LOCUS       40_AAV_Syn_BArr2-TEVp-V 5618 bp ds-DNA     circular     22-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /label="misc_feature"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    151..628
                     /label="syn"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    651..1877
                     /label="Arrestin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             651..1877
                     /label="Translation 651-1877"
     misc_feature    1878..1907
                     /label="misc_feature"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1878..1907
                     /label="Translation 1878-1907"
     misc_feature    1908..2051
                     /label="TEV for yeast"
                     /ApEinfo_revcolor=#ff51d8
                     /ApEinfo_fwdcolor=#ff51d8
     misc_feature    1908..2090
                     /label="S219V-tev in BL21 DNA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             1908..2543
                     /label="Translation 1908-2543"
     misc_feature    2058..2090
                     /label="TEV for yeast"
                     /ApEinfo_revcolor=#ff51d8
                     /ApEinfo_fwdcolor=#ff51d8
     misc_feature    2091..2542
                     /label="S219V-tev in BL21 DNA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2091..2542
                     /label="misc_feature"
                     /ApEinfo_revcolor=#ff51d8
                     /ApEinfo_fwdcolor=#ff51d8
     misc_feature    2544..2561
                     /label="misc_feature"
                     /ApEinfo_revcolor=#ff51d8
                     /ApEinfo_fwdcolor=#ff51d8
     misc_feature    2544..2561
                     /label="misc_feature"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             2544..2561
                     /label="Translation 2544-2561"
     CDS             2562..2564
                     /label="Translation 2562-2564"
     misc_feature    2565..2606
                     /label="V5"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             2565..2606
                     /label="Translation 2565-2606"
     CDS             2565..2609
                     /label="Translation 2565-2609"
     misc_feature    2610..2615
                     /label="misc_feature"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2622..2853
                     /label="polyA"
                     /ApEinfo_revcolor=#ff2549
                     /ApEinfo_fwdcolor=#ff2549
     misc_feature    2882..3022
                     /label="ITR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtTGTACA gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaaggatc cGCCACCATG GGTGAAAAAC
      661 CCGGGACCAG GGTCTTCAAG AAGTCGAGCC CTAACTGCAA GCTCACCGTG TACTTGGGCA
      721 AGCGTGACTT TGTGGATCAC TTGGACAAAG TGGATCCTGT CGATGGTGTG GTGCTTGTGG
      781 ATCCTGACTA CTTGAAGGAC CGGAAAGTGT TTGTGACCCT CACCTGTGCC TTCCGCTATG
      841 GCCGAGAAGA CCTGGATGTA CTGGGCCTGT CTTTCCGCAA AGATCTGTTC ATCGCCACCT
      901 ACCAGGCCTT CCCCCCCATG CCCAACCCAC CTCGGCCCCC CACCCGCCTA CAGGACCGAC
      961 TGCTGAAGAA GTTGGGCCAG CATGCCCACC CCTTTTTTTT CACAATACCC CAGAATTTGC
     1021 CTTGCTCCGT CACACTGCAG CCAGGACCGG AGGACACAGG GAAGGCCTGT GGAGTAGACT
     1081 TTGAGATTCG AGCCTTCTGT GCCAAATCTA TAGAAGAAAA AAGCCACAAA AGGAACTCCG
     1141 TGCGGCTTAT CATCAGAAAG GTACAGTTTG CTCCTGAGAC ACCCGGCCCC CAGCCATCAG
     1201 CTGAAACCAC ACGCCACTTC CTCATGTCTG ACCGGAGGTC CCTGCACCTA GAGGCTTCCC
     1261 TGGACAAAGA GCTGTACTAC CATGGGGAAC CCCTCAATGT CAACGTCCAC GTCACCAACA
     1321 ATTCTGCCAA GACCGTCAAG AAGATCAGAG TGTCTGTGAG ACAGTATGCC GACATTTGCC
     1381 TCTTCAGCAC CGCGCAGTAC AAGTGTCCTG TGGCTCAGCT TGAACAAGAT GACCAGGTGT
     1441 CTCCCAGTTC CACATTCTGC AAGGTGTACA CCATAACCCC GCTGCTCAGT GACAACCGAG
     1501 AGAAGCGTGG CCTTGCCCTT GATGGGCAAC TCAAGCACGA AGACACCAAC CTGGCTTCCA
     1561 GCACCATTGT GAAGGAGGGA GCCAACAAGG AGGTGCTGGG AATCCTAGTA TCCTACAGGG
     1621 TCAAGGTGAA GCTGGTGGTG TCTCGAGGCG GGGATGTCTC CGTGGAGCTA CCTTTCGTCC
     1681 TAATGCACCC CAAGCCCCAC GACCACATCA CCCTTCCCCG ACCCCAGTCA GCCCCCCGGG
     1741 AAATAGACAT CCCTGTGGAT ACCAACCTCA TTGAATTCGA TACCAACTAT GCCACAGACG
     1801 ACGACATCGT GTTTGAGGAC TTTGCGAGGC TTCGGCTGAA GGGGATGAAG GATGACGACT
     1861 GTGATGACCA GTTCTGCGGT GGAAGTGGAT CAGGCAGCGG TGGATCTGGA GAAAGCttgt
     1921 ttaagggacc acgtgattac aacccgatat cgagcaccat ttgtcatttg acgaatgaat
     1981 ctgatgggca cacaacatcg ttgtatggta ttggatttgg tcccttcatc attacaaaca
     2041 agcacttgtt tAGAAGAaat aatggaacac tgttggtcca atcactacat ggtgtattca
     2101 aggtcaagaa caccacgact ttgcaacaac acctcattga tgggagggac atgataatta
     2161 ttcgcatgcc taaggatttc ccaccatttc ctcaaaagct gaaatttaga gagccacaaa
     2221 gggaagagcg catatgtctt gtgacaacca acttccaaac taagagcatg tctagcatgg
     2281 tgtcagacac tagttgcaca ttcccttcat ctgatggcat attctggaag cattggattc
     2341 aaaccaagga tgggcagtgt ggcagtccat tagtatcaac tagagatggg ttcattgttg
     2401 gtatacactc agcatcgaat ttcaccaaca caaacaatta tttcacaagc gtgccgaaaa
     2461 acttcatgga attgttgaca aatcaggagg cgcagcagtg ggttagtggt tggcgattaa
     2521 atgctgactc agtattgtgg ggtggccata aagttttcat ggtgGGCAAG CCGATCCCTA
     2581 ACCCtCTGCT GGGCCTGGAC AGCACTTAAG AATTCctcga gCTAGAGCTC GCTGATCAGC
     2641 CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT
     2701 GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA
     2761 TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA
     2821 GGATTGGGAA GACAATAGCA GGCATGCTGG GGAggtaacc acgtgcggac cgagcggccg
     2881 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     2941 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     3001 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     3061 ggtattcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     3121 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3181 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3241 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3301 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3361 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3421 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
     3481 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     3541 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     3601 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     3661 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     3721 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     3781 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     3841 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3901 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     3961 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4021 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4081 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4141 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4201 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4261 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4321 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4381 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4441 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4501 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4561 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4621 tattgctgat aaatctggag ccggtgagcg tggaagccgc ggtatcattg cagcactggg
     4681 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4741 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     4801 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4861 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4921 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4981 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5041 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5101 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5161 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5221 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5281 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5341 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5401 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5461 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5521 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5581 acggttcctg gccttttgct ggccttttgc tcacatgt
//