /note="/Transferred_From=MTK0_037"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    complement(575..608)
                     /label=FRT
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (<a href=""urn:local:.:a3k-bzmwvuk"">MTK0_037</a>)"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (<a href=""urn:local:.:9l8-bzmwsfr"">MTK0_051</a>)"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (<a href=""urn:local:.:9l8-bzmwsfr"">MTK0_051</a>)"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (<a href=""urn:local:.:a44-bzmwvus"">MTK0_053</a>)"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (<a href=""urn:local:.:a44-bzmwvus"">MTK0_053</a>)"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (<a href=""urn:local:.:a2w-bzmwvub"">MTK0_028</a>)"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (<a href=""urn:local:.:a2w-bzmwvub"">MTK0_028</a>)"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (<a href=""urn:local:.:a2s-bzmwvu9"">MTK0_012</a>)"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (<a href=""urn:local:.:a2s-bzmwvu9"">MTK0_012</a>)"
                     /note="/Primary_Match=82.35%; Adjusted-Interval-0:0/0; FRT 
                     (<a href=""urn:local:.:a3k-bzmwvuk"">MTK0_037</a>)"
                     /note="/Transferred_From=MTK0_037"
                     /note="/Transferred_Similarity=82.35%"
     CDS             610..1638
                     /codon_start=1
                     /gene="<i>aph(4)-Ia</i>"
                     /product="aminoglycoside phosphotransferase from <i>E.
                     coli</i>"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Adjusted_Transferred_Similarity=99.42%"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:6/0
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:v-c0u7dc2"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:6/0
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:973-bzmvybu"">pcDNA-FRT-TO-intron</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:6/0
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:3i-c0uqp26"">pcDNA5-intron-Spike-3XFLAG 
                     -TM-2A-EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:6/0
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:3f-c45uttj"">pcDNA5_TET_intron_HA_NLS_E 
                     co2_EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:6/0
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:2r-c0uhwai"">RFP-GFP 
                     reporter_pcDNA5-FRT-TO-intron_v1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:6/0
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:9ft-bzmwl6g"">pcDNA5-intron_HA_NLS_Eco1 
                     _FLAG</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:6/0
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:97c-bzmvyby"">pcDNA5 Reporter (RFP GFP) 
                     with hPGK promoter (replaced CMV)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:6/0
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:9fn-bzmwl6e"">pcDNA5-intron_HA_NLS_Eco1 
                     _EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:6/0
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:9fk-bzmwl6d"">pcDNA5-intron_HA_NLS_Eco1 
                     </a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:6/0 
                     (adjusted
                     to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Transferred_From=pcDNA5_TET_intron_HA_NLS_Eco1_EGFP
                     "
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=KKPELTATSVEKFLIEKFDSVSDLMQL
                     SEGEE 
                     SRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCI 
                     SRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRD 
                     FICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTD 
                     NGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAY 
                     MLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVE 
                     VLADSGNRRPSTRPRAKE*"
                     /translation="LAKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     misc_feature    675..680
                     /label=Esp3I, BsmBI
                     /note="Geneious type: restriction site"
                     /note="/Methylation=Mixed Methylation"
                     /note="/Recognition_pattern=CGTCTC(1/5)"
                     /note="/annotation_group=CGTCTC"
     primer_bind     complement(1648..1666)
                     /label=KJ_T0017_HygR_Rev
     primer_bind     1682..1698
                     /label=KJ_T0023_SV40_Fwd
     polyA_signal    complement(1768..1902)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=98.52%; Adjusted-Interval-0:0/0; 
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:3o-c0uw9uv"">pCEP4-BaLgp140-TM</a>)"
                     /note="/Primary_Match=98.52%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:e-c0uzs7w"">pDisplay-AP-CFP-TM</a>)"
                     /note="/Transferred_From=pDisplay-AP-CFP-TM"
                     /note="/Transferred_Similarity=98.52%"
     polyA_signal    1768..1889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9bv-bzmwcmo"">Lenti_FnCas9_sfGFP 
                     DO</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:95r-bzmvybf"">mNeptune2 CRISPEY msr 
                     msd</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:72-c0q55wp"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9 - KJ_T0505_IVT_gBlock_msr-msd_(Wt)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:95x-bzmvybg"">mNeptune2 
                     CRISPEY_81mer_sgRNA1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9c9-bzmwcmt"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9ea-bzmwl5z"">3XFLAG-NLS-RT</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:1l-c0q1vfs"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9em-bzmwl63"">HA-NLS-RT-EGFP</a>)"
                     /note="/Transferred_From=HA-NLS-RT-EGFP"
                     /note="/Transferred_Similarity=100.00%"
     polyA_signal    1768..1889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9e4-bzmwint"">Exported</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:7t-c2wj41q"">Lenti__IVT_gBlock_msr-msd_ 
                     (Wt)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:87-c2wj420"">Lenti_IVT_gBlock_msr-msd_( 
                     TurboRFP_repair)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:8f-c2wj425"">Lenti_sgRNA_sfGFP-DO_no_Sp 
                     yCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9ar-bzmvydt"">Exported</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:7z-c2wj41v"">Lenti_IVT_gBlock_msr-msd_( 
                     TurboRFP_repair)_HH_and_HDV</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9ia-bzmwl77"">Exported</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9az-bzmvydy"">Exported</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9an-bzmvydr"">Exported</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9av-bzmvydw"">Exported</a>)"
                     /note="/Transferred_From=Exported"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    1771..1890
                     /label=SV40_PA_terminator
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40_PA_terminator (<a 
                     href=""urn:local:.:9ks-bzmwsfl"">MTK0_044</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40_PA_terminator (<a 
                     href=""urn:local:.:9is-bzmwset"">MTK0_006</a>)"
                     /note="/Transferred_From=MTK0_006"
                     /note="/Transferred_Similarity=100.00%"
     terminator      complement(1773..1902)
                     /label=SV40-PA
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=98.46%; Adjusted-Interval-0:0/0;
                     SV40-PA (<a href=""urn:local:.:a34-bzmwvue"">MTK0_031</a>)"
                     /note="/Alternative_Match=98.46%; Adjusted-Interval-0:0/0;
                     SV40-PA (<a href=""urn:local:.:9yc-bzmwvrt"">ARB367</a>)"
                     /note="/Alternative_Match=98.46%; Adjusted-Interval-0:0/0;
                     SV40-PA (<a href=""urn:local:.:9yo-bzmwvs4"">ARB387</a>)"
                     /note="/Alternative_Match=98.46%; Adjusted-Interval-0:0/0;
                     SV40-PA (<a href=""urn:local:.:9yo-bzmwvs4"">ARB387</a>)"
                     /note="/Alternative_Match=98.46%; Adjusted-Interval-0:0/0;
                     SV40-PA (<a href=""urn:local:.:a1s-bzmwvtt"">JPF0536b</a>)"
                     /note="/Alternative_Match=98.46%; Adjusted-Interval-0:0/0;
                     SV40-PA (<a href=""urn:local:.:a44-bzmwvus"">MTK0_053</a>)"
                     /note="/Alternative_Match=98.46%; Adjusted-Interval-0:0/0;
                     SV40-PA (<a href=""urn:local:.:9y8-bzmwvrr"">ARB366</a>)"
                     /note="/Alternative_Match=98.46%; Adjusted-Interval-0:0/0;
                     SV40-PA (<a href=""urn:local:.:a1w-bzmwvtw"">JPF0536c</a>)"
                     /note="/Alternative_Match=98.46%; Adjusted-Interval-0:0/0;
                     SV40-PA (<a href=""urn:local:.:9y4-bzmwvro"">ARB365</a>)"
                     /note="/Primary_Match=98.46%; Adjusted-Interval-0:0/0; 
                     SV40-PA
                     (<a href=""urn:local:.:a3k-bzmwvuk"">MTK0_037</a>)"
                     /note="/Transferred_From=MTK0_037"
                     /note="/Transferred_Similarity=98.46%"
     primer_bind     1859..1878
                     /label=KJ_T0091_SV40_Rev
     promoter        complement(1934..1952)
                     /label=M13_reverse_primer
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     M13_reverse_primer (<a 
                     href=""urn:local:.:a38-bzmwvuf"">MTK0_033</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     M13_reverse_primer (<a 
                     href=""urn:local:.:a3g-bzmwvuj"">MTK0_036</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     M13_reverse_primer (<a 
                     href=""urn:local:.:a24-bzmwvu0"">MTK0_004</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     M13_reverse_primer (<a 
                     href=""urn:local:.:a3o-bzmwvul"">mtk0_045</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0;
                     M13_reverse_primer (<a 
                     href=""urn:local:.:a2w-bzmwvub"">MTK0_028</a>)"
                     /note="/Transferred_From=MTK0_028"
                     /note="/Transferred_Similarity=100.00%"
     promoter        complement(1942..2016)
                     /label=trc
                     /note="hybrid of the trp and lac promoters that is
                     stronger than the lac promoter"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     trc
                     (<a href=""urn:local:.:eh-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     trc
                     (<a href=""urn:local:.:d-c1aa132"">SrtA-PDGFR Tm</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     trc
                     (<a href=""urn:local:.:2t-c1pusju"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     trc
                     (<a href=""urn:local:.:18-c1ldurm"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     trc
                     (<a href=""urn:local:.:1o-c0u8hgs"">SARS-CoV-2_S-2P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     trc
                     (<a href=""urn:local:.:y-c0v8hwh"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     trc
                     (<a href=""urn:local:.:eg-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     trc
                     (<a href=""urn:local:.:ef-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     trc
                     (<a href=""urn:local:.:ee-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; trc
                     (<a href=""urn:local:.:x-c34iv9l"">SARS-CoV-2_S-2P</a>)"
                     /note="/Transferred_From=SARS-CoV-2_S-2P"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=82.67%; Adjusted-Interval-0:0/0; <a
                     href=""""urn:local:.:1oh-bzc80e2"""">trc</a>"
                     /note="/X-Transferred_From=trc"
                     /note="/X-Transferred_Similarity=82.67%"
                     /note="/regulatory_class=promoter"
     misc_feature    1949..1966
                     /label=TTCCTGTGTGAAATTGTT
                     /label=nonstandard type: motif
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/%_Identity=100"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     TTCCTGTGTGAAATTGTT (<a 
                     href=""urn:local:.:dis-bzmypex"">pDE43-MCK</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     TTCCTGTGTGAAATTGTT (<a 
                     href=""urn:local:.:dhw-bzmypec"">pDE43-MCK</a>)"
                     /note="/Mismatches=0"
                     /note="/Motif=TTCCTGTGTGAAATTGTT"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0;
                     TTCCTGTGTGAAATTGTT (<a 
                     href=""urn:local:.:df2-bzmypaq"">pDE43-MCK</a>)"
                     /note="/Transferred_From=pDE43-MCK"
                     /note="/Transferred_Similarity=100.00%"
     protein_bind    1958..1982
                     /label=lac operator
                     /bound_moiety="<i>lac</i> repressor encoded by
                     <i>lacI</i>"
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli</i>. This 
                     inhibition can be relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=96.00%; Adjusted-Interval-0:0/0; 
                     lac
                     operator (<a href=""urn:local:.:18-bzpsocj"">PX408 
                     Francisella tularensis subsp. novicida Cas9</a>)"
                     /note="/Alternative_Match=96.00%; Adjusted-Interval-0:0/0; 
                     lac
                     operator (<a href=""urn:local:.:9hf-bzmwl6v"">Twin Strep 
                     SUMO_HA NLS Ec67</a>)"
                     /note="/Alternative_Match=96.00%; Adjusted-Interval-0:0/0; 
                     lac
                     operator (<a href=""urn:local:.:9hq-bzmwl70"">Twin Strep 
                     SUMO_NLS Ec86</a>)"
                     /note="/Alternative_Match=96.00%; Adjusted-Interval-0:0/0; 
                     lac
                     operator (<a href=""urn:local:.:9h6-bzmwl6t"">Twin Strep 
                     SUMO_Ec86</a>)"
                     /note="/Alternative_Match=96.00%; Adjusted-Interval-0:0/0; 
                     lac
                     operator (<a 
                     href=""urn:local:.:52-bzpstax"">TS-SUMO-AcrIIA11-LPETGG-3x 
                     HA (1)</a>)"
                     /note="/Alternative_Match=96.00%; Adjusted-Interval-0:0/0; 
                     lac
                     operator (<a 
                     href=""urn:local:.:9bh-bzmwcmh"">6XHIS_MBP_TEV_3XFLAG_SpyC 
                     as9-pMJ806</a>)"
                     /note="/Alternative_Match=96.00%; Adjusted-Interval-0:0/0; 
                     lac
                     operator (<a href=""urn:local:.:98g-bzmvycj"">PX408 
                     Francisella tularensis subsp. novicida Cas9</a>)"
                     /note="/Alternative_Match=96.00%; Adjusted-Interval-0:0/0; 
                     lac
                     operator (<a 
                     href=""urn:local:.:40-bzpstad"">AcrIIA11-6xHis</a>)"
                     /note="/Alternative_Match=96.00%; Adjusted-Interval-0:0/0; 
                     lac
                     operator (<a 
                     href=""urn:local:.:9a0-bzmvyde"">TS-SUMO-AcrIIA12-LPETGG-3 
                     xHA</a>)"
                     /note="/Primary_Match=96.00%; Adjusted-Interval-0:0/0; lac
                     operator (<a href=""urn:local:.:9hk-bzmwl6y"">Twin Strep 
                     SUMO_HA NLS Ec86</a>)"
                     /note="/Transferred_From=Twin Strep SUMO_HA NLS Ec86"
                     /note="/Transferred_Similarity=96.00%"
     misc_binding    complement(1960..1982)
                     /label=LacO
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     LacO
                     (<a href=""urn:local:.:eh-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     LacO
                     (<a href=""urn:local:.:d-c14z9bb"">nCoV_1_AVITag</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     LacO
                     (<a href=""urn:local:.:2t-c1pusju"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     LacO
                     (<a href=""urn:local:.:18-c1ldurm"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     LacO
                     (<a href=""urn:local:.:1o-c0u8hgs"">SARS-CoV-2_S-2P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     LacO
                     (<a href=""urn:local:.:y-c0v8hwh"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     LacO
                     (<a href=""urn:local:.:eg-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     LacO
                     (<a href=""urn:local:.:ef-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     LacO
                     (<a href=""urn:local:.:ee-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     LacO (<a 
                     href=""urn:local:.:x-c34iv9l"">SARS-CoV-2_S-2P</a>)"
                     /note="/Transferred_From=SARS-CoV-2_S-2P"
                     /note="/Transferred_Similarity=100.00%"
     misc_binding    1960..1982
                     /label=LacO
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     LacO (<a 
                     href=""urn:local:.:9bq-bzmwcmn"">His-TS-SUMO-SpCas9-SBP2</ 
                     a>)"
                     /note="/Transferred_From=His-TS-SUMO-SpCas9-SBP2"
                     /note="/Transferred_Similarity=100.00%"
     protein_bind    1962..1978
                     /label=lac operator
                     /bound_moiety="<i>lac</i> repressor encoded by
                     <i>lacI</i>"
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli</i>. This 
                     inhibition can be relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a href=""urn:local:.:9es-bzmwl64"">Jenny's 
                     MBP-RT purification vector</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:9bv-bzmwcmo"">Lenti_FnCas9_sfGFP 
                     DO</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:72-c0q55wp"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9 - KJ_T0505_IVT_gBlock_msr-msd_(Wt)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:9c9-bzmwcmt"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:9ea-bzmwl5z"">3XFLAG-NLS-RT</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:1l-c0q1vfs"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a href=""urn:local:.:96x-bzmvybt"">Pax2</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; lac
                     operator (<a 
                     href=""urn:local:.:9em-bzmwl63"">HA-NLS-RT-EGFP</a>)"
                     /note="/Transferred_From=HA-NLS-RT-EGFP"
                     /note="/Transferred_Similarity=100.00%"
     protein_bind    complement(1962..1978)
                     /label=lac operator
                     /bound_moiety="<html><body><i>lac</i> repressor encoded 
                     by<i>lacI</i></body></html>"
                     /note="<html><body>The <i>lac</i> repressor binds to the 
                     <i>lac</i> operator to inhibit transcription in <i>E. 
                     coli</i>. This inhibition can be relieved by adding lactose
                     or isopropyl-beta-D-thiogalactopyranoside 
                     (IPTG).</body></html>"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a href=""urn:local:.:3a-c2pgpvs"">backup of 
                     Jenny's_3XFLAG-MBP-RT(Eco2/Ec67)-NLS-8XHIS</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:h-c0h0i2n"">Jenny's_3XFLAG-MBP-RT(Eco2/ 
                     Ec67)-NLS-8XHIS</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:1e-bzrqycc"">Jenny's_RT_(Eco1/Ec86)</a> 
                     )"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:12-bzrqybz"">Jenny's_MBP-RT_(Eco5/Ec107 
                     )</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:f-bzrqj20"">Jenny's_3XFLAG-MBP-RT(Eco1/ 
                     Ec86)-NLS-8XHIS</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:y-bzrqybu"">Jenny's_MBP-RT_(Eco2/Ec67)< 
                     /a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:6j-c2wj415"">Jenny's_3XFLAG-MBP-RT(Eco1 
                     /Ec86)-NLS-8XHIS_purification_vector</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:2g-c1vsgt7"">Jenny's_3XFLAG-MBP-RT(Eco2 
                     /Ec67)-NLS-8XHIS</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:g-c21rl6b"">Jenny's_3XFLAG-MBP-RT(Eco5/ 
                     Ec107)-NLS-8XHIS</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; lac
                     operator (<a 
                     href=""urn:local:.:m-c0h0i8d"">Jenny's_3XFLAG-MBP-RT(Eco5/ 
                     Ec107)-NLS-8XHIS</a>)"
                     /note="/Transferred_From=Jenny's_3XFLAG-MBP-RT(Eco5/Ec107)-
                     NLS-8
                     XHIS"
                     /note="/Transferred_Similarity=100.00%"
     protein_bind    1962..1978
                     /label=lac operator
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a href=""urn:local:.:d-c1aa132"">SrtA-PDGFR 
                     Tm</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:7t-c2wj41q"">Lenti__IVT_gBlock_msr-msd_ 
                     (Wt)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:87-c2wj420"">Lenti_IVT_gBlock_msr-msd_( 
                     TurboRFP_repair)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:26-bzrqycr"">pIF508_msr-msd_(TurboRFP_r 
                     epair)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:8f-c2wj425"">Lenti_sgRNA_sfGFP-DO_no_Sp 
                     yCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:7z-c2wj41v"">Lenti_IVT_gBlock_msr-msd_( 
                     TurboRFP_repair)_HH_and_HDV</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:9ia-bzmwl77"">Exported</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:9b3-bzmvye1"">Exported</a>)"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/-5; lac
                     operator (<a 
                     href=""urn:local:.:51-c0q53l2"">KJ_T0507_IVT_gBlock_msr-ms 
                     d_(TurboRFP_repair)_HH_and_HDV</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; lac
                     operator (<a 
                     href=""urn:local:.:2c-bzrqycv"">pIF508_msr-msd_(TurboRFP_r 
                     epair)</a>)"
                     /note="/Transferred_From=pIF508_msr-msd_(TurboRFP_repair)"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    complement(1962..1978)
                     /label=Lac O1
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     Lac
                     O1 (<a href=""urn:local:.:af3-bzmyfmo"">pFF745</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     Lac
                     O1 (<a href=""urn:local:.:afe-bzmyfmt"">pFF745</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; Lac
                     O1
                     (<a href=""urn:local:.:aet-bzmyffh"">pFF745</a>)"
                     /note="/Transferred_From=pFF745"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    1962..1978
                     /label=lacO
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacO
                     (<a href=""urn:local:.:eh-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacO
                     (<a href=""urn:local:.:d-c1aa132"">SrtA-PDGFR Tm</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacO
                     (<a href=""urn:local:.:2t-c1pusju"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacO
                     (<a href=""urn:local:.:18-c1ldurm"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacO
                     (<a href=""urn:local:.:1o-c0u8hgs"">SARS-CoV-2_S-2P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacO
                     (<a href=""urn:local:.:y-c0v8hwh"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacO
                     (<a href=""urn:local:.:eg-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacO
                     (<a href=""urn:local:.:ef-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacO
                     (<a href=""urn:local:.:ee-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     lacO (<a 
                     href=""urn:local:.:x-c34iv9l"">SARS-CoV-2_S-2P</a>)"
                     /note="/Transferred_From=SARS-CoV-2_S-2P"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     <a href=""""urn:local:.:ph-bzc80ak"""">lacO</a>"
                     /note="/X-Transferred_From=lacO"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/kind=operator"
     misc_feature    1964..1978
                     /label=lacO
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on protein similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     lacO (<a href=""urn:local:.:37-c0ul19s"">SARS-CoV-2_S-2P 
                     translation 2</a>)"
                     /note="/Transferred_From=SARS-CoV-2_S-2P translation 2"
                     /note="/Transferred_Translation_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     <a href=""""urn:local:.:ph-bzc80ak"""">lacO</a>"
                     /note="/X-Transferred_From=lacO"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/kind=operator"
     misc_feature    1994..2005
                     /label=C
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on protein similarity"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     C
                     (<a href=""urn:local:.:eh-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     C
                     (<a href=""urn:local:.:d-c1aa132"">SrtA-PDGFR Tm</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     C
                     (<a href=""urn:local:.:2t-c1pusju"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     C
                     (<a href=""urn:local:.:18-c1ldurm"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     C
                     (<a href=""urn:local:.:1o-c0u8hgs"">SARS-CoV-2_S-2P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     C
                     (<a href=""urn:local:.:y-c0v8hwh"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     C
                     (<a href=""urn:local:.:eg-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     C
                     (<a href=""urn:local:.:ef-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     C
                     (<a href=""urn:local:.:ee-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Color=#FFFFB9"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; C 
                     (<a href=""urn:local:.:x-c34iv9l"">SARS-CoV-2_S-2P</a>)"
                     /note="/Transferred_From=SARS-CoV-2_S-2P"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     <a href=""""urn:local:.:te-bzc80b3"""">C</a>"
                     /note="/X-Transferred_From=C"
                     /note="/X-Transferred_Translation_Similarity=100.00%"
                     /note="/kind=tag"
     regulatory      2031..2052
                     /regulatory_class="other"
                     /label=CAP BS
                     /note="catabolite activator protein binding site"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     BS (<a href=""urn:local:.:eh-c30wztu"">SARS-CoV-2_S-2P_Cell
                     Surface 4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     BS (<a href=""urn:local:.:d-c1aa132"">SrtA-PDGFR Tm</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     BS (<a href=""urn:local:.:2t-c1pusju"">SARS-CoV-2_S-2P_Cell
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     BS (<a href=""urn:local:.:18-c1ldurm"">SARS-CoV-2_S-2P_Cell
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     BS (<a 
                     href=""urn:local:.:1o-c0u8hgs"">SARS-CoV-2_S-2P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     BS (<a href=""urn:local:.:y-c0v8hwh"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     BS (<a href=""urn:local:.:eg-c30wztu"">SARS-CoV-2_S-2P_Cell
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     BS (<a href=""urn:local:.:ef-c30wztu"">SARS-CoV-2_S-2P_Cell
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     BS (<a href=""urn:local:.:ee-c30wztu"">SARS-CoV-2_S-2P_Cell
                     Surface 1</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; CAP
                     BS
                     (<a href=""urn:local:.:x-c34iv9l"">SARS-CoV-2_S-2P</a>)"
                     /note="/Transferred_From=SARS-CoV-2_S-2P"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     <a href=""""urn:local:.:1v4-bzc80f3"""">CAP BS</a>"
                     /note="/X-Transferred_From=CAP BS"
                     /note="/X-Transferred_Similarity=100.00%"
     regulatory      2031..2052
                     /regulatory_class="other"
                     /standard_name="CAP BS"
                     /label=CAP BS
                     /note="catabolite activator protein binding site"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=95.45%; Adjusted-Interval-0:0/0; 
                     CAP
                     BS (<a href=""urn:local:.:11-c4pu1xm"">pMAZ-SK_795</a>)"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:2/-2; CAP
                     BS (<a href=""urn:local:.:37-c0ul19s"">SARS-CoV-2_S-2P 
                     translation 2</a>)"
                     /note="/Primary_Match=95.45%; Adjusted-Interval-0:0/0; CAP 
                     BS
                     (<a href=""urn:local:.:y-c4pu1xk"">pMAZ-SK_794</a>)"
                     /note="/Transferred_From=pMAZ-SK_794"
                     /note="/Transferred_Similarity=95.45%"
     protein_bind    2031..2052
                     /label=CAP binding site
                     /bound_moiety="<i>E. coli</i> catabolite activator
                     protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:9bv-bzmwcmo"">Lenti_FnCas9_sfGFP 
                     DO</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:72-c0q55wp"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9 - KJ_T0505_IVT_gBlock_msr-msd_(Wt)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:9c9-bzmwcmt"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:9ea-bzmwl5z"">3XFLAG-NLS-RT</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:1l-c0q1vfs"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:96x-bzmvybt"">Pax2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:v-c0u7dc2"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; CAP
                     binding site (<a 
                     href=""urn:local:.:9em-bzmwl63"">HA-NLS-RT-EGFP</a>)"
                     /note="/Transferred_From=HA-NLS-RT-EGFP"
                     /note="/Transferred_Similarity=100.00%"
     protein_bind    2031..2052
                     /label=CAP binding site
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a href=""urn:local:.:d-c1aa132"">SrtA-PDGFR 
                     Tm</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:7t-c2wj41q"">Lenti__IVT_gBlock_msr-msd_ 
                     (Wt)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:87-c2wj420"">Lenti_IVT_gBlock_msr-msd_( 
                     TurboRFP_repair)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:26-bzrqycr"">pIF508_msr-msd_(TurboRFP_r 
                     epair)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:8f-c2wj425"">Lenti_sgRNA_sfGFP-DO_no_Sp 
                     yCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:7z-c2wj41v"">Lenti_IVT_gBlock_msr-msd_( 
                     TurboRFP_repair)_HH_and_HDV</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:9ia-bzmwl77"">Exported</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:9b3-bzmvye1"">Exported</a>)"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:2/-2; CAP
                     binding site (<a 
                     href=""urn:local:.:37-c0ul19s"">SARS-CoV-2_S-2P translation
                     2</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; CAP
                     binding site (<a 
                     href=""urn:local:.:2c-bzrqycv"">pIF508_msr-msd_(TurboRFP_r 
                     epair)</a>)"
                     /note="/Transferred_From=pIF508_msr-msd_(TurboRFP_repair)"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    2159..2187
                     /label=RSF 1030 ori
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=96.55%; Adjusted-Interval-0:0/0; 
                     RSF
                     1030 ori (<a 
                     href=""urn:local:.:dqg-bzmyr4p"">pR6K_FRT_Kan</a>)"
                     /note="/Primary_Match=96.55%; Adjusted-Interval-0:0/0; RSF 
                     1030
                     ori (<a href=""urn:local:.:dtl-bzmyr9x"">pR6K_FRT_Kan</a>)"
                     /note="/Transferred_From=pR6K_FRT_Kan"
                     /note="/Transferred_Similarity=96.55%"
     unsure          2266..2305
                     /label=Spacer
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     Spacer (<a 
                     href=""urn:local:.:a4-c2wj42s"">pKJ0009:_ES.entry_-_Type_4 
                     _Spacer_(T0432_T0433)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     Spacer (<a 
                     href=""urn:local:.:9s-c2wj42n"">pKJ0007:_ES.entry_-_Type_2 
                     _Spacer_(T0428_T0429)</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Spacer
                     (<a 
                     href=""urn:local:.:9y-c2wj42p"">pKJ0008:_ES.entry_-_Type_3 
                     _Spacer_(T0430_T0431)</a>)"
                     /note="/Transferred_From=pKJ0008:_ES.entry_-_Type_3_Spacer_
                     (T043
                     0_T0431)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=moham"
     primer_bind     complement(2280..2296)
                     /label=KJ_T0040_ColE1_Fwd
     misc_feature    2325..2944
                     /label=pBR322
                     /label=nonstandard type: ori
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=99.84%; Adjusted-Interval-0:0/0; 
                     pBR322
                     (<a href=""urn:local:.:x-c8vvcds"">pKAR2</a>)"
                     /note="/Transferred_From=pKAR2"
                     /note="/Transferred_Similarity=99.84%"
                     /note="/created_by=User"
     rep_origin      2340..2928
                     /label=ori
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; ori
                     (<a href=""urn:local:.:w-bzpsocc"">EZeo vector_Simon</a>)"
                     /note="/Transferred_From=EZeo vector_Simon"
                     /note="/Transferred_Similarity=100.00%"
     misc_RNA        complement(2556..2585)
                     /label=crRNA-ColA Ori
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=83.33%; Adjusted-Interval-0:0/0;
                     crRNA-ColA Ori (<a 
                     href=""urn:local:.:i-c21rl6b"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Alternative_Match=83.33%; Adjusted-Interval-0:0/0;
                     crRNA-ColA Ori (<a 
                     href=""urn:local:.:2u-c1vshzl"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Alternative_Match=83.33%; Adjusted-Interval-0:0/0;
                     crRNA-ColA Ori (<a 
                     href=""urn:local:.:y-c4pu1xk"">pMAZ-SK_794</a>)"
                     /note="/Alternative_Match=83.33%; Adjusted-Interval-0:0/0;
                     crRNA-ColA Ori (<a 
                     href=""urn:local:.:11-c4pu1xm"">pMAZ-SK_795</a>)"
                     /note="/Alternative_Match=83.33%; Adjusted-Interval-0:0/0;
                     crRNA-ColA Ori (<a 
                     href=""urn:local:.:85-c5ca8r3"">pMAZ-SK</a>)"
                     /note="/Alternative_Match=83.33%; Adjusted-Interval-0:0/0;
                     crRNA-ColA Ori (<a 
                     href=""urn:local:.:2f-c0hk8ws"">pMAZ-SK</a>)"
                     /note="/Primary_Match=83.33%; Adjusted-Interval-0:0/0;
                     crRNA-ColA Ori (<a 
                     href=""urn:local:.:1zc-bzlzoz3"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Transferred_From=pMAZ-SK_sfGFP DO"
                     /note="/Transferred_Similarity=83.33%"
                     /note="/created_by=moham"
                     /note="/modified_by=moham"
     misc_feature    complement(2945..3003)
                     /label=pMB1 region
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     pMB1
                     region (<a href=""urn:local:.:x-c8vvcds"">pKAR2</a>)"
                     /note="/Transferred_From=pKAR2"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_difference 2950..2967
                     /label=KanF_seq
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/%_Identity=100"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     KanF_seq (<a 
                     href=""urn:local:.:dpm-bzmyqla"">kanRnullwithSpec</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     KanF_seq (<a 
                     href=""urn:local:.:d2l-bzmyo2r"">kanRnullwithSpec</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     KanF_seq (<a 
                     href=""urn:local:.:ckv-bzmynor"">kanRnullwithSpec</a>)"
                     /note="/Motif=CTGACGCTCAG"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     KanF_seq
                     (<a href=""urn:local:.:dp0-bzmyqkg"">kanRnullwithSpec</a>)"
                     /note="/Transferred_From=kanRnullwithSpec"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/modified_by=User"
     primer_bind     complement(2979..3000)
                     /label=KJ_T0018_Ori_Rev
     misc_feature    2983..3003
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0;  
                     (<a href=""urn:local:.:x-c8vvcds"">pKAR2</a>)"
                     /note="/Transferred_From=pKAR2"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     complement(2984..3014)
                     /label=KJ_T0470_Ec86_Beta_Backbone_rev
     primer_bind     2995..3033
                     /label=KJ_T0471_AmpR_Insert_fwd
     misc_feature    3000..3021
                     /label=Geneious type: structural annotation
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Geneious type: structural annotation"
                     /note="/Primary_Match=81.82%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:.:aq-c2wk6f6"">TU3ER</a>)"
                     /note="/Transferred_From=TU3ER"
                     /note="/Transferred_Similarity=81.82%"
                     /note="/created_by=User"
     terminator      complement(3005..3101)
                     /label=AmpR Terminator
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Terminator (<a 
                     href=""urn:local:.:a34-bzmwvue"">MTK0_031</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Terminator (<a 
                     href=""urn:local:.:9ik-bzmwsep"">MTK0_001</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Terminator (<a 
                     href=""urn:local:.:9kc-bzmwsfg"">MTK0_025</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Terminator (<a 
                     href=""urn:local:.:9x4-bzmwvr9"">ARB343</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Terminator (<a 
                     href=""urn:local:.:9xk-bzmwvrf"">ARB347</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Terminator (<a 
                     href=""urn:local:.:9jw-bzmwsfa"">MTK0_021</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Terminator (<a href=""urn:local:.:aq-c2wk6f6"">TU3ER</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Terminator (<a 
                     href=""urn:local:.:92g-bzmvb6x"">pYTK095</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Terminator (<a 
                     href=""urn:local:.:9x8-bzmwvrb"">ARB344</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR
                     Terminator (<a 
                     href=""urn:local:.:9ww-bzmwvr7"">ARB341</a>)"
                     /note="/Transferred_From=ARB341"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    complement(3012..3060)
                     /label=terminator
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     terminator (<a 
                     href=""urn:local:.:6g-c4ce7j9"">P114-R5-GfOMT1_2.4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     terminator (<a 
                     href=""urn:local:.:d-c3b6zke"">P114-R5-GfOMT1_1.6</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     terminator (<a 
                     href=""urn:local:.:h-c3b6zrl"">P114-R5-GfOMT1_2.4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     terminator (<a 
                     href=""urn:local:.:o-c3b76y3"">P114-R5-GfOMT1_2.4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     terminator (<a 
                     href=""urn:local:.:n-c3b76y3"">P114-R5-GfOMT1_1.6</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0;
                     terminator (<a 
                     href=""urn:local:.:6f-c4ce7j9"">P114-R5-GfOMT1_1.6</a>)"
                     /note="/Transferred_From=P114-R5-GfOMT1_1.6"
                     /note="/Transferred_Similarity=100.00%"
     CDS             complement(3099..3959)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (<a 
                     href=""urn:local:.:2c-bzrqycv"">pIF508_msr-msd_(TurboRFP_r 
                     epair)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (<a 
                     href=""urn:local:.:y-c2vthvs"">pDisplay-mSA-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (<a href=""urn:local:.:d-c1aa132"">SrtA-PDGFR Tm</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (<a 
                     href=""urn:local:.:7n-c8vy8oc"">pDisplay-mSA-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (<a href=""urn:local:.:1y-c61zrev"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (<a 
                     href=""urn:local:.:26-bzrqycr"">pIF508_msr-msd_(TurboRFP_r 
                     epair)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (<a href=""urn:local:.:1x-c61zreu"">pDisplay-SS47 
                     (86)-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (<a href=""urn:local:.:1x-c7wp0k7"">pDisplay-SS47 
                     (86)-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (<a href=""urn:local:.:19-c5kw4c2"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR (<a href=""urn:local:.:6a-c8vxg0z"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)"
                     /note="/Transferred_From=pDisplay-SS47 (87)-EGFP-TM"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=MSIQHFRVALIPFFAAFCLPVFAHPET
                     LVKVK 
                     DAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLG 
                     RRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTA 
                     FLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLI 
                     DWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGS 
                     QATMDERNRQIAEIGASLIKHW"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(3239..3244)
                     /label=BsaI
                     /note="Geneious type: restriction site"
                     /note="/Methylation=Dcm:variable, CpG:variable, EcoBI:cut"
                     /note="/Recognition_pattern=GGTCTC(1/5)"
                     /note="/annotation_group=GGTCTC"
     promoter        complement(3960..4090)
                     /label=AmpR Promoter
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Promoter (<a 
                     href=""urn:local:.:a34-bzmwvue"">MTK0_031</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Promoter (<a 
                     href=""urn:local:.:9ik-bzmwsep"">MTK0_001</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Promoter (<a 
                     href=""urn:local:.:9kc-bzmwsfg"">MTK0_025</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Promoter (<a href=""urn:local:.:9x4-bzmwvr9"">ARB343</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Promoter (<a href=""urn:local:.:9xk-bzmwvrf"">ARB347</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Promoter (<a 
                     href=""urn:local:.:9jw-bzmwsfa"">MTK0_021</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Promoter (<a href=""urn:local:.:aq-c2wk6f6"">TU3ER</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Promoter (<a href=""urn:local:.:92g-bzmvb6x"">pYTK095</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Promoter (<a href=""urn:local:.:9x8-bzmwvrb"">ARB344</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR
                     Promoter (<a href=""urn:local:.:9ww-bzmwvr7"">ARB341</a>)"
                     /note="/Transferred_From=ARB341"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    complement(4001..4029)
                     /label=AmpR promoter
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (<a 
                     href=""urn:local:.:6g-c4ce7j9"">P114-R5-GfOMT1_2.4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (<a 
                     href=""urn:local:.:d-c3b6zke"">P114-R5-GfOMT1_1.6</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (<a 
                     href=""urn:local:.:h-c3b6zrl"">P114-R5-GfOMT1_2.4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (<a 
                     href=""urn:local:.:o-c3b76y3"">P114-R5-GfOMT1_2.4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (<a 
                     href=""urn:local:.:n-c3b76y3"">P114-R5-GfOMT1_1.6</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR
                     promoter (<a 
                     href=""urn:local:.:6f-c4ce7j9"">P114-R5-GfOMT1_1.6</a>)"
                     /note="/Transferred_From=P114-R5-GfOMT1_1.6"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    4007..4023
                     /label=lacO
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     lacO (<a href=""urn:local:.:d-c1aa132"">SrtA-PDGFR Tm</a>)"
                     /note="/Transferred_From=SrtA-PDGFR Tm"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=70.59%; Adjusted-Interval-0:0/0; <a
                     href=""""urn:local:.:ph-bzc80ak"""">lacO</a>"
                     /note="/X-Transferred_From=lacO"
                     /note="/X-Transferred_Similarity=70.59%"
                     /note="/kind=operator"
     polyA_site      complement(4008..4037)
                     /label=Spec_F
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Spec_F
                     (<a href=""urn:local:.:d1q-bzmyo2k"">kanRnullwithSpec</a>)"
                     /note="/Transferred_From=kanRnullwithSpec"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     4294..4308
                     /label=KJ_T0021_CMV_Fwd
     primer_bind     complement(4313..4329)
                     /label=KJ_T0156_CMV_Rev
     enhancer        4330..4709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     enhancer (<a 
                     href=""urn:local:.:9bv-bzmwcmo"">Lenti_FnCas9_sfGFP 
                     DO</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     enhancer (<a 
                     href=""urn:local:.:72-c0q55wp"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9 - KJ_T0505_IVT_gBlock_msr-msd_(Wt)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     enhancer (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     enhancer (<a 
                     href=""urn:local:.:9c9-bzmwcmt"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     enhancer (<a 
                     href=""urn:local:.:9ea-bzmwl5z"">3XFLAG-NLS-RT</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     enhancer (<a 
                     href=""urn:local:.:1l-c0q1vfs"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     enhancer (<a href=""urn:local:.:96x-bzmvybt"">Pax2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     enhancer (<a 
                     href=""urn:local:.:9e7-bzmwl5y"">3XFLAG-NLS-RT-EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     enhancer (<a 
                     href=""urn:local:.:973-bzmvybu"">pcDNA-FRT-TO-intron</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; CMV
                     enhancer (<a 
                     href=""urn:local:.:9em-bzmwl63"">HA-NLS-RT-EGFP</a>)"
                     /note="/Transferred_From=HA-NLS-RT-EGFP"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     4462..4481
                     /label=KJ_T0234_pAAV_CMV_Fwd
     promoter        4710..4913
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     promoter (<a href=""urn:local:.:95r-bzmvybf"">mNeptune2 
                     CRISPEY msr msd</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     promoter (<a 
                     href=""urn:local:.:9av-bzmvydw"">Exported</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     promoter (<a href=""urn:local:.:95x-bzmvybg"">mNeptune2 
                     CRISPEY_81mer_sgRNA1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     promoter (<a href=""urn:local:.:6a-c8vxg0z"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     promoter (<a 
                     href=""urn:local:.:9ea-bzmwl5z"">3XFLAG-NLS-RT</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     promoter (<a href=""urn:local:.:96x-bzmvybt"">Pax2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     promoter (<a href=""urn:local:.:18-bzpsocj"">PX408 
                     Francisella tularensis subsp. novicida Cas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     promoter (<a 
                     href=""urn:local:.:9e7-bzmwl5y"">3XFLAG-NLS-RT-EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     promoter (<a 
                     href=""urn:local:.:973-bzmvybu"">pcDNA-FRT-TO-intron</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; CMV
                     promoter (<a 
                     href=""urn:local:.:9em-bzmwl63"">HA-NLS-RT-EGFP</a>)"
                     /note="/Transferred_From=HA-NLS-RT-EGFP"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     4888..4907
                     /label=KJ_T0196_CMV_Fwd
     protein_bind    join(4915..4933,4936..4954)
                     /label=TetO
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:1z8-bzlzoz0"">pMA7CR_2.0</a>)"
                     /note="/Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:1z8-bzlzoz0"">pMA7CR_2.0</a>)"
                     /note="/Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:1zc-bzlzoz3"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:1zc-bzlzoz3"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:1zc-bzlzoz3"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:1zc-bzlzoz3"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:i-c21rl6b"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:i-c21rl6b"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:i-c21rl6b"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0, 
                     Adjusted-Interval-1:0/0; TetO (<a 
                     href=""urn:local:.:ai-c2wk6ez"">pSK.102</a>)"
                     /note="/Transferred_From=pSK.102"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     protein_bind    4915..4933
                     /gene="<i>tetO</i>"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O<sub>2</sub> for the
                     <i>tetR</i> and <i>tetA</i> genes"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Transferred_From=Lenti_TET_HA_NLS_EcoI_EGFP"
                     /note="/Transferred_Similarity=100.00%"
     protein_bind    4936..4954
                     /gene="<i>tetO</i>"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O<sub>2</sub> for the
                     <i>tetR</i> and <i>tetA</i> genes"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Transferred_From=Lenti_TET_HA_NLS_EcoI_EGFP"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     4957..4974
                     /label=KJ_T0158_CMV_Fwd
     primer_bind     4972..4989
                     /label=KJ_T0010_AcGFP1 Seq_Fwd
     misc_feature    complement(4994..4999)
                     /label=Esp3I, BsmBI
                     /note="Geneious type: restriction site"
                     /note="/Methylation=Mixed Methylation"
                     /note="/Recognition_pattern=CGTCTC(1/5)"
                     /note="/annotation_group=CGTCTC"
     primer_bind     5033..5047
                     /label=KJ_T0024_TurboRFP_Fwd
     intron          5107..5239
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     chimeric intron (<a 
                     href=""urn:local:.:9b3-bzmvye1"">Exported</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     chimeric
                     intron (<a href=""urn:local:.:9ia-bzmwl77"">Exported</a>)"
                     /note="/Transferred_From=Exported"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    complement(5138..5143)
                     /label=BsaI
                     /note="Geneious type: restriction site"
                     /note="/Methylation=Dcm:variable, CpG:variable, EcoBI:cut"
                     /note="/Recognition_pattern=GGTCTC(1/5)"
                     /note="/annotation_group=GGTCTC"
     promoter        5284..5302
                     /label=T7 Promoter
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     T7
                     Promoter (<a 
                     href=""urn:local:.:9m-c2wj42l"">pKJ0006:_ES.entry_-_T7_Pro 
                     moter_RBS1_(T0434_T0435)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     T7
                     Promoter (<a href=""urn:local:.:8n-c2wj429"">pKJ0001</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     T7
                     Promoter (<a 
                     href=""urn:local:.:6y-c2wj41d"">KJ_T0417_msr_Eco1_</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     T7
                     promoter (<a 
                     href=""urn:local:.:9em-bzmwl63"">HA-NLS-RT-EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     T7
                     promoter (<a 
                     href=""urn:local:.:1e-c4vgcq6"">KJ_T0630_IVT_gBlock_msr-ms 
                     d RFP repair </a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     T7
                     promoter (<a href=""urn:local:.:9hk-bzmwl6y"">Twin Strep 
                     SUMO_HA NLS Ec86</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     T7
                     promoter (<a 
                     href=""urn:local:.:97w-bzmvyc8"">pMBP-AsCas12a</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     T7
                     promoter (<a 
                     href=""urn:local:.:52-c0q53l2"">KJ_T0518_IVT_gBlock_msr-ms 
                     d_(Wt)_mutant_1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     T7
                     promoter (<a href=""urn:local:.:6a-c8vxg0z"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; T7
                     Promoter (<a 
                     href=""urn:local:.:6r-c2wj41b"">KJ_T0417_msr_Eco1__-_mScar 
                     let-DO_(T0417/T0418)_-_KJ_T0418_msd_Eco1_</a>)"
                     /note="/Transferred_From=T7 Promoter"
                     /note="/Transferred_From=KJ_T0417_msr_Eco1__-_mScarlet-DO_(
                     T0417 /T0418)_-_KJ_T0418_msd_Eco1_"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=moham"
     primer_bind     complement(5329..5357)
                     
     primer_bind     complement(5330..5347)
                     /label=KJ_T0045_pcDNA5_NotI_Rev
     primer_bind     5338..5368
                     
     CDS             5348..5410
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     misc_feature    5407..5410
                     /label=Part1 5' Overhang
                     /label=nonstandard type: overhang
                     /note="/created_by=moham"
     misc_feature    complement(5415..5421)
                     /label=AarI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CACCTGC(4/8)"
                     /note="/annotation_group=CACCTGC"
     promoter        5422..5551
                     /label=BBa_J72163 GlpT Promoter
     misc_feature    5552..5587
                     /label=sfGFP Ribosome Binding Site
     RBS             5570..5581
                     /label=RBS
                     /note="strong bacterial ribosome binding site (<a 
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/10659856"" 
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/10659856"">Elow 
                     itz and Leibler, 2000</a>)"
     CDS             5588..6301
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused
                     to poorly folded proteins (<a 
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/16369541"" 
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/16369541"">Pede 
                     lacq et al., 2006</a>)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      6313..6384
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the <i>E. coli
                     rrnB</i> gene"
     terminator      6400..6427
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    6434..6440
                     /label=AarI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CACCTGC(4/8)"
                     /note="/annotation_group=CACCTGC"
     CDS             6445..9271
                     /codon_start=2
                     /label=SARS-CoV-2 Spike Protein
                     /note="/Original_Translation=MFVFLVLLPLVSSQCVNLTTRTQLPPAYTN
                     SFTRG 
                     VYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFA 
                     STEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKS 
                     WMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTP 
                     INLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYV 
                     GYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTES 
                     IVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVS 
                     PTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNL 
                     DSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQP 
                     TNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNK 
                     KFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDV 
                     NCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICAS 
                     YQTQTNSPGSASSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSM 
                     TKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIY 
                     KTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAAR 
                     DLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYR 
                     FNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLV 
                     KQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRAS 
                     ANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAP 
                     AICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTV 
                     YDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNE 
                     SLIDLQELGKYEQ"
                     /note="/modified_by=moham"
                     /translation="VGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKG
                     IYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYN
                     SASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDF
                     TGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNC
                     YFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLT
                     GTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSN
                     QVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIP
                     IGAGICASYQTQTNSPGSASSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTT
                     EILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFA
                     QVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLG
                     DIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQ
                     MAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALN
                     TLVKQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIR
                     ASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTA
                     PAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTV
                     YDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNES
                     LIDLQELGKYEQ"
     gene            6445..6562
                     /label=NTD
                     /note="/created_by=moham"
     misc_feature    6445..6448
                     /label=Part1 3' Overhang
                     /label=nonstandard type: overhang
                     /note="/created_by=moham"
     misc_feature    6445..6448
                     /label=Part2 5' Overhang
                     /label=nonstandard type: overhang
                     /note="/created_by=moham"
     gene            6563..7210
                     /label=RBD
                     /note="/created_by=moham"
     gene            7211..8368
                     /label=FP
                     /note="/created_by=moham"
     misc_feature    7211..7214
                     /label=Part2 3' Overhang
                     /label=nonstandard type: overhang
                     /note="/created_by=moham"
     gene            8370..9271
                     /label=HR, CH, and CTD
                     /note="/created_by=moham"
     misc_feature    9268..9271
                     /label=3' overhang
                     /label=nonstandard type: overhang
                     /note="/created_by=moham"
     misc_feature    9281..9361
                     /label=Foldon
     CDS             9368..9433
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             9440..9463
                     /label=Strep Tag II
                     /note="/created_by=moham"
     CDS             9470..9493
                     /label=PreScission site (3C)
                     /note="/created_by=moham"
                     /note="/modified_by=moham"
     gene            9536..9682
                     /label=PDGFR-B TM domain
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     PDGFR-B TM domain (<a 
                     href=""urn:local:.:2t-c1pusju"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     PDGFR-B TM domain (<a 
                     href=""urn:local:.:18-c1ldurm"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     PDGFR-B TM domain (<a 
                     href=""urn:local:.:y-c0v8hwh"">SARS-CoV-2_S-2P_Cell Surface
                     1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     PDGFR-B TM domain (<a 
                     href=""urn:local:.:eg-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     PDGFR-B TM domain (<a 
                     href=""urn:local:.:ef-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     PDGFR-B TM domain (<a 
                     href=""urn:local:.:ee-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     PDGFR-B TM domain (<a 
                     href=""urn:local:.:4y-c1pwmyb"">SARS-CoV-2_S-2P_Cell 
                     Surface 4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     PDGFR-B TM domain (<a 
                     href=""urn:local:.:h-c9zxg2l"">Spike-hinge-TM extract</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     PDGFR-B TM domain (<a 
                     href=""urn:local:.:n-c79snqp"">S-2P_86_TM</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     PDGFR-B
                     TM domain (<a 
                     href=""urn:local:.:eh-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 4</a>)"
                     /note="/Transferred_From=SARS-CoV-2_S-2P_Cell Surface 4"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=moham"
ORIGIN
        1 taacggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
       61 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
      121 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
      181 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
      241 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
      301 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      361 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
      421 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
      481 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
      541 ggtgatggtt cacgtaccta gaagttccta ttccgaagtt cctattctct agaaagtata
      601 ggaacttcct tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
      661 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
      721 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
      781 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
      841 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
      901 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
      961 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     1021 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     1081 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     1141 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     1201 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     1261 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     1321 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     1381 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     1441 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     1501 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     1561 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     1621 ccgagggcaa aggaatagca cgtactacga gatttcgatt ccaccgccgc cttctatgaa
     1681 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     1741 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     1801 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     1861 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     1921 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     1981 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2041 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2101 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2161 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2221 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2281 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2341 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2401 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2461 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2521 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2581 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2641 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2701 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2761 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2821 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2881 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2941 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3001 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3061 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3121 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3181 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3241 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3301 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3361 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3421 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3481 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3541 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3601 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3661 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3721 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3781 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3841 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3901 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3961 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4021 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4081 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     4141 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     4201 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     4261 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     4321 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     4381 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     4441 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     4501 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     4561 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     4621 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     4681 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     4741 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     4801 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     4861 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta
     4921 tcagtgatag agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc
     4981 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
     5041 gatccagcct ccggactcta gcgtttaaac ttaagctgca gaagttggtc gtgaggcact
     5101 gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg
     5161 tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac
     5221 tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta aggctagagt
     5281 acttaatacg actcactata ggctagcctc gagaattcac gcgtggtacc tctagagtcg
     5341 acccgggatg gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc
     5401 cactggtgac ggtggcaggt ggaaagtgaa acgtgatttc atgcgtcatt ttgaacattt
     5461 tgtaaatctt atttaataat gtgtgcggca attcacattt aatttatgaa tgttttctta
     5521 acatcgcggc aactcaagaa acggcaggtt cggatcttag ctactagaga aagaggagaa
     5581 atactagatg cgtaaaggcg aagagctgtt cactggtgtc gtccctattc tggtggaact
     5641 ggatggtgat gtcaacggtc ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac
     5701 taatggtaaa ctgacgctga agttcatctg tactactggt aaactgccgg ttccttggcc
     5761 gactctggta acgacgctga cttatggtgt tcagtgcttt gctcgttatc cggaccatat
     5821 gaagcagcat gacttcttca agtccgccat gccggaaggc tatgtgcagg aacgcacgat
     5881 ttcctttaag gatgacggca cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac
     5941 cctggtaaac cgcattgagc tgaaaggcat tgactttaaa gaggacggca atatcctggg
     6001 ccataagctg gaatacaatt ttaacagcca caatgtttac atcaccgccg ataaacaaaa
     6061 aaatggcatt aaagcgaatt ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct
     6121 ggctgatcac taccagcaaa acactccaat cggtgatggt cctgttctgc tgccagacaa
     6181 tcactatctg agcacgcaaa gcgttctgtc taaagatccg aacgagaaac gcgatcatat
     6241 ggttctgctg gagttcgtaa ccgcagcggg catcacgcat ggtatggatg aactgtacaa
     6301 atgaccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     6361 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     6421 ttctgcgttt atacacctgc cacctgtggg ctacctgcaa cctagaacct tcctgctgaa
     6481 gtacaacgag aacggcacaa tcacagacgc cgtcgactgc gccctggacc ctctctctga
     6541 gacaaagtgc accctgaagt ccttcaccgt ggaaaagggc atctaccaga ccagcaactt
     6601 ccgggtgcag cctacagaga gcatcgtgcg atttccaaac attaccaacc tctgcccctt
     6661 cggcgaggtg tttaacgcca caagatttgc ctccgtttac gcctggaata gaaagagaat
     6721 cagcaattgt gtggccgact actccgtgct gtataacagc gcctctttca gcaccttcaa
     6781 gtgctacggc gtttccccaa caaagctgaa tgacctgtgc ttcaccaacg tgtacgccga
     6841 ctccttcgta attagaggcg atgaggtgcg gcagatcgca ccaggccaga ccggtaagat
     6901 cgctgactac aactataagc tgcctgatga ttttacaggc tgcgtgatcg cctggaactc
     6961 taacaacctg gatagcaagg tgggcggcaa ctacaactac ctgtaccggc tgtttcgcaa
     7021 gtctaacctg aaacctttcg agagagacat ctccacagag atctaccagg ccggttctac
     7081 accttgtaac ggggtggaag gcttcaactg ttacttccct ctgcaaagct acggcttcca
     7141 gcctaccaat ggagtcggct accagccata ccgggtggtc gtgctgtcct tcgagttact
     7201 ccacgccccc gccaccgtct gcggtcctaa gaagtccacc aatctggtta agaacaaatg
     7261 cgtgaacttc aacttcaacg gcctgaccgg gaccggcgtg ctgaccgaaa gcaacaaaaa
     7321 gttcctcccc ttccagcagt tcggccgtga tatcgctgac accacagatg ccgtcagaga
     7381 tccacagacc ctggaaatcc tggatattac accctgctcc ttcggaggag tttctgtgat
     7441 cacccccggg accaatacca gcaaccaggt ggctgtgctg taccaagatg ttaactgcac
     7501 cgaggttcct gtggccatcc acgccgatca gctgacacct acttggagag tgtactccac
     7561 tggctccaat gtgttccaga ccagggccgg atgtctgatc ggcgccgagc acgtgaataa
     7621 cagttacgag tgcgacatcc ctatcggcgc cggcatctgt gccagctacc agacccagac
     7681 aaacagccct gggtctgctt cctctgtagc tagccagagc atcatcgcct acaccatgag
     7741 cctgggcgca gagaacagcg tggcctattc caacaactct atcgccattc ccaccaactt
     7801 tacaattagc gtcacaacag agatcctgcc cgtgagcatg accaagacca gcgtggactg
     7861 tacaatgtac atctgtggcg acagcactga atgcagcaac ctgctgctgc aatacggctc
     7921 cttttgcacc caactgaacc gggcgctgac cggaatcgcc gtggaacagg acaaaaatac
     7981 ccaggaggtg ttcgcccaag tgaagcagat ctacaagacc ccacctatca aggacttcgg
     8041 cggctttaac tttagccaga ttctccctga tccttctaag cctagcaagc ggagctttat
     8101 cgaggatctg ctgttcaaca aggtcaccct ggccgatgcc ggctttatca aacagtatgg
     8161 cgattgcctg ggcgacatag ccgccagaga tctgatctgc gcccagaaat tcaacggcct
     8221 gacagttctc ccacctctgc tgaccgacga gatgatcgct cagtacacct ctgccctgct
     8281 ggctggcacc atcacatctg ggtggacatt tggcgccggc gccgccctgc agatcccctt
     8341 tgccatgcag atggcctata gattcaacgg aatcggcgtg acccagaacg tgctgtatga
     8401 aaaccagaag ctgatcgcta accagttcaa ttctgccatc ggcaagatcc aggactccct
     8461 ctcctctacc gccagcgccc tgggcaaact gcaggacgtg gtgaatcaga acgcccaagc
     8521 cctgaacacc ctggtgaagc agctcagcag caattttggc gccatcagct ctgtgctgaa
     8581 cgatatcctg tctagactgg accctccaga agccgaagtc cagatcgata gactgatcac
     8641 aggcagactg cagtccctgc aaacctacgt gacccaacag ctgatcaggg ccgctgaaat
     8701 aagagccagc gccaatctcg ccgctaccaa gatgtccgag tgtgtgctgg gacagtctaa
     8761 acgcgttgac ttctgcggca aaggctatca cctgatgagc ttcccccaga gcgcgccgca
     8821 cggcgtggtg ttcctgcatg tgacatacgt gcctgcccaa gagaagaatt tcacaaccgc
     8881 ccctgccatc tgccacgacg gcaaggccca cttcccaaga gagggcgttt tcgtttccaa
     8941 tggcacacac tggttcgtga cacaaagaaa cttctacgaa ccccagatta tcaccaccga
     9001 caacaccttc gtgagtggca attgtgacgt ggtcatcgga atcgtgaaca acacagtgta
     9061 cgaccctctg caacctgagc tggactcttt taaggaagag ctggacaagt actttaaaaa
     9121 ccacaccagc cccgatgtgg acctgggcga catcagtggc attaacgcca gcgtggtgaa
     9181 catccaaaag gaaatcgaca gactgaacga ggtggccaag aacctgaacg agtccctgat
     9241 cgacctgcag gagctcggca aatacgagca gggatccgga tacatccccg aggcccccag
     9301 agatggccag gcctacgtgc ggaaggacgg cgagtgggta ctgctgagca cattcctggg
     9361 cagatccgac tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga
     9421 tgacgatgac aagagcgcct ggtcccaccc ccagttcgag aagggatccc tggaggtgct
     9481 gttccagggc ccaggcggcg gcggtagtgg agggggcgga tctggcggct cagctgctgt
     9541 gggccaggac acgcaggagg tcatcgtggt gccacactcc ttgcccttta aggtggtggt
     9601 gatctcagcc atcctggccc tggtggtgct caccatcatc tcccttatca tcctcatcat
     9661 gctttggcag aagaagccac gt
//
