LOCUS       Exported               10515 bp DNA     circular SYN 14-JUN-2017
DEFINITION  Cloning vector pUC19, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10515)
  AUTHORS   New England Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs, 
            240 County Road, Ipswich, MA 01938, USA
REFERENCE   2  (bases 1 to 10515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 21, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (18-OCT-2007) Research Department, New England Biolabs, 240 County 
            Road, Ipswich, MA 01938, USA"
COMMENT     To prepare vector for cloning:
            -Digest with ClaI and SpeI to fuse 5' homology arm directly to 
            mKate2, or
            -Digest with AvrII and SpeI to include a flexible linker (for 
            C-terminal tags).
FEATURES             Location/Qualifiers
     source          1..10515
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     378..395
                     /label=M13-fwd
     misc_feature    398..1010
                     /label=This sequence will be replaced by the homology arm
     misc_feature    661..966
                     /label=ccdB
                     /note="/translation=MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQL
                     DITAF 
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF 
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV 
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG 
                     A*"
     CDS             754..963
                     /label=ccdB
                     /label=ccdB(1)
     misc_structure  1011..1037
                     /label=Flexible Linker (will be deleted if ClaI is used)
     CDS             join(1038..1241,1293..1451,1503..1667,1719..1886)
                     /label=mKate2
     misc_signal     1887..1907
                     /label=TEV site
     intron          1914..1924
                     /label=intron(1)
     misc_feature    1925..1958
                     /label=loxP
     intron          1959..1964
                     /label=intron
     misc_feature    1965..1967
                     /label=spacer
     misc_feature    1968..1997
                     /label=myc
     terminator      2001..2381
                     /label=let-858 Terminator
     promoter        2382..2751
                     /label=Psqt1
     5'UTR           join(2666..2693,2750..2751)
                     /label=sqt-1 5'UTR
     CDS             join(2752..2892,2940..3773)
                     /label=SQT-1
     misc_difference 3003
                     /label=e1350
     3'UTR           3774..3810
                     /label=sqt-1 3'UTR
     3'UTR           complement(3811..4142)
                     /label=tbb-2 3'UTR
     CDS             complement(join(4146..4387,4439..4809,4861..5276))
                     /label=Cre
     promoter        complement(5277..5676)
                     /label=hsp16-41 promoter
     promoter        5677..6039
                     /label=Prps-0
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink; 
                     /ApEinfo_revcolor=pink; /ApEinfo_graphicformat=arrow_data 
                     {{0 1 2 0 0 -1} {} 0} width 5 offset 0"
     CDS             6082..7107
                     /label=hygR
                     /note="hygromycinR"
     3'UTR           7133..7484
                     /label=unc-54 3'UTR
     misc_feature    7485..7518
                     /label=loxP
                     /label=loxP(1)
     intron          7519..7524
                     /label=intron(2)
     misc_feature    7525..7536
                     /label=spacer
                     /label=spacer(1)
     misc_feature    7537..7671
                     /label=Degron
     misc_feature    7671
                     /label=AAG --> AAA mut for SpeI site
     misc_feature    7672..8278
                     /label=This sequence will be replaced by the
                     homology arm
                     /label=This sequence will be replaced by the homology
                     arm(1)
     misc_feature    complement(7716..8021)
                     /label=ccdB
                     /label=ccdB(2)
                     /note="/translation=MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQL
                     DITAF 
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF 
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV 
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG 
                     A*"
     CDS             complement(7719..7928)
                     /label=ccdB
                     /label=ccdB(3)
     misc_feature    8279..8285
                     /label=This sequence will be deleted if ClaI is used
     primer_bind     complement(8290..8310)
                     /label=M13-rev
     rep_origin      complement(8678..9360)
                     /direction=LEFT
                     /label=ColE1 origin
     rep_origin      complement(8696..9284)
                     /direction=LEFT
                     /label=pMB1 origin of replication (counter-clockwise) (RN
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
     misc_RNA        complement(8696..9248)
                     /label=RNAII transcript (complementary strand)
                     /note="RNAII transcript (complementary strand)"
     -35_signal      9102..9107
                     /label=RNAI promoter (clockwise)
                     /label=RNAI promoter (clockwise); TTGAAG
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      9124..9129
                     /label=RNAI promoter (clockwise)
                     /label=RNAI promoter (clockwise); GCTACA
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        9138..9245
                     /label=RNAI transcript
                     /note="RNAI transcript"
     -10_signal      complement(9258..9263)
                     /label=RNAII promoter (counter-clockwise)
                     /label=RNAII promoter (counter-clockwise); CGTAAT
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
     -35_signal      complement(9279..9284)
                     /label=RNAII promoter (counter-clockwise)
                     /label=RNAII promoter (counter-clockwise); TTGAGA
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
     CDS             complement(9455..10315)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampR (confers resistance to ampicillin)"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(9458..10117)
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtta cccgaagtat
      421 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
      481 cagttgctca aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga
      541 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
      601 aggtcgcccg gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa
      661 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
      721 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      781 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      841 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
      901 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      961 atataaatgt caggctccct tatacacagc cagtctgcag gtcgacctag ggagcatcgg
     1021 gagcctcagg agcatcgatg gtctccgagc tcattaaaga aaacatgcat atgaagctct
     1081 acatggaggg aaccgtcaac aaccaccact tcaagtgcac ctccgaggga gagggaaagc
     1141 catacgaggg aacccaaacc atgcgtatca aggccgtcga gggaggacca ctcccattcg
     1201 ccttcgacat cctcgccacc tccttcatgt acggatccaa ggtaagttta aacatatata
     1261 tactaactaa ccctgattat ttaaattttc agaccttcat caaccacacc caaggaatcc
     1321 cagacttctt caagcaatcc ttcccagagg gattcacctg ggagcgtgtc accacctacg
     1381 aggacggagg agtcctcacc gccacccaag acacctccct ccaagacgga tgcctcatct
     1441 acaacgtcaa ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
     1501 agatccgtgg agtcaacttc ccatccaacg gaccagtcat gcaaaagaag accctcggat
     1561 gggaggcctc caccgagacc ctctacccag ccgacggagg acttgaggga cgtgccgaca
     1621 tggccctcaa gctcgtcgga ggaggacacc tcatctgcaa cctcaaggta agtttaaaca
     1681 tgattttact aactaactaa tctgatttaa attttcagac cacctaccgt tccaagaagc
     1741 cagccaagaa cctcaagatg ccaggagtct actacgtcga ccgtcgtctt gagcgtatca
     1801 aggaggccga caaggagacc tacgtcgagc aacacgaggt cgccgtcgcc cgttactgcg
     1861 acctcccatc caagctcgga caccgtgaga atctgtactt tcaatccgga aaggtaagtt
     1921 taaaataact tcgtatagca tacattatac gaagttattt tcagggagag caaaagctta
     1981 tctccgagga ggacctctaa ggatgatcga cgccaacgtc gttgaatttt caaattttaa
     2041 atactgaata tttgtttttt ttcctattat ttatttattc tctttgtgtt ttttttcttg
     2101 ctttctaaaa aattaattca atccaaatct aaacattttt ttttctcttt ccgtctccca
     2161 attcgtattc cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt
     2221 catttcatta ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga
     2281 aatgaaataa ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt
     2341 aaaagaaaac ataaaaatat atagaggagg aaggaaacta gctgccctcg attgtctatc
     2401 ttccattctc accaagtttc aacaacttta ttcagtcatt cttctctggt tcatttccca
     2461 accaatcttc ccgaataaag cccatctgtc ctttcggcca cattcctcac ctacatccgt
     2521 actccttatc tcccgttctt atccagtctt ttatcgtctc tgcgtctcgc acactgtctc
     2581 gtcggtaatg taaaaaaaaa acaaaaaaaa cttgaaaact tgcggaaaga tttcctaata
     2641 aaaggagacg aatccggtag gaaatcgaca agtctctgct tctcgaagac taggtgagtt
     2701 acatttcttt ggccgaatta ttttgaaatc ctaaatttga taatttcaga gatgtctgta
     2761 aaacttgcgt gttatgtgac ggcttcggtc actgtcgcca ctcttatggt atgcttcatg
     2821 accatgtcaa ccatctactc ggaggttgat ggattcagag agaagcttga taccgagatg
     2881 aatgtgttca gagtgagtta aattatattt tgaattttaa ataattttta attttctagc
     2941 aatctaccaa tggattgtgg aaggacatag ttgtcatcgg aagatctagc aagcgtgtcc
     3001 gttgtcaata tgaagagacc aacgctaccc caactccaca tgctgatgga tccccatctg
     3061 ctccaccagg tcaaccacca gcagttccac cagtcttcaa ccagccaaag actccaaatg
     3121 gagccaatgg aaatggacca acctgcaact gcaatgctga taacaagtgc ccagctggac
     3181 catccggacc aaagggagtt ccaggagttc caggactcga cggagttcca ggacttgacg
     3241 gtgttccagg agttggagct gatgatatcg ctccacaacg cgagtctgtc ggatgcttca
     3301 cttgcccaca aggaccagtt ggaccaccag gagctcttgg aagaccagga ccacgtggac
     3361 ttccaggacc aagaggacaa aatggaaacc caggaagaga tggacaacca ggacatccag
     3421 gagagcaagg atcatccggt caaatcggaa agatcggaga gccaggacca ccaggagaga
     3481 agggacgcga cgccgagcat ccaatcggaa gaccaggacc aaagggacca agaggagatc
     3541 aaggaccaac aggaccagct ggacagaacg gtcttcacgg accaccagga gagccaggaa
     3601 ccgttggacc agaaggacca tctggaaagc aaggacgtca aggaccagac ggaacccagg
     3661 gagagactgg accagacgga agaccaggaa aggatgccga gtactgccag tgcccagaca
     3721 agtctccacc atcagaggct gtcaacgcca accgtggata cagaaatatc taaattgttg
     3781 gtgttttcta ataaaatatt tgagattcag tgagactttt ttcttggcgg cacaataaag
     3841 tttcctcttt ttgttgggtc actcaacgaa cacaatacaa atatttgagc gctcgcattt
     3901 ttctcaacct ttcgggttta aagcacttca aaatggaatt gaagagagat tgtgaaagta
     3961 catcagacag aatcacattt tgatagcatt cacttcactc agatgcaaac ttttcagtga
     4021 ctataaaaag tcataaaata ataacattaa aaaagcaaat aaattagcga gagaattttt
     4081 tgacaaaaag aaagaagagt gatagagaag aagggaatgc ttgaaaggat tttgcattta
     4141 tcttaatcac cgtcttcgag aagacgaacc atggcgccag tttcagaatc caagtttcta
     4201 atatagttca tcacaatgtt gacgttggtc caacctccgg cttgcataat ctctgggatg
     4261 gaaactccag cgcgagccat gtcacgggca gctccgacac gggcggaatg tccagaccaa
     4321 gccaagtaac gttgtccgga atcatccttg gctccgtaga tgagacggtg ggtagcttcg
     4381 aagattcctg aaaatttatt ttcagcttat tcttagctaa tatgttgttt aaacttacct
     4441 tcaagagctc gagtcgagag ttgtgatgtt gcactcgggg cagcaactcc gttcttgcga
     4501 acacgacaga acaggtagtt gtttggatca tcggcgactc cggaaaccga aatccagcgc
     4561 tcaacgagct tggtaactcc aagggagagc gctttttcga ctccggcagt cgagaccagc
     4621 gtttttgtac gtccgatgtg gatgagcatt cttcctccgt ctgtgcgact aatatccttg
     4681 acgcgaatgc gggcaatttc ggcgatacgg agaagtgtgt tgtaagcaat tccgaggaag
     4741 gcgaggtttc ggatgtcctg gcaacgatcg gaattttcca taagagaacg gacctgatcg
     4801 aagtcggttc tgaaaatttc gtcaagtctt aggattgaat acattaatat ttaaacttac
     4861 ctttcgaagg cgagggcttg cttggctctc tctccagcat caacattttc cttgcggata
     4921 cgtctcatta cgagagatac ggcattagaa tctgatgggc gtggaagtcc agatcggcga
     4981 tggagcatat tgagctgtcc gagatgttgt tggatcgtct tcaccgccaa tccacgagct
     5041 tggaggtaga gcaagtagtc acgaacgtct tctggctcgg ctgggaacca cttgcggttg
     5101 ttaagcttgc accaggcagc ccaagaacgg cacactgaga gaagcatctt ccaggtgtgc
     5161 tcggagaagg cttgtctgtc acggaacata tccatgaggt tcttgcgaac ttcatcactt
     5221 gtcgcgtcaa ctggaagtgc tggaagattt tgatgaacag tgagaagatt tgacatattt
     5281 tcgaagtttt ttagatgcac tagaacaaag cgtgttggct tcctctgagc ccgctttcct
     5341 tatatacccg cattctgcag ccttacagaa tgttctagaa ggtcctagat gcattcgttt
     5401 gaaaatactc ccggtgggtg caaagagacg cagacggaaa atgtatctgg gtctctttat
     5461 tgtgtacact acttttccat gtaccgaatg tgagtcgccc tccttttgca acaagcagct
     5521 cgaatgttct agaaaaaggt ggaaaatagt ataaataccg ttgaaaataa ataccgaaca
     5581 acatttgctc taattgtgaa attagaaatc ttcaaactat aatcatgtca ctttaccact
     5641 atttccgtcc agctcaacgt tccgtttttg gtgatcattt ttgctttcgt cgtaaatcta
     5701 cacacgcgtc tcttccgtgc gagagtccaa gccagcagcc aaattcgttg actgagtatt
     5761 caacgtttat acgttgtcgg caacgagaaa taggaaaatg catcgggaaa tgttcttttt
     5821 tcgatttttt ccaaggtttt gacaaatttt accacgaatt ttgctatgtt ttcaattaaa
     5881 aaatatgtta ttcaactgtt tctatgagga aaataaggct ttgcatgtaa ttttcttatt
     5941 cagcataatt tttaattaat ttgaattttc tgtcctaacg tttattttgt tttcttggtt
     6001 atgactgatc tgaaattaat ttttgaattt taaggtaata tgtcaggcgg tgccgcaagt
     6061 ttgtacaaaa aagcaggctc catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     6121 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     6181 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     6241 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     6301 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
     6361 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
     6421 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
     6481 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     6541 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     6601 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     6661 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     6721 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     6781 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     6841 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
     6901 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     6961 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     7021 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     7081 agcactcgtc cgagggcaaa ggaatagacc cagctttctt gtacaaagtg ggtccaatta
     7141 ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta
     7201 tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc tctaacaatg
     7261 aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct
     7321 ccctcccccc attaataata attctatccc aaaatctaca caatgttctg tgtacacttc
     7381 ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa
     7441 taccttatca tatgttacgt ttcagtttat gaccgcaatt tttaataact tcgtatagca
     7501 tacattatac gaagttattt tcagggagcc ggatctatgc ctaaagatcc agccaaacct
     7561 ccggccaagg cacaagttgt gggatggcca ccggtgagat cataccggaa gaacgtgatg
     7621 gtttcctgcc aaaaatcaag cggtggcccg gaggcggcgg cgttcgtgaa actagttcga
     7681 cctgcagact ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta
     7741 atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg
     7801 gagaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc cccgatatgc
     7861 accaccgggt aaagttcacg ggggacttta tctgacagca gacgtgcact ggccaggggg
     7921 atcaccatcc gtcgcccggg cgtgtcaata atatcactct gtacatccac aaacagacga
     7981 taacggctct ctcttttata ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc
     8041 gtcggcaaaa gagccgttca tttcaataaa ccgggcgacc tcagccatcc cttcctgatt
     8101 ttccgctttc cagcgttcgg cacgcagacg acgggcttca ttctgcatgg ttgtgcttac
     8161 cgaaccggag atattgacat catatatgcc ttgagcaact gatagctgtc gctgtcaact
     8221 gtcactgtaa tacgctgctt catagcatac ctctttttga catacttcgg gttcctagga
     8281 aatcgataac atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8341 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8401 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8461 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8521 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8581 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8641 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8701 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8761 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8821 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8881 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8941 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9001 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9061 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9121 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9181 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9241 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9301 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9361 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9421 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9481 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9541 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9601 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9661 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9721 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9781 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9841 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9901 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9961 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10021 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    10081 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10141 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10201 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10261 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10321 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10381 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10441 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
    10501 cgaggccctt tcgtc
//
