LOCUS       pZa_native_TetO_bxb1_pb 6833 bp ds-DNA     circular     21-MAR-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(717..1376)
                     /label="CmR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1509..1619
                     /label="Cloned bölge"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1620..2466
                     /label="cloned region"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(1752..2378)
                     /label="tetR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2430..2448
                     /label="TetO"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     RBS             2468..2473
                     /label="RBS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2479..2479
                     /label="5'GibSeq (concatenated sequence 1)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2479..2479
                     /label="Extracted region from null"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          2480..2497
                     /label="pREA120"
                     /note="sequence: aagaattcaaaaGATCTGAAGGAGGGTACCATGGGAACGGTGGCGCAG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             2480..3607
                     /label="Bxb1 integrase"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             2480..3607
                     /label="Translation 2480-3607"
     misc_feature    3629..4981
                     /label="Cloned bölge"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(3746..4624)
                     /label="AraC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        4897..4925
                     /label="ARA promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             4982..6508
                     /label="TP901 integrase"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(6485..6508)
                     /label="pREA121"
                     /note="sequence: GAGCCTTTCGTTTTATTTGATGCCACGCGTTTAAGCAGCCAGAGCGTAGTTTTC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    6510..6513
                     /label="Ligation"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      6515..6619
                     /label="rrnB T1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    join(6626..6833,1..590)
                     /label="p15A origin"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGCGG CTCCCTCGTG
       61 CGCTCTCCTG TTCCTGCCTT TCGGTTTACC GGTGTCATTC CGCTGTTATG GCCGCGTTTG
      121 TCTCATTCCA CGCCTGACAC TCAGTTCCGG GTAGGCAGTT CGCTCCAAGC TGGACTGTAT
      181 GCACGAACCC CCCGTTCAGT CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
      241 CAACCCGGAA AGACATGCAA AAGCACCACT GGCAGCAGCC ACTGGTAATT GATTTAGAGG
      301 AGTTAGTCTT GAAGTCATGC GCCGGTTAAG GCTAAACTGA AAGGACAAGT TTTGGTGACT
      361 GCGCTCCTCC AAGCCAGTTA CCTCGGTTCA AAGAGTTGGT AGCTCAGAGA ACCTTCGAAA
      421 AACCGCCCTG CAAGGCGGTT TTTTCGTTTT CAGAGCAAGA GATTACGCGC AGACCAAAAC
      481 GATCTCAAGA AGATCATCTT ATTAATCAGA TAAAATATTT CTAGATTTCA GTGCAATTTA
      541 TCTCTTCAAA TGTAGCACCT GAAGTCAGCC CCATACGATA TAAGTTGTTA CTAGTGCTTG
      601 GATTCTCACC AATAAAAAAC GCCCGGCGGC AACCGAGCGT TCTGAACAAA TCCAGATGGA
      661 GTTCTGAGGT CATTACTGGA TCTATCAACA GGAGTCCAAG CGAGCTCGAT ATCAAATTAC
      721 GCCCCGCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG
      781 AAGCCATCAC AGACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT
      841 TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG
      901 TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA
      961 ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT
     1021 ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA
     1081 GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG
     1141 TCTTTCATTG CCATACGGAA TTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA
     1201 AAGGCCGGAT AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC
     1261 AGCTGAACGG TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT
     1321 TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCATTTTA
     1381 GCTTCCTTAG CTCCTGAAAA TCTCGATAAC TCAAAAAATA CGCCCGGTAG TGATCTTATT
     1441 TCATTATGGT GAAAGTTGGA ACCTCTTACG TGCCGATCAA CGTCTCATTT TCGCCAGATA
     1501 TCGACGTCtc acactggctc accttcgggt gggcctttct gcgtttatat actagagaga
     1561 gaatataaaa agccagatta ttaatccggc ttttttatta tttCCTGCAG Ggcggccgct
     1621 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1681 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccag
     1741 atatcgacgt cttaagaccc actttcacat ttaagttgtt tttctaatcc gcatatgatc
     1801 aattcaaggc cgaataagaa ggctggctct gcaccttggt gatcaaataa ttcgatagct
     1861 tgtcgtaata atggcggcat actatcagta gtaggtgttt ccctttcttc tttagcgact
     1921 tgatgctctt gatcttccaa tacgcaacct aaagtaaaat gccccacagc gctgagtgca
     1981 tataatgcat tctctagtga aaaaccttgt tggcataaaa aggctaattg attttcgaga
     2041 gtttcatact gtttttctgt aggccgtgta cctaaatgta cttttgctcc atcgcgatga
     2101 cttagtaaag cacatctaaa acttttagcg ttattacgta aaaaatcttg ccagctttcc
     2161 ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca tctcaatggc taaggcgtcg
     2221 agcaaagccc gcttattttt tacatgccaa tacaatgtag gctgctctac acctagcttc
     2281 tgggcgagtt tacgggttgt taaaccttcg attccgacct cattaagcag ctctaatgcg
     2341 ctgttaatca ctttactttt atctaatcta gacatcatta attcctaatt tttgttgaca
     2401 ctctatcgtt gatagagtta ttttaccact ccctatcagt gatagagaaa agaattcaaa
     2461 aGATCTGAAG GAGGGTACCA TGGGAACGGT GGCGCAGATG GAATTAGAAG CGATCAAAGA
     2521 GCGGAACCGT TCGGCTGCGC ATTTCAATAT CCGCGCCGGG AAATACCGAG GATCCCTGCC
     2581 GCCGTGGGGA TACCTGCCTA CGCGCGTGGA CGGGGAGTGG CGGCTGGTGC CGGACCCTGT
     2641 GCAGCGAGAG CGCATCCTCG AGGTGTATCA CCGCGTCGTC GACAACCACG AGCCGCTGCA
     2701 TCTGGTGGCC CACGACCTGA ACCGGCGTGG TGTCCTGTCG CCGAAGGACT ACTTCGCGCA
     2761 GCTGCAAGGC CGCGAGCCGC AGGGCCGGGA GTGGTCGGCT ACCGCGCTGA AGCGATCGAT
     2821 GATCTCCGAG GCGATGCTCG GGTACGCGAC TCTGAACGGT AAGACCGTCC GAGACGACGA
     2881 CGGAGCCCCG CTGGTGCGGG CTGAGCCGAT CCTGACCCGT GAGCAGCTGG AGGCGCTGCG
     2941 CGCCGAGCTC GTGAAGACCT CCCGGGCGAA GCCCGCGGTG TCTACCCCGT CGCTGCTGCT
     3001 GCGGGTGTTG TTCTGCGCGG TGTGCGGGGA GCCCGCGTAC AAGTTCGCCG GGGGAGGACG
     3061 TAAGCACCCG CGCTACCGCT GCCGCTCGAT GGGGTTCCCG AAGCACTGCG GGAACGGCAC
     3121 GGTGGCGATG GCCGAGTGGG ACGCGTTCTG CGAGGAGCAG GTACTGGATC TGCTCGGGGA
     3181 CGCGGAGCGT CTGGAGAAAG TCTGGGTAGC GGGCTCGGAC TCCGCGGTCG AACTCGCGGA
     3241 GGTGAACGCG GAGCTGGTGG ACCTGACGTC GCTGATCGGC TCCCCGGCCT ACCGGGCGGG
     3301 CTCTCCGCAG CGAGAAGCAC TGGATGCCCG TATTGCGGCG CTGGCCGCGC GGCAAGAGGA
     3361 GCTGGAGGGC CTGGAGGCTC GCCCGTCTGG CTGGGAGTGG CGCGAGACCG GGCAGCGGTT
     3421 CGGGGACTGG TGGCGGGAGC AGGACACCGC GGCAAAGAAC ACCTGGCTTC GGTCGATGAA
     3481 CGTTCGGCTG ACGTTCGACG TCCGCGGCGG GCTGACTCGC ACGATCGACT TCGGGGATCT
     3541 TCAGGAGTAC GAGCAGCATC TCAGGCTCGG CAGCGTGGTC GAACGGCTAC ACACCGGGAT
     3601 GTCGtaaTAA TACTAGATGC ATTAATAAtc acactggctc accttcgggt gggcctttct
     3661 gcgtttatat actagagaga gaatataaaa agccagatta ttaatccggc ttttttatta
     3721 tttCCTGCAG GgcggccgcC ATATGttatg acaacttgac ggctacatca ttcacttttt
     3781 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
     3841 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
     3901 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
     3961 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
     4021 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
     4081 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
     4141 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
     4201 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
     4261 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
     4321 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
     4381 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
     4441 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
     4501 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
     4561 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
     4621 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
     4681 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta
     4741 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
     4801 tctataatca cggcagaaaa gtcacattga ttatttgcac ggcgtcacac tttgctatgc
     4861 catagcattt ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc
     4921 tactgtttct ccataccgtt tttttgggct agctactaga gtcacacagg aaacctacta
     4981 gATGAAACAT CATCACCATC ACCACCAGGC CGGCactaag aaagtagcaa tctatacacg
     5041 agtatccact actaaccaag cagaggaagg cttctcaatt gatgagcaaa ttgaccgttt
     5101 aacaaaatat gctgaagcaa tggggtggca agtatctgat acttatactg atgctggttt
     5161 ttcaggggcc aaacttgaac gcccagcaat gcaaagatta atcaacgata tcgagaataa
     5221 agcttttgat acagttcttg tatataagct agaccgcctt tcacgtagtg taagagatac
     5281 tctttatctt gttaaggatg tgttcacaaa aaataaaata gactttatct cgcttaatga
     5341 aagtattgat acttcttctg ctatgggtag cttgtttctc actattcttt ctgcaattaa
     5401 tgagtttgaa agagagaata taaaagaacg catgactatg ggtaaactag ggcgagcgaa
     5461 atctggtaag tctatgatgt ggactaagac agcttttggg tattaccaca acagaaagac
     5521 aggtatatta gaaattgttc ctttacaagc tacaatagtt gaacaaatat tcactgatta
     5581 tttatcagga atatcactta caaaattaag agataaactc aatgaatctg gacacatcgg
     5641 taaagatata ccgtggtctt atcgtaccct aagacaaaca cttgataatc cagtttactg
     5701 tggttatatc aaatttaagg acagcctatt tgaaggtatg cacaaaccaa ttatccctta
     5761 tgagacttat ttaaaagttc aaaaagagct agaagaaaga caacagcaga cttatgaaag
     5821 aaataacaac cctagacctt tccaagctaa atatatgctg tcagggatgg caaggtgcgg
     5881 ttactgtgga gcacctttaa aaattgttct tggccacaaa agaaaagatg gaagccgcac
     5941 tatgaaatat cactgtgcaa atagatttcc tcgaaaaaca aaaggaatta cagtatataa
     6001 tgacaataaa aagtgtgatt caggaactta tgatttaagt aatttagaaa atactgttat
     6061 tgacaacctg attggatttc aagaaaataa tgactcctta ttgaaaatta tcaatggcaa
     6121 caaccaacct attcttgata cttcgtcatt taaaaagcaa atttcacaga tcgataaaaa
     6181 aatacaaaag aactctgatt tgtacctaaa tgattttatc actatggatg agttgaaaga
     6241 tcgtactgat tcccttcagg ctgagaaaaa gctgcttaaa gctaagatta gcgaaaataa
     6301 atttaatgac tctactgatg tttttgagtt agttaaaact cagttgggct caattccgat
     6361 taatgaacta tcatatgata ataaaaagaa aatcgtcaac aaccttgtat caaaggttga
     6421 tgttactgct gataatgtag atatcatatt taaattcCAA CTCGCTACCG GTGCTGCTAA
     6481 GGACGAAAAC TACGCTCTGG CTGCTTAAAC GCGTGGCATC AAATAAAACG AAAGGCTCAG
     6541 TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACGCTCT CCTGAGTAGG
     6601 ACAAATCCGC CGCCCTAGAC CTAGGGGATA TATTCCGCTT CCTCGCTCAC TGACTCGCTA
     6661 CGCTCGGTCG TTCGACTGCG GCGAGCGGAA ATGGCTTACG AACGGGGCGG AGATTTCCTG
     6721 GAAGATGCCA GGAAGATACT TAACAGGGAA GTGAGAGGGC CGCGGCAAAG CCGTTTTTCC
     6781 ATAGGCTCCG CCCCCCTGAC AAGCATCACG AAATCTGACG CTCAAATCAG TGG
//