LOCUS       Exported                9445 bp DNA     circular SYN 11-AUG-2021
DEFINITION  SARS-CoV-2 S B.1.1.248 .
ACCESSION   urn.local...ek-d0c4vw8
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 17, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9445
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      42..277
                     /label=BGH-PA terminator
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide 
                     similarity<br>Alternative_Match=98.31%; 
                     Adjusted-Interval-0:0/0; BGH-PA (<a 
                     href=""urn:local:.:9zo-bzmwvsn"">JPF0509</a>)<br>Alternati 
                     ve_Match=98.31%; Adjusted-Interval-0:0/0; BGH-PA (<a 
                     href=""urn:local:.:9ww-bzmwvr7"">ARB341</a>)<br>Alternativ 
                     e_Match=98.31%; Adjusted-Interval-0:0/0; BGH-PA (<a 
                     href=""urn:local:.:9yc-bzmwvrt"">ARB367</a>)<br>Alternativ 
                     e_Match=98.31%; Adjusted-Interval-0:0/0; BGH-PA (<a 
                     href=""urn:local:.:9yo-bzmwvs4"">ARB387</a>)<br>Alternativ 
                     e_Match=98.31%; Adjusted-Interval-0:0/0; BGH-PA (<a 
                     href=""urn:local:.:9x4-bzmwvr9"">ARB343</a>)<br>Alternativ 
                     e_Match=98.31%; Adjusted-Interval-0:0/0; BGH-PA (<a 
                     href=""urn:local:.:a1s-bzmwvtt"">JPF0536b</a>)<br>Alternat 
                     ive_Match=98.31%; Adjusted-Interval-0:0/0; BGH-PA (<a 
                     href=""urn:local:.:a10-bzmwvtc"">JPF0521</a>)<br>Alternati 
                     ve_Match=98.31%; Adjusted-Interval-0:0/0; BGH-PA (<a 
                     href=""urn:local:.:a0g-bzmwvt2"">JPF0516</a>)<br>Alternati 
                     ve_Match=98.31%; Adjusted-Interval-0:0/0; BGH-PA (<a 
                     href=""urn:local:.:9y8-bzmwvrr"">ARB366</a>)<br>ApEinfo_fw 
                     dcolor=#c7b0e3<br>ApEinfo_revcolor=#c7b0e3<br>Primary_Matc 
                     h=98.31%; Adjusted-Interval-0:0/0; BGH-PA (<a 
                     href=""urn:local:.:a00-bzmwvst"">JPF0512</a>)<br>Transferr 
                     ed_From=JPF0512<br>Transferred_Similarity=98.31%"
     rep_origin      complement(575..608)
                     /direction=LEFT
                     /label=FRT site
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide 
                     similarity<br>ApEinfo_fwdcolor=#9A969B<br>ApEinfo_graphicf 
                     ormat=arrow_data {{0 1 2 0 0 -1} {} 
                     0}<br>ApEinfo_revcolor=#9A969B<br>Primary_Match=89.47%; 
                     Adjusted-Interval-0:0/0; FRT site (<a 
                     href=""urn:local:.:910-bzmvb6e"">pYTK082</a>)<br>Transferr 
                     ed_From=pYTK082<br>Transferred_Similarity=89.47%"
                     /note="/snapgene_direction=LEFT"
     CDS             610..1638
                     /codon_start=1
                     /gene="<i>aph(4)-Ia</i>"
                     /product="aminoglycoside phosphotransferase from <i>E.
                     coli</i>"
                     /label=HygR
                     /note="confers resistance to hygromycin<br>Derived using 
                     Geneious Prime 2020.1.2 'Annotate from Database' based on 
                     nucleotide 
                     similarity<br>Adjusted_Transferred_Similarity=99.42%<br>Al 
                     ternative_Match=100.00%; Adjusted-Interval-0:6/0 (adjusted 
                     to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:v-c0u7dc2"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:6/0 (adjusted to 99.42%; 
                     Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:973-bzmvybu"">pcDNA-FRT-TO-intron</a>)< 
                     br>Alternative_Match=100.00%; Adjusted-Interval-0:6/0 
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:3i-c0uqp26"">pcDNA5-intron-Spike-3XFLAG 
                     -TM-2A-EGFP</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:6/0 (adjusted to 99.42%; 
                     Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:3f-c45uttj"">pcDNA5_TET_intron_HA_NLS_E 
                     co2_EGFP</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:6/0 (adjusted to 99.42%; 
                     Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:2r-c0uhwai"">RFP-GFP 
                     reporter_pcDNA5-FRT-TO-intron_v1</a>)<br>Alternative_Match 
                     =100.00%; Adjusted-Interval-0:6/0 (adjusted to 99.42%; 
                     Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:9ft-bzmwl6g"">pcDNA5-intron_HA_NLS_Eco1 
                     _FLAG</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:6/0 (adjusted to 99.42%; 
                     Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:97c-bzmvyby"">pcDNA5 Reporter (RFP GFP) 
                     with hPGK promoter (replaced 
                     CMV)</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:6/0 (adjusted to 99.42%; 
                     Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:9fn-bzmwl6e"">pcDNA5-intron_HA_NLS_Eco1 
                     _EGFP</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:6/0 (adjusted to 99.42%; 
                     Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:9fk-bzmwl6d"">pcDNA5-intron_HA_NLS_Eco1 
                     </a>)<br>Primary_Match=100.00%; Adjusted-Interval-0:6/0 
                     (adjusted to 99.42%; Adjusted-Interval-0:0/0); HygR (<a 
                     href=""urn:local:.:u-c2oyjr4"">pcDNA5_TET_intron_HA_NLS_Ec 
                     o1_EGFP</a>)<br>Transferred_From=pcDNA5_TET_intron_HA_NLS_ 
                     Eco1_EGFP<br>Transferred_Similarity=100.00%<br>Transferred 
                     _Translation=KKPELTATSVEKFLIEKFDSVSDLMQLSEGEE 
                     SRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCI 
                     SRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRD 
                     FICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTD 
                     NGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAY 
                     MLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVE 
                     VLADSGNRRPSTRPRAKE*"
                     /translation="LAKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     misc_feature    675..680
                     /label=Esp3I, BsmBI
                     /note="Geneious type: restriction
                     site<br>Methylation=Mixed 
                     Methylation<br>Recognition_pattern=CGTCTC(1/5)<br>annotati 
                     on_group=CGTCTC"
     polyA_signal    1768..1889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal<br>Derived using
                     Geneious Prime 2020.1.2 'Annotate from Database' based on 
                     nucleotide similarity<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; SV40 poly(A) signal (<a 
                     href=""urn:local:.:9e4-bzmwint"">Exported</a>)<br>Alternat 
                     ive_Match=100.00%; Adjusted-Interval-0:0/0; SV40 poly(A) 
                     signal (<a 
                     href=""urn:local:.:7t-c2wj41q"">Lenti__IVT_gBlock_msr-msd_ 
                     (Wt)</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; SV40 poly(A) signal (<a 
                     href=""urn:local:.:87-c2wj420"">Lenti_IVT_gBlock_msr-msd_( 
                     TurboRFP_repair)</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; SV40 poly(A) signal (<a 
                     href=""urn:local:.:8f-c2wj425"">Lenti_sgRNA_sfGFP-DO_no_Sp 
                     yCas9</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; SV40 poly(A) signal (<a 
                     href=""urn:local:.:9ar-bzmvydt"">Exported</a>)<br>Alternat 
                     ive_Match=100.00%; Adjusted-Interval-0:0/0; SV40 poly(A) 
                     signal (<a 
                     href=""urn:local:.:7z-c2wj41v"">Lenti_IVT_gBlock_msr-msd_( 
                     TurboRFP_repair)_HH_and_HDV</a>)<br>Alternative_Match=100. 
                     00%; Adjusted-Interval-0:0/0; SV40 poly(A) signal (<a 
                     href=""urn:local:.:9ia-bzmwl77"">Exported</a>)<br>Alternat 
                     ive_Match=100.00%; Adjusted-Interval-0:0/0; SV40 poly(A) 
                     signal (<a 
                     href=""urn:local:.:9az-bzmvydy"">Exported</a>)<br>Alternat 
                     ive_Match=100.00%; Adjusted-Interval-0:0/0; SV40 poly(A) 
                     signal (<a 
                     href=""urn:local:.:9an-bzmvydr"">Exported</a>)<br>Primary_ 
                     Match=100.00%; Adjusted-Interval-0:0/0; SV40 poly(A) signal
                     (<a 
                     href=""urn:local:.:9av-bzmvydw"">Exported</a>)<br>Transfer 
                     red_From=Exported<br>Transferred_Similarity=100.00%"
     promoter        complement(1942..2016)
                     /label=trc promoter
                     /note="hybrid of the trp and lac promoters that is
                     stronger than the lac promoter<br>Derived using Geneious 
                     Prime 2020.1.2 'Annotate from Database' based on nucleotide
                     similarity<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; trc (<a 
                     href=""urn:local:.:eh-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 4</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; trc (<a 
                     href=""urn:local:.:d-c1aa132"">SrtA-PDGFR 
                     Tm</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; trc (<a 
                     href=""urn:local:.:2t-c1pusju"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; trc (<a 
                     href=""urn:local:.:18-c1ldurm"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; trc (<a 
                     href=""urn:local:.:1o-c0u8hgs"">SARS-CoV-2_S-2P</a>)<br>Al 
                     ternative_Match=100.00%; Adjusted-Interval-0:0/0; trc (<a 
                     href=""urn:local:.:y-c0v8hwh"">SARS-CoV-2_S-2P_Cell Surface
                     1</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; trc (<a 
                     href=""urn:local:.:eg-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; trc (<a 
                     href=""urn:local:.:ef-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; trc (<a 
                     href=""urn:local:.:ee-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)<br>Primary_Match=100.00%; 
                     Adjusted-Interval-0:0/0; trc (<a 
                     href=""urn:local:.:x-c34iv9l"">SARS-CoV-2_S-2P</a>)<br>Tra 
                     nsferred_From=SARS-CoV-2_S-2P<br>Transferred_Similarity=10 
                     0.00%<br>X-Primary_Match=82.67%; Adjusted-Interval-0:0/0; 
                     <a 
                     href=""urn:local:.:1oh-bzc80e2"">trc</a><br>X-Transferred_ 
                     From=trc<br>X-Transferred_Similarity=82.67%"
     regulatory      2031..2052
                     /regulatory_class="other"
                     /standard_name="CAP BS"
                     /label=CAP BS
                     /note="catabolite activator protein binding
                     site<br>Derived using Geneious Prime 2020.1.2 'Annotate 
                     from Database' based on nucleotide 
                     similarity<br>Alternative_Match=95.45%; 
                     Adjusted-Interval-0:0/0; CAP BS (<a 
                     href=""urn:local:.:11-c4pu1xm"">pMAZ-SK_795</a>)<br>Altern 
                     ative_Match=100.00%; Adjusted-Interval-0:2/-2; CAP BS (<a 
                     href=""urn:local:.:37-c0ul19s"">SARS-CoV-2_S-2P translation
                     2</a>)<br>Primary_Match=95.45%; Adjusted-Interval-0:0/0; 
                     CAP BS (<a 
                     href=""urn:local:.:y-c4pu1xk"">pMAZ-SK_794</a>)<br>Transfe 
                     rred_From=pMAZ-SK_794<br>Transferred_Similarity=95.45%"
     rep_origin      2340..2928
                     /label=ori
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide 
                     similarity<br>Primary_Match=100.00%; 
                     Adjusted-Interval-0:0/0; ori (<a 
                     href=""urn:local:.:w-bzpsocc"">EZeo 
                     vector_Simon</a>)<br>Transferred_From=EZeo 
                     vector_Simon<br>Transferred_Similarity=100.00%"
     terminator      complement(3005..3101)
                     /label=AmpR Terminator
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide 
                     similarity<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; AmpR Terminator (<a 
                     href=""urn:local:.:a34-bzmwvue"">MTK0_031</a>)<br>Alternat 
                     ive_Match=100.00%; Adjusted-Interval-0:0/0; AmpR Terminator
                     (<a 
                     href=""urn:local:.:9ik-bzmwsep"">MTK0_001</a>)<br>Alternat 
                     ive_Match=100.00%; Adjusted-Interval-0:0/0; AmpR Terminator
                     (<a 
                     href=""urn:local:.:9kc-bzmwsfg"">MTK0_025</a>)<br>Alternat 
                     ive_Match=100.00%; Adjusted-Interval-0:0/0; AmpR Terminator
                     (<a 
                     href=""urn:local:.:9x4-bzmwvr9"">ARB343</a>)<br>Alternativ 
                     e_Match=100.00%; Adjusted-Interval-0:0/0; AmpR Terminator 
                     (<a 
                     href=""urn:local:.:9xk-bzmwvrf"">ARB347</a>)<br>Alternativ 
                     e_Match=100.00%; Adjusted-Interval-0:0/0; AmpR Terminator 
                     (<a 
                     href=""urn:local:.:9jw-bzmwsfa"">MTK0_021</a>)<br>Alternat 
                     ive_Match=100.00%; Adjusted-Interval-0:0/0; AmpR Terminator
                     (<a 
                     href=""urn:local:.:aq-c2wk6f6"">TU3ER</a>)<br>Alternative_ 
                     Match=100.00%; Adjusted-Interval-0:0/0; AmpR Terminator (<a
                     href=""urn:local:.:92g-bzmvb6x"">pYTK095</a>)<br>Alternati 
                     ve_Match=100.00%; Adjusted-Interval-0:0/0; AmpR Terminator 
                     (<a 
                     href=""urn:local:.:9x8-bzmwvrb"">ARB344</a>)<br>ApEinfo_fw 
                     dcolor=#f58a5e<br>ApEinfo_revcolor=#f58a5e<br>Primary_Matc 
                     h=100.00%; Adjusted-Interval-0:0/0; AmpR Terminator (<a 
                     href=""urn:local:.:9ww-bzmwvr7"">ARB341</a>)<br>Transferre 
                     d_From=ARB341<br>Transferred_Similarity=100.00%"
     CDS             complement(3099..3959)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics<br>Derived using Geneious Prime 
                     2020.1.2 'Annotate from Database' based on nucleotide 
                     similarity<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; AmpR (<a 
                     href=""urn:local:.:2c-bzrqycv"">pIF508_msr-msd_(TurboRFP_r 
                     epair)</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; AmpR (<a 
                     href=""urn:local:.:y-c2vthvs"">pDisplay-mSA-EGFP-TM</a>)<b 
                     r>Alternative_Match=100.00%; Adjusted-Interval-0:0/0; AmpR 
                     (<a href=""urn:local:.:d-c1aa132"">SrtA-PDGFR 
                     Tm</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; AmpR (<a 
                     href=""urn:local:.:7n-c8vy8oc"">pDisplay-mSA-EGFP-TM</a>)< 
                     br>Alternative_Match=100.00%; Adjusted-Interval-0:0/0; AmpR
                     (<a href=""urn:local:.:1y-c61zrev"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; AmpR (<a 
                     href=""urn:local:.:26-bzrqycr"">pIF508_msr-msd_(TurboRFP_r 
                     epair)</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; AmpR (<a 
                     href=""urn:local:.:1x-c61zreu"">pDisplay-SS47 
                     (86)-EGFP-TM</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; AmpR (<a 
                     href=""urn:local:.:1x-c7wp0k7"">pDisplay-SS47 
                     (86)-EGFP-TM</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; AmpR (<a 
                     href=""urn:local:.:19-c5kw4c2"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)<br>Primary_Match=100.00%; 
                     Adjusted-Interval-0:0/0; AmpR (<a 
                     href=""urn:local:.:6a-c8vxg0z"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)<br>Transferred_From=pDisplay-SS47 
                     (87)-EGFP-TM<br>Transferred_Similarity=100.00%<br>Transfer 
                     red_Translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVK 
                     DAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLG 
                     RRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTA 
                     FLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLI 
                     DWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGS 
                     QATMDERNRQIAEIGASLIKHW"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(3239..3244)
                     /label=BsaI
                     /note="Geneious type: restriction 
                     site<br>Methylation=Dcm:variable, CpG:variable, 
                     EcoBI:cut<br>Recognition_pattern=GGTCTC(1/5)<br>annotation 
                     _group=GGTCTC"
     promoter        complement(3960..4090)
                     /label=AmpR Promoter
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide 
                     similarity<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; AmpR Promoter (<a 
                     href=""urn:local:.:a34-bzmwvue"">MTK0_031</a>)<br>Alternat 
                     ive_Match=100.00%; Adjusted-Interval-0:0/0; AmpR Promoter 
                     (<a 
                     href=""urn:local:.:9ik-bzmwsep"">MTK0_001</a>)<br>Alternat 
                     ive_Match=100.00%; Adjusted-Interval-0:0/0; AmpR Promoter 
                     (<a 
                     href=""urn:local:.:9kc-bzmwsfg"">MTK0_025</a>)<br>Alternat 
                     ive_Match=100.00%; Adjusted-Interval-0:0/0; AmpR Promoter 
                     (<a 
                     href=""urn:local:.:9x4-bzmwvr9"">ARB343</a>)<br>Alternativ 
                     e_Match=100.00%; Adjusted-Interval-0:0/0; AmpR Promoter (<a
                     href=""urn:local:.:9xk-bzmwvrf"">ARB347</a>)<br>Alternativ 
                     e_Match=100.00%; Adjusted-Interval-0:0/0; AmpR Promoter (<a
                     href=""urn:local:.:9jw-bzmwsfa"">MTK0_021</a>)<br>Alternat 
                     ive_Match=100.00%; Adjusted-Interval-0:0/0; AmpR Promoter 
                     (<a 
                     href=""urn:local:.:aq-c2wk6f6"">TU3ER</a>)<br>Alternative_ 
                     Match=100.00%; Adjusted-Interval-0:0/0; AmpR Promoter (<a 
                     href=""urn:local:.:92g-bzmvb6x"">pYTK095</a>)<br>Alternati 
                     ve_Match=100.00%; Adjusted-Interval-0:0/0; AmpR Promoter 
                     (<a 
                     href=""urn:local:.:9x8-bzmwvrb"">ARB344</a>)<br>ApEinfo_fw 
                     dcolor=#f58a5e<br>ApEinfo_revcolor=#f58a5e<br>Primary_Matc 
                     h=100.00%; Adjusted-Interval-0:0/0; AmpR Promoter (<a 
                     href=""urn:local:.:9ww-bzmwvr7"">ARB341</a>)<br>Transferre 
                     d_From=ARB341<br>Transferred_Similarity=100.00%"
     enhancer        4330..4709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early
                     enhancer<br>Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide 
                     similarity<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; CMV enhancer (<a 
                     href=""urn:local:.:9bv-bzmwcmo"">Lenti_FnCas9_sfGFP 
                     DO</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; CMV enhancer (<a 
                     href=""urn:local:.:72-c0q55wp"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9 - 
                     KJ_T0505_IVT_gBlock_msr-msd_(Wt)</a>)<br>Alternative_Match 
                     =100.00%; Adjusted-Interval-0:0/0; CMV enhancer (<a 
                     href=""urn:local:.:9f6-bzmwl69"">Lenti_TET_HA_NLS_EcoI_EGF 
                     P</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; CMV enhancer (<a 
                     href=""urn:local:.:9c9-bzmwcmt"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; CMV enhancer (<a 
                     href=""urn:local:.:9ea-bzmwl5z"">3XFLAG-NLS-RT</a>)<br>Alt 
                     ernative_Match=100.00%; Adjusted-Interval-0:0/0; CMV 
                     enhancer (<a 
                     href=""urn:local:.:1l-c0q1vfs"">Lenti_sgRNA_sfGFP-DO_no 
                     SpyCas9</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; CMV enhancer (<a 
                     href=""urn:local:.:96x-bzmvybt"">Pax2</a>)<br>Alternative_ 
                     Match=100.00%; Adjusted-Interval-0:0/0; CMV enhancer (<a 
                     href=""urn:local:.:9e7-bzmwl5y"">3XFLAG-NLS-RT-EGFP</a>)<b 
                     r>Alternative_Match=100.00%; Adjusted-Interval-0:0/0; CMV 
                     enhancer (<a 
                     href=""urn:local:.:973-bzmvybu"">pcDNA-FRT-TO-intron</a>)< 
                     br>Primary_Match=100.00%; Adjusted-Interval-0:0/0; CMV 
                     enhancer (<a 
                     href=""urn:local:.:9em-bzmwl63"">HA-NLS-RT-EGFP</a>)<br>Tr 
                     ansferred_From=HA-NLS-RT-EGFP<br>Transferred_Similarity=10 
                     0.00%"
     promoter        4710..4913
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter<br>Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide 
                     similarity<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; CMV promoter (<a 
                     href=""urn:local:.:95r-bzmvybf"">mNeptune2 CRISPEY msr 
                     msd</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; CMV promoter (<a 
                     href=""urn:local:.:9av-bzmvydw"">Exported</a>)<br>Alternat 
                     ive_Match=100.00%; Adjusted-Interval-0:0/0; CMV promoter 
                     (<a href=""urn:local:.:95x-bzmvybg"">mNeptune2 
                     CRISPEY_81mer_sgRNA1</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; CMV promoter (<a 
                     href=""urn:local:.:6a-c8vxg0z"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; CMV promoter (<a 
                     href=""urn:local:.:9ea-bzmwl5z"">3XFLAG-NLS-RT</a>)<br>Alt 
                     ernative_Match=100.00%; Adjusted-Interval-0:0/0; CMV 
                     promoter (<a 
                     href=""urn:local:.:96x-bzmvybt"">Pax2</a>)<br>Alternative_ 
                     Match=100.00%; Adjusted-Interval-0:0/0; CMV promoter (<a 
                     href=""urn:local:.:18-bzpsocj"">PX408 Francisella 
                     tularensis subsp. novicida 
                     Cas9</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; CMV promoter (<a 
                     href=""urn:local:.:9e7-bzmwl5y"">3XFLAG-NLS-RT-EGFP</a>)<b 
                     r>Alternative_Match=100.00%; Adjusted-Interval-0:0/0; CMV 
                     promoter (<a 
                     href=""urn:local:.:973-bzmvybu"">pcDNA-FRT-TO-intron</a>)< 
                     br>Primary_Match=100.00%; Adjusted-Interval-0:0/0; CMV 
                     promoter (<a 
                     href=""urn:local:.:9em-bzmwl63"">HA-NLS-RT-EGFP</a>)<br>Tr 
                     ansferred_From=HA-NLS-RT-EGFP<br>Transferred_Similarity=10 
                     0.00%"
     protein_bind    join(4915..4933,4936..4954)
                     /label=TetO
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide 
                     similarity<br>Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:1z8-bzlzoz0"">pMA7CR_2.0</a>)<br>Altern 
                     ative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:1z8-bzlzoz0"">pMA7CR_2.0</a>)<br>Altern 
                     ative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:1zc-bzlzoz3"">pMAZ-SK_sfGFP 
                     DO</a>)<br>Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:1zc-bzlzoz3"">pMAZ-SK_sfGFP 
                     DO</a>)<br>Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:1zc-bzlzoz3"">pMAZ-SK_sfGFP 
                     DO</a>)<br>Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:1zc-bzlzoz3"">pMAZ-SK_sfGFP 
                     DO</a>)<br>Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:i-c21rl6b"">pMAZ-SK_sfGFP 
                     DO</a>)<br>Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:i-c21rl6b"">pMAZ-SK_sfGFP 
                     DO</a>)<br>Alternative_Match=100.00%; tet operator (<a 
                     href=""urn:local:.:i-c21rl6b"">pMAZ-SK_sfGFP 
                     DO</a>)<br>Primary_Match=100.00%; Adjusted-Interval-0:0/0, 
                     Adjusted-Interval-1:0/0; TetO (<a 
                     href=""urn:local:.:ai-c2wk6ez"">pSK.102</a>)<br>Transferre 
                     d_From=pSK.102<br>Transferred_Similarity=100.00%<br>create 
                     d_by=User"
     misc_feature    complement(4994..4999)
                     /label=Esp3I, BsmBI
                     /note="Geneious type: restriction
                     site<br>Methylation=Mixed 
                     Methylation<br>Recognition_pattern=CGTCTC(1/5)<br>annotati 
                     on_group=CGTCTC"
     intron          5107..5239
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes<br>Derived using Geneious 
                     Prime 2020.1.2 'Annotate from Database' based on nucleotide
                     similarity<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; chimeric intron (<a 
                     href=""urn:local:.:9b3-bzmvye1"">Exported</a>)<br>Primary_ 
                     Match=100.00%; Adjusted-Interval-0:0/0; chimeric intron (<a
                     href=""urn:local:.:9ia-bzmwl77"">Exported</a>)<br>Transfer 
                     red_From=Exported<br>Transferred_Similarity=100.00%"
     misc_feature    complement(5138..5143)
                     /label=BsaI
                     /note="Geneious type: restriction 
                     site<br>Methylation=Dcm:variable, CpG:variable, 
                     EcoBI:cut<br>Recognition_pattern=GGTCTC(1/5)<br>annotation 
                     _group=GGTCTC"
     promoter        5284..5302
                     /label=T7 Promoter
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide 
                     similarity<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; T7 Promoter (<a 
                     href=""urn:local:.:9m-c2wj42l"">pKJ0006:_ES.entry_-_T7_Pro 
                     moter_RBS1_(T0434_T0435)</a>)<br>Alternative_Match=100.00% 
                     ; Adjusted-Interval-0:0/0; T7 Promoter (<a 
                     href=""urn:local:.:8n-c2wj429"">pKJ0001</a>)<br>Alternativ 
                     e_Match=100.00%; Adjusted-Interval-0:0/0; T7 Promoter (<a 
                     href=""urn:local:.:6y-c2wj41d"">KJ_T0417_msr_Eco1_</a>)<br 
                     >Alternative_Match=100.00%; Adjusted-Interval-0:0/0; T7 
                     promoter (<a 
                     href=""urn:local:.:9em-bzmwl63"">HA-NLS-RT-EGFP</a>)<br>Al 
                     ternative_Match=100.00%; Adjusted-Interval-0:0/0; T7 
                     promoter (<a 
                     href=""urn:local:.:1e-c4vgcq6"">KJ_T0630_IVT_gBlock_msr-ms 
                     d RFP repair </a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; T7 promoter (<a 
                     href=""urn:local:.:9hk-bzmwl6y"">Twin Strep SUMO_HA NLS 
                     Ec86</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; T7 promoter (<a 
                     href=""urn:local:.:97w-bzmvyc8"">pMBP-AsCas12a</a>)<br>Alt 
                     ernative_Match=100.00%; Adjusted-Interval-0:0/0; T7 
                     promoter (<a 
                     href=""urn:local:.:52-c0q53l2"">KJ_T0518_IVT_gBlock_msr-ms 
                     d_(Wt)_mutant_1</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; T7 promoter (<a 
                     href=""urn:local:.:6a-c8vxg0z"">pDisplay-SS47 
                     (87)-EGFP-TM</a>)<br>Primary_Match=100.00%; 
                     Adjusted-Interval-0:0/0; T7 Promoter (<a 
                     href=""urn:local:.:6r-c2wj41b"">KJ_T0417_msr_Eco1__-_mScar 
                     let-DO_(T0417/T0418)_-_KJ_T0418_msd_Eco1_</a>)<br>T0418)_- 
                     _KJ_T0418_msd_Eco1_""=<br>Transferred_From=T7 
                     Promoter<br>Transferred_From=""KJ_T0417_msr_Eco1__-_mScarl 
                     et-DO_(T0417<br>Transferred_Similarity=100.00%<br>Transfer 
                     red_Similarity=100.00%<br>created_by=moham"
     CDS             5348..5410
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     misc_feature    5407..5410
                     /label=Ligation
     CDS             5411..9034
                     /codon_start=1
                     /label=SARS-CoV-2 Spike Protein
                     /note="created_by=moham<br>modified_by=moham"
                     /translation="MFVFLVLLPLVSSQCVNFTNRTQLPSAYTNSFTRGVYYPDKVFRS
                     SVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGW
                     IFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNYPFLGVYYHKNNKSWMESEFRVYSSA
                     NNCTFEYVSQPFLMDLEGKQGNFKNLSEFVFKNIDGYFKIYSKHTPINLVRDLPQGFSA
                     LEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNE
                     NGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGE
                     VFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADS
                     FVIRGDEVRQIAPGQTGTIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRK
                     SNLKPFERDISTEIYQAGSTPCNGVKGFNCYFPLQSYGFQPTYGVGYQPYRVVVLSFEL
                     LHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAV
                     RDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRV
                     YSTGSNVFQTRAGCLIGAEYVNNSYECDIPIGAGICASYQTQTNSPGSASSVASQSIIA
                     YTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLL
                     LQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKP
                     SKRSPIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIA
                     QYTSALLAGTITSGWTFGAGPALQIPFPMQMAYRFNGIGVTQNVLYENQKLIANQFNSA
                     IGKIQDSLSSTPSALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDPPEA
                     EVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAAIKMSECVLGQSKRVDFCGKGYH
                     LMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQR
                     NFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDL
                     GDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQ"
     misc_feature    5462..5464
                     /label=L18F
     misc_feature    5468..5470
                     /label=T20N
     misc_feature    5486..5488
                     /label=P26S
     misc_feature    5822..5824
                     /label=D138Y
     misc_feature    5978..5980
                     /label=R190S
     misc_feature    6208..6211
                     /label=Ligation
     misc_feature    6659..6661
                     /label=K417T
     misc_feature    6860..6862
                     /label=E484K
     misc_feature    6911..6913
                     /label=N501Y
     misc_feature    6974..6977
                     /label=Ligation
     misc_feature    7373..7375
                     /label=H655Y
     misc_feature    7771..7774
                     /label=Ligation
     misc_feature    8489..8491
                     /label=T1027I
     misc_feature    8570..8573
                     /label=Ligation
     gene            9031..9034
                     /label=HR, CH, and CTD
                     /note="created_by=moham"
     misc_feature    9031..9034
                     /label=Ligation
     misc_feature    9044..9124
                     /label=Foldon
     CDS             9131..9196
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             9203..9226
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             9233..9256
                     /codon_start=1
                     /product="recognition and cleavage site for human
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     gene            9299..9445
                     /label=PDGFR-B TM domain
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide 
                     similarity<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; PDGFR-B TM domain (<a 
                     href=""urn:local:.:2t-c1pusju"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; PDGFR-B TM domain (<a 
                     href=""urn:local:.:18-c1ldurm"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; PDGFR-B TM domain (<a 
                     href=""urn:local:.:y-c0v8hwh"">SARS-CoV-2_S-2P_Cell Surface
                     1</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; PDGFR-B TM domain (<a 
                     href=""urn:local:.:eg-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 3</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; PDGFR-B TM domain (<a 
                     href=""urn:local:.:ef-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 2</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; PDGFR-B TM domain (<a 
                     href=""urn:local:.:ee-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 1</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; PDGFR-B TM domain (<a 
                     href=""urn:local:.:4y-c1pwmyb"">SARS-CoV-2_S-2P_Cell 
                     Surface 4</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; PDGFR-B TM domain (<a 
                     href=""urn:local:.:h-c9zxg2l"">Spike-hinge-TM 
                     extract</a>)<br>Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; PDGFR-B TM domain (<a 
                     href=""urn:local:.:n-c79snqp"">S-2P_86_TM</a>)<br>Primary_ 
                     Match=100.00%; Adjusted-Interval-0:0/0; PDGFR-B TM domain 
                     (<a href=""urn:local:.:eh-c30wztu"">SARS-CoV-2_S-2P_Cell 
                     Surface 4</a>)<br>Transferred_From=SARS-CoV-2_S-2P_Cell 
                     Surface 
                     4<br>Transferred_Similarity=100.00%<br>created_by=moham"
ORIGIN
        1 taacggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
       61 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
      121 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
      181 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
      241 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
      301 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      361 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
      421 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
      481 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
      541 ggtgatggtt cacgtaccta gaagttccta ttccgaagtt cctattctct agaaagtata
      601 ggaacttcct tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
      661 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
      721 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
      781 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
      841 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
      901 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
      961 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     1021 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     1081 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     1141 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     1201 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     1261 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     1321 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     1381 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     1441 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     1501 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     1561 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     1621 ccgagggcaa aggaatagca cgtactacga gatttcgatt ccaccgccgc cttctatgaa
     1681 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     1741 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     1801 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     1861 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     1921 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     1981 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2041 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2101 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2161 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2221 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2281 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2341 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2401 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2461 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2521 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2581 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2641 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2701 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2761 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2821 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2881 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2941 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3001 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3061 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3121 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3181 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3241 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3301 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3361 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3421 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3481 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3541 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3601 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3661 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3721 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3781 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3841 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3901 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3961 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4021 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4081 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     4141 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     4201 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     4261 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     4321 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     4381 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     4441 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     4501 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     4561 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     4621 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     4681 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     4741 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     4801 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     4861 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta
     4921 tcagtgatag agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc
     4981 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
     5041 gatccagcct ccggactcta gcgtttaaac ttaagctgca gaagttggtc gtgaggcact
     5101 gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg
     5161 tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac
     5221 tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta aggctagagt
     5281 acttaatacg actcactata ggctagcctc gagaattcac gcgtggtacc tctagagtcg
     5341 acccgggatg gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc
     5401 cactggtgac atgttcgtgt tcctggtgct cctgcctctg gtgagcagcc agtgcgtgaa
     5461 cttcaccaac cgaacccagc tcccatccgc ctacaccaac agctttacac ggggcgtgta
     5521 ctaccctgac aaggtgttca gatctagcgt cctgcacagc actcaggacc tcttcctgcc
     5581 gttcttcagc aacgtgacat ggttccacgc catccacgtg agcggcacaa acggaaccaa
     5641 gcggtttgat aaccccgtcc tgccattcaa tgatggagtt tacttcgcca gtaccgagaa
     5701 gagtaacatc atccggggct ggatcttcgg caccaccctg gatagcaaaa cacagagcct
     5761 cctgatcgtg aacaatgcca cgaacgtcgt gatcaaggtg tgcgagttcc agttttgcaa
     5821 ttaccctttc ctgggtgtgt actaccacaa gaacaacaag agctggatgg aaagcgagtt
     5881 cagagtctac agcagcgcca acaactgcac attcgagtac gtctctcagc cttttctgat
     5941 ggaccttgag gggaaacaag gcaacttcaa gaacctgtcc gaattcgtgt tcaagaacat
     6001 cgacggctac ttcaaaatct actccaagca cacacccatc aacctggtcc gggacctccc
     6061 tcagggcttc agcgccctgg aacccctggt cgacctgccc ataggcatca acataacgcg
     6121 gttccaaacc ctgctggccc tgcatagatc ctacctgact cctggcgaca gcagcagcgg
     6181 atggaccgcc ggagctgcag cctactatgt gggctacctg caacctagaa ccttcctgct
     6241 gaagtacaac gagaacggca caatcacaga cgccgtcgac tgcgccctgg accctctctc
     6301 tgagacaaag tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa
     6361 cttccgggtg cagcctacag agagcatcgt gcgatttcca aacattacca acctctgccc
     6421 cttcggcgag gtgtttaacg ccacaagatt tgcctccgtt tacgcctgga atagaaagag
     6481 aatcagcaat tgtgtggccg actactccgt gctgtataac agcgcctctt tcagcacctt
     6541 caagtgctac ggcgtttccc caacaaagct gaatgacctg tgcttcacca acgtgtacgc
     6601 cgactccttc gtaattagag gcgatgaggt gcggcagatc gcaccaggcc agaccggtac
     6661 catcgctgac tacaactata agctgcctga tgattttaca ggctgcgtga tcgcctggaa
     6721 ctctaacaac ctggatagca aggtgggcgg caactacaac tacctgtacc ggctgtttcg
     6781 caagtctaac ctgaaacctt tcgagagaga catctccaca gagatctacc aggccggttc
     6841 tacaccttgt aacggggtga agggcttcaa ctgttacttc cctctgcaaa gctacggctt
     6901 ccagcctacc tacggagtcg gctaccagcc ataccgggtg gtcgtgctgt ccttcgagtt
     6961 actccacgcc cccgccaccg tctgcggtcc taagaagtcc accaatctgg ttaagaacaa
     7021 atgcgtgaac ttcaacttca acggcctgac cgggaccggc gtgctgaccg aaagcaacaa
     7081 aaagttcctc cccttccagc agttcggccg tgatatcgct gacaccacag atgccgtcag
     7141 agatccacag accctggaaa tcctggatat tacaccctgc tccttcggag gagtttctgt
     7201 gatcaccccc gggaccaata ccagcaacca ggtggctgtg ctgtaccaag gtgttaactg
     7261 caccgaggtt cctgtggcca tccacgccga tcagctgaca cctacttgga gagtgtactc
     7321 cactggctcc aatgtgttcc agaccagggc cggatgtctg atcggcgccg agtacgtgaa
     7381 taacagttac gagtgcgaca tccctatcgg cgccggcatc tgtgccagct accagaccca
     7441 gacaaacagc cctgggtctg cttcctctgt agctagccag agcatcatcg cctacaccat
     7501 gagcctgggc gcagagaaca gcgtggccta ttccaacaac tctatcgcca ttcccaccaa
     7561 ctttacaatt agcgtcacaa cagagatcct gcccgtgagc atgaccaaga ccagcgtgga
     7621 ctgtacaatg tacatctgtg gcgacagcac tgaatgcagc aacctgctgc tgcaatacgg
     7681 ctccttttgc acccaactga accgggcgct gaccggaatc gccgtggaac aggacaaaaa
     7741 tacccaggag gtgttcgccc aagtgaagca gatctacaag accccaccta tcaaggactt
     7801 cggcggcttt aactttagcc agattctccc tgatccttct aagcctagca agcggagccc
     7861 tatcgaggat ctgctgttca acaaggtcac cctggccgat gccggcttta tcaaacagta
     7921 tggcgattgc ctgggcgaca tagccgccag agatctgatc tgcgcccaga aattcaacgg
     7981 cctgacagtt ctcccacctc tgctgaccga cgagatgatc gctcagtaca cctctgccct
     8041 gctggctggc accatcacat ctgggtggac atttggcgcc ggccccgccc tgcagatccc
     8101 ctttcccatg cagatggcct atagattcaa cggaatcggc gtgacccaga acgtgctgta
     8161 tgaaaaccag aagctgatcg ctaaccagtt caattctgcc atcggcaaga tccaggactc
     8221 cctctcctct acccccagcg ccctgggcaa actgcaggac gtggtgaatc agaacgccca
     8281 agccctgaac accctggtga agcagctcag cagcaatttt ggcgccatca gctctgtgct
     8341 gaacgatatc ctgtctagac tggaccctcc agaagccgaa gtccagatcg atagactgat
     8401 cacaggcaga ctgcagtccc tgcaaaccta cgtgacccaa cagctgatca gggccgctga
     8461 aataagagcc agcgccaatc tcgccgctat caagatgtcc gagtgtgtgc tgggacagtc
     8521 taaacgcgtt gacttctgcg gcaaaggcta tcacctgatg agcttccccc agagcgcgcc
     8581 gcacggcgtg gtgttcctgc atgtgacata cgtgcctgcc caagagaaga atttcacaac
     8641 cgcccctgcc atctgccacg acggcaaggc ccacttccca agagagggcg ttttcgtttc
     8701 caatggcaca cactggttcg tgacacaaag aaacttctac gaaccccaga ttatcaccac
     8761 cgacaacacc ttcgtgagtg gcaattgtga cgtggtcatc ggaatcgtga acaacacagt
     8821 gtacgaccct ctgcaacctg agctggactc ttttaaggaa gagctggaca agtactttaa
     8881 aaaccacacc agccccgatg tggacctggg cgacatcagt ggcattaacg ccagcgtggt
     8941 gaacatccaa aaggaaatcg acagactgaa cgaggtggcc aagaacctga acgagtccct
     9001 gatcgacctg caggagctcg gcaaatacga gcagggatcc ggatacatcc ccgaggcccc
     9061 cagagatggc caggcctacg tgcggaagga cggcgagtgg gtactgctga gcacattcct
     9121 gggcagatcc gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa
     9181 ggatgacgat gacaagagcg cctggtccca cccccagttc gagaagggat ccctggaggt
     9241 gctgttccag ggcccaggcg gcggcggtag tggagggggc ggatctggcg gctcagctgc
     9301 tgtgggccag gacacgcagg aggtcatcgt ggtgccacac tccttgccct ttaaggtggt
     9361 ggtgatctca gccatcctgg ccctggtggt gctcaccatc atctccctta tcatcctcat
     9421 catgctttgg cagaagaagc cacgt
//
