LOCUS       Exported                9042 bp ds-DNA     circular SYN 20-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSF3-ultraID
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported 20. Jun 2021 from SnapGene 3.0.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             402..995
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1058..1415
                     /note="MMLV Psi"
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     misc_recomb     1933..1966
                     /note="F3"
                     /note="vntifkey=""21"" - label=F3site -;1933..1966"
     polyA_signal    2074..2208
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(2396..4048)
                     /note="Firefly\Luciferase"
                     /note="vntifkey=""4"" - label=FireflyLuciferase 
                     -;complement(2396..4048)"
     promoter        complement(4059..4183)
                     /note="PminCMV-2"
                     /note="vntifkey=""29"" - label=PminCMV-2 
                     -;complement(4059..4183)"
     protein_bind    4201..4471
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        4488..4612
                     /note="PminCMV-1"
                     /note="vntifkey=""29"" - label=PminCMV-1 -;4488..4612"
     misc_feature    4632..4661
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
     CDS             4665..5174
                     /codon_start=1
                     /locus_tag="microID"
                     /label=microID
                     /note="ultraID"
                     /note="microID"
                     /translation="FKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGPGRKWL
                     SQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVS
                     GVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISEN
                     LKKFKEK"
     misc_feature    4779..4781
                     /locus_tag="R40G mutation"
                     /label=R40G mutation
                     /note="R40G mutation"
     misc_feature    4782..4784
                     /note="L41P mutation"
     protein_bind    5280..5327
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     LTR             5474..5711
                     /note="3'\LTR"
                     /note="vntifkey=""19"" - label=3'LTR -;8524..8761"
     primer_bind     complement(6414..6430)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6905..7009
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             7010..7870
                     /note="AmpR"
                     /note="vntifkey=""4"" - label=AmpR -;10060..10920"
     rep_origin      8041..8629
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8917..8938
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8953..8983
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8991..9007
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9015..9031
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caagctagct ttgctcttag gagtttccta atacatccca aactcaaata tataaagcat
       61 ttgacttgtt ctatgcccta gggggcgggg ggaagctaag ccagcttttt ttaacattta
      121 aaatgttaat tccattttaa atgcacagat gtttttattt cataagggtt tcaatgtgca
      181 tgaatgctgc aatattcctg ttaccaaagc tagtataaat aaaaatagat aaacgtggaa
      241 attacttaga gtttctgtca ttaacgtttc cttcctcagt tgacaacata aatgcgctgc
      301 tgagcaagcc agtttgcatc tgtcaggatc aatttcccat tatgccagtc atattaatta
      361 ctagtcaatt agttgatttt tatttttgac atatacatgt gaatgaaaga ccccacctgt
      421 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg
      481 agaatagaaa agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac
      541 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct
      601 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      661 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt
      721 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      781 gcttctcgct tctgttcgcg cgcttatgct ccccgagctc aataaaagag cccacaaccc
      841 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
      901 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
      961 agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga
     1021 cccctgccca gggaccaccg acccaccacc gggaggtaag ctggccagca acttatctgt
     1081 gtctgtccga ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag
     1141 ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttcgga acacccggcc
     1201 gcaaccctgg gagacgtccc agggacttcg ggggccgttt ttgtggcccg acctgagtcc
     1261 taaaatcccg atcgtttagg actctttggt gcacccccct tagaggaggg atatgtggtt
     1321 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     1381 tgggaccgaa gccgcgccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
     1441 ctgactgtgt ttctgtattt gtctgaaaat atgggcccgg gctagcctgt taccactccc
     1501 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
     1561 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
     1621 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
     1681 ttttcacctg gcccgcatgg acacccagac caggtggggt acatcgtgac ctgggaagcc
     1741 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
     1801 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc
     1861 tccctttatc cagccctcac tccttctcta ggcgccccca tatggccata tgagatccga
     1921 agttcctatt ccgaagttcc tattcttcaa atagtatagg aacttcagat cataatcagc
     1981 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     2041 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     2101 tacaaataag gcaatagcat cacaaatttc acaaataagg catttttttc actgcattct
     2161 agttttggtt tgtccaaact catcaatgta tcttatcatg tctggatctc aaatccctcg
     2221 gaagctgcgc ctgtcttagg ttggagtgat acatttttat cacttttacc cgtctttgga
     2281 ttaggcagta gctctgacgg ccctcctgtc ttaggttagt gaaaaatgtc actctcttac
     2341 ccgtcattgg ctgtccagct tagctcgcag gggaggtggt ctggatcctc tagaattaca
     2401 cggcgatctt tccgcccttc ttggccttta tgaggatctc tctgattttt cttgcgtcga
     2461 gttttccggt aagacctttc ggtacttcgt ccacaaacac aactcctccg cgcaactttt
     2521 tcgcggttgt tacttgactg gccacgtaat ccacgatctc tttttccgtc atcgtctttc
     2581 cgtgctccaa aacaacaacg gcggcgggaa gttcaccggc gtcatcgtcg ggaagacctg
     2641 cgacacctgc gtcgaagatg ttggggtgtt ggagcaagat ggattccaat tcagcgggag
     2701 ccacctgata gcctttgtac ttaatcagag acttcaggcg gtcaacgatg aagaagtgtt
     2761 cgtcttcgtc ccagtaagct atgtctccag aatgtagcca tccatccttg tcaatcaagg
     2821 cgttggtcgc ttccggattg tttacataac cggacataat cataggacct ctcacacaca
     2881 gttcgcctct ttgattaacg cccagcgttt tcccggtatc cagatccaca accttcgctt
     2941 caaaaaatgg aacaacttta ccgaccgcgc ccggtttatc atccccctcg ggtgtaatca
     3001 gaatagctga tgtagtctca gtgagcccat atccttgcct gatacctggc agatggaacc
     3061 tcttggcaac cgcttccccg acttccttag agaggggagc gccaccagaa gcaatttcgt
     3121 gtaaattaga taaatcgtat ttgtcaatca gagtgctttt ggcgaagaag gagaataggg
     3181 ttggcaccag cagcgcactt tgaatcttgt aatcctgaag gctcctcaga aacagctctt
     3241 cttcaaatct atacattaag acgactcgaa atccacatat caaatatccg agtgtagtaa
     3301 acattccaaa accgtgatgg aatggaacaa cacttaaaat cgcagtatcc ggaatgattt
     3361 gattgccaaa aataggatct ctggcatgcg agaatctcac gcaggcagtt ctatgaggca
     3421 gagcgacacc tttaggcaga ccagtagatc cagaggagtt catgatcagt gcaattgtct
     3481 tgtccctatc gaaggactct ggcacaaaat cgtattcatt aaaaccggga ggtagatgag
     3541 atgtgacgaa cgtgtacatc gactgaaatc cctggtaatc cgttttagaa tccatgataa
     3601 taattttttg gatgattggg agcttttttt gcacgttcaa aattttttgc aacccctttt
     3661 tggaaacgaa caccacggta ggctgcgaaa tgcccatact gttgagcaat tcacgttcat
     3721 tataaatgtc gttcgcgggc gcaactgcaa ctccgataaa taacgcgccc aacaccggca
     3781 taaagaattg aagagagttt tcactgcata cgacgattct gtgatttgta ttcagcccat
     3841 atcgtttcat agcttctgcc aaccgaacgg acatttcgaa gtactcagcg taagtgatgt
     3901 ccacctcgat atgtgcatct gtaaaagcaa ttgttccagg aaccagggcg tatctcttca
     3961 tagccttatg cagttgctct ccagcggttc catcttccag cggatagaat ggcgccgggc
     4021 ctttctttat gtttttggcg tcttccatgg ttgtcgaccg cggaggctgg atcggtcccg
     4081 gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac
     4141 taaacgagct ctgcttatat aggcctccca ccgtacacgc ctacaagctt ctttcacttt
     4201 tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc actgataggg
     4261 agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt
     4321 cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg
     4381 atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc
     4441 gactttcact tttctctatc actgataggg agtggtaaac tcgagggtag gcgtgtacgg
     4501 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     4561 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggaattcgg
     4621 ccggccccat ggaacaaaaa ctcatctcag aagaggatct cgacttcaag aacctgatct
     4681 ggctgaagga ggtggacagc acccaggaga gactgaagga gtggaacgtg tcctacggca
     4741 ccgccctggt ggccgacaga cagaccaagg gcagaggcgg cccgggcaga aagtggctga
     4801 gccaggaggg cggcctgtac ttcagcttcc tgctgaaccc caaggagttc gagaacctgc
     4861 tgcagctgcc cctggtgctg ggcctgagcg tgagcgaggc cctggaggag atcaccgaga
     4921 tccccttcag cctgaagtgg cccaacgacg tgtacttcca ggagaagaag gtgagcggcg
     4981 tgctgtgcga gctgagcaag gacaagctga tcgtgggcat cggcatcaac gtgaaccaga
     5041 gagagatccc cgaggagatc aaggacagag ccaccaccct gtacgagatc accggcaagg
     5101 actgggacag aaaggaggtg ctgctgaagg tgctgaagag aatcagcgag aacctgaaga
     5161 agttcaagga gaaggggcgg ccgccactgt gctggatatc tgcagaattc caccacactg
     5221 gactagtgga tccgagctcg gtaccaagct taagtttaaa ccgctgaaga tctggccgcg
     5281 aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcaga tccggattag
     5341 tccaatttgt taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc
     5401 accagctgaa gcctatagag tacgagccat agataaaata aaagatttta tttagtctcc
     5461 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct cgacttctgt
     5521 tcgcgcgctt ctgctccccg agctcgagcc cacaacccct cactcggggc gccagtcctc
     5581 cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
     5641 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
     5701 ggggtctttc acacatgcag catgtatcaa aattaatttg gttttttttc ttaagtattt
     5761 acattaaatg gccatagtac ttaaagttac attggcttcc ttgaaataaa catggagtat
     5821 tcagaatgtg tcataaatat ttctaatttt aagatagtat ctccattggc tttctacttt
     5881 ttcttttatt tttttttgtc ctctgtcttc catttgttgt tgttgttgtt tgtttgtttg
     5941 tttgttggtt ggttggttaa ttttttttta aagatcctac actatagttc aagctagact
     6001 attagctact ctgtaaccca gggtgacctt gaagtcatgg gtagcctgct gttttagcct
     6061 tcccacatct aagattacag gtatgagcta tcatttttgg tatattgatt gattgattga
     6121 ttgatgtgtg tgtgtgtgat tgtgtttgtg tgtgtgactg tgaaaatgtg tgtatgggtg
     6181 tgtgtgaatg tgtgtatgta tgtgtgtgtg tgagtgtgtg tgtgtgtgtg tgcatgtgtg
     6241 tgtgtgtgac tgtgtctatg tgtatgactg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     6301 tgtgtgtgtg tgtgttgtga aaaaatattc tatggtagtg agagccaacg ctccggctca
     6361 ggtgtcaggt tggtttttga gacagagtct ttcacttagc ttggaattaa ttcactggcc
     6421 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6481 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6541 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
     6601 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     6661 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     6721 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     6781 ttttcaccgt catcaccgaa acgcgcgatg acgaaagggc ctcgtgatac gcctattttt
     6841 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     6901 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6961 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7021 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7081 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7141 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7201 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7261 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7321 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     7381 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7441 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7501 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7561 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7621 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7681 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7741 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7801 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7861 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7921 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7981 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8041 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8101 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8161 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     8221 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8281 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8341 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8401 ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg
     8461 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8521 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8581 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8641 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8701 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8761 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     8821 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     8881 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     8941 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     9001 ataacaattt cacacaggaa acagctatga ccatgattac gc
//
