LOCUS       Exported File           7211 bp ds-DNA    circular SYN 09-DEC-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pLBT0050
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   LGBP
  TITLE     Direct Submission
  JOURNAL   Velay et al. MOBIFC LGBP-BIAM
            
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    complement(32..159)
                     /label="RB short"
                     /note="RB short"
                     /note="color: #66cc99; direction: LEFT"
     misc_feature    complement(183..1664)
                     /label="RK2 trfa (no Esp3I)"
                     /note="RK2 trfa"
                     /note="color: #66cc99; direction: LEFT"
     misc_feature    1675..2464
                     /label="pUC ori"
                     /note="pUC ori"
                     /note="color: #66cc99; direction: RIGHT"
     rep_origin      1710
                     /label="ORI"
                     /note="ORI"
                     /note="RNaseH cleavage point"
                     /note="color: #3366cc"
     CDS             complement(2472..3329)
                     /codon_start=1
                     /label="AP r"
                     /note="AP r"
                     /note="color: #ff9966"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(3527..4144)
                     /direction=LEFT
                     /standard_name="oriV"
                     /label="oriV"
                     /note="oriV"
                     /note="shows similarity to GenBank Accession Number M20134"
                     /note="color: #ff9966"
     misc_feature    4174..4321
                     /label="TDNA-LB"
                     /note="TDNA-LB"
                     /note="shows similarity to T-DNA left border: GenBank 
                     Accession Number J01825"
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    4373..4376
                     /label="GGAG"
                     /note="GGAG"
                     /note="color: #a6acb3"
     misc_feature    4377..5804
                     /label="Pro-CaMV35SLong_5U-TMV"
                     /note="Pro-CaMV35SLong_5U-TMV"
                     /note="color: #a6acb3"
     misc_feature    5806..6151
                     /label="CTP SSU"
                     /note="CTP SSU"
                     /note="color: #a6acb3"
     misc_feature    6152..6712
                     /label="HSP21 CDS2ns"
                     /note="HSP21 CDS2ns"
                     /note="color: #a6acb3"
     misc_feature    6713..7007
                     /label="CYFP 3HA"
                     /note="CYFP 3HA"
                     /note="color: #a6acb3"
     misc_feature    7008..7211
                     /label="3U+Ter"
                     /note="3U+Ter"
                     /note="color: #a6acb3"
ORIGIN
        1 cgctttactt gtcttctgca cgaagtggtt taaactatca gtgtttgaca ggatatattg
       61 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg
      121 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt ccccagatca
      181 ggcgctggct gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg
      241 caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca
      301 ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag
      361 gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat
      421 ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc
      481 gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt
      541 gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg
      601 gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt
      661 gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt
      721 gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc
      781 gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg
      841 gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag
      901 ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc
      961 gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg
     1021 cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt
     1081 catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcaag
     1141 acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac
     1201 gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg actggaaggt
     1261 ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa
     1321 ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc
     1381 tccttgcggg attgccccga ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc
     1441 gcctggtgcc ttggtgtcca gataatccac cttatcggca atgaagtcgg tcccgtagac
     1501 cgtctggccg tccttctcgt acttggtatt ccgaatcttg ccctgcacga ataccagcga
     1561 ccccttgccc aaatacttgc cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa
     1621 gaagtcggtg cgctcctgct tgtcgccggc atcgttgcgc cacatctagg atctgccagg
     1681 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1741 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1801 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1861 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1921 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1981 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2041 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2101 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     2161 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2221 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2281 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2341 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2401 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2461 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2521 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2581 tctggcccca gtgctgcaat gataccgcga gaaccacgct caccggctcc agatttatca
     2641 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2701 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2761 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2821 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2881 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2941 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3001 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3061 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3121 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3181 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3241 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3301 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3361 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3421 ataggggttc cgcgcacgaa ttggccagcg ctgccatttt tggggtgagg ccgttcgcgg
     3481 ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga
     3541 gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta
     3601 aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg
     3661 aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat
     3721 gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc
     3781 cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg
     3841 cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag
     3901 gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg
     3961 ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag
     4021 ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc
     4081 gtttctggcg cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc
     4141 ccggtgagcg tcgcaaagga gatcctgatc tgactgatgg gctgcctgta tcgagtggtg
     4201 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg
     4261 gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact
     4321 ggggtggatg cagtgggccc cactctgtga agacaaacta gaattcgagc tcggagggag
     4381 gaattccaat cccacaaaaa tctgagctta acagcacagt tgctcctctc agagcagaat
     4441 cgggtattca acaccctcat atcaactact acgttgtgta taacggtcca catgccggta
     4501 tatacgatga ctggggttgt acaaaggcgg caacaaacgg cgttcccgga gttgcacaca
     4561 agaaatttgc cactattaca gaggcaagag cagcagctga cgcgtacaca acaagtcagc
     4621 aaacagacag gttgaacttc atccccaaag gagaagctca actcaagccc aagagctttg
     4681 ctaaggccct aacaagccca ccaaagcaaa aagcccactg gctcacgcta ggaaccaaaa
     4741 ggcccagcag tgatccagcc ccaaaagaga tctcctttgc cccggagatt acaatggacg
     4801 atttcctcta tctttacgat ctaggaagga agttcgaagg tgaaggtgac gacactatgt
     4861 tcaccactga taatgagaag gttagcctct tcaatttcag aaagaatgct gacccacaga
     4921 tggttagaga ggcctacgca gcaagtctca tcaagacgat ctacccgagt aacaatctcc
     4981 aggagatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaatt
     5041 gcatcaagaa cacagagaaa gacatatttc tcaagatcag aagtactatt ccagtatgga
     5101 cgattcaagg cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaaaaagg
     5161 tagttcctac tgaatctaag gccatgcatg gagtctaaga ttcaaatcga ggatctaaca
     5221 gaactcgccg tcaagactgg cgaacagttc atacagagtc ttttacgact caatgacaag
     5281 aagaaaatct tcgtcaacat ggtggagcac gacactctgg tctactccaa aaatgtcaaa
     5341 gatacagtct cagaagatca aagggctatt gagacttttc aacaaaggat aatttcggga
     5401 aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag
     5461 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggctatcat tcaagatctc
     5521 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
     5581 gaggttccaa ccacgtctac aaagcaagtg gattgatgtg acatctccac tgacgtaagg
     5641 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
     5701 catttggaga ggacacgctc gagtataaga gctcattttt acaacaatta ccaacaacaa
     5761 caaacaacaa acaacattac aattacattt acaattatcg atacaatggc ttcctctatg
     5821 ctctcttccg ctactatggt tgcctctccg gctcaggcca ctatggtcgc tcctttcaac
     5881 ggacttaagt cctccgctgc cttcccagcc acccgcaagg ctaacaacga cattacttcc
     5941 atcacaagca acggcggaag agttaactgc atgcaggtca tttatatttc ttctttcact
     6001 tttttattat tccatatgat ttttttcggt tctttcttcg aatctacata aactaatatc
     6061 attggaaaaa tcgaaaaaat aggtgtggcc tccgattgga aagaagaagt ttgagactct
     6121 ctcttacctt cctgacctta ccgattccga aggtgctcaa gaccagagag aaaactccat
     6181 tgatgttgtt cagcaaggac aacaaaaagg gaaccaagga tcaagtgtcg aaaaaagacc
     6241 tcaacaacgc ttaaccatgg atgtctctcc tttcggattg ttggatcctt tgtcaccaat
     6301 gaggacgatg cgacaaatgt tagatactat ggacaggatg ttcgaggaca ctatgcctgt
     6361 ctcaggaaga aacagaggag gaagtggagt gtcagagatt cgtgcaccgt gggacatcaa
     6421 agaggaagaa cacgagatca agatgcgttt cgacatgcct ggactctcta aagaggacgt
     6481 caaaatctct gtagaagata acgtacttgt gatcaaagga gagcagaaga aggaggacag
     6541 tgatgattct tggtctggaa gaagcgttag ctcatatgga acacgacttc agctcccaga
     6601 caactgtgag aaagacaaga tcaaagctga gctcaagaac ggagtcctct ttatcactat
     6661 ccctaagacc aaagtcgaac gcaaagtcat cgatgtccag attcaatctt cgggcagcgt
     6721 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     6781 cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga
     6841 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     6901 gtacaagtac ccatacgatg ttcctgacta tgcgggctat ccctatgacg tcccggacta
     6961 tgcaggatcc tatccatatg acgttccaga ttacgctgct taagcttctc tagctagagt
     7021 cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga taagggaatt
     7081 agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt
     7141 gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtact
     7201 aaaatccaga t
//
