LOCUS       Exported                7024 bp DNA     circular SYN 02-SEP-2003
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 21, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7024
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        232..819
                     /label=CMV promoter
                     /label=CMV\promoter
                     /note="/invitrogen=2180000"
                     /note="/vntifkey=29"
     primer_bind     769..789
                     /label=CMV forward primer
     primer_bind     863..882
                     /label=T7 primer
     promoter        863..879
                     /label=T7 promoter
                     /label=T7\promoter
                     /note="/invitrogen=2370000"
                     /note="/vntifkey=30"
     CDS             896..2591
                     /codon_start=1
                     /label=Insert
                     /note="CI:V1=NheI,V2=XbaI,I1=NheI,I2=XbaI"
                     /translation="LAWCPRARNCSPVSCPSWWNWTAT*TATSSPCLARAKATPHTAS*
                     P*SSSAPPESCPCPGLPS*QHCLGACSASPDTPTI*SSTTSSRAPCPRATCRNGPSSLR
                     TTATTRPGPK*SSRATPS*TGSS*RASTSKRTATSWATSWSTTPSTATCTSPPTSRRTA
                     SRPTSA*TATSRTAPCSWPTTTSRTPPSEMAPCCCPTTTT*APRAS*ARTPTRSGTTWC
                     CWNS*PPLASPWAWTSCIRPLPPLEPLLQELLQLEQQQPGPQLLGLPLPELEAEDLAAL
                     AEVADQGDQALMPLQLMLQPQMQPPLTQQLPMLPQPTLPLLLPWCLKGKNCLQAWCLFW
                     WNWMGM*MGTNSACPGRGKGMLPTEN*H*SLSVQQANCLCLGPHS*PPLATA*CVSPAT
                     LII*SSMIFSNPLCLRDMCRKEQFSSRMMGITKHALK*NLRGILS*IALN*KGLISKRM
                     GIFWGTNWSITTTATMCISWLISRKMGSK*TSRSGTISRMEVCSWLIIISRILLSATDL
                     CCCLIITICPTRAPCPRILMRNAIIWCCWNL*QLPELHWGWMNCTSNL"
     primer_bind     complement(2618..2635)
                     /label=BGH reverse primer
     polyA_site      2624..2848
                     /label=BGH pA
                     /label=BGH\pA
                     /note="/invitrogen=1870000"
                     /note="/vntifkey=26"
     rep_origin      2894..3322
                     /label=f1 origin
                     /label=f1\origin
                     /note="/invitrogen=2500000"
                     /note="/vntifkey=33"
     promoter        3327..3696
                     /label=SV40 early promoter
                     /label=SV40\early\promoter
                     /note="/invitrogen=2290000"
                     /note="/vntifkey=29"
     CDS             3732..4526
                     /codon_start=1
                     /label=Neo(R)
                     /note="/invitrogen=1260000"
                     /note="/vntifkey=4"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4700..4830
                     /label=SV40 pA
                     /label=SV40\pA
                     /note="/invitrogen=1840000"
                     /note="/vntifkey=25"
     rep_origin      complement(5213..5883)
                     /direction=LEFT
                     /label=pUC origin
                     /label=pUC\origin
                     /note="/invitrogen=2540000"
                     /note="/vntifkey=33"
     CDS             complement(6028..6888)
                     /codon_start=1
                     /label=Amp(R)
                     /note="/invitrogen=1020000"
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6889..6987)
                     /label=bla promoter
                     /label=bla\promoter
                     /note="/invitrogen=2330000"
                     /note="/vntifkey=30"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 atggtgtcca agggcgagga actgttcacc ggtgtcgtgc ccatcctggt ggaactggac
      961 ggcgacgtga acggccacaa gttctccgtg tctggcgagg gcgaaggcga cgccacatac
     1021 ggcaagctga ccctgaagtt catctgcacc accggaaagc tgcccgtgcc ctggcctacc
     1081 ctcgtgacaa cactgtcttg gggcgtgcag tgcttcgcca gataccccga ccatatgaag
     1141 cagcacgact tcttcaagag cgccatgccc gagggctacg tgcaggaacg gaccatcttc
     1201 tttaaggacg acggcaacta caagaccagg gccgaagtga agttcgaggg cgacaccctc
     1261 gtgaaccgga tcgagctgaa gggcatcgac ttcaaagagg acggcaacat cctgggccac
     1321 aagctggagt acaacgccat ccacggcaac gtgtacatca ccgccgacaa gcagaagaac
     1381 ggcatcaagg ccaacttcgg cctgaactgc aacatcgagg acggctccgt gcagctggcc
     1441 gaccactacc agcagaacac ccccatcgga gatggccccg tgctgctgcc cgacaaccac
     1501 tacctgagca cccagagcaa gctgagcaag gaccccaacg agaagcggga ccacatggtg
     1561 ctgctggaat tcgtgaccgc cgctggcatc accctgggca tggacgagct gtataaggcc
     1621 gctgccgccg ctagagccgc tgctgcaaga gctgctgcag ctagagcagc agcagccagg
     1681 gccgcagctg ctagggctgc cgctgccaga gctggaagcg gaggatctgg cggctctggc
     1741 ggaagtggcg gatcaggggg atcaggcgct gatgccgctg cagctgatgc tgcagccgca
     1801 gatgcagccg ccgctgacgc agcagctgcc gatgctgccg cagccgacgc tgccgctgct
     1861 gcttccatgg tgtctaaagg ggaagaactg tttacaggcg tggtgcctat tctggtggaa
     1921 ctggatgggg atgtgaatgg gcacaaattc agcgtgtccg gggaggggga aggggatgct
     1981 acctacggaa aactgacact gaagtttatc tgtacaacag gcaaactgcc tgtgccttgg
     2041 cccacactcg tgaccacctt tggctacggc ctgatgtgtt tcgcccgcta ccctgatcat
     2101 atgaagcagc atgatttttt caaatccgct atgcctgagg gatatgtgca ggaaagaaca
     2161 attttcttca aggatgatgg gaattacaaa acacgcgctg aagtgaaatt tgagggggat
     2221 actctcgtga atcgcattga actgaaaggg attgatttca aagaggatgg gaatattctg
     2281 gggcacaaac tggagtataa ctacaacagc cacaatgtgt atatcatggc tgataagcag
     2341 aaaaatggga tcaaagtgaa cttcaagatc cggcacaata tcgaggatgg aagtgtgcag
     2401 ctggctgatc attatcagca gaatactcct atcggcgacg gacctgtgct gctgcctgat
     2461 aatcactatc tgtcctacca gagcgccctg tccaaggatc ctaatgagaa acgcgatcat
     2521 atggtgctgc tggaatttgt gacagctgcc ggaattacac tggggatgga tgaactgtac
     2581 aagtaatcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     2641 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     2701 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     2761 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     2821 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     2881 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     2941 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3001 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3061 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     3121 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3181 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     3241 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     3301 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3361 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3421 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3481 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3541 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3601 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3661 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
     3721 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     3781 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     3841 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     3901 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     3961 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     4021 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     4081 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     4141 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     4201 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     4261 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     4321 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     4381 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     4441 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     4501 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     4561 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     4621 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     4681 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     4741 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     4801 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     4861 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4921 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4981 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     5041 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5101 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5161 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5221 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5281 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5341 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5401 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5461 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5521 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5581 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5641 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5701 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5761 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg
     5821 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5881 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5941 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6001 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6061 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6121 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6181 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6241 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     6301 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     6361 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     6421 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     6481 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     6541 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     6601 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     6661 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6721 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     6781 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6841 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6901 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6961 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     7021 cgtc
//
