LOCUS       Exported                7147 bp DNA     circular SYN 02-SEP-2003
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 21, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        232..819
                     /label=CMV promoter
                     /label=CMV\promoter
                     /note="/invitrogen=2180000"
                     /note="/vntifkey=29"
     primer_bind     769..789
                     /label=CMV forward primer
     primer_bind     863..882
                     /label=T7 primer
     promoter        863..879
                     /label=T7 promoter
                     /label=T7\promoter
                     /note="/invitrogen=2370000"
                     /note="/vntifkey=30"
     CDS             896..2714
                     /codon_start=1
                     /label=Insert
                     /note="CI:V1=NheI,V2=XbaI,I1=NheI,I2=XbaI"
                     /translation="LAWATTTITTCPRARNCSPAWCPSWWNWMAT*TATSSACPARAKA
                     TPHMAS*P*SSSAPPASCPCLGLPS*PHCLGACSASPDTPTI*SSTTSSRAPCPRATCR
                     NGPSSLRTTATTRPGPK*SSRATPS*TGSS*RASTSKRTATSWATSWSTTPSTATCTSP
                     PTSRRTASRPTSA*TATSRTAPCSWPTTTSRTPPSEMAPCCCPTTTT*APRAS*ARTPT
                     RSGTTWCCSSS*QPPESPWAWTSCTRALAAAEEVADQAAVGDLALKPLPKKPPLKRQLQ
                     KRLQLKKPQQKKLLPRPEAADLGDQGEAEAQAAQGLKLLLKRPLPKKQLPKKLQQKRLP
                     QKRQPPRLDLAALGAVAAPAEAEDPACQRGKNCLQGWCLFWWNWTGM*MGISSPCLGRG
                     KGMLPTEN*H*SLSVQQGNCLCPGPHS*QPLATA*CVSPATLII*SSMIFSNPLCLRDM
                     CRKEQFSSRMMGITKHALK*NLRGIHS*IALN*RGLISKRTGIFWGTNWSITTTATMCI
                     SWLISRKMGSK*TSRSGTISRMEVCSWLIIISRILLSATDLCCCLIITICPTRAPCPRT
                     LMRKGIIWCCWNL*PLLASHWEWMNCISNL"
     primer_bind     complement(2741..2758)
                     /label=BGH reverse primer
     polyA_site      2747..2971
                     /label=BGH pA
                     /label=BGH\pA
                     /note="/invitrogen=1870000"
                     /note="/vntifkey=26"
     rep_origin      3017..3445
                     /label=f1 origin
                     /label=f1\origin
                     /note="/invitrogen=2500000"
                     /note="/vntifkey=33"
     promoter        3450..3819
                     /label=SV40 early promoter
                     /label=SV40\early\promoter
                     /note="/invitrogen=2290000"
                     /note="/vntifkey=29"
     CDS             3855..4649
                     /codon_start=1
                     /label=Neo(R)
                     /note="/invitrogen=1260000"
                     /note="/vntifkey=4"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4823..4953
                     /label=SV40 pA
                     /label=SV40\pA
                     /note="/invitrogen=1840000"
                     /note="/vntifkey=25"
     rep_origin      complement(5336..6006)
                     /direction=LEFT
                     /label=pUC origin
                     /label=pUC\origin
                     /note="/invitrogen=2540000"
                     /note="/vntifkey=33"
     CDS             complement(6151..7011)
                     /codon_start=1
                     /label=Amp(R)
                     /note="/invitrogen=1020000"
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7012..7110)
                     /label=bla promoter
                     /label=bla\promoter
                     /note="/invitrogen=2330000"
                     /note="/vntifkey=30"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 atgggccacc accaccatca ccacgtgtcc aagggcgagg aactgttcac cggcgtggtg
      961 cccatcctgg tggaactgga tggcgacgtg aacggccaca agttcagcgt gtccggcgag
     1021 ggcgaaggcg acgccacata tggcaagctg accctgaagt tcatctgcac caccggcaag
     1081 ctgcccgtgc cttggcctac cctcgtgacc acactgtctt ggggcgtgca gtgcttcgcc
     1141 agataccccg accatatgaa gcagcacgac ttcttcaaga gcgccatgcc cgagggctac
     1201 gtgcaggaac ggaccatctt ctttaaggac gacggcaact acaagaccag ggccgaagtg
     1261 aagttcgagg gcgacaccct cgtgaaccgg atcgagctga agggcatcga cttcaaagag
     1321 gacggcaaca tcctgggcca caagctggag tacaacgcca tccacggcaa cgtgtacatc
     1381 accgccgaca agcagaagaa cggcatcaag gccaacttcg gcctgaactg caacatcgag
     1441 gacggctccg tgcagctggc cgaccactac cagcagaaca cccccatcgg agatggcccc
     1501 gtgctgctgc ccgacaacca ctacctgagc acccagagca agctgagcaa ggaccccaac
     1561 gagaagcggg accacatggt gctgctcgag ttcgtgacag ccgccggaat caccctgggc
     1621 atggacgagc tgtacaaggg ctctggcggc agcggaggaa gtggcggatc aggcggcagt
     1681 gggggatctg gcgctgaagc cgctgccaaa gaagccgccg ctaaagaggc agctgcaaaa
     1741 gaggctgcag ctaaagaagc cgcagcaaaa gaagctgctg ccaaggccgg aagcggcgga
     1801 tctgggggat cagggggaag cggaggctca ggcggctcag gggctgaagc tgctgctaaa
     1861 gaggccgctg ccaaagaagc agctgccaaa gaagctgcag caaaagaggc tgccgcaaaa
     1921 gaggcagccg ccaaggctgg atctggcggc tctgggggca gtggcggctc cggcggaagc
     1981 ggaggatccg gcgtgtcaaa gggggaagaa ctgtttacag gggtggtgcc tattctggtg
     2041 gaactggacg gggatgtgaa tgggcataag ttctccgtgt ctggggaggg ggaaggggat
     2101 gctacctacg gaaaactgac actgaagttt atctgtacaa cagggaaact gcctgtgccc
     2161 tggcccacac tcgtgacaac ctttggctac ggcctgatgt gtttcgcccg ctaccctgat
     2221 catatgaagc agcatgattt tttcaaatcc gctatgcctg agggatatgt gcaggaaaga
     2281 acaattttct tcaaggatga tgggaattac aaaacacgcg ctgaagtgaa atttgagggg
     2341 gatacactcg tgaatcgcat tgaactgaag gggattgatt tcaaagagga cgggaatatt
     2401 ctggggcaca aactggagta taactacaac agccacaatg tgtatatcat ggctgataag
     2461 cagaaaaatg ggatcaaagt gaacttcaag atccggcaca atatcgagga tggaagtgtg
     2521 cagctggctg atcattatca gcagaatact cctatcggcg acggacctgt gctgctgcct
     2581 gataatcact atctgtccta ccagagcgcc ctgtccaagg accctaatga gaaaagggat
     2641 catatggtgc tgctggaatt tgtgaccgct gctggcatca cactgggaat ggatgaactg
     2701 tataagtaat ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     2761 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     2821 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     2881 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     2941 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     3001 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3061 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3121 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3181 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3241 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3301 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     3361 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3421 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     3481 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3541 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3601 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3661 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3721 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     3781 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     3841 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     3901 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     3961 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4021 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4081 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4141 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4201 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4261 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4321 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     4381 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     4441 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     4501 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     4561 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     4621 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     4681 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     4741 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     4801 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     4861 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4921 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     4981 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5041 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5101 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5161 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5221 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5281 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5341 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5401 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5461 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5521 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5581 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5641 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5701 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5761 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5821 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5881 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt
     5941 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6001 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6061 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6121 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6181 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6241 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6301 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6361 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6421 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6481 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6541 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6601 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6661 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6721 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6781 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6841 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6901 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6961 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7021 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7081 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7141 tgacgtc
//
