LOCUS       Exported                7141 bp DNA     circular SYN 22-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 25, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..1622
                     /label=CAGGS promoter
     misc_feature    1..360
                     /label=Cmv enhancer
     misc_feature    1725..1730
                     /label=KpnI
     CDS             1731..3050
                     /label=Translation 1731-3050
     misc_feature    1731..2018
                     /label=Ccl20
     misc_feature    2025..2078
                     /label=flexible linker
     misc_feature    2085..2090
                     /label=HpaI
     misc_feature    2091..2096
                     /label=AgeI
     misc_feature    2097..2204
                     /label=3xHA
     misc_feature    2205..2210
                     /label=AgeI
     misc_feature    2211..2216
                     /label=AflII
     misc_feature    2217..2218
                     /label=pre mgrasp
     misc_feature    2217..2218
                     /label=CD4
     misc_feature    2219..3041
                     /label=pre mgrasp
     misc_feature    2219..2876
                     /label=CD4
     misc_feature    2877..3041
                     /label=NXN cytoplasmic region
     misc_feature    3042..3047
                     /label=AflII
     misc_feature    3048..3050
                     /label=STOP CODON
     CDS             3051..3056
                     /label=Translation 3051-3056
     misc_feature    3828..4019
                     /label=BGH polyA
     primer_bind     complement(4640..4660)
                     /label=M13-rev
     misc_binding    complement(4666..4688)
                     /label=LacO
     misc_feature    5019..5147
                     /label=SV40 early polyA
     rep_origin      complement(5373..6055)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6153..6812)
                     /label=AmpR
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg ggcgagggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attcggtacc atgtgctgta
     1741 ccaagagttt gctcctggct gctttgatgt cagtgctgct actccacctc tgcggcgaat
     1801 cagaagcagc aagcaacttt gactgctgtc ttggatacac agaccgtatt cttcatccta
     1861 aatttattgt gggcttcaca cggcagctgg ccaatgaagg ctgtgacatc aatgctatca
     1921 tctttcacac aaagaaaaag ttgtctgtgt gcgcaaatcc aaaacagact tgggtgaaat
     1981 atattgtgcg tctcctcagt aaaaaagtca agaacatggg taccgggaac ggaaatggca
     2041 atggtaacgg gaatggaaac ggcaatggta acgggaatac tagtgttaac accggtgcgg
     2101 ccgtttaccc atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg
     2161 caggatccta tccatatgac gttccagatt acgctccggc cgccaccggt cttaaggtcg
     2221 actttcagaa agctagctct attgtttata agaaggaggg ggaacaggtg gagttcagtt
     2281 tcccattggc cttcacagtc gagaagctca caggatcagg agagttgtgg tggcaggccg
     2341 aaagagcctc ctccagcaag agttggatca cttttgatct gaaaaacaaa gaagtgagcg
     2401 tgaagagagt cacacaggac cctaagctgc agatgggcaa gaagttgcct ttgcacttga
     2461 cactccccca agccctgccc cagtatgctg gctccgggaa cctcaccctc gccttggagg
     2521 ctaagaccgg aaagttgcat caggaggtca acctcgtggt gatgagggca acacagctgc
     2581 agaagaacct gacctgcgaa gtgtggggtc ccacctcacc taaactgatg ctgtctctga
     2641 agctggagaa taaggaggct aaagtcagta aaagagagaa ggctgtgtgg gtccttaacc
     2701 cggaggcagg gatgtggcag tgtcttctta gcgactctgg acaggtgctg ctggagagca
     2761 acatcaaggt gcttcctact tggtcaacac cagtacagcc gatggccctg atcgtactcg
     2821 ggggagtcgc cggcctgctt ctgtttatcg gcctgggcat cttcttctgc gtacgctaca
     2881 agtatcgcaa cagggatgag ggatcttacc acgttgacga atctaggaac tatatcagta
     2941 acagcgctca gagtaatggc gccgtggtta aagagaaaca gccctccagc gccaaatcag
     3001 caaacaaaaa caagaaaaac aaggataaag agtactacgt acttaagtaa ctcgaggccg
     3061 caggtaagta tcaaggttac aagacaggtt taaggagacc acatagaaac tgggcttgtc
     3121 gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt
     3181 tgcctttctc tccacaggtg tcgacaatca acctctggat tacaaaattt gtgaaagatt
     3241 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3301 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3361 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     3421 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3481 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     3541 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     3601 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     3661 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     3721 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3781 ggccgcctcc ccgcctggaa ttcgagctcg gtacgatcag cctcgactgt gccttctagt
     3841 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     3901 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     3961 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4021 aggccagctt ttgttccctt tagggttaat tgcgcgcttg gcgctaatca tggtcatagc
     4081 tgtttccact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     4141 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     4201 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     4261 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     4321 acatcagaat gagtatttgg tttagagttt ggcaacatat gccatatgct ggctgccatg
     4381 aacaaaggtg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     4441 tattccatag aaaagccttg acttgaggtt agattttttt ttatattttg tttgtgttat
     4501 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     4561 tctcctgact actcccagtc atagctgtcc ctcttctctt atgaagatcc ctcgacctgc
     4621 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     4681 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     4741 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     4801 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4861 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4921 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     4981 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
     5041 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5101 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
     5161 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5221 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5281 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5341 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5401 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5461 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5521 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5581 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5641 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5701 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5761 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5821 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5881 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5941 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6001 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6061 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6121 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6181 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6241 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6301 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6361 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6421 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6481 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6541 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6601 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6661 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6721 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6781 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6841 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6901 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6961 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7021 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7081 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7141 g
//
