LOCUS       Exported               12571 bp DNA     circular SYN 06-APR-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12571)
  AUTHORS   Yanfei Zhang
  TITLE     Direct Submission
  JOURNAL   Exported Jun 22, 2021 from SnapGene 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12571
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    38..538
                     /label=ScHIS3 5' end
                     /note="ScHIS3 5' end"
     misc_feature    38..501
                     /label=ScHIS upstream flanking (-464/-1)
     promoter        314..501
                     /gene="S. cerevisiae HIS3"
                     /label=ScHIS3 promoter
     misc_feature    502..1915
                     /label=CG HIS3 w promoter and terminator
     misc_feature    502..615
                     /label=CgHIS3 promoter
     gene            complement(555..1184)
                     /label=CgHIS3
                     /note="CgHIS3"
     misc_feature    616..1248
                     /label=CgHIS3
                     /note="Candida glabrata imidazoleglycerol-phosphate 
                     dehydratase (HIS3) gene, complete cds"
     primer_bind     859..878
                     /label=Yfz_Seq23
                     /note="IntSeq_CgHIS3_middle_forward"
     misc_feature    1249..1915
                     /label=Cg HIS3 terminator
     primer_bind     1761..1780
                     /label=Yfz_Seq24
                     /note="IntSeq_CgHIS3  terminator_middle_forward"
     misc_feature    1921..1933
                     /label=SGI-S2
                     /note="SGI-S2"
     misc_feature    1934..1959
                     /label=SGI-S2
                     /note="SGI-S2"
     misc_feature    1964..2129
                     /label=adh1t
                     /note="adh1t"
     primer_bind     2116..2144
                     /label=Gal4_REV
     misc_feature    complement(2133..2138)
                     /label=STOP
                     /note="STOP"
     misc_feature    complement(2145..2858)
                     /label=GFP
                     /note="GFP"
     misc_feature    2145..2858
                     /label=MCS
                     /note="MCS"
     misc_feature    2859..3340
                     /label=MCS
                     /note="poly-linker region"
     primer_bind     2859..2909
                     /label=Gal1p_REV
     primer_bind     complement(2859..2880)
                     /label=Gal4_FOR
     misc_feature    complement(2862..2874)
                     /label=KOZAK
                     /note="KOZAK"
     misc_feature    complement(2883..3335)
                     /label=GAL1p
                     /note="ScGAL1 Promoter
                     http://rulai.cshl.edu/cgi-bin/SCPD/getgene2?GAL1"
     promoter        complement(2894..3335)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    3218..3335
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(3316..3340)
                     /label=Gal1p_FOR
     misc_feature    3336..3340
                     /label=SGI-S1
                     /note="SGI-S1"
     misc_feature    3341..3342
                     /label=SGI-S2
                     /note="SGI-S2"
     misc_feature    3347..3639
                     /label=ACT1 t
                     /note="ACT1 t"
     primer_bind     3640..3674
                     /label=Gal4_REV
     misc_feature    complement(3643..3648)
                     /label=STOP
                     /note="STOP"
     misc_feature    3648
                     /label=MCS
                     /note="MCS"
     misc_feature    3655..6297
                     /label=Gal4
     primer_bind     complement(6273..6319)
                     /label=Gal4_FOR
     misc_feature    6298..6545
                     /label=MCS
                     /note="poly-linker region"
     misc_feature    complement(6301..6313)
                     /label=KOZAK
                     /note="KOZAK"
     primer_bind     6314..6343
                     /label=C120_TATA_REV
     misc_feature    6322..6540
                     /label=MCS
                     /note="poly-linker region"
     promoter        complement(6354..6385)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    6398..6517
                     /label=C120 Repeats
     misc_feature    6541..6545
                     /label=SGI-S1
                     /note="SGI-S1"
     misc_feature    6546..6551
                     /label=SGI-S2
                     /note="SGI-S2"
     misc_feature    complement(6552..6803)
                     /label=CYC1 Terminator
                     /note="CYC1 Terminator"
     misc_feature    complement(6804..6809)
                     /label=STOP
                     /note="STOP"
     misc_feature    6809..7718
                     /label=MCS
                     /note="MCS"
     misc_feature    6816..6821
                     /label=pJLA Homology Arm
     misc_feature    6816..6821
                     /label=MCS
                     /note="poly-linker region"
     misc_feature    complement(6822..7451)
                     
     misc_feature    7458..7691
                     /label=VP16 Binding Domain
     CDS             complement(7692..7712)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7713..8465
                     /label=MCS
                     /note="poly-linker region"
     primer_bind     complement(7713..7734)
                     /label=Gal4_FOR
     misc_feature    complement(7716..7728)
                     /label=KOZAK
                     /note="KOZAK"
     misc_feature    complement(7739..8456)
                     /label=PGK1 Promoter
                     /note="PGK1 Promoter"
     misc_feature    8457..8465
                     /label=SGI-S1
                     /note="SGI-S1"
     primer_bind     complement(8535..8565)
                     /label=YfzOli29
                     /note="SacI_insert  Fragment_PstI-RCan be used for 
                     sequencing"
     misc_feature    8546..9081
                     /label=ScHIS3 downstream flanking (+1/+536)
     misc_feature    8763..9081
                     /label=ScHIS3 3' end
                     /note="ScHIS3 3' end"
     promoter        complement(9120..9138)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9145..9161)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      9302..9757
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     9932..9960
                     /label=Yfz_Oli27
                     /note="F-XhoI-Scar out the  SalI in plasmid P1"
     primer_bind     complement(10333..10357)
                     /label=Yfz_Oli26
                     /note="R-MluI-Scar out the AscI in plasmid P1"
     promoter        10394..10498
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    10499..11357
                     /label=AmpR
                     /note="AmpR"
     primer_bind     complement(10501..10520)
                     /label=Yfz_Seq25
                     /note="AmpR 5'  rev direction seq"
     misc_feature    12394..12515
                     /label=lac Promoter
                     /note="lac Promoter"
     protein_bind    12406..12427
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    12480..12496
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12504..12520
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        12541..12560
                     /label=T3_P
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgct gcacggtcct gttccctagc
       61 atgtacgtga gcgtatttcc ttttaaacca cgacgctttg tcttcattca acgtttccca
      121 ttgttttttt ctactattgc tttgctgtgg gaaaaactta tcgaaagatg acgacttttt
      181 cttaattctc gttttaagag cttggtgagc gctaggagtc actgccaggt atcgtttgaa
      241 cacggcatta gtcagggaag tcataacaca gtcctttccc gcaattttct ttttctatta
      301 ctcttggcct cctctagtac actctatatt tttttatgcc tcggtaatga ttttcatttt
      361 tttttttcca cctagcggat gactcttttt ttttcttagc gattggcatt atcacataat
      421 gaattataca ttatataaag taatgtgatt tcttcgaaga atatactaaa aaatgagcag
      481 gcaagataaa cgaaggcaaa gaggctctat atgtatataa agctgcggat gccttctgat
      541 ttaatctagt tctagtttac ttcaggttta cttaaagttg agataattga agcaaagaga
      601 agcatcgagc aaaggatggc gtttgttaag agggttacgc aggagacgaa tatacagctg
      661 gcgctggatc ttgacggtgg gtctgtttct gtacgggaga gcatactggg caaggaatat
      721 gctagtggtg atgggcagac catccatgtg cacactggag ttgggttttt ggaccacatg
      781 ttgactgcgc tggcgaagca tggcgggtgg tctctgatcc tggagtgtat aggggacttg
      841 cacatcgatg accaccacac tgttgaggac tgtgggattg cgctgggcca agcgttcaag
      901 gaggcgcttg gctccgtgcg tggtatcaag aggttcgggc atgggtttgc accactggac
      961 gaggcgctga gccgcgctgt ggttgacttc tccaataggc ctttcgccgt ggtggagctg
     1021 ggcctgaagc gagaacgcat aggccagcta tccacagaga tgatcccgca cttcttggag
     1081 agtttcgcca ctgaggcgcg tatcaccatg catgtggact gtctgcgggg caccaacgac
     1141 caccaccgct ccgaatcagc tttcaaggcg ctcgccatcg ccatcagaga ggcaagaaca
     1201 cctacgggtc gcgatgacgt tccatccact aagggtgtcc tagcatagaa cacagcccac
     1261 agctaccacc atcaacaata tttatataga taacgtacac atagaaatca cacaaacaga
     1321 gtatttattc ttaactacat gaactaccat cagaccgtct gggccactat ataatgtgct
     1381 attcataaac gtgatcactc tacgtagcag gcgaccccag gtgaaaattt ttcagcgagc
     1441 tgccagattg tcaggtgaaa aactgaaaaa aacttctggg cgatgagctt gtggcgggaa
     1501 attaagtata taaagcagtt agtttcttct gcttcttgtg gttctgggta ttcttgtaat
     1561 atttggaata atagaaaacc aagactactg cctttctttt catattatag aggaagaaat
     1621 acgcacgaac acgatataga ggtaaatttt ttgcaaaaag ataccaaact ataaaagaaa
     1681 tagaagacta aactacaaaa aataaagaaa gtaatacata tatccaatat gactgagtta
     1741 gcagagcaag tttccatgcc tgaagggcaa caaagacgtg gtggttacgt tccacctcac
     1801 atccgtaacg gtggtggcag aagaggcgat tctgctcctc gtggtggttt caacaacggt
     1861 ggcggccgtg gtggtttctt cagaaacaac ggtgaccgtg gtggattcgg ctttgggtac
     1921 ctccggatat gttggcgcgc cttaatttat tcgccggcga taagagcgac ctcatgctat
     1981 acctgagaaa gcaacctgac ctacaggaaa gagttactca agaataagaa ttttcgtttt
     2041 aaaacctaag agtcacttta aaatttgtat acacttattt tttttataac ttatttaata
     2101 ataaaaatca taaatcataa gaaattcgct gatcattact cgagtttgta tagttcatcc
     2161 atgccatgtg taatcccagc agctgttaca aactcaagaa ggaccatgtg gtctctcttt
     2221 tcgttgggat ctttcgaaag ggcagattgt gtggacaggt aatggttgtc tggtaaaagg
     2281 acagggccat cgccaattgg agtattttgt tgataatggt ctgctagttg aacgcttcca
     2341 tcttcaatgt tgtgtctaat tttgaagtta actttgattc cattcttttg tttgtctgcc
     2401 atgatgtata cattgtgtga gttatagttg tattccaatt tgtgtccaag aatgtttcca
     2461 tcttctttaa aatcaatacc ttttaactcg attctattaa caagggtatc accttcaaac
     2521 ttgacttcag cacgtgtctt gtagttcccg tcatctttga aaaatatagt tctttcctgt
     2581 acataacctt cgggcatggc actcttgaaa aagtcatgcc gtttcatatg atctgggtat
     2641 cttgaaaagc attgaacacc ataagtgaaa gtagtgacaa gtgttggcca tggaacaggt
     2701 agttttccag tagtgcaaat aaatttaagg gtaagttttc cgtatgttgc atcaccttca
     2761 ccctctccac tgacagaaaa tttgtgccca ttaacatcac catctaattc aacaagaatt
     2821 gggacaactc cagtgaaaag ttcttctcct ttgtcgacgc tagccatgat tttagcggcc
     2881 gctatagttt tttctccttg acgttaaagt atagaggtat attaacaatt ttttgttgat
     2941 acttttatga catttgaata agaagtaata caaactgaaa atgttgaaag tattagttaa
     3001 agtggttatg cagcttttcc atttatatat ctgttaatag atcaaaaatc atcgcttcgc
     3061 tgattaatta ccccagaaat aaggctaaaa aactaatcgc attatcatcc tatggttgtt
     3121 aatttgattc gttaatttga aggtttgtgg ggccaggtta ctgccaattt ttcctcttca
     3181 taaccataaa agctagtatt gtagaatctt tattgttcgg agcagtgcgg cgcgaggcac
     3241 atctgcgttt caggaacgcg accggtgaag acgaggacgc acggaggaga gtcttccgtc
     3301 ggagggctgt cgcccgctcg gcggcttcta atccgcccgg cgataaatat gatacacggt
     3361 ccaatggata aacatttttt atcaacacta tgatatataa atataatagt ttttcgtata
     3421 tatattcctt tttttggtca atttttgaaa ttttcgtaga aaagggagag acaaaacaca
     3481 ttatatcaat gaaaacgtac aaaaagtaga taaagtcagt gcttaaacac gtcttttcct
     3541 taaaaatact ttattatttt tattttattg agagggtggt ttaaaaatag aaatagagag
     3601 agaggtacat acataaacat acgcgcacaa aagcagagat gatcattact cgagctcttt
     3661 ttttgggttt ggtggggtat cttcatcatc gaatagatag ttatatacat catccattgt
     3721 agtggtatta aacatccctg tagtgattcc aaacgcgtta tacgcagttt ggtccgtcca
     3781 accaggtgac agtggttttg aattattacc atcatcaatt ttactagccg tgatttcatt
     3841 attcatgaag ttatcatgaa cgttagagga ggcaattggt tgtgaaagcg cttgagaatt
     3901 tgtttgagtt gttatgaggt tcggaccgtt gctactgtta gtgaaagtga aggacaatga
     3961 gctatcagca atattcccac tttgattaaa attggcgcca ccaaacaaag cagacggggt
     4021 cagtggcact aatgattgca gctgttgctg ttgccctaga aaaggcgtga ctgagcgatg
     4081 cgaaggtgtg cttcttggta ttgtcactgg agagttacga gagggtggac ggttagataa
     4141 cagcttgact agatcactga aacttgctcc tgatttcaat ggcacaggtg aaggccctac
     4201 tgagccagga gaaacatatt taacactgat attgttgaca ttttcctccg gaagagtagg
     4261 gtattgggcg atagttgcag aaccgacaat atttttaatg gcgctaccat tactattgtt
     4321 ataactgata tgcggtaatg ggattgcaca ctgtgataac agaaacggcg cacatacctc
     4381 ttccagtact tgaatgtatt tttcacaagt ctggatttta aaagtggcca gtttttttaa
     4441 tagcatcaga acagtgttaa tttgttgtaa taattgtgcg gtctcgttat tctcagcatt
     4501 cgattttgag tttgagagta gagtctttat gggtactagg actgcattga acaagtaata
     4561 agaacaattc caggcaaaat atggggtgac attatgattg tccatatagc tacttacaga
     4621 cataacagtt ctttgtgctg catcgcttaa catgatggag catcgtttaa cttcataact
     4681 ttgatgatca ttttgatcct gttctagttg tgactttttc tgggtaaaat tagtgaaaaa
     4741 atctcttaat acataaatga taagagacaa ctgtttccac ttcagttcga atcttgtaaa
     4801 ggatagccaa gggtgttcct tcaacaaatt ggttagagcg gtggtggaaa tatccatttg
     4861 taaaaacttt ggtgcctgtc tcgaaacctc ctcaatctca ttacaaatca tcaagcattt
     4921 ttttgcacat ataggacttt tttctgcagt tactgttttg tctagttcat agatttttgt
     4981 gaaaacttgt aagagccttg ctgtttcaat gatgccatga tatatggtgg gacctgttgt
     5041 ggtacgctgc acatcgtcga cagaagaagg gaaggagatt gtattctgag aaagctggat
     5101 ggatcgacca taaagcaggg acaattggat ctcccaagag tagacagacc accaaattcg
     5161 gcgtctttgt tccagaatgc tgctatcact gaaggacgag gggaggtccc tattcaagcc
     5221 caatgatatg gccattctta tggaaaagct gtgaaaatta tagctagtat ttgttttctg
     5281 cctccactgt gtatatcgcg acagaagatg tagggctgtc accaaaatta tggaacctga
     5341 ctcgaagacc ttgctcgtca aatgagattt agcattttga tagtaaaaaa catctatatc
     5401 agtagattcc ccctctatac accaggctcc aatggctaat atgcagttaa aaaggatttg
     5461 ccattgatcc ttcgacgcga tttcaatctg gttattatac aacatcatta gcgtcggtga
     5521 gtgcacgata gggcagtagg ggtgaaaatt attgagataa ctttgaagta aacgggatgt
     5581 tgtggatcta gaagccaacg tgtatctatc cgtaatcatg gtcgggagcc tgttaacgtt
     5641 agagttcgtg taattttccg gtttaaagcc aatagatcga agaatacata agagagaacc
     5701 gtcgccaaag aacccattat tgttggggtc cgttttcagg aagggcaagc catccgacat
     5761 gtcatcctct tcagaccaat caaatccatg aagagcatcc ctgggcataa aatccaacgg
     5821 aattgtggag ttatcatgat gagctgccga gtcaatcgat acagtcaact gtctttgacc
     5881 tttgttacta ctctcttccg atgatgatgt cgcacttatt ctatgctgtc tcaatgttag
     5941 aggcatatca gtctccactg aagccaatct atctgtgacg gcatctttat tcacattatc
     6001 ttgtacaaat aatcctgtta acaatgcttt tatatcctgt aaagaatcca ttttcaaaat
     6061 catgtcaagg tcttctcgag gaaaaatcag tagaaatagc tgttccagtc tttctagcct
     6121 tgattccact tctgtcagat gtgccctagt cagcggagac cttttggttt tgggagagta
     6181 gcgacactcc cagttgttct tcagacactt ggcgcacttc ggtttttctt tggagcactt
     6241 gagcttttta agtcggcaaa tatcgcatgc ttgttcgata gaagacagta gcttcatgct
     6301 agccatgatt ttagcggccg cggtggcttt accaacagta ccggattgcc aagctggaag
     6361 tcgagcttcc attatatacc ctctagtgtc tcttaagcat ggactaaagg ctacctataa
     6421 cgcatggact aaaggctacc tataacgcat ggactaaagg ctacctataa cgcatggact
     6481 aaaggctacc tataacgcat ggactaaagg ctacctactc gaagctggcg aactcacgta
     6541 cccggcgata aggccgcaaa ttaaagcctt cgagcgtccc aaaaccttct caagcaaggt
     6601 tttcagtata atgttacatg cgtacacgcg tctgtacaga aaaaaaagaa aaatttgaaa
     6661 tataaataac gttcttaata ctaacataac tataaaaaaa taaataggga cctagacttc
     6721 aggttgtcta actccttcct tttcggttag agcggatgtg gggggagggc gtgaatgtaa
     6781 gcgtgacata actaattaca tgatcattac tcgaggtcga cttaaatacc tgcttctacg
     6841 gcaattctaa ctaaatctgc actcgttttt aagtttaatt tttccattac caaacctctg
     6901 tgcattttga cagttttttc tgaaagaccc agtctggcag ccacttcttt atttcttagg
     6961 cctgatgcaa ccagagttgt gacttctaat tgacgtggtg aaagagtttt taacatctcc
     7021 gcagcacgtt ctctacgggc catgcccatg ttcggttgat cgtcatccac ttccacttgg
     7081 ctaccaagga agtatagtaa ttcatcatcg tcatcataga ttggtgccac caaaactgca
     7141 ttacgaaaag gagtaccatc ctttttgtaa ttcaaaatct caaccaacac gggtttatgc
     7201 tcacgaacac cttgacggat cttatctgtt agccagggtt cagtaccaga tcctgccaaa
     7261 aatctacaat tacgaccaac acattcttcc tcagaatacc cagttagatc ggtaaaggcc
     7321 tggttgatag caatcaaagg attatcagcc aatctgggat cactaacaac acttgctatg
     7381 ggtgaggcct cgatcaaatc caatacccat tgggctggag gttggacttc aactctagta
     7441 tcatctgctc cgaattcacc tccatattcg tcgataccaa gtgcatcagt aaacatctgt
     7501 tcaaattcga aatctgccat gtccaacgcg ccatacggag cagaatcatg tggtgtaaat
     7561 ccggggccgg gagaatcccc atcacctaac atgtccagat caaaatcatc aagagcatcg
     7621 gcatgtgcca tagctacatc ctcaccgtct aaatgtaatt cgtcaccaag tgaaacgtcc
     7681 gttggtgggg ctacttttct cttttttttc gggctagcca tgattttagc ggccgccacc
     7741 gcggtgtttt atatttgttg taaaaagtag ataattactt ccttgatgat ctgtaaaaaa
     7801 gagaaaaaga aagcatctaa gaacttgaaa aactacgaat tagaaaagac caaatatgta
     7861 tttcttgcat tgaccaattt atgcaagttt atatatatgt aaatgtaagt ttcacgaggt
     7921 tctactaaac taaaccaccc ccttggttag aagaaaagag tgtgtgagaa caggctgttg
     7981 ttgtcacacg attcggacaa ttctgtttga aagagagaga gtaacagtac gatcgaacga
     8041 actttgctct ggagatcaca gtgggcatca tagcatgtgg tactaaaccc tttcccgcca
     8101 ttccagaacc ttcgattgct tgttacaaaa cctgtgagcc gtcgctagga ccttgttgtg
     8161 tgacgaaatt ggaagctgca atcaatagga agacaggaag tcgagcgtgt ctgggttttt
     8221 tcagttttgt tctttttgca aacaaatcac gagcgacggt aatttctttc tcgataagag
     8281 gccacgtgct ttatgagggt aacatcaatt caagaaggag ggaaacactt cctttttctg
     8341 gccctgataa tagtatgagg gtgaagccaa aataaaggat tcgcgcccaa atcggcatct
     8401 ttaaatgcag gtatgcgata gttcctcact ctttccttac tcacgagtaa ttcttgcagc
     8461 tgtttcccgg gagctggaac tcccacctgc aacatgcggg atcctgagta aataagcggc
     8521 cgccaccgcg gtggagctca gatcttgaca ccgattattt aaagctgcag catacgatat
     8581 atatacatgt gtatatatgt atacctatga atgtcagtaa gtatgtatac gaacagtatg
     8641 atactgaaga tgacaaggta atgcatcatt ctatacgtgt cattctgaac gaggcgcgct
     8701 ttcctttttt ctttttgctt tttctttttt tttctcttga actccagaaa aaaaatataa
     8761 aagagatgga ggaacgggaa aaagttagtt gtggtgatag gtggcaagtg gtattccgta
     8821 agaacaacaa gaaaagcatt tcatattatg gctgaactga gcgaacaagt gcaaaattta
     8881 agcatcaacg acaacaacga gaatggttat gttcctcctc acttaagagg aaaaccaaga
     8941 agtgccagaa ataacagtag caactacaat aacaacaacg gcggctacaa cggtggccgt
     9001 ggcggtggca gcttctttag caacaaccgt cgtggtggtt acggcaacgg tggtttcttc
     9061 ggtggaaaca acggtggcag cggccggcca tttaaatgtt taaacggcca cgctggcccc
     9121 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     9181 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     9241 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     9301 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     9361 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     9421 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     9481 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     9541 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     9601 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     9661 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     9721 aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta
     9781 cgcatctgtg cggtatttca caccgcatag ggtaataact gatataatta aattgaagct
     9841 ctaatttgtg agtttagtat taggatctct acccttggcg aaaagtcctc tgccaacaat
     9901 gatgatatct gatccaccac ttacaacttc gtcgagggtt ctgtactgct gacccaatat
     9961 gcgtatatat accaatctaa gtctgtgctc cttccttcgt tcttccttct gttcggagat
    10021 taccgaatca aaaaaatttc aaggaaaccg aaatcaaaaa aaagaataaa aaaaaaatga
    10081 tgaattgaaa aggtggtatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
    10141 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
    10201 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
    10261 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
    10321 aatgtcatga taataatggt ttcttagacg tcacgcgccg aggacgtcag gtggcacttt
    10381 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
    10441 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    10501 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    10561 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
    10621 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
    10681 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    10741 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    10801 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    10861 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    10921 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    10981 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    11041 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    11101 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    11161 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    11221 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    11281 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    11341 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    11401 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    11461 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    11521 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    11581 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    11641 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    11701 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    11761 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    11821 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    11881 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    11941 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    12001 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    12061 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    12121 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    12181 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    12241 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    12301 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    12361 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    12421 cactcattag gcaccccagg ctttacactt tatgcttccg gctcctatgt tgtgtggaat
    12481 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcgg
    12541 aattaaccct cactaaaggg aacaaaagct g
//
