LOCUS       Exported                2477 bp DNA     circular SYN 15-JUN-2021
DEFINITION  YTK001 Part 1 (6P-D614G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly-characterized Yeast Toolkit for Modular, Multi-part 
            Assembly.
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 17, 2021 from SnapGene Viewer 5.3.0
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          20..816
                     /label=Part 1
                     /note="/created_by=moham"
                     /note="/modified_by=moham"
     source          20..425
                     /label=Part 1a
                     /note="/created_by=moham"
     source          430..816
                     /label=Part 1b
                     /note="/created_by=moham"
     misc_feature    5..11
                     /label=AarI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CACCTGC(4/8)"
                     /note="/annotation_group=CACCTGC"
     misc_feature    16..19
                     /label=Part-1 5' Overhang
                     /label=nonstandard type: overhang
                     /note="/created_by=moham"
                     /note="/modified_by=moham"
     CDS             20..816
                     /codon_start=1
                     /label=SARS-CoV-2 Spike Protein
                     /note="/Original_Translation=MFVFLVLLPLVSSQCVNLTTRTQLPPAYTN
                     SFTRG 
                     VYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFA 
                     STEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKS 
                     WMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTP 
                     INLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYV 
                     GYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTES 
                     IVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVS 
                     PTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNL 
                     DSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQP 
                     TNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNK 
                     KFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDV 
                     NCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICAS 
                     YQTQTNSPGSASSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSM 
                     TKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIY 
                     KTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAAR 
                     DLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYR 
                     FNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLV 
                     KQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRAS 
                     ANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAP 
                     AICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTV 
                     YDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNE 
                     SLIDLQELGKYEQ"
                     /note="/modified_by=moham"
                     /translation="MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRS
                     SVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGW
                     IFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSA
                     NNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSA
                     LEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAY"
     gene            98..816
                     /label=NTD
                     /note="/created_by=moham"
     misc_feature    426..429
                     /label=Part 1a 3' Overhang
                     /label=nonstandard type: overhang
                     /note="/created_by=moham"
                     /note="/modified_by=moham"
     misc_feature    426..429
                     /label=Part 1b 5' Overhang
                     /label=nonstandard type: overhang
                     /note="/created_by=moham"
                     /note="/modified_by=moham"
     misc_feature    529..534
                     /label=Esp3I, BsmBI
                     /note="Geneious type: restriction site"
                     /note="/Methylation=Mixed Methylation"
                     /note="/Recognition_pattern=CGTCTC(1/5)"
                     /note="/annotation_group=CGTCTC"
     primer_bind     749..771
                     
     misc_feature    817..820
                     /label=Part-1 3' Overhang
                     /label=nonstandard type: overhang
                     /note="/created_by=moham"
                     /note="/modified_by=moham"
     misc_feature    complement(825..831)
                     /label=AarI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CACCTGC(4/8)"
                     /note="/annotation_group=CACCTGC"
     terminator      842..876
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(947..1606)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1521..1540
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1788..2376
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2277..2296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggcacctg ccacctgaca tgttcgtgtt cctggtgctc ctgcctctgg tgagcagcca
       61 gtgcgtgaac ctgaccaccc gaacccagct cccaccagcc tacaccaaca gctttacacg
      121 gggcgtgtac taccctgaca aggtgttcag atctagcgtc ctgcacagca ctcaggacct
      181 cttcctgccg ttcttcagca acgtgacatg gttccacgcc atccacgtga gcggcacaaa
      241 cggaaccaag cggtttgata accccgtcct gccattcaat gatggagttt acttcgccag
      301 taccgagaag agtaacatca tccggggctg gatcttcggc accaccctgg atagcaaaac
      361 acagagcctc ctgatcgtga acaatgccac gaacgtcgtg atcaaggtgt gcgagttcca
      421 gttttgcaat gatcctttcc tgggtgtgta ctaccacaag aacaacaaga gctggatgga
      481 aagcgagttc agagtctaca gcagcgccaa caactgcaca ttcgagtacg tctctcagcc
      541 ttttctgatg gaccttgagg ggaaacaagg caacttcaag aacctgagag aattcgtgtt
      601 caagaacatc gacggctact tcaaaatcta ctccaagcac acacccatca acctggtccg
      661 ggacctccct cagggcttca gcgccctgga acccctggtc gacctgccca taggcatcaa
      721 cataacgcgg ttccaaaccc tgctggccct gcatagatcc tacctgactc ctggcgacag
      781 cagcagcgga tggaccgccg gagctgcagc ctactatgtg ggtggcaggt ggaccagacc
      841 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
      901 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
      961 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     1021 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     1081 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     1141 aactggtgaa actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt
     1201 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     1261 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     1321 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     1381 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     1441 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     1501 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     1561 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     1621 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     1681 gaaagttgga acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt
     1741 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1801 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1861 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1921 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1981 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2041 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2101 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2161 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2221 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2281 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2341 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2401 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2461 tctgtggata accgtag
//
