LOCUS       38_AAV_Syn_CCR6-eLOV-TE 6329 bp ds-DNA     circular     22-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /label="ITR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    151..628
                     /label="synapsin promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    636..641
                     /label="BaMHI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    642..647
                     /label="Kozak"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    648..1769
                     /label="CCR6"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1770..1775
                     /label="SpeI"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1776..1811
                     /label="NES"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1818..1823
                     /label="NheI"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1818..1823
                     /label="nhe1"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    1824..1829
                     /label="asLOV2"
                     /ApEinfo_revcolor=#f746ff
                     /ApEinfo_fwdcolor=#f746ff
     misc_feature    1830..1832
                     /label="L2R"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1833..1859
                     /label="asLOV2"
                     /ApEinfo_revcolor=#f746ff
                     /ApEinfo_fwdcolor=#f746ff
     misc_feature    1860..1862
                     /label="N12S"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1863..1907
                     /label="asLOV2"
                     /ApEinfo_revcolor=#f746ff
                     /ApEinfo_fwdcolor=#f746ff
     misc_feature    1908..1910
                     /label="A28V"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1911..2174
                     /label="asLOV2"
                     /ApEinfo_revcolor=#f746ff
                     /ApEinfo_fwdcolor=#f746ff
     misc_feature    2175..2177
                     /label="H117R"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2178..2183
                     /label="asLOV2"
                     /ApEinfo_revcolor=#f746ff
                     /ApEinfo_fwdcolor=#f746ff
     misc_feature    2184..2201
                     /label="asLOV2 Ja helix"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2184..2201
                     /label="asLOV2"
                     /ApEinfo_revcolor=#f746ff
                     /ApEinfo_fwdcolor=#f746ff
     misc_feature    2202..2204
                     /label="G528A"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2205..2213
                     /label="asLOV2"
                     /ApEinfo_revcolor=#f746ff
                     /ApEinfo_fwdcolor=#f746ff
     misc_feature    2205..2213
                     /label="asLOV2 Ja helix"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2214..2216
                     /label="I130V"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2217..2231
                     /label="asLOV2 Ja helix"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2217..2231
                     /label="asLOV2"
                     /ApEinfo_revcolor=#f746ff
                     /ApEinfo_fwdcolor=#f746ff
     misc_feature    2232..2234
                     /label="N538E"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2235..2252
                     /label="asLOV2 Ja helix"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2235..2252
                     /label="asLOV2"
                     /ApEinfo_revcolor=#f746ff
                     /ApEinfo_fwdcolor=#f746ff
     misc_feature    2253..2273
                     /label="TEVcs ENLYFQL"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2253..2273
                     /label="Translation 2253-2273"
     misc_feature    2280..2285
                     /label="BamHI"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    2286..2309
                     /label="Flag"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             2286..3320
                     /label="Translation 2286-3320"
     CDS             2310..3314
                     /label="tTA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2943..3314
                     /label="VP16"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(3315..3317)
                     /label="double stop"
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_fwdcolor=#800080
     CDS             3318..3320
                     /label="Translation 3318-3320"
     misc_feature    3321..3326
                     /label="EcoRI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3333..3564
                     /label="PolyA"
                     /ApEinfo_revcolor=#ff2549
                     /ApEinfo_fwdcolor=#ff2549
     misc_feature    3593..3733
                     /label="ITR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtTGTACA gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaaggatc cGCCACCATG AGCGGAGAGA
      661 GCATGAACTT CAGTGATGTG TTCGATTCCA GTGAGGACTA CTTCGTGTCC GTCAATACAT
      721 CCTACTACAG CGTAGATTCT GAGATGCTGC TTTGCTCCCT GCAGGAGGTG CGCCAGTTCT
      781 CCCGACTCTT CGTTCCCATC GCGTATTCAC TCATCTGCGT CTTTGGCCTC CTGGGGAATA
      841 TCTTGGTAGT CATCACATTT GCTTTTTATA AAAAGGCCAG ATCCATGACA GACGTCTACC
      901 TGCTTAATAT GGCCATTGCA GACATCCTGT TCGTCCTGAC GCTCCCATTC TGGGCTGTGA
      961 GTCACGCTAC CGGGGCCTGG GTGTTCAGTA ATGCAACCTG CAAGCTGTTG AAGGGAATTT
     1021 ATGCCATCAA CTTTAATTGC GGTATGCTGC TGCTGACTTG CATAAGTATG GACAGATACA
     1081 TCGCCATAGT ACAGGCAACA AAATCCTTCA GACTGAGGTC TCGAACGCTC CCTAGGTCTA
     1141 AAATCATTTG TCTTGTTGTA TGGGGCTTGA GCGTGATTAT TTCCAGCAGT ACTTTCGTGT
     1201 TTAACCAGAA GTACAACACG CAGGGCAGCG ATGTGTGTGA GCCCAAGTAC CAGACGGTAT
     1261 CTGAGCCTAT CAGGTGGAAG CTGTTGATGC TGGGTTTGGA ATTGCTGTTC GGCTTTTTTA
     1321 TCCCACTCAT GTTTATGATT TTTTGTTATA CCTTCATCGT GAAGACCCTG GTCCAGGCAC
     1381 AAAACTCTAA GCGACACAAG GCCATCCGGG TAATCATCGC TGTTGTGCTG GTATTTCTGG
     1441 CTTGCCAGAT CCCTCATAAT ATGGTCCTTC TGGTAACGGC AGCCAACCTG GGCAAAATGA
     1501 ATCGCTCATG TCAGTCAGAA AAGCTTATCG GGTACACCAA AACGGTTACG GAGGTTCTGG
     1561 CGTTCCTGCA TTGTTGCCTT AACCCTGTGC TGTACGCATT TATAGGTCAG AAGTTTCGAA
     1621 ACTACTTCCT GAAGATTCTC AAAGACCTCT GGTGTGTAAG AAGAAAGTAT AAAAGTAGTG
     1681 GATTTTCATG CGCAGGACGC TACTCCGAAA ACATTTCCAG ACAGACCAGT GAGACGGCCG
     1741 ATAACGATAA CGCCTCCTCC TTTACCATGA CTAGTGAGCT GGCCGAGAAG CTGGCCGGCC
     1801 TGGACATCAA CGGTGGAgct agcGGCAGCc ggGCGACCAC CCTGGAACGT ATTGAAAAGa
     1861 gcTTTGTGAT TACCGATCCG CGTCTGCCGG ATAACCCGAT TATTTTTgtg AGCGATAGCT
     1921 TTCTGCAGCT GACCGAATAT AGCCGTGAAG AAATTCTGGG CCGTAACTGC CGTTTTCTGC
     1981 AGGGCCCGGA AACCGATCGT GCGACCGTGC GTAAAATTCG TGATGCGATT GATAACCAGA
     2041 CCGAAGTGAC CGTGCAGCTG ATTAACTATA CCAAAAGCGG CAAAAAATTT TGGAACCTGT
     2101 TTCATCTGCA GCCGATGCGT GATCAGAAAG GCGATGTGCA GTATTTTATT GGCGTGCAGC
     2161 TGGATGGTAC CGAAcgtGTG CGTGATGCGG CGGAACGTGA AGCTGTGATG CTGgtcAAGA
     2221 AAACCGCGga gGAAatcgac gaagccgcta agGAAAATCT CTACTTCCAG ATGGGCGGAg
     2281 gatccGACTA CAAGGATGAC GACGATAAGa tgtctagatt agataaaagt aaagtgatta
     2341 acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg
     2401 cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt
     2461 tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag
     2521 aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac
     2581 taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg
     2641 aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat
     2701 tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc
     2761 atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac
     2821 gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg
     2881 aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gcgtacagcc
     2941 gcgcgcgtac gaaaaacaat tacgggtcta ccatcgaggg cctgctcgat ctcccggacg
     3001 acgacgcccc cgaagaggcg gggctggcgg ctccgcgcct gtcctttctc cccgcgggac
     3061 acacgcgcag actgtcgacg gcccccccga ccgatgtcag cctgggggac gagctccact
     3121 tagacggcga ggacgtggcg atggcgcatg ccgacgcgct agacgatttc gatctggaca
     3181 tgttggggga cggggattcc ccgggtccgg gatttacccc ccacgactcc gccccctacg
     3241 gcgctctgga tatggccgac ttcgagtttg agcagatgtt taccgatgcc cttggaattg
     3301 acgagtacgg tgggTAAtag GAATTCctcg agCTAGAGCT CGCTGATCAG CCTCGACTGT
     3361 GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA
     3421 AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG
     3481 TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA
     3541 AGACAATAGC AGGCATGCTG GGGAggtaac cacgtgcgga ccgagcggcc gcaggaaccc
     3601 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     3661 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     3721 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtattcac
     3781 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     3841 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3901 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3961 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4021 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4081 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4141 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4201 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     4261 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     4321 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     4381 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     4441 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     4501 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4561 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4621 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4681 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4741 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4801 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4861 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4921 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4981 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5041 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5101 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5161 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     5221 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5281 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5341 taaatctgga gccggtgagc gtggaagccg cggtatcatt gcagcactgg ggccagatgg
     5401 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5461 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5521 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5581 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5641 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5701 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5761 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5821 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5881 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5941 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6001 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6061 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6121 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6181 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6241 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6301 ggccttttgc tggccttttg ctcacatgt
//