LOCUS       pGLOW39                10468 bp ds-DNA     circular     24-JUN-2021
DEFINITION  Cloning vector pUC19, complete sequence.
KEYWORDS    .
SOURCE      Cloning vector pUC19
  ORGANISM  Cloning vector pUC19 other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New England Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     To prepare vector for cloning: 
COMMENT     -Digest with ClaI and NgoMIV to fuse 5' homology arm directly to
            mScarlet, or 
COMMENT     -Digest with AvrII and NgoMIV to include a flexible linker (for
            C-terminal tags)
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    398..1010
                     /locus_tag="This sequence will be replaced by the homology
                     arm"
                     /label="This sequence will be replaced by the homology
                     arm"
                     /ApEinfo_label="This sequence will be replaced by the
                     homology arm"
                     /ApEinfo_fwdcolor="#cc9966"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(2750..2890,2938..3771)
                     /locus_tag="SQT-1"
                     /label="SQT-1"
                     /ApEinfo_label="SQT-1"
                     /ApEinfo_fwdcolor="#00cccc"
                     /ApEinfo_revcolor="#006666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             6080..7105
                     /note="hygromycinR"
                     /locus_tag="hygR"
                     /label="hygR"
                     /ApEinfo_label="hygR"
                     /ApEinfo_fwdcolor="#006666"
                     /ApEinfo_revcolor="#006666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1038..1886
                     /locus_tag="mScarlet-I-C1"
                     /label="mScarlet-I-C1"
                     /ApEinfo_label="mScarlet-I-C1"
                     /ApEinfo_fwdcolor="#ff6771"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(join(4144..4385,4437..4807,4859..5274))
                     /locus_tag="Cre"
                     /label="Cre"
                     /ApEinfo_label="Cre"
                     /ApEinfo_fwdcolor="#6699cc"
                     /ApEinfo_revcolor="#006666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_RNA        complement(8649..9201)
                     /note="RNAII transcript (complementary strand)"
                     /locus_tag="RNAII transcript (complementary strand)"
                     /label="RNAII transcript (complementary strand)"
                     /ApEinfo_label="RNAII transcript (complementary strand)"
                     /ApEinfo_fwdcolor="#cccc33"
                     /ApEinfo_revcolor="#999933"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(8649..9237)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
                     /locus_tag="pMB1 origin of replication (counter-clockwise)
                     (RN"
                     /label="pMB1 origin of replication (counter-clockwise)
                     (RN"
                     /ApEinfo_label="pMB1 origin of replication
                     (counter-clockwise) (RN"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_RNA        9091..9198
                     /note="RNAI transcript"
                     /locus_tag="RNAI transcript"
                     /label="RNAI transcript"
                     /ApEinfo_label="RNAI transcript"
                     /ApEinfo_fwdcolor="#cccc33"
                     /ApEinfo_revcolor="#999933"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7523..7534
                     /locus_tag="spacer"
                     /label="spacer"
                     /ApEinfo_label="spacer"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    7595..7624
                     /locus_tag="Myc Tag"
                     /label="Myc Tag"
                     /ApEinfo_label="Myc Tag"
                     /ApEinfo_fwdcolor="#cc33cc"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          1959..1964
                     /locus_tag="intron"
                     /label="intron"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(9411..10070)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(8631..9313)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          1914..1924
                     /locus_tag="intron(1)"
                     /label="intron(1)"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    7535..7564
                     /locus_tag="Myc Tag(1)"
                     /label="Myc Tag(1)"
                     /ApEinfo_label="Myc Tag"
                     /ApEinfo_fwdcolor="#ff99ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     -35_signal      9055..9060
                     /note="RNAI promoter (clockwise); TTGAAG"
                     /locus_tag="RNAI promoter (clockwise); TTGAAG"
                     /label="RNAI promoter (clockwise); TTGAAG"
                     /ApEinfo_label="RNAI promoter (clockwise); TTGAAG"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     -10_signal      9077..9082
                     /note="RNAI promoter (clockwise); GCTACA"
                     /locus_tag="RNAI promoter (clockwise); GCTACA"
                     /label="RNAI promoter (clockwise); GCTACA"
                     /ApEinfo_label="RNAI promoter (clockwise); GCTACA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     -35_signal      complement(9232..9237)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
                     /locus_tag="RNAII promoter (counter-clockwise); TTGAGA"
                     /label="RNAII promoter (counter-clockwise); TTGAGA"
                     /ApEinfo_label="RNAII promoter (counter-clockwise);
                     TTGAGA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     -10_signal      complement(9211..9216)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
                     /locus_tag="RNAII promoter (counter-clockwise); CGTAAT"
                     /label="RNAII promoter (counter-clockwise); CGTAAT"
                     /ApEinfo_label="RNAII promoter (counter-clockwise);
                     CGTAAT"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     -10_signal      complement(10312..10317)
                     /note="bla promoter (counter-clockwise); GAGACA"
                     /locus_tag="bla promoter (counter-clockwise); GAGACA"
                     /label="bla promoter (counter-clockwise); GAGACA"
                     /ApEinfo_label="bla promoter (counter-clockwise); GAGACA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_difference 3001..3001
                     /locus_tag="e1350"
                     /label="e1350"
                     /ApEinfo_label="e1350"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             661..966
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /locus_tag="ccdB"
                     /label="ccdB"
                     /ApEinfo_label="ccdB"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#006666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     5'UTR           join(2664..2691,2748..2749)
                     /locus_tag="sqt-1 5'UTR"
                     /label="sqt-1 5'UTR"
                     /ApEinfo_label="sqt-1 5'UTR"
                     /ApEinfo_fwdcolor="#ccffcc"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(9408..10268)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /locus_tag="beta-lactamase"
                     /label="beta-lactamase"
                     /ApEinfo_label="beta-lactamase"
                     /ApEinfo_fwdcolor="#00cccc"
                     /ApEinfo_revcolor="#006666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1965..1967
                     /locus_tag="spacer(1)"
                     /label="spacer(1)"
                     /ApEinfo_label="spacer"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    7565..7594
                     /locus_tag="Myc Tag(2)"
                     /label="Myc Tag(2)"
                     /ApEinfo_label="Myc Tag"
                     /ApEinfo_fwdcolor="#cc66cc"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           3772..3808
                     /locus_tag="sqt-1 3'UTR"
                     /label="sqt-1 3'UTR"
                     /ApEinfo_label="sqt-1 3'UTR"
                     /ApEinfo_fwdcolor="#cc3333"
                     /ApEinfo_revcolor="#cc3333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      1998..2379
                     /locus_tag="let-858 Terminator"
                     /label="let-858 Terminator"
                     /ApEinfo_label="let-858 Terminator"
                     /ApEinfo_fwdcolor="#cc0000"
                     /ApEinfo_revcolor="#cc0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(5275..5674)
                     /locus_tag="hsp16-41 promoter"
                     /label="hsp16-41 promoter"
                     /ApEinfo_label="hsp16-41 promoter"
                     /ApEinfo_fwdcolor="#0080c0"
                     /ApEinfo_revcolor="#0080c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     -35_signal      complement(10333..10338)
                     /note="bla promoter (counter-clockwise); TTCAAA"
                     /locus_tag="bla promoter (counter-clockwise); TTCAAA"
                     /label="bla promoter (counter-clockwise); TTCAAA"
                     /ApEinfo_label="bla promoter (counter-clockwise); TTCAAA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           7131..7482
                     /locus_tag="unc-54 3'UTR"
                     /label="unc-54 3'UTR"
                     /ApEinfo_label="unc-54 3'UTR"
                     /ApEinfo_fwdcolor="#990000"
                     /ApEinfo_revcolor="#cc0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           complement(3809..4140)
                     /locus_tag="tbb-2 3'UTR"
                     /label="tbb-2 3'UTR"
                     /ApEinfo_label="tbb-2 3'UTR"
                     /ApEinfo_fwdcolor="#cc3333"
                     /ApEinfo_revcolor="#993333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     1887..1907
                     /locus_tag="TEV site"
                     /label="TEV site"
                     /ApEinfo_label="TEV site"
                     /ApEinfo_fwdcolor="#ff6600"
                     /ApEinfo_revcolor="#ff6600"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2380..2749
                     /locus_tag="Psqt1"
                     /label="Psqt1"
                     /ApEinfo_label="Psqt1"
                     /ApEinfo_fwdcolor="#33cc00"
                     /ApEinfo_revcolor="#33cc00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          7517..7522
                     /locus_tag="intron(2)"
                     /label="intron(2)"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        5675..6037
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink;
                     /ApEinfo_revcolor=pink; /ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} width 5 offset 0"
                     /locus_tag="Prps-0"
                     /label="Prps-0"
                     /ApEinfo_label="Prps-0"
                     /ApEinfo_fwdcolor="#339933"
                     /ApEinfo_revcolor="#339933"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_structure  1011..1037
                     /locus_tag="Flexible Linker (will be deleted if ClaI is
                     used)"
                     /label="Flexible Linker (will be deleted if ClaI is used)"
                     /ApEinfo_label="Flexible Linker (will be deleted if ClaI
                     is used)"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8232..8238
                     /locus_tag="This sequence will be deleted if ClaI is used"
                     /label="This sequence will be deleted if ClaI is used"
                     /ApEinfo_label="This sequence will be deleted if ClaI is
                     used"
                     /ApEinfo_fwdcolor="#ffcc99"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7625..8231
                     /locus_tag="This sequence will be replaced by the homology
                     arm(1)"
                     /label="This sequence will be replaced by the homology
                     arm(1)"
                     /ApEinfo_label="This sequence will be replaced by the
                     homology arm"
                     /ApEinfo_fwdcolor="#cc9966"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(7669..7974)
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /locus_tag="ccdB(1)"
                     /label="ccdB(1)"
                     /ApEinfo_label="ccdB"
                     /ApEinfo_fwdcolor="#00cccc"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7483..7516
                     /locus_tag="lox2272"
                     /label="lox2272"
                     /ApEinfo_label="lox2272"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1925..1958
                     /locus_tag="lox2272(1)"
                     /label="lox2272(1)"
                     /ApEinfo_label="lox2272"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    1968..1994
                     /locus_tag="HA"
                     /label="HA"
                     /ApEinfo_label="HA"
                     /ApEinfo_fwdcolor="#6600ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(8243..8263)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     378..395
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          1191..1241
                     /locus_tag="intron(3)"
                     /label="intron(3)"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#eb606c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          1455..1505
                     /locus_tag="intron(4)"
                     /label="intron(4)"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#eb606c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          1677..1727
                     /locus_tag="intron(5)"
                     /label="intron(5)"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#eb606c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtTA CCCGAAGTAT
      421 GTCAAAAAGA GGTATGCTAT GAAGCAGCGT ATTACAGTGA CAGTTGACAG CGACAGCTAT
      481 CAGTTGCTCA AGGCATATAT GATGTCAATA TCTCCGGTTC GGTAAGCACA ACCATGCAGA
      541 ATGAAGCCCG TCGTCTGCGT GCCGAACGCT GGAAAGCGGA AAATCAGGAA GGGATGGCTG
      601 AGGTCGCCCG GTTTATTGAA ATGAACGGCT CTTTTGCCGA CGAGAACAGG GGCTGGTGAA
      661 ATGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA
      721 CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT
      781 CTGCTGTCAG ATAAAGTCCC CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC
      841 TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT CCGTTATCGG GGAAGAAGTG
      901 GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA
      961 ATATAAATGT CAGGCTCCCT TATACACAGC CAGTCTGCAG GTCGAcctag GGAGCATCGG
     1021 GAGCCTCAGG AGCATCGATG GTCTCCAAGG GAGAGGCCGT CATCAAGGAG TTCATGCGTT
     1081 TCAAGGTCCA CATGGAGGGA TCCATGAACG GACACGAGTT CGAGATCGAG GGAGAGGGAG
     1141 AGGGACGTCC ATACGAGGGA ACCCAAACCG CCAAGCTCAA GGTCACCAAG gtaagtttaa
     1201 acatatatat actaactaac cctgattatt taaattttca gGGAGGACCA CTCCCATTCT
     1261 CCTGGGACAT CCTCTCCCCA CAATTCATGT ACGGATCCCG TGCCTTCATC AAGCACCCAG
     1321 CCGACATCCC AGACTACTAC AAGCAATCCT TCCCAGAGGG ATTCAAGTGG GAGCGTGTCA
     1381 TGAACTTCGA GGACGGAGGA GCCGTCACCG TCACCCAAGA CACCTCCCTC GAGGACGGAA
     1441 CCCTCATCTA CAAGgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt
     1501 ttcagGTCAA GCTCCGTGGA ACCAACTTCC CACCAGACGG ACCAGTCATG CAAAAGAAGA
     1561 CCATGGGATG GGAGGCCTCC ACCGAGCGTC TCTACCCAGA GGACGGAGTC CTCAAGGGAG
     1621 ACATCAAGAT GGCCCTCCGT CTCAAGGACG GAGGACGTTA CCTCGCCGAC TTCAAGgtaa
     1681 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagACC ACCTACAAGG
     1741 CCAAGAAGCC AGTCCAAATG CCAGGAGCCT ACAACGTCGA CCGTAAGCTC GACATCACCT
     1801 CCCACAACGA GGACTACACC GTCGTCGAGC AATACGAGCG TTCCGAGGGA CGTCACTCCA
     1861 CCGGAGGAAT GGACGAGCTC TACAAGGAGA ATCTGTACTT TCAATCCGGA AAGgtaagtt
     1921 taaaATAACT TCGTATAGGA TACTTTATAC GAAGTTATtt tcagGGATAC CCATACGACG
     1981 TCCCAGACTA CGCCTAAGGA TGATCGACGC CaACGTCGTT GAATTTTCAA ATTTTAAATA
     2041 CTGAATATTT GTTTTTTTTC CTATTATTTA TTTATTCTCT TTGTGTTTTT TTTCTTGCTT
     2101 TCTAAAAAAT TAATTCAATC CAAATCTAAa catttttttt tctctttccg tctcccaatt
     2161 cgtattccgc tcctctcatc tgaacacaat gtgcaagttt atttatcttc tcgctttcat
     2221 ttcattagga cgtgggggga attggtggaa gggggaaaca cacaaaagga tgatggaaat
     2281 gaaataagga cacacaatat gcaacaacat tcaattcaga aatatggagg aaggtttaaa
     2341 agaaaacata aaaatatata gaggaggaag gaaaactagc tgccctcgat tgtctatctt
     2401 ccattctcac caagtttcaa caactttatt cagtcattct tctctggttc atttcccaac
     2461 caatcttccc gaataaagcc catctgtcct ttcggccaca ttcctcacct acatccgtac
     2521 tccttatctc ccgttcttat ccagtctttt atcgtctctg cgtctcgcac actgtctcgt
     2581 cggtaatgta aaaaaaaaac aaaaaaaact tgaaaacttg cggaaagatt tcctaataaa
     2641 aggagacgaa tccggtagga aatcgacaag tctctgcttc tcgaagacta ggtgagttac
     2701 atttctttgg ccgaattatt ttgaaatcct aaatttgata atttcagagA TGTCTGTAAA
     2761 ACTTGCGTGT TATGTGACGG CTTCGGTCAC TGTCGCCACT CTTATGGTAT GCTTCATGAC
     2821 CATGTCAACC ATCTACTCGG AGGTTGATGG ATTCAGAGAG AAGCTTGATA CCGAGATGAA
     2881 TGTGTTCAGA gtgagttaaa ttatattttg aattttaaat aatttttaat tttctagCAA
     2941 TCTACCAATG GATTGTGGAA GGACATAGTT GTCATCGGAA GATCTAGCAA GCGTGTCCGT
     3001 TGTCAATATG AAGAGACCAA CGCTACCCCA ACTCCACATG CTGATGGATC CCCATCTGCT
     3061 CCACCAGGTC AACCACCAGC AGTTCCACCA GTCTTCAACC AGCCAAAGAC TCCAAATGGA
     3121 GCCAATGGAA ATGGACCAAC CTGCAACTGC AATGCTGATA ACAAGTGCCC AGCTGGACCA
     3181 TCCGGACCAA AGGGAGTTCC AGGAGTTCCA GGACTCGACG GAGTTCCAGG ACTTGACGGT
     3241 GTTCCAGGAG TTGGAGCTGA TGATATCGCT CCACAACGCG AGTCTGTCGG ATGCTTCACT
     3301 TGCCCACAAG GACCAGTTGG ACCACCAGGA GCTCTTGGAA GACCAGGACC ACGTGGACTT
     3361 CCAGGACCAA GAGGACAAAA TGGAAACCCA GGAAGAGATG GACAACCAGG ACATCCAGGA
     3421 GAGCAAGGAT CATCCGGTCA AATCGGAAAG ATCGGAGAGC CAGGACCACC AGGAGAGAAG
     3481 GGACGCGACG CCGAGCATCC AATCGGAAGA CCAGGACCAA AGGGACCAAG AGGAGATCAA
     3541 GGACCAACAG GACCAGCTGG ACAGAACGGT CTTCACGGAC CACCAGGAGA GCCAGGAACC
     3601 GTTGGACCAG AAGGACCATC TGGAAAGCAA GGACGTCAAG GACCAGACGG AACCCAGGGA
     3661 GAGACTGGAC CAGACGGAAG ACCAGGAAAG GATGCCGAGT ACTGCCAGTG CCCAGACAAG
     3721 TCTCCACCAT CAGAGGCTGT CAACGCCAAC CGTGGATACA GAAATATCTA Aattgttggt
     3781 gttttctaat aaaatatttg agattcagtg agactttttt cttggcggca caataaagtt
     3841 tcctcttttt gttgggtcac tcaacgaaca caatacaaat atttgagcgc tcgcattttt
     3901 ctcaaccttt cgggtttaaa gcacttcaaa atggaattga agagagattg tgaaagtaca
     3961 tcagacagaa tcacattttg atagcattca cttcactcag atgcaaactt ttcagtgact
     4021 ataaaaagtc ataaaataat aacattaaaa aagcaaataa attagcgaga gaattttttg
     4081 acaaaaagaa agaagagtga tagagaagaa gggaatgctt gaaaggattt tgcatttatc
     4141 ttaATCACCG TCTTCGAGAA GACGAACCAT GGCGCCAGTT TCAGAATCCA AGTTTCTAAT
     4201 ATAGTTCATC ACAATGTTGA CGTTGGTCCA ACCTCCGGCT TGCATAATCT CTGGGATGGA
     4261 AACTCCAGCG CGAGCCATGT CACGGGCAGC TCCGACACGG GCGGAATGTC CAGACCAAGC
     4321 CAAGTAACGT TGTCCGGAAT CATCCTTGGC TCCGTAGATG AGACGGTGGG TAGCTTCGAA
     4381 GATTCctgaa aatttatttt cagcttattc ttagctaata tgttgtttaa acttacCTTC
     4441 AAGAGCTCGA GTCGAGAGTT GTGATGTTGC ACTCGGGGCA GCAACTCCGT TCTTGCGAAC
     4501 ACGACAGAAC AGGTAGTTGT TTGGATCATC GGCGACTCCG GAAACCGAAA TCCAGCGCTC
     4561 AACGAGCTTG GTAACTCCAA GGGAGAGCGC TTTTTCGACT CCGGCAGTCG AGACCAGCGT
     4621 TTTTGTACGT CCGATGTGGA TGAGCATTCT TCCTCCGTCT GTGCGACTAA TATCCTTGAC
     4681 GCGAATGCGG GCAATTTCGG CGATACGGAG AAGTGTGTTG TAAGCAATTC CGAGGAAGGC
     4741 GAGGTTTCGG ATGTCCTGGC AACGATCGGA ATTTTCCATA AGAGAACGGA CCTGATCGAA
     4801 GTCGGTTctg aaaatttcgt caagtcttag gattgaatac attaatattt aaacttacCT
     4861 TTCGAAGGCG AGGGCTTGCT TGGCTCTCTC TCCAGCATCA ACATTTTCCT TGCGGATACG
     4921 TCTCATTACG AGAGATACGG CATTAGAATC TGATGGGCGT GGAAGTCCAG ATCGGCGATG
     4981 GAGCATATTG AGCTGTCCGA GATGTTGTTG GATCGTCTTC ACCGCCAATC CACGAGCTTG
     5041 GAGGTAGAGC AAGTAGTCAC GAACGTCTTC TGGCTCGGCT GGGAACCACT TGCGGTTGTT
     5101 AAGCTTGCAC CAGGCAGCCC AAGAACGGCA CACTGAGAGA AGCATCTTCC AGGTGTGCTC
     5161 GGAGAAGGCT TGTCTGTCAC GGAACATATC CATGAGGTTC TTGCGAACTT CATCACTTGT
     5221 CGCGTCAACT GGAAGTGCTG GAAGATTTTG ATGAACAGTG AGAAGATTTG ACATattttc
     5281 gaagtttttt agatgcacta gaacaaagcg tgttggcttc ctctgagccc gctttcctta
     5341 tatacccgca ttctgcagcc ttacagaatg ttctagaagg tcctagatgc attcgtttga
     5401 aaatactccc ggtgggtgca aagagacgca gacggaaaat gtatctgggt ctctttattg
     5461 tgtacactac ttttccatgt accgaatgtg agtcgccctc cttttgcaac aagcagctcg
     5521 aatgttctag aaaaaggtgg aaaatagtat aaataccgtt gaaaataaat accgaacaac
     5581 atttgctcta attgtgaaat tagaaatctt caaactataa tcatgtcact ttaccactat
     5641 ttccgtccag ctcaacgttc cgtttttggt gatcATTTTT GCTTTCGTCG TAAATCTACA
     5701 CACGCGTCTC TTCCGTGCGA GAGTCCAAGC CAGCAGCCAA ATTCGTTGAC TGAGTATTCA
     5761 ACGTTTATAC GTTGTCGGCA ACGAGAAATA GGAAAATGCA TCGGGAAATG TTCTTTTTTC
     5821 GATTTTTTCC AAGGTTTTGA CAAATTTTAC CACGAATTTT GCTATGTTTT CAATTAAAAA
     5881 ATATGTTATT CAACTGTTTC TATGAGGAAA ATAAGGCTTT GCATGTAATT TTCTTATTCA
     5941 GCATAATTTT TAATTAATTT GAATTTTCTG TCCTAACGTT TATTTTGTTT TCTTGGTTAT
     6001 GACTGATCTG AAATTAATTT TTGAATTTTA AGGTAATATG TCAGGCGGTG CCGCAAGTTT
     6061 GTACAAAAAA GCAGGCTCCA TGAAAAAGCC TGAACTCACC GCGACGTCTG TCGAGAAGTT
     6121 TCTGATCGAA AAGTTCGACA GCGTCTCCGA CCTGATGCAG CTCTCGGAGG GCGAAGAATC
     6181 TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC CTGCGGGTAA ATAGCTGCGC
     6241 CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT GCATCGGCCG CGCTCCCGAT
     6301 TCCGGAAGTG CTTGACATTG GGGAATTCAG CGAGAGCCTG ACCTATTGCA TCTCCCGCCG
     6361 TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA CTGCCCGCTG TTCTGCAGCC
     6421 GGTCGCGGAG GCCATGGATG CGATCGCTGC GGCCGATCTT AGCCAGACGA GCGGGTTCGG
     6481 CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG CGTGATTTCA TATGCGCGAT
     6541 TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC GACACCGTCA GTGCGTCCGT
     6601 CGCGCAGGCT CTCGATGAGC TGATGCTTTG GGCCGAGGAC TGCCCCGAAG TCCGGCACCT
     6661 CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC AATGGCCGCA TAACAGCGGT
     6721 CATTGACTGG AGCGAGGCGA TGTTCGGGGA TTCCCAATAC GAGGTCGCCA ACATCTTCTT
     6781 CTGGAGGCCG TGGTTGGCTT GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC
     6841 GGAGCTTGCA GGATCGCCGC GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT
     6901 CTATCAGAGC TTGGTTGACG GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA
     6961 CGCAATCGTC CGATCCGGAG CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC
     7021 GGCCGTCTGG ACCGATGGCT GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG
     7081 CACTCGTCCG AGGGCAAAGG AATAGACCCA GCTTTCTTGT ACAAAGTGGG TCCAATTACT
     7141 CTTCAACATC CCTACATGCT CTTTCTCCCT GTGCTCCCAC CCCCTATTTT TGTTATTATC
     7201 AAAAAACTTC TCTTAATTTC TTTGTTTTTT AGCTTCTTTT AAGTCACCTC TAACAATGAA
     7261 ATTGTGTAGA TTCAAAAATA GAATTAATTC GTAATAAAAA GTCGAAAAAA ATTGTGCTCC
     7321 CTCCCCCCAT TAATAATAAT TCTATCCCAA AATCTACACA ATGTTCTGTG TACACTTCTT
     7381 ATGTTTTTTA CTTCTGATAA ATTTTTTTGA AACATCATAG AAAAAACCGC ACACAAAATA
     7441 CCTTATCATA TGTTACGTTT CAGTTTATGA CCGCAATTTT TAATAACTTC GTATAGGATA
     7501 CTTTATACGA AGTTATtttc agGGAGCCGG ATCTGAACAA AAACTTATTT CTGAAGAGGA
     7561 TCTTGAGCAA AAGCTCATCT CCGAGGAGGA CCTCGAGCAG AAGTTGATCA GCGAGGAAGA
     7621 CTTGccggcT CGACCTGCAG ACTGGCTGTG TATAAGGGAG CCTGACATTT ATATTCCCCA
     7681 GAACATCAGG TTAATGGCGT TTTTGATGTC ATTTTCGCGG TGGCTGAGAT CAGCCACTTC
     7741 TTCCCCGATA ACGGAGACCG GCACACTGGC CATATCGGTG GTCATCATGC GCCAGCTTTC
     7801 ATCCCCGATA TGCACCACCG GGTAAAGTTC ACGGGGGACT TTATCTGACA GCAGACGTGC
     7861 ACTGGCCAGG GGGATCACCA TCCGTCGCCC GGGCGTGTCA ATAATATCAC TCTGTACATC
     7921 CACAAACAGA CGATAACGGC TCTCTCTTTT ATAGGTGTAA ACCTTAAACT GCATTTCACC
     7981 AGCCCCTGTT CTCGTCGGCA AAAGAGCCGT TCATTTCAAT AAACCGGGCG ACCTCAGCCA
     8041 TCCCTTCCTG ATTTTCCGCT TTCCAGCGTT CGGCACGCAG ACGACGGGCT TCATTCTGCA
     8101 TGGTTGTGCT TACCGAACCG GAGATATTGA CATCATATAT GCCTTGAGCA ACTGATAGCT
     8161 GTCGCTGTCA ACTGTCACTG TAATACGCTG CTTCATAGCA TACCTCTTTT TGACATACTT
     8221 CGGGTtccta ggaaatcgat aacatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8281 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     8341 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     8401 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8461 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8521 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8581 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8641 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8701 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8761 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8821 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8881 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8941 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9001 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9061 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9121 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9181 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9241 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9301 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9361 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9421 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     9481 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9541 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9601 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9661 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9721 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9781 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9841 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9901 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9961 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10021 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10081 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10141 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10201 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10261 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10321 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10381 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
    10441 aataggcgta tcacgaggcc ctttcgtc   
//
