LOCUS       Exported               10023 bp DNA     circular SYN 22-JUL-2021
DEFINITION  sfGFP DO for Part 5.
ACCESSION   urn.local...vj-dn74pn0
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 17, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10023
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          5411..6207
                     /label=Part 1
                     /note="/created_by=moham"
                     /note="/modified_by=moham"
     source          5411..5816
                     /label=Part 1a
                     /note="/created_by=moham"
     source          5821..6207
                     /label=Part 1b
                     /note="/created_by=moham"
     source          6212..6973
                     /label=Part 2
                     /note="/created_by=moham"
     source          6978..7770
                     /label=Part 3
                     /note="/created_by=moham"
     source          7775..8569
                     /label=Part 4
                     /note="/created_by=moham"
     source          8574..9607
                     /label=Part 5 DO
                     /note="/created_by=moham"
                     /note="/modified_by=moham"
     primer_bind     4..20
                     /label=KJ_T0046_pcDNA5_NotI_Fwd
     primer_bind     5..20
                     /label=KJ_T0399_FLAG_Cterm_Fwd
     primer_bind     complement(37..52)
                     /label=KJ_T0020_EGFP_Rev
     terminator      42..277
                     /label=BGH-PA
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=98.31%; Adjusted-Interval-0:0/0;
                     BGH-PA (<a href=""urn:local:.:9zo-bzmwvsn"">JPF0509</a>)"
                     /note="/Alternative_Match=98.31%; Adjusted-Interval-0:0/0;
                     BGH-PA (<a href=""urn:local:.:9ww-bzmwvr7"">ARB341</a>)"
                     /note="/Alternative_Match=98.31%; Adjusted-Interval-0:0/0;
                     BGH-PA (<a href=""urn:local:.:9yc-bzmwvrt"">ARB367</a>)"
                     /note="/Alternative_Match=98.31%; Adjusted-Interval-0:0/0;
                     BGH-PA (<a href=""urn:local:.:9yo-bzmwvs4"">ARB387</a>)"
                     /note="/Alternative_Match=98.31%; Adjusted-Interval-0:0/0;
                     BGH-PA (<a href=""urn:local:.:9x4-bzmwvr9"">ARB343</a>)"
                     /note="/Alternative_Match=98.31%; Adjusted-Interval-0:0/0;
                     BGH-PA (<a href=""urn:local:.:a1s-bzmwvtt"">JPF0536b</a>)"
                     /note="/Alternative_Match=98.31%; Adjusted-Interval-0:0/0;
                     BGH-PA (<a href=""urn:local:.:a10-bzmwvtc"">JPF0521</a>)"
                     /note="/Alternative_Match=98.31%; Adjusted-Interval-0:0/0;
                     BGH-PA (<a href=""urn:local:.:a0g-bzmwvt2"">JPF0516</a>)"
                     /note="/Alternative_Match=98.31%; Adjusted-Interval-0:0/0;
                     BGH-PA (<a href=""urn:local:.:9y8-bzmwvrr"">ARB366</a>)"
                     /note="/Primary_Match=98.31%; Adjusted-Interval-0:0/0; 
                     BGH-PA
                     (<a href=""urn:local:.:a00-bzmwvst"">JPF0512</a>)"
                     /note="/Transferred_From=JPF0512"
                     /note="/Transferred_Similarity=98.31%"
     polyA_signal    53..277
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:y-c2vthvs"">pDisplay-mSA-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:7t-c2wj41q"">Lenti__IVT_gBlock_msr-msd_ 
                     (Wt)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:7n-c8vy8oc"">pDisplay-mSA-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:87-c2wj420"">Lenti_IVT_gBlock_msr-msd_( 
                     TurboRFP_repair)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:1y-c61zrev"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:1x-c61zreu"">pDisplay-SS47 
                     (86)-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:8f-c2wj425"">Lenti_sgRNA_sfGFP-DO_no_Sp 
                     yCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:1x-c7wp0k7"">pDisplay-SS47 
                     (86)-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:19-c5kw4c2"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; bGH
                     poly(A) signal (<a 
                     href=""urn:local:.:6a-c8vxg0z"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)"
                     /note="/Transferred_From=pDisplay-SS47 (87)-EGFP-TM"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     complement(283..299)
                     /label=KJ_T0019_PolyA_Rev
     misc_feature    complement(402..413)
                     /label=polyArg
                     /note="polyarginine tag used for protein purification (may 
                     sometime have 6 R residues instead of 5)"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on protein similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     polyArg (<a href=""urn:local:.:37-c0ul19s"">SARS-CoV-2_S-2P
                     translation 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     polyArg (<a href=""urn:local:.:e-c79sdnm"">pDisplay-SS47 
                     (87)-EGFP-TM translation</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     polyArg (<a href=""urn:local:.:u-c79srj8"">S-2P_87_TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     polyArg (<a href=""urn:local:.:2g-c0ubf2q"">SARS-CoV-2_S-2P
                     translation 1</a>)"
                     /note="/Color=#FFFFB9"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     polyArg
                     (<a href=""urn:local:.:n-c79snqp"">S-2P_86_TM</a>)"
                     /note="/Transferred_From=S-2P_86_TM"
                     /note="/Transferred_Translation_Similarity=100.00%"
                     /note="/X-Alternative_Match=80.00%; 
                     Adjusted-Interval-0:3/3; <a 
                     href=""""urn:local:.:z1-bzc80bg"""">polyArg</a>"
                     /note="/X-Primary_Match=80.00%; Adjusted-Interval-0:0/0; <a
                     href=""""urn:local:.:z1-bzc80bg"""">polyArg</a>"
                     /note="/X-Transferred_From=polyArg"
                     /note="/X-Transferred_Translation_Similarity=80.00%"
                     /note="/kind=tag"
     primer_bind     538..557
                     /label=KJ_T0022_FRT_Fwd
     protein_bind    561..608
                     /label=FRT
                     /bound_moiety="FLP recombinase from the <i>Saccharomyces
                     cerevisiae</i> 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (<a 
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/21891781"" 
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/21891781"">Tura 
                     n and Bode, 2011</a>)."
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a 
                     href=""urn:local:.:v-c0u7dc2"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a 
                     href=""urn:local:.:973-bzmvybu"">pcDNA-FRT-TO-intron</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a 
                     href=""urn:local:.:3i-c0uqp26"">pcDNA5-intron-Spike-3XFLAG 
                     -TM-2A-EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a 
                     href=""urn:local:.:3f-c45uttj"">pcDNA5_TET_intron_HA_NLS_E 
                     co2_EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:2r-c0uhwai"">RFP-GFP 
                     reporter_pcDNA5-FRT-TO-intron_v1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a 
                     href=""urn:local:.:9ft-bzmwl6g"">pcDNA5-intron_HA_NLS_Eco1 
                     _FLAG</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:97c-bzmvyby"">pcDNA5 Reporter (RFP 
                     GFP) with hPGK promoter (replaced CMV)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a 
                     href=""urn:local:.:9fn-bzmwl6e"">pcDNA5-intron_HA_NLS_Eco1 
                     _EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a 
                     href=""urn:local:.:9fk-bzmwl6d"">pcDNA5-intron_HA_NLS_Eco1 
                     </a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; FRT
                     (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Transferred_From=pcDNA5_TET_intron_HA_NLS_Eco1_EGFP
                     "
                     /note="/Transferred_Similarity=100.00%"
     rep_origin      complement(575..608)
                     /direction=LEFT
                     /label=FRT site
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=89.47%; Adjusted-Interval-0:0/0; FRT 
                     site
                     (<a href=""urn:local:.:910-bzmvb6e"">pYTK082</a>)"
                     /note="/Transferred_From=pYTK082"
                     /note="/Transferred_Similarity=89.47%"
     protein_bind    complement(575..608)
                     /standard_name="FRT (minimal)"
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (minimal) (<a 
                     href=""urn:local:.:11-c4a760j"">Mu-793_R</a>)"
                     /note="/Primary_Match=82.35%; Adjusted-Interval-0:0/0; FRT
                     (minimal) (<a 
                     href=""urn:local:.:17-c4a760m"">Mu-795_R</a>)"
                     /note="/Transferred_From=Mu-795_R"
                     /note="/Transferred_Similarity=82.35%"
     misc_feature    575..608
                     /label=FRT
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:a3k-bzmwvuk"">MTK0_037</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:9l8-bzmwsfr"">MTK0_051</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:9l8-bzmwsfr"">MTK0_051</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:a44-bzmwvus"">MTK0_053</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:a44-bzmwvus"">MTK0_053</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:a2w-bzmwvub"">MTK0_028</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:a2w-bzmwvub"">MTK0_028</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:a2s-bzmwvu9"">MTK0_012</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     FRT
                     (<a href=""urn:local:.:a2s-bzmwvu9"">MTK0_012</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; FRT
                     (<a href=""urn:local:.:a3k-bzmwvuk"">MTK0_037</a>)"
                     /note="/Transferred_From=MTK0_037"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    complement(575..608)
                     /label=FRT
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (<a href=""urn:local:.:a3k-bzmwvuk"">MTK0_037</a>)"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (<a href=""urn:local:.:9l8-bzmwsfr"">MTK0_051</a>)"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (<a href=""urn:local:.:9l8-bzmwsfr"">MTK0_051</a>)"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (<a href=""urn:local:.:a44-bzmwvus"">MTK0_053</a>)"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (<a href=""urn:local:.:a44-bzmwvus"">MTK0_053</a>)"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (<a href=""urn:local:.:a2w-bzmwvub"">MTK0_028</a>)"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (<a href=""urn:local:.:a2w-bzmwvub"">MTK0_028</a>)"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (<a href=""urn:local:.:a2s-bzmwvu9"">MTK0_012</a>)"
                     /note="/Alternative_Match=82.35%; Adjusted-Interval-0:0/0; 
                     FRT
                     (<a href=""urn:local:.:a2s-bzmwvu9"">MTK0_012</a>)"
                     /note="/Primary_Match=82.35%; Adjusted-Interval-0:0/0; FRT 
                     (<a href=""urn:local:.:a3k-bzmwvuk"">MTK0_037</a>)"
                     /note="/Transferred_From=MTK0_037"
                     /note="/Transferred_Similarity=82.35%"
     CDS             610..1638
                     /codon_start=1
                     /gene="<i>aph(4)-Ia</i>"
                     /product="aminoglycoside phosphotransferase from <i>E.
                     coli</i>"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Adjusted_Transferred_Similarity=99.42%"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:6/0
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:v-c0u7dc2"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:6/0
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:973-bzmvybu"">pcDNA-FRT-TO-intron</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:6/0
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:3i-c0uqp26"">pcDNA5-intron-Spike-3XFLAG 
                     -TM-2A-EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:6/0
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:3f-c45uttj"">pcDNA5_TET_intron_HA_NLS_E 
                     co2_EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:6/0
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:2r-c0uhwai"">RFP-GFP 
                     reporter_pcDNA5-FRT-TO-intron_v1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:6/0
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:9ft-bzmwl6g"">pcDNA5-intron_HA_NLS_Eco1 
                     _FLAG</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:6/0
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:97c-bzmvyby"">pcDNA5 Reporter (RFP GFP) 
                     with hPGK promoter (replaced CMV)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:6/0
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:9fn-bzmwl6e"">pcDNA5-intron_HA_NLS_Eco1 
                     _EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:6/0
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:9fk-bzmwl6d"">pcDNA5-intron_HA_NLS_Eco1 
                     </a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:6/0 
                     (adjusted
                     to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Transferred_From=pcDNA5_TET_intron_HA_NLS_Eco1_EGFP
                     "
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=KKPELTATSVEKFLIEKFDSVSDLMQL
                     SEGEE 
                     SRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCI 
                     SRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRD 
                     FICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTD 
                     NGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAY 
                     MLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVE 
                     VLADSGNRRPSTRPRAKE*"
                     /translation="LAKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     misc_feature    675..680
                     /label=Esp3I, BsmBI
                     /note="Geneious type: restriction site"
                     /note="/Methylation=Mixed Methylation"
                     /note="/Recognition_pattern=CGTCTC(1/5)"
                     /note="/annotation_group=CGTCTC"
     primer_bind     complement(1648..1666)
                     /label=KJ_T0017_HygR_Rev
     primer_bind     1682..1698
                     /label=KJ_T0023_SV40_Fwd
     polyA_signal    complement(1768..1902)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=98.52%; Adjusted-Interval-0:0/0; 
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:3o-c0uw9uv"">pCEP4-BaLgp140-TM</a>)"
                     /note="/Primary_Match=98.52%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:e-c0uzs7w"">pDisplay-AP-CFP-TM</a>)"
                     /note="/Transferred_From=pDisplay-AP-CFP-TM"
                     /note="/Transferred_Similarity=98.52%"
     polyA_signal    1768..1889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9bv-bzmwcmo"">Lenti_FnCas9_sfGFP 
                     DO</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:95r-bzmvybf"">mNeptune2 CRISPEY msr 
                     msd</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:72-c0q55wp"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9 - KJ_T0505_IVT_gBlock_msr-msd_(Wt)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:95x-bzmvybg"">mNeptune2 
                     CRISPEY_81mer_sgRNA1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9c9-bzmwcmt"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9ea-bzmwl5z"">3XFLAG-NLS-RT</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:1l-c0q1vfs"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9em-bzmwl63"">HA-NLS-RT-EGFP</a>)"
                     /note="/Transferred_From=HA-NLS-RT-EGFP"
                     /note="/Transferred_Similarity=100.00%"
     polyA_signal    1768..1889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9e4-bzmwint"">Exported</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:7t-c2wj41q"">Lenti__IVT_gBlock_msr-msd_ 
                     (Wt)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:87-c2wj420"">Lenti_IVT_gBlock_msr-msd_( 
                     TurboRFP_repair)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:8f-c2wj425"">Lenti_sgRNA_sfGFP-DO_no_Sp 
                     yCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9ar-bzmvydt"">Exported</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:7z-c2wj41v"">Lenti_IVT_gBlock_msr-msd_( 
                     TurboRFP_repair)_HH_and_HDV</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9ia-bzmwl77"">Exported</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9az-bzmvydy"">Exported</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9an-bzmvydr"">Exported</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     SV40
                     poly(A) signal (<a 
                     href=""urn:local:.:9av-bzmvydw"">Exported</a>)"
                     /note="/Transferred_From=Exported"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    1771..1890
                     /label=SV40_PA_terminator
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40_PA_terminator (<a 
                     href=""urn:local:.:9ks-bzmwsfl"">MTK0_044</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0;
                     SV40_PA_terminator (<a 
                     href=""urn:local:.:9is-bzmwset"">MTK0_006</a>)"
                     /note="/Transferred_From=MTK0_006"
                     /note="/Transferred_Similarity=100.00%"
     terminator      complement(1773..1902)
                     /label=SV40-PA
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=98.46%; Adjusted-Interval-0:0/0;
                     SV40-PA (<a href=""urn:local:.:a34-bzmwvue"">MTK0_031</a>)"
                     /note="/Alternative_Match=98.46%; Adjusted-Interval-0:0/0;
                     SV40-PA (<a href=""urn:local:.:9yc-bzmwvrt"">ARB367</a>)"
                     /note="/Alternative_Match=98.46%; Adjusted-Interval-0:0/0;
                     SV40-PA (<a href=""urn:local:.:9yo-bzmwvs4"">ARB387</a>)"
                     /note="/Alternative_Match=98.46%; Adjusted-Interval-0:0/0;
                     SV40-PA (<a href=""urn:local:.:9yo-bzmwvs4"">ARB387</a>)"
                     /note="/Alternative_Match=98.46%; Adjusted-Interval-0:0/0;
                     SV40-PA (<a href=""urn:local:.:a1s-bzmwvtt"">JPF0536b</a>)"
                     /note="/Alternative_Match=98.46%; Adjusted-Interval-0:0/0;
                     SV40-PA (<a href=""urn:local:.:a44-bzmwvus"">MTK0_053</a>)"
                     /note="/Alternative_Match=98.46%; Adjusted-Interval-0:0/0;
                     SV40-PA (<a href=""urn:local:.:9y8-bzmwvrr"">ARB366</a>)"
                     /note="/Alternative_Match=98.46%; Adjusted-Interval-0:0/0;
                     SV40-PA (<a href=""urn:local:.:a1w-bzmwvtw"">JPF0536c</a>)"
                     /note="/Alternative_Match=98.46%; Adjusted-Interval-0:0/0;
                     SV40-PA (<a href=""urn:local:.:9y4-bzmwvro"">ARB365</a>)"
                     /note="/Primary_Match=98.46%; Adjusted-Interval-0:0/0; 
                     SV40-PA
                     (<a href=""urn:local:.:a3k-bzmwvuk"">MTK0_037</a>)"
                     /note="/Transferred_From=MTK0_037"
                     /note="/Transferred_Similarity=98.46%"
     primer_bind     1859..1878
                     /label=KJ_T0091_SV40_Rev
     promoter        complement(1934..1952)
                     /label=M13_reverse_primer
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     M13_reverse_primer (<a 
                     href=""urn:local:.:a38-bzmwvuf"">MTK0_033</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     M13_reverse_primer (<a 
                     href=""urn:local:.:a3g-bzmwvuj"">MTK0_036</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     M13_reverse_primer (<a 
                     href=""urn:local:.:a24-bzmwvu0"">MTK0_004</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     M13_reverse_primer (<a 
                     href=""urn:local:.:a3o-bzmwvul"">mtk0_045</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0;
                     M13_reverse_primer (<a 
                     href=""urn:local:.:a2w-bzmwvub"">MTK0_028</a>)"
                     /note="/Transferred_From=MTK0_028"
                     /note="/Transferred_Similarity=100.00%"
     promoter        complement(1942..2016)
                     /label=trc
                     /note="hybrid of the trp and lac promoters that is
                     stronger than the lac promoter"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     trc
                     (<a href=""urn:local:.:eh-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     trc
                     (<a href=""urn:local:.:d-c1aa132"">SrtA-PDGFR Tm</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     trc
                     (<a href=""urn:local:.:2t-c1pusju"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     trc
                     (<a href=""urn:local:.:18-c1ldurm"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     trc
                     (<a href=""urn:local:.:1o-c0u8hgs"">SARS-CoV-2_S-2P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     trc
                     (<a href=""urn:local:.:y-c0v8hwh"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     trc
                     (<a href=""urn:local:.:eg-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     trc
                     (<a href=""urn:local:.:ef-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     trc
                     (<a href=""urn:local:.:ee-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; trc
                     (<a href=""urn:local:.:x-c34iv9l"">SARS-CoV-2_S-2P</a>)"
                     /note="/Transferred_From=SARS-CoV-2_S-2P"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=82.67%; Adjusted-Interval-0:0/0; <a
                     href=""""urn:local:.:1oh-bzc80e2"""">trc</a>"
                     /note="/X-Transferred_From=trc"
                     /note="/X-Transferred_Similarity=82.67%"
                     /note="/regulatory_class=promoter"
     misc_feature    1949..1966
                     /label=TTCCTGTGTGAAATTGTT
                     /label=nonstandard type: motif
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/%_Identity=100"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     TTCCTGTGTGAAATTGTT (<a 
                     href=""urn:local:.:dis-bzmypex"">pDE43-MCK</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     TTCCTGTGTGAAATTGTT (<a 
                     href=""urn:local:.:dhw-bzmypec"">pDE43-MCK</a>)"
                     /note="/Mismatches=0"
                     /note="/Motif=TTCCTGTGTGAAATTGTT"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0;
                     TTCCTGTGTGAAATTGTT (<a 
                     href=""urn:local:.:df2-bzmypaq"">pDE43-MCK</a>)"
                     /note="/Transferred_From=pDE43-MCK"
                     /note="/Transferred_Similarity=100.00%"
     protein_bind    1958..1982
                     /label=lac operator
                     /bound_moiety="<i>lac</i> repressor encoded by
                     <i>lacI</i>"
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli</i>. This 
                     inhibition can be relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=96.00%; Adjusted-Interval-0:0/0; 
                     lac
                     operator (<a href=""urn:local:.:18-bzpsocj"">PX408 
                     Francisella tularensis subsp. novicida Cas9</a>)"
                     /note="/Alternative_Match=96.00%; Adjusted-Interval-0:0/0; 
                     lac
                     operator (<a href=""urn:local:.:9hf-bzmwl6v"">Twin Strep 
                     SUMO_HA NLS Ec67</a>)"
                     /note="/Alternative_Match=96.00%; Adjusted-Interval-0:0/0; 
                     lac
                     operator (<a href=""urn:local:.:9hq-bzmwl70"">Twin Strep 
                     SUMO_NLS Ec86</a>)"
                     /note="/Alternative_Match=96.00%; Adjusted-Interval-0:0/0; 
                     lac
                     operator (<a href=""urn:local:.:9h6-bzmwl6t"">Twin Strep 
                     SUMO_Ec86</a>)"
                     /note="/Alternative_Match=96.00%; Adjusted-Interval-0:0/0; 
                     lac
                     operator (<a 
                     href=""urn:local:.:52-bzpstax"">TS-SUMO-AcrIIA11-LPETGG-3x 
                     HA (1)</a>)"
                     /note="/Alternative_Match=96.00%; Adjusted-Interval-0:0/0; 
                     lac
                     operator (<a 
                     href=""urn:local:.:9bh-bzmwcmh"">6XHIS_MBP_TEV_3XFLAG_SpyC 
                     as9-pMJ806</a>)"
                     /note="/Alternative_Match=96.00%; Adjusted-Interval-0:0/0; 
                     lac
                     operator (<a href=""urn:local:.:98g-bzmvycj"">PX408 
                     Francisella tularensis subsp. novicida Cas9</a>)"
                     /note="/Alternative_Match=96.00%; Adjusted-Interval-0:0/0; 
                     lac
                     operator (<a 
                     href=""urn:local:.:40-bzpstad"">AcrIIA11-6xHis</a>)"
                     /note="/Alternative_Match=96.00%; Adjusted-Interval-0:0/0; 
                     lac
                     operator (<a 
                     href=""urn:local:.:9a0-bzmvyde"">TS-SUMO-AcrIIA12-LPETGG-3 
                     xHA</a>)"
                     /note="/Primary_Match=96.00%; Adjusted-Interval-0:0/0; lac
                     operator (<a href=""urn:local:.:9hk-bzmwl6y"">Twin Strep 
                     SUMO_HA NLS Ec86</a>)"
                     /note="/Transferred_From=Twin Strep SUMO_HA NLS Ec86"
                     /note="/Transferred_Similarity=96.00%"
     misc_binding    complement(1960..1982)
                     /label=LacO
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     LacO
                     (<a href=""urn:local:.:eh-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     LacO
                     (<a href=""urn:local:.:d-c14z9bb"">nCoV_1_AVITag</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     LacO
                     (<a href=""urn:local:.:2t-c1pusju"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     LacO
                     (<a href=""urn:local:.:18-c1ldurm"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     LacO
                     (<a href=""urn:local:.:1o-c0u8hgs"">SARS-CoV-2_S-2P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     LacO
                     (<a href=""urn:local:.:y-c0v8hwh"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     LacO
                     (<a href=""urn:local:.:eg-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     LacO
                     (<a href=""urn:local:.:ef-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     LacO
                     (<a href=""urn:local:.:ee-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     LacO (<a 
                     href=""urn:local:.:x-c34iv9l"">SARS-CoV-2_S-2P</a>)"
                     /note="/Transferred_From=SARS-CoV-2_S-2P"
                     /note="/Transferred_Similarity=100.00%"
     misc_binding    1960..1982
                     /label=LacO
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     LacO (<a 
                     href=""urn:local:.:9bq-bzmwcmn"">His-TS-SUMO-SpCas9-SBP2</ 
                     a>)"
                     /note="/Transferred_From=His-TS-SUMO-SpCas9-SBP2"
                     /note="/Transferred_Similarity=100.00%"
     protein_bind    1962..1978
                     /label=lac operator
                     /bound_moiety="<i>lac</i> repressor encoded by
                     <i>lacI</i>"
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli</i>. This 
                     inhibition can be relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a href=""urn:local:.:9es-bzmwl64"">Jenny's 
                     MBP-RT purification vector</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:9bv-bzmwcmo"">Lenti_FnCas9_sfGFP 
                     DO</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:72-c0q55wp"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9 - KJ_T0505_IVT_gBlock_msr-msd_(Wt)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:9c9-bzmwcmt"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:9ea-bzmwl5z"">3XFLAG-NLS-RT</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:1l-c0q1vfs"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a href=""urn:local:.:96x-bzmvybt"">Pax2</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; lac
                     operator (<a 
                     href=""urn:local:.:9em-bzmwl63"">HA-NLS-RT-EGFP</a>)"
                     /note="/Transferred_From=HA-NLS-RT-EGFP"
                     /note="/Transferred_Similarity=100.00%"
     protein_bind    complement(1962..1978)
                     /label=lac operator
                     /bound_moiety="<html><body><i>lac</i> repressor encoded 
                     by<i>lacI</i></body></html>"
                     /note="<html><body>The <i>lac</i> repressor binds to the 
                     <i>lac</i> operator to inhibit transcription in <i>E. 
                     coli</i>. This inhibition can be relieved by adding lactose
                     or isopropyl-beta-D-thiogalactopyranoside 
                     (IPTG).</body></html>"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a href=""urn:local:.:3a-c2pgpvs"">backup of 
                     Jenny's_3XFLAG-MBP-RT(Eco2/Ec67)-NLS-8XHIS</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:h-c0h0i2n"">Jenny's_3XFLAG-MBP-RT(Eco2/ 
                     Ec67)-NLS-8XHIS</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:1e-bzrqycc"">Jenny's_RT_(Eco1/Ec86)</a> 
                     )"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:12-bzrqybz"">Jenny's_MBP-RT_(Eco5/Ec107 
                     )</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:f-bzrqj20"">Jenny's_3XFLAG-MBP-RT(Eco1/ 
                     Ec86)-NLS-8XHIS</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:y-bzrqybu"">Jenny's_MBP-RT_(Eco2/Ec67)< 
                     /a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:6j-c2wj415"">Jenny's_3XFLAG-MBP-RT(Eco1 
                     /Ec86)-NLS-8XHIS_purification_vector</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:2g-c1vsgt7"">Jenny's_3XFLAG-MBP-RT(Eco2 
                     /Ec67)-NLS-8XHIS</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:g-c21rl6b"">Jenny's_3XFLAG-MBP-RT(Eco5/ 
                     Ec107)-NLS-8XHIS</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; lac
                     operator (<a 
                     href=""urn:local:.:m-c0h0i8d"">Jenny's_3XFLAG-MBP-RT(Eco5/ 
                     Ec107)-NLS-8XHIS</a>)"
                     /note="/Transferred_From=Jenny's_3XFLAG-MBP-RT(Eco5/Ec107)-
                     NLS-8
                     XHIS"
                     /note="/Transferred_Similarity=100.00%"
     protein_bind    1962..1978
                     /label=lac operator
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a href=""urn:local:.:d-c1aa132"">SrtA-PDGFR 
                     Tm</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:7t-c2wj41q"">Lenti__IVT_gBlock_msr-msd_ 
                     (Wt)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:87-c2wj420"">Lenti_IVT_gBlock_msr-msd_( 
                     TurboRFP_repair)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:26-bzrqycr"">pIF508_msr-msd_(TurboRFP_r 
                     epair)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:8f-c2wj425"">Lenti_sgRNA_sfGFP-DO_no_Sp 
                     yCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:7z-c2wj41v"">Lenti_IVT_gBlock_msr-msd_( 
                     TurboRFP_repair)_HH_and_HDV</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:9ia-bzmwl77"">Exported</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lac
                     operator (<a 
                     href=""urn:local:.:9b3-bzmvye1"">Exported</a>)"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/-5; lac
                     operator (<a 
                     href=""urn:local:.:51-c0q53l2"">KJ_T0507_IVT_gBlock_msr-ms 
                     d_(TurboRFP_repair)_HH_and_HDV</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; lac
                     operator (<a 
                     href=""urn:local:.:2c-bzrqycv"">pIF508_msr-msd_(TurboRFP_r 
                     epair)</a>)"
                     /note="/Transferred_From=pIF508_msr-msd_(TurboRFP_repair)"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    complement(1962..1978)
                     /label=Lac O1
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     Lac
                     O1 (<a href=""urn:local:.:af3-bzmyfmo"">pFF745</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     Lac
                     O1 (<a href=""urn:local:.:afe-bzmyfmt"">pFF745</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; Lac
                     O1
                     (<a href=""urn:local:.:aet-bzmyffh"">pFF745</a>)"
                     /note="/Transferred_From=pFF745"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    1962..1978
                     /label=lacO
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacO
                     (<a href=""urn:local:.:eh-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacO
                     (<a href=""urn:local:.:d-c1aa132"">SrtA-PDGFR Tm</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacO
                     (<a href=""urn:local:.:2t-c1pusju"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacO
                     (<a href=""urn:local:.:18-c1ldurm"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacO
                     (<a href=""urn:local:.:1o-c0u8hgs"">SARS-CoV-2_S-2P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacO
                     (<a href=""urn:local:.:y-c0v8hwh"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacO
                     (<a href=""urn:local:.:eg-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacO
                     (<a href=""urn:local:.:ef-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacO
                     (<a href=""urn:local:.:ee-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     lacO (<a 
                     href=""urn:local:.:x-c34iv9l"">SARS-CoV-2_S-2P</a>)"
                     /note="/Transferred_From=SARS-CoV-2_S-2P"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     <a href=""""urn:local:.:ph-bzc80ak"""">lacO</a>"
                     /note="/X-Transferred_From=lacO"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/kind=operator"
     misc_feature    1964..1978
                     /label=lacO
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on protein similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     lacO (<a href=""urn:local:.:37-c0ul19s"">SARS-CoV-2_S-2P 
                     translation 2</a>)"
                     /note="/Transferred_From=SARS-CoV-2_S-2P translation 2"
                     /note="/Transferred_Translation_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     <a href=""""urn:local:.:ph-bzc80ak"""">lacO</a>"
                     /note="/X-Transferred_From=lacO"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/kind=operator"
     misc_feature    1994..2005
                     /label=C
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on protein similarity"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     C
                     (<a href=""urn:local:.:eh-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     C
                     (<a href=""urn:local:.:d-c1aa132"">SrtA-PDGFR Tm</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     C
                     (<a href=""urn:local:.:2t-c1pusju"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     C
                     (<a href=""urn:local:.:18-c1ldurm"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     C
                     (<a href=""urn:local:.:1o-c0u8hgs"">SARS-CoV-2_S-2P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     C
                     (<a href=""urn:local:.:y-c0v8hwh"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     C
                     (<a href=""urn:local:.:eg-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     C
                     (<a href=""urn:local:.:ef-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     C
                     (<a href=""urn:local:.:ee-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Color=#FFFFB9"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; C 
                     (<a href=""urn:local:.:x-c34iv9l"">SARS-CoV-2_S-2P</a>)"
                     /note="/Transferred_From=SARS-CoV-2_S-2P"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     <a href=""""urn:local:.:te-bzc80b3"""">C</a>"
                     /note="/X-Transferred_From=C"
                     /note="/X-Transferred_Translation_Similarity=100.00%"
                     /note="/kind=tag"
     regulatory      2031..2052
                     /regulatory_class="other"
                     /label=CAP BS
                     /note="catabolite activator protein binding site"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     BS (<a href=""urn:local:.:eh-c30wztu"">SARS-CoV-2_S-2P_Cell
                     Surface 4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     BS (<a href=""urn:local:.:d-c1aa132"">SrtA-PDGFR Tm</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     BS (<a href=""urn:local:.:2t-c1pusju"">SARS-CoV-2_S-2P_Cell
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     BS (<a href=""urn:local:.:18-c1ldurm"">SARS-CoV-2_S-2P_Cell
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     BS (<a 
                     href=""urn:local:.:1o-c0u8hgs"">SARS-CoV-2_S-2P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     BS (<a href=""urn:local:.:y-c0v8hwh"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     BS (<a href=""urn:local:.:eg-c30wztu"">SARS-CoV-2_S-2P_Cell
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     BS (<a href=""urn:local:.:ef-c30wztu"">SARS-CoV-2_S-2P_Cell
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     BS (<a href=""urn:local:.:ee-c30wztu"">SARS-CoV-2_S-2P_Cell
                     Surface 1</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; CAP
                     BS
                     (<a href=""urn:local:.:x-c34iv9l"">SARS-CoV-2_S-2P</a>)"
                     /note="/Transferred_From=SARS-CoV-2_S-2P"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     <a href=""""urn:local:.:1v4-bzc80f3"""">CAP BS</a>"
                     /note="/X-Transferred_From=CAP BS"
                     /note="/X-Transferred_Similarity=100.00%"
     regulatory      2031..2052
                     /regulatory_class="other"
                     /standard_name="CAP BS"
                     /label=CAP BS
                     /note="catabolite activator protein binding site"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=95.45%; Adjusted-Interval-0:0/0; 
                     CAP
                     BS (<a href=""urn:local:.:11-c4pu1xm"">pMAZ-SK_795</a>)"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:2/-2; CAP
                     BS (<a href=""urn:local:.:37-c0ul19s"">SARS-CoV-2_S-2P 
                     translation 2</a>)"
                     /note="/Primary_Match=95.45%; Adjusted-Interval-0:0/0; CAP 
                     BS
                     (<a href=""urn:local:.:y-c4pu1xk"">pMAZ-SK_794</a>)"
                     /note="/Transferred_From=pMAZ-SK_794"
                     /note="/Transferred_Similarity=95.45%"
     protein_bind    2031..2052
                     /label=CAP binding site
                     /bound_moiety="<i>E. coli</i> catabolite activator
                     protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:9bv-bzmwcmo"">Lenti_FnCas9_sfGFP 
                     DO</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:72-c0q55wp"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9 - KJ_T0505_IVT_gBlock_msr-msd_(Wt)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:9c9-bzmwcmt"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:9ea-bzmwl5z"">3XFLAG-NLS-RT</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:1l-c0q1vfs"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:96x-bzmvybt"">Pax2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:v-c0u7dc2"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; CAP
                     binding site (<a 
                     href=""urn:local:.:9em-bzmwl63"">HA-NLS-RT-EGFP</a>)"
                     /note="/Transferred_From=HA-NLS-RT-EGFP"
                     /note="/Transferred_Similarity=100.00%"
     protein_bind    2031..2052
                     /label=CAP binding site
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a href=""urn:local:.:d-c1aa132"">SrtA-PDGFR 
                     Tm</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:7t-c2wj41q"">Lenti__IVT_gBlock_msr-msd_ 
                     (Wt)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:87-c2wj420"">Lenti_IVT_gBlock_msr-msd_( 
                     TurboRFP_repair)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:26-bzrqycr"">pIF508_msr-msd_(TurboRFP_r 
                     epair)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:8f-c2wj425"">Lenti_sgRNA_sfGFP-DO_no_Sp 
                     yCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:7z-c2wj41v"">Lenti_IVT_gBlock_msr-msd_( 
                     TurboRFP_repair)_HH_and_HDV</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:9ia-bzmwl77"">Exported</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CAP
                     binding site (<a 
                     href=""urn:local:.:9b3-bzmvye1"">Exported</a>)"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:2/-2; CAP
                     binding site (<a 
                     href=""urn:local:.:37-c0ul19s"">SARS-CoV-2_S-2P translation
                     2</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; CAP
                     binding site (<a 
                     href=""urn:local:.:2c-bzrqycv"">pIF508_msr-msd_(TurboRFP_r 
                     epair)</a>)"
                     /note="/Transferred_From=pIF508_msr-msd_(TurboRFP_repair)"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    2159..2187
                     /label=RSF 1030 ori
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=96.55%; Adjusted-Interval-0:0/0; 
                     RSF
                     1030 ori (<a 
                     href=""urn:local:.:dqg-bzmyr4p"">pR6K_FRT_Kan</a>)"
                     /note="/Primary_Match=96.55%; Adjusted-Interval-0:0/0; RSF 
                     1030
                     ori (<a href=""urn:local:.:dtl-bzmyr9x"">pR6K_FRT_Kan</a>)"
                     /note="/Transferred_From=pR6K_FRT_Kan"
                     /note="/Transferred_Similarity=96.55%"
     unsure          2266..2305
                     /label=Spacer
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     Spacer (<a 
                     href=""urn:local:.:a4-c2wj42s"">pKJ0009:_ES.entry_-_Type_4 
                     _Spacer_(T0432_T0433)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     Spacer (<a 
                     href=""urn:local:.:9s-c2wj42n"">pKJ0007:_ES.entry_-_Type_2 
                     _Spacer_(T0428_T0429)</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Spacer
                     (<a 
                     href=""urn:local:.:9y-c2wj42p"">pKJ0008:_ES.entry_-_Type_3 
                     _Spacer_(T0430_T0431)</a>)"
                     /note="/Transferred_From=pKJ0008:_ES.entry_-_Type_3_Spacer_
                     (T043
                     0_T0431)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=moham"
     primer_bind     complement(2280..2296)
                     /label=KJ_T0040_ColE1_Fwd
     misc_feature    2325..2944
                     /label=pBR322
                     /label=nonstandard type: ori
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=99.84%; Adjusted-Interval-0:0/0; 
                     pBR322
                     (<a href=""urn:local:.:x-c8vvcds"">pKAR2</a>)"
                     /note="/Transferred_From=pKAR2"
                     /note="/Transferred_Similarity=99.84%"
                     /note="/created_by=User"
     rep_origin      2340..2928
                     /label=ori
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; ori
                     (<a href=""urn:local:.:w-bzpsocc"">EZeo vector_Simon</a>)"
                     /note="/Transferred_From=EZeo vector_Simon"
                     /note="/Transferred_Similarity=100.00%"
     misc_RNA        complement(2556..2585)
                     /label=crRNA-ColA Ori
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=83.33%; Adjusted-Interval-0:0/0;
                     crRNA-ColA Ori (<a 
                     href=""urn:local:.:i-c21rl6b"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Alternative_Match=83.33%; Adjusted-Interval-0:0/0;
                     crRNA-ColA Ori (<a 
                     href=""urn:local:.:2u-c1vshzl"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Alternative_Match=83.33%; Adjusted-Interval-0:0/0;
                     crRNA-ColA Ori (<a 
                     href=""urn:local:.:y-c4pu1xk"">pMAZ-SK_794</a>)"
                     /note="/Alternative_Match=83.33%; Adjusted-Interval-0:0/0;
                     crRNA-ColA Ori (<a 
                     href=""urn:local:.:11-c4pu1xm"">pMAZ-SK_795</a>)"
                     /note="/Alternative_Match=83.33%; Adjusted-Interval-0:0/0;
                     crRNA-ColA Ori (<a 
                     href=""urn:local:.:85-c5ca8r3"">pMAZ-SK</a>)"
                     /note="/Alternative_Match=83.33%; Adjusted-Interval-0:0/0;
                     crRNA-ColA Ori (<a 
                     href=""urn:local:.:2f-c0hk8ws"">pMAZ-SK</a>)"
                     /note="/Primary_Match=83.33%; Adjusted-Interval-0:0/0;
                     crRNA-ColA Ori (<a 
                     href=""urn:local:.:1zc-bzlzoz3"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Transferred_From=pMAZ-SK_sfGFP DO"
                     /note="/Transferred_Similarity=83.33%"
                     /note="/created_by=moham"
                     /note="/modified_by=moham"
     misc_feature    complement(2945..3003)
                     /label=pMB1 region
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     pMB1
                     region (<a href=""urn:local:.:x-c8vvcds"">pKAR2</a>)"
                     /note="/Transferred_From=pKAR2"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_difference 2950..2967
                     /label=KanF_seq
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/%_Identity=100"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     KanF_seq (<a 
                     href=""urn:local:.:dpm-bzmyqla"">kanRnullwithSpec</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     KanF_seq (<a 
                     href=""urn:local:.:d2l-bzmyo2r"">kanRnullwithSpec</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     KanF_seq (<a 
                     href=""urn:local:.:ckv-bzmynor"">kanRnullwithSpec</a>)"
                     /note="/Motif=CTGACGCTCAG"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     KanF_seq
                     (<a href=""urn:local:.:dp0-bzmyqkg"">kanRnullwithSpec</a>)"
                     /note="/Transferred_From=kanRnullwithSpec"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/modified_by=User"
     primer_bind     complement(2979..3000)
                     /label=KJ_T0018_Ori_Rev
     misc_feature    2983..3003
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0;  
                     (<a href=""urn:local:.:x-c8vvcds"">pKAR2</a>)"
                     /note="/Transferred_From=pKAR2"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     complement(2984..3014)
                     /label=KJ_T0470_Ec86_Beta_Backbone_rev
     primer_bind     2995..3033
                     /label=KJ_T0471_AmpR_Insert_fwd
     misc_feature    3000..3021
                     /label=Geneious type: structural annotation
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Geneious type: structural annotation"
                     /note="/Primary_Match=81.82%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:.:aq-c2wk6f6"">TU3ER</a>)"
                     /note="/Transferred_From=TU3ER"
                     /note="/Transferred_Similarity=81.82%"
                     /note="/created_by=User"
     terminator      complement(3005..3101)
                     /label=AmpR Terminator
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Terminator (<a 
                     href=""urn:local:.:a34-bzmwvue"">MTK0_031</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Terminator (<a 
                     href=""urn:local:.:9ik-bzmwsep"">MTK0_001</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Terminator (<a 
                     href=""urn:local:.:9kc-bzmwsfg"">MTK0_025</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Terminator (<a 
                     href=""urn:local:.:9x4-bzmwvr9"">ARB343</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Terminator (<a 
                     href=""urn:local:.:9xk-bzmwvrf"">ARB347</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Terminator (<a 
                     href=""urn:local:.:9jw-bzmwsfa"">MTK0_021</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Terminator (<a href=""urn:local:.:aq-c2wk6f6"">TU3ER</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Terminator (<a 
                     href=""urn:local:.:92g-bzmvb6x"">pYTK095</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Terminator (<a 
                     href=""urn:local:.:9x8-bzmwvrb"">ARB344</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR
                     Terminator (<a 
                     href=""urn:local:.:9ww-bzmwvr7"">ARB341</a>)"
                     /note="/Transferred_From=ARB341"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    complement(3012..3060)
                     /label=terminator
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     terminator (<a 
                     href=""urn:local:.:6g-c4ce7j9"">P114-R5-GfOMT1_2.4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     terminator (<a 
                     href=""urn:local:.:d-c3b6zke"">P114-R5-GfOMT1_1.6</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     terminator (<a 
                     href=""urn:local:.:h-c3b6zrl"">P114-R5-GfOMT1_2.4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     terminator (<a 
                     href=""urn:local:.:o-c3b76y3"">P114-R5-GfOMT1_2.4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     terminator (<a 
                     href=""urn:local:.:n-c3b76y3"">P114-R5-GfOMT1_1.6</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0;
                     terminator (<a 
                     href=""urn:local:.:6f-c4ce7j9"">P114-R5-GfOMT1_1.6</a>)"
                     /note="/Transferred_From=P114-R5-GfOMT1_1.6"
                     /note="/Transferred_Similarity=100.00%"
     CDS             complement(3099..3959)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (<a 
                     href=""urn:local:.:2c-bzrqycv"">pIF508_msr-msd_(TurboRFP_r 
                     epair)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (<a 
                     href=""urn:local:.:y-c2vthvs"">pDisplay-mSA-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (<a href=""urn:local:.:d-c1aa132"">SrtA-PDGFR Tm</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (<a 
                     href=""urn:local:.:7n-c8vy8oc"">pDisplay-mSA-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (<a href=""urn:local:.:1y-c61zrev"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (<a 
                     href=""urn:local:.:26-bzrqycr"">pIF508_msr-msd_(TurboRFP_r 
                     epair)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (<a href=""urn:local:.:1x-c61zreu"">pDisplay-SS47 
                     (86)-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (<a href=""urn:local:.:1x-c7wp0k7"">pDisplay-SS47 
                     (86)-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     (<a href=""urn:local:.:19-c5kw4c2"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR (<a href=""urn:local:.:6a-c8vxg0z"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)"
                     /note="/Transferred_From=pDisplay-SS47 (87)-EGFP-TM"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=MSIQHFRVALIPFFAAFCLPVFAHPET
                     LVKVK 
                     DAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLG 
                     RRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTA 
                     FLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLI 
                     DWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGS 
                     QATMDERNRQIAEIGASLIKHW"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(3239..3244)
                     /label=BsaI
                     /note="Geneious type: restriction site"
                     /note="/Methylation=Dcm:variable, CpG:variable, EcoBI:cut"
                     /note="/Recognition_pattern=GGTCTC(1/5)"
                     /note="/annotation_group=GGTCTC"
     promoter        complement(3960..4090)
                     /label=AmpR Promoter
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Promoter (<a 
                     href=""urn:local:.:a34-bzmwvue"">MTK0_031</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Promoter (<a 
                     href=""urn:local:.:9ik-bzmwsep"">MTK0_001</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Promoter (<a 
                     href=""urn:local:.:9kc-bzmwsfg"">MTK0_025</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Promoter (<a href=""urn:local:.:9x4-bzmwvr9"">ARB343</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Promoter (<a href=""urn:local:.:9xk-bzmwvrf"">ARB347</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Promoter (<a 
                     href=""urn:local:.:9jw-bzmwsfa"">MTK0_021</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Promoter (<a href=""urn:local:.:aq-c2wk6f6"">TU3ER</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Promoter (<a href=""urn:local:.:92g-bzmvb6x"">pYTK095</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     Promoter (<a href=""urn:local:.:9x8-bzmwvrb"">ARB344</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR
                     Promoter (<a href=""urn:local:.:9ww-bzmwvr7"">ARB341</a>)"
                     /note="/Transferred_From=ARB341"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    complement(4001..4029)
                     /label=AmpR promoter
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (<a 
                     href=""urn:local:.:6g-c4ce7j9"">P114-R5-GfOMT1_2.4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (<a 
                     href=""urn:local:.:d-c3b6zke"">P114-R5-GfOMT1_1.6</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (<a 
                     href=""urn:local:.:h-c3b6zrl"">P114-R5-GfOMT1_2.4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (<a 
                     href=""urn:local:.:o-c3b76y3"">P114-R5-GfOMT1_2.4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AmpR
                     promoter (<a 
                     href=""urn:local:.:n-c3b76y3"">P114-R5-GfOMT1_1.6</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR
                     promoter (<a 
                     href=""urn:local:.:6f-c4ce7j9"">P114-R5-GfOMT1_1.6</a>)"
                     /note="/Transferred_From=P114-R5-GfOMT1_1.6"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    4007..4023
                     /label=lacO
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     lacO (<a href=""urn:local:.:d-c1aa132"">SrtA-PDGFR Tm</a>)"
                     /note="/Transferred_From=SrtA-PDGFR Tm"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=70.59%; Adjusted-Interval-0:0/0; <a
                     href=""""urn:local:.:ph-bzc80ak"""">lacO</a>"
                     /note="/X-Transferred_From=lacO"
                     /note="/X-Transferred_Similarity=70.59%"
                     /note="/kind=operator"
     polyA_site      complement(4008..4037)
                     /label=Spec_F
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Spec_F
                     (<a href=""urn:local:.:d1q-bzmyo2k"">kanRnullwithSpec</a>)"
                     /note="/Transferred_From=kanRnullwithSpec"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     4294..4308
                     /label=KJ_T0021_CMV_Fwd
     primer_bind     complement(4313..4329)
                     /label=KJ_T0156_CMV_Rev
     enhancer        4330..4709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     enhancer (<a 
                     href=""urn:local:.:9bv-bzmwcmo"">Lenti_FnCas9_sfGFP 
                     DO</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     enhancer (<a 
                     href=""urn:local:.:72-c0q55wp"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9 - KJ_T0505_IVT_gBlock_msr-msd_(Wt)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     enhancer (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     enhancer (<a 
                     href=""urn:local:.:9c9-bzmwcmt"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     enhancer (<a 
                     href=""urn:local:.:9ea-bzmwl5z"">3XFLAG-NLS-RT</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     enhancer (<a 
                     href=""urn:local:.:1l-c0q1vfs"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     enhancer (<a href=""urn:local:.:96x-bzmvybt"">Pax2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     enhancer (<a 
                     href=""urn:local:.:9e7-bzmwl5y"">3XFLAG-NLS-RT-EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     enhancer (<a 
                     href=""urn:local:.:973-bzmvybu"">pcDNA-FRT-TO-intron</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; CMV
                     enhancer (<a 
                     href=""urn:local:.:9em-bzmwl63"">HA-NLS-RT-EGFP</a>)"
                     /note="/Transferred_From=HA-NLS-RT-EGFP"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     4462..4481
                     /label=KJ_T0234_pAAV_CMV_Fwd
     promoter        4710..4913
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     promoter (<a href=""urn:local:.:95r-bzmvybf"">mNeptune2 
                     CRISPEY msr msd</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     promoter (<a 
                     href=""urn:local:.:9av-bzmvydw"">Exported</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     promoter (<a href=""urn:local:.:95x-bzmvybg"">mNeptune2 
                     CRISPEY_81mer_sgRNA1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     promoter (<a href=""urn:local:.:6a-c8vxg0z"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     promoter (<a 
                     href=""urn:local:.:9ea-bzmwl5z"">3XFLAG-NLS-RT</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     promoter (<a href=""urn:local:.:96x-bzmvybt"">Pax2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     promoter (<a href=""urn:local:.:18-bzpsocj"">PX408 
                     Francisella tularensis subsp. novicida Cas9</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     promoter (<a 
                     href=""urn:local:.:9e7-bzmwl5y"">3XFLAG-NLS-RT-EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     CMV
                     promoter (<a 
                     href=""urn:local:.:973-bzmvybu"">pcDNA-FRT-TO-intron</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; CMV
                     promoter (<a 
                     href=""urn:local:.:9em-bzmwl63"">HA-NLS-RT-EGFP</a>)"
                     /note="/Transferred_From=HA-NLS-RT-EGFP"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     4888..4907
                     /label=KJ_T0196_CMV_Fwd
     protein_bind    join(4915..4933,4936..4954)
                     /label=TetO
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:1z8-bzlzoz0"">pMA7CR_2.0</a>)"
                     /note="/Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:1z8-bzlzoz0"">pMA7CR_2.0</a>)"
                     /note="/Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:1zc-bzlzoz3"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:1zc-bzlzoz3"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:1zc-bzlzoz3"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:1zc-bzlzoz3"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:i-c21rl6b"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:i-c21rl6b"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:i-c21rl6b"">pMAZ-SK_sfGFP DO</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0, 
                     Adjusted-Interval-1:0/0; TetO (<a 
                     href=""urn:local:.:ai-c2wk6ez"">pSK.102</a>)"
                     /note="/Transferred_From=pSK.102"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     protein_bind    4915..4933
                     /gene="<i>tetO</i>"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O<sub>2</sub> for the
                     <i>tetR</i> and <i>tetA</i> genes"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Transferred_From=Lenti_TET_HA_NLS_EcoI_EGFP"
                     /note="/Transferred_Similarity=100.00%"
     protein_bind    4936..4954
                     /gene="<i>tetO</i>"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O<sub>2</sub> for the
                     <i>tetR</i> and <i>tetA</i> genes"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     tet
                     operator (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; tet
                     operator (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)"
                     /note="/Transferred_From=Lenti_TET_HA_NLS_EcoI_EGFP"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     4957..4974
                     /label=KJ_T0158_CMV_Fwd
     primer_bind     4972..4989
                     /label=KJ_T0010_AcGFP1 Seq_Fwd
     misc_feature    complement(4994..4999)
                     /label=Esp3I, BsmBI
                     /note="Geneious type: restriction site"
                     /note="/Methylation=Mixed Methylation"
                     /note="/Recognition_pattern=CGTCTC(1/5)"
                     /note="/annotation_group=CGTCTC"
     primer_bind     5033..5047
                     /label=KJ_T0024_TurboRFP_Fwd
     intron          5107..5239
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     chimeric intron (<a 
                     href=""urn:local:.:9b3-bzmvye1"">Exported</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     chimeric
                     intron (<a href=""urn:local:.:9ia-bzmwl77"">Exported</a>)"
                     /note="/Transferred_From=Exported"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    complement(5138..5143)
                     /label=BsaI
                     /note="Geneious type: restriction site"
                     /note="/Methylation=Dcm:variable, CpG:variable, EcoBI:cut"
                     /note="/Recognition_pattern=GGTCTC(1/5)"
                     /note="/annotation_group=GGTCTC"
     primer_bind     5274..5302
                     
     promoter        5284..5302
                     /label=T7 Promoter
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     T7
                     Promoter (<a 
                     href=""urn:local:.:9m-c2wj42l"">pKJ0006:_ES.entry_-_T7_Pro 
                     moter_RBS1_(T0434_T0435)</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     T7
                     Promoter (<a href=""urn:local:.:8n-c2wj429"">pKJ0001</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     T7
                     Promoter (<a 
                     href=""urn:local:.:6y-c2wj41d"">KJ_T0417_msr_Eco1_</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     T7
                     promoter (<a 
                     href=""urn:local:.:9em-bzmwl63"">HA-NLS-RT-EGFP</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     T7
                     promoter (<a 
                     href=""urn:local:.:1e-c4vgcq6"">KJ_T0630_IVT_gBlock_msr-ms 
                     d RFP repair </a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     T7
                     promoter (<a href=""urn:local:.:9hk-bzmwl6y"">Twin Strep 
                     SUMO_HA NLS Ec86</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     T7
                     promoter (<a 
                     href=""urn:local:.:97w-bzmvyc8"">pMBP-AsCas12a</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     T7
                     promoter (<a 
                     href=""urn:local:.:52-c0q53l2"">KJ_T0518_IVT_gBlock_msr-ms 
                     d_(Wt)_mutant_1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     T7
                     promoter (<a href=""urn:local:.:6a-c8vxg0z"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; T7
                     Promoter (<a 
                     href=""urn:local:.:6r-c2wj41b"">KJ_T0417_msr_Eco1__-_mScar 
                     let-DO_(T0417/T0418)_-_KJ_T0418_msd_Eco1_</a>)"
                     /note="/Transferred_From=T7 Promoter"
                     /note="/Transferred_From=KJ_T0417_msr_Eco1__-_mScarlet-DO_(
                     T0417 /T0418)_-_KJ_T0418_msd_Eco1_"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=moham"
     primer_bind     complement(5329..5357)
                     
     primer_bind     complement(5330..5347)
                     /label=KJ_T0045_pcDNA5_NotI_Rev
     primer_bind     5338..5368
                     
     CDS             5348..5410
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     misc_feature    5352..5409
                     /label=GCGGGGAGGGCCTTCGTGCGTCGCCGCGCCGCCGTCCCCTTCTCCCT
                     /label=GCGGGGAGGGCCTTCGTGCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTC 
                     CAGCCTCGGGGCTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGG 
                     GGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGCGCCTCTGCTAACCATGTTCATGC 
                     CTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTTGTTGTGCTGTCTCATCAT 
                     TTTGGCAAAGAATTGCGGCCGTCTCAGGCCGAGTTCGGTACCGCCAC
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=GCGGGGAGGGCCTTCGTGCGTCGCCGCGCCGCCGTC
                     CCCTT 
                     CTCCCTCTCCAGCCTCGGGGCTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGG 
                     GCAGGGCGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGCGCCTCTGCTAACCA 
                     TGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTTGTTGTGCTG 
                     TCTCATCATTTTGGCAAAGAATTGCGGCCGTCTCAGGCCGAGTTCGGTACCGCCAC"
     misc_feature    5407..5410
                     /label=Part 1a 5' Overhang
                     /label=nonstandard type: overhang
                     /note="/created_by=moham"
                     /note="/modified_by=moham"
     CDS             5411..9611
                     /codon_start=1
                     /label=SARS-CoV-2 Spike Protein
                     /note="/Original_Translation=MFVFLVLLPLVSSQCVNLTTRTQLPPAYTN
                     SFTRG 
                     VYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFA 
                     STEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKS 
                     WMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTP 
                     INLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYV 
                     GYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTES 
                     IVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVS 
                     PTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNL 
                     DSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQP 
                     TNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNK 
                     KFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDV 
                     NCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICAS 
                     YQTQTNSPGSASSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSM 
                     TKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIY 
                     KTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAAR 
                     DLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYR 
                     FNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLV 
                     KQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRAS 
                     ANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAP 
                     AICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTV 
                     YDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNE 
                     SLIDLQELGKYEQ"
                     /note="/modified_by=moham"
                     /translation="MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRS
                     SVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGW
                     IFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSA
                     NNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSA
                     LEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNE
                     NGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGE
                     VFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADS
                     FVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRK
                     SNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFEL
                     LHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAV
                     RDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRV
                     YSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPGSASSVASQSIIA
                     YTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLL
                     LQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKP
                     SKRSPIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIA
                     QYTSALLAGTITSGWTFGAGPALQIPFPMQMAYRFNGIGVTQNVLYENQKLIANQFNSA
                     IGKIQDSLSSTPSALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDPPEA
                     EVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYH
                     LMSFPQRWQVESET*FHASF*TFCKSYLIMCAAIHI*FMNVFLTSRQLKKRQVRILATR
                     ERGEILDA*RRRAVHWCRPYSGGTGW*CQRS*VFRAWRG*R*RN*W*TDAEVHLYYW*T
                     AGSLADSGNDADLWCSVLCSLSGPYEAA*LLQVRHAGRLCAGTHDFL*G*RHVQNACGS
                     EI*RRYPGKPH*AERH*L*RGRQYPGP*AGIQF*QPQCLHHRR*TKKWH*SEF*NSPQR
                     GGWQRAAG*SLPAKHSNR*WSCSAARQSLSEHAKRSV*RSERETRSYGSAGVRNRSGHH
                     AWYG*TVQMTRHQIKRKAQSKDWAFRFICCLSVNALY*SHTGSPSGGPFCVYTPATA"
     gene            5489..6325
                     /label=NTD
                     /note="/created_by=moham"
     misc_feature    5817..5820
                     /label=Part 1a 3' Overhang
                     /label=nonstandard type: overhang
                     /note="/created_by=moham"
                     /note="/modified_by=moham"
     misc_feature    5817..5820
                     /label=Part 1b 5' Overhang
                     /label=nonstandard type: overhang
                     /note="/created_by=moham"
                     /note="/modified_by=moham"
     misc_feature    5920..5925
                     /label=Esp3I, BsmBI
                     /note="Geneious type: restriction site"
                     /note="/Methylation=Mixed Methylation"
                     /note="/Recognition_pattern=CGTCTC(1/5)"
                     /note="/annotation_group=CGTCTC"
     primer_bind     6140..6162
                     
     misc_feature    6208..6211
                     /label=Part 1b 3' Overhang
                     /label=nonstandard type: overhang
                     /note="/created_by=moham"
                     /note="/modified_by=moham"
     misc_feature    6208..6211
                     /label=Part 2 5' Overhang
                     /label=nonstandard type: overhang
                     /note="/created_by=moham"
                     /note="/modified_by=moham"
     primer_bind     complement(6279..6297)
                     
     gene            6326..6973
                     /label=RBD
                     /note="/created_by=moham"
     misc_feature    6773..6775
                     /label=L455
                     /label=nonstandard type: Polymorphism
                     /note="/created_by=moham"
     misc_feature    6902..6904
                     /label=Q498
                     /label=nonstandard type: Polymorphism
                     /note="/created_by=moham"
     misc_feature    6911..6913
                     /label=N501
                     /label=nonstandard type: Polymorphism
                     /note="/created_by=moham"
     misc_feature    6914..6916
                     /label=G502
                     /label=nonstandard type: Polymorphism
                     /note="/created_by=moham"
     gene            6974..8131
                     /label=FP
                     /note="/created_by=moham"
     misc_feature    6974..6977
                     /label=Part 2 3' Overhang
                     /label=nonstandard type: overhang
                     /note="/created_by=moham"
     primer_bind     complement(7036..7055)
                     
     misc_feature    7251
                     /label=A
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=A"
     misc_feature    7771..7774
                     /label=overhang
                     /note="/created_by=moham"
     misc_feature    7859..7860
                     /label=TT
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=TT"
     misc_feature    8084
                     /label=G
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=G"
     misc_feature    8105
                     /label=G
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=G"
     gene            8133..9611
                     /label=HR, CH, and CTD
                     /note="/created_by=moham"
     misc_feature    8234
                     /label=G
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=G"
     misc_feature    8570..8573
                     /label=Part 4 3'
                     /label=nonstandard type: overhang
                     /note="/created_by=moham"
     misc_feature    complement(8578..8584)
                     /label=AarI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CACCTGC(4/8)"
                     /note="/annotation_group=CACCTGC"
     promoter        8585..8714
                     /label=BBa_J72163 GlpT Promoter
     misc_feature    8715..8750
                     /label=sfGFP Ribosome Binding Site
     RBS             8733..8744
                     /label=RBS
                     /note="strong bacterial ribosome binding site (<a 
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/10659856"" 
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/10659856"">Elow 
                     itz and Leibler, 2000</a>)"
     CDS             8751..9464
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused
                     to poorly folded proteins (<a 
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/16369541"" 
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/16369541"">Pede 
                     lacq et al., 2006</a>)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      9476..9547
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the <i>E. coli
                     rrnB</i> gene"
     terminator      9563..9590
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    9597..9603
                     /label=AarI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CACCTGC(4/8)"
                     /note="/annotation_group=CACCTGC"
     misc_feature    9608..9611
                     /label=3' overhang
                     /label=nonstandard type: overhang
                     /note="/created_by=moham"
     misc_feature    9621..9701
                     /label=Foldon
     CDS             9708..9773
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             9780..9803
                     /label=Strep Tag II
                     /note="/created_by=moham"
     CDS             9810..9833
                     /label=PreScission site (3C)
                     /note="/created_by=moham"
                     /note="/modified_by=moham"
     gene            9876..10023
                     /label=PDGFR-B TM domain
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     PDGFR-B TM domain (<a 
                     href=""urn:local:.:2t-c1pusju"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     PDGFR-B TM domain (<a 
                     href=""urn:local:.:18-c1ldurm"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     PDGFR-B TM domain (<a 
                     href=""urn:local:.:y-c0v8hwh"">SARS-CoV-2_S-2P_Cell Surface
                     1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     PDGFR-B TM domain (<a 
                     href=""urn:local:.:eg-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     PDGFR-B TM domain (<a 
                     href=""urn:local:.:ef-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     PDGFR-B TM domain (<a 
                     href=""urn:local:.:ee-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     PDGFR-B TM domain (<a 
                     href=""urn:local:.:4y-c1pwmyb"">SARS-CoV-2_S-2P_Cell 
                     Surface 4</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     PDGFR-B TM domain (<a 
                     href=""urn:local:.:h-c9zxg2l"">Spike-hinge-TM extract</a>)"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     PDGFR-B TM domain (<a 
                     href=""urn:local:.:n-c79snqp"">S-2P_86_TM</a>)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     PDGFR-B
                     TM domain (<a 
                     href=""urn:local:.:eh-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 4</a>)"
                     /note="/Transferred_From=SARS-CoV-2_S-2P_Cell Surface 4"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=moham"
     misc_feature    9924
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
ORIGIN
        1 taacggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
       61 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
      121 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
      181 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
      241 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
      301 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      361 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
      421 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
      481 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
      541 ggtgatggtt cacgtaccta gaagttccta ttccgaagtt cctattctct agaaagtata
      601 ggaacttcct tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
      661 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
      721 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
      781 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
      841 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
      901 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
      961 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     1021 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     1081 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     1141 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     1201 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     1261 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     1321 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     1381 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     1441 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     1501 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     1561 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     1621 ccgagggcaa aggaatagca cgtactacga gatttcgatt ccaccgccgc cttctatgaa
     1681 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     1741 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     1801 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     1861 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     1921 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     1981 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2041 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2101 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2161 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2221 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2281 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2341 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2401 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2461 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2521 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2581 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2641 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2701 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2761 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2821 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2881 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2941 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3001 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3061 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3121 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3181 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3241 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3301 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3361 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3421 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3481 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3541 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3601 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3661 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3721 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3781 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3841 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3901 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3961 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4021 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4081 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     4141 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     4201 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     4261 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     4321 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     4381 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     4441 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     4501 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     4561 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     4621 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     4681 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     4741 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     4801 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     4861 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta
     4921 tcagtgatag agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc
     4981 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
     5041 gatccagcct ccggactcta gcgtttaaac ttaagctgca gaagttggtc gtgaggcact
     5101 gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg
     5161 tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac
     5221 tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta aggctagagt
     5281 acttaatacg actcactata ggctagcctc gagaattcac gcgtggtacc tctagagtcg
     5341 acccgggatg gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc
     5401 cactggtgac atgttcgtgt tcctggtgct cctgcctctg gtgagcagcc agtgcgtgaa
     5461 cctgaccacc cgaacccagc tcccaccagc ctacaccaac agctttacac ggggcgtgta
     5521 ctaccctgac aaggtgttca gatctagcgt cctgcacagc actcaggacc tcttcctgcc
     5581 gttcttcagc aacgtgacat ggttccacgc catccacgtg agcggcacaa acggaaccaa
     5641 gcggtttgat aaccccgtcc tgccattcaa tgatggagtt tacttcgcca gtaccgagaa
     5701 gagtaacatc atccggggct ggatcttcgg caccaccctg gatagcaaaa cacagagcct
     5761 cctgatcgtg aacaatgcca cgaacgtcgt gatcaaggtg tgcgagttcc agttttgcaa
     5821 tgatcctttc ctgggtgtgt actaccacaa gaacaacaag agctggatgg aaagcgagtt
     5881 cagagtctac agcagcgcca acaactgcac attcgagtac gtctctcagc cttttctgat
     5941 ggaccttgag gggaaacaag gcaacttcaa gaacctgaga gaattcgtgt tcaagaacat
     6001 cgacggctac ttcaaaatct actccaagca cacacccatc aacctggtcc gggacctccc
     6061 tcagggcttc agcgccctgg aacccctggt cgacctgccc ataggcatca acataacgcg
     6121 gttccaaacc ctgctggccc tgcatagatc ctacctgact cctggcgaca gcagcagcgg
     6181 atggaccgcc ggagctgcag cctactatgt gggctacctg caacctagaa ccttcctgct
     6241 gaagtacaac gagaacggca caatcacaga cgccgtcgac tgcgccctgg accctctctc
     6301 tgagacaaag tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa
     6361 cttccgggtg cagcctacag agagcatcgt gcgatttcca aacattacca acctctgccc
     6421 cttcggcgag gtgtttaacg ccacaagatt tgcctccgtt tacgcctgga atagaaagag
     6481 aatcagcaat tgtgtggccg actactccgt gctgtataac agcgcctctt tcagcacctt
     6541 caagtgctac ggcgtttccc caacaaagct gaatgacctg tgcttcacca acgtgtacgc
     6601 cgactccttc gtaattagag gcgatgaggt gcggcagatc gcaccaggcc agaccggtaa
     6661 gatcgctgac tacaactata agctgcctga tgattttaca ggctgcgtga tcgcctggaa
     6721 ctctaacaac ctggatagca aggtgggcgg caactacaac tacctgtacc ggctgtttcg
     6781 caagtctaac ctgaaacctt tcgagagaga catctccaca gagatctacc aggccggttc
     6841 tacaccttgt aacggggtgg aaggcttcaa ctgttacttc cctctgcaaa gctacggctt
     6901 ccagcctacc aatggagtcg gctaccagcc ataccgggtg gtcgtgctgt ccttcgagtt
     6961 actccacgcc cccgccaccg tctgcggtcc taagaagtcc accaatctgg ttaagaacaa
     7021 atgcgtgaac ttcaacttca acggcctgac cgggaccggc gtgctgaccg aaagcaacaa
     7081 aaagttcctc cccttccagc agttcggccg tgatatcgct gacaccacag atgccgtcag
     7141 agatccacag accctggaaa tcctggatat tacaccctgc tccttcggag gagtttctgt
     7201 gatcaccccc gggaccaata ccagcaacca ggtggctgtg ctgtaccaag gtgttaactg
     7261 caccgaggtt cctgtggcca tccacgccga tcagctgaca cctacttgga gagtgtactc
     7321 cactggctcc aatgtgttcc agaccagggc cggatgtctg atcggcgccg agcacgtgaa
     7381 taacagttac gagtgcgaca tccctatcgg cgccggcatc tgtgccagct accagaccca
     7441 gacaaacagc cctgggtctg cttcctctgt agctagccag agcatcatcg cctacaccat
     7501 gagcctgggc gcagagaaca gcgtggccta ttccaacaac tctatcgcca ttcccaccaa
     7561 ctttacaatt agcgtcacaa cagagatcct gcccgtgagc atgaccaaga ccagcgtgga
     7621 ctgtacaatg tacatctgtg gcgacagcac tgaatgcagc aacctgctgc tgcaatacgg
     7681 ctccttttgc acccaactga accgggcgct gaccggaatc gccgtggaac aggacaaaaa
     7741 tacccaggag gtgttcgccc aagtgaagca gatctacaag accccaccta tcaaggactt
     7801 cggcggcttt aactttagcc agattctccc tgatccttct aagcctagca agcggagccc
     7861 tatcgaggat ctgctgttca acaaggtcac cctggccgat gccggcttta tcaaacagta
     7921 tggcgattgc ctgggcgaca tagccgccag agatctgatc tgcgcccaga aattcaacgg
     7981 cctgacagtt ctcccacctc tgctgaccga cgagatgatc gctcagtaca cctctgccct
     8041 gctggctggc accatcacat ctgggtggac atttggcgcc ggccccgccc tgcagatccc
     8101 ctttcccatg cagatggcct atagattcaa cggaatcggc gtgacccaga acgtgctgta
     8161 tgaaaaccag aagctgatcg ctaaccagtt caattctgcc atcggcaaga tccaggactc
     8221 cctctcctct acccccagcg ccctgggcaa actgcaggac gtggtgaatc agaacgccca
     8281 agccctgaac accctggtga agcagctcag cagcaatttt ggcgccatca gctctgtgct
     8341 gaacgatatc ctgtctagac tggaccctcc agaagccgaa gtccagatcg atagactgat
     8401 cacaggcaga ctgcagtccc tgcaaaccta cgtgacccaa cagctgatca gggccgctga
     8461 aataagagcc agcgccaatc tcgccgctac caagatgtcc gagtgtgtgc tgggacagtc
     8521 taaacgcgtt gacttctgcg gcaaaggcta tcacctgatg agcttccccc agaggtggca
     8581 ggtggaaagt gaaacgtgat ttcatgcgtc attttgaaca ttttgtaaat cttatttaat
     8641 aatgtgtgcg gcaattcaca tttaatttat gaatgttttc ttaacatcgc ggcaactcaa
     8701 gaaacggcag gttcggatct tagctactag agaaagagga gaaatactag atgcgtaaag
     8761 gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt gatgtcaacg
     8821 gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt aaactgacgc
     8881 tgaagttcat ctgtactact ggtaaactgc cggttccttg gccgactctg gtaacgacgc
     8941 tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag catgacttct
     9001 tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt aaggatgacg
     9061 gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta aaccgcattg
     9121 agctgaaagg cattgacttt aaagaggacg gcaatatcct gggccataag ctggaataca
     9181 attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc attaaagcga
     9241 attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat cactaccagc
     9301 aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat ctgagcacgc
     9361 aaagcgttct gtctaaagat ccgaacgaga aacgcgatca tatggttctg ctggagttcg
     9421 taaccgcagc gggcatcacg catggtatgg atgaactgta caaatgacca ggcatcaaat
     9481 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     9541 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatacacc
     9601 tgccaccgca gggatccgga tacatccccg aggcccccag agatggccag gcctacgtgc
     9661 ggaaggacgg cgagtgggta ctgctgagca cattcctggg cagatccgac tacaaagacc
     9721 atgacggtga ttataaagat catgacatcg attacaagga tgacgatgac aagagcgcct
     9781 ggtcccaccc ccagttcgag aagggatccc tggaggtgct gttccagggc ccaggcggcg
     9841 gcggtagtgg agggggcgga tctggcggct cagctgctgt gggccaggac acgcaggagg
     9901 tcatcgtggt gccacactcc ttgtcccttt aaggtggtgg tgatctcagc catcctggcc
     9961 ctggtggtgc tcaccatcat ctcccttatc atcctcatca tgctttggca gaagaagcca
    10021 cgt
//
