LOCUS       AtU6-26gRNA-pJET        3837 bp    DNA     circular SYN 02-FEB-2022
DEFINITION  Cloning vector pJET1.2, complete sequence.
ACCESSION   EF694056
VERSION     EF694056.1  GI:149999561
KEYWORDS    .
SOURCE      Cloning vector pJET1.2
  ORGANISM  Cloning vector pJET1.2
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Bagdanaviciute,J., Zakareviciene,L. and Lubys,A.
  TITLE     Improved positive selection cloning vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Lubys,A., Bagdanaviciute,J. and Zakareviciene,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2007) Research Center, Fermentas UAB, Graiciuno
            8, Vilnius LT-02241, Lithuania
FEATURES             Location/Qualifiers
     primer_bind     346..365
                     /standard_name="F1"
     regulatory      475..922
                     /note="Geneious type: promoter"
                     /standard_name="AtU6-26 promoter"
     misc_feature    924..943
                     /note="Geneious type: Misc._feature"
                     /standard_name="spacer"
     ncRNA           944..1019
     primer_bind     944..966
                     /standard_name="JFL-F"
     primer_bind     complement(1097..1119)
                     /standard_name="R1"
     primer_bind     complement(1199..1218)
                     /standard_name="R2"
     primer_bind     complement(1292..1319)
                     /standard_name="R3"
     primer_bind     complement(1395..1419)
                     /standard_name="R4"
     primer_bind     complement(1490..1516)
                     /standard_name="R5"
     primer_bind     complement(1600..1624)
                     /standard_name="R6"
     rep_origin      complement(1967..2640)
     CDS             complement(2785..3645)
                     /gene="bla"
                     /function="catalyzes the opening and hydrolysis of the
                     beta-lactam ring of beta-lactam antibiotics such as
                     penicillins and cephalosporins"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="ABR53873.1"
                     /db_xref="GI:149999562"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
ORIGIN      
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 tcagcaagat gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg
      421 ggtaccgagc tcggatccac tagtaacgga cgccagtgtg ctggaattgc ccttaagctt
      481 cgttgaacaa cggaaactcg acttgccttc cgcacaatac atcatttctt cttagctttt
      541 tttcttcttc ttcgttcata cagttttttt ttgtttatca gcttacattt tcttgaaccg
      601 tagctttcgt tttcttcttt ttaactttcc attcggagtt tttgtatctt gtttcatagt
      661 ttgtcccagg attagaatga ttaggcatcg aaccttcaag aatttgattg aataaaacat
      721 cttcattctt aagatatgaa gataatcttc aaaaggcccc tgggaatctg aaagaagaga
      781 agcaggccca tttatatggg aaagaacaat agtatttctt atataggccc atttaagttg
      841 aaaacaatct tcaaaagtcc cacatcgctt agataagaaa acgaagctga gtttatatac
      901 agctagagtc gaagtagtga ttgctcgcac tagtgaaccc caggttttag agctagaaat
      961 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     1021 ttttttgtcc cttcgaaggg caattctgca gatatccatc acactggcgg ccgctagagg
     1081 tcgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc actagttcta
     1141 gaacggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaatttcg
     1201 agcttggcgt aatcatggtc atagctgttt cctgatcttt ctagaagatc tcctacaata
     1261 ttctcagctg ccatggaaaa tcgatgttct tcttttattc tctcaagatt ttcaggctgt
     1321 atattaaaac ttatattaag aactatgcta accacctcat caggaaccgt tgtaggtggc
     1381 gtgggttttc ttggcaatcg actctcatga aaactacgag ctaaatattc aatatgttcc
     1441 tcttgaccaa ctttattctg catttttttt gaacgaggtt tagagcaagc ttcaggaaac
     1501 tgagacagga attttattaa aaatttaaat tttgaagaaa gttcagggtt aatagcatcc
     1561 attttttgct ttgcaagttc ctcagcattc ttaacaaaag acgtctcttt tgacatgttt
     1621 aaagtttaaa cctcctgtgt gaaattgtta tccgctcaca attccacaca ttatacgagc
     1681 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     1741 gttgcgctca ctgccaattg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     1801 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     1861 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1921 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1981 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2041 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2101 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2161 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2221 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2281 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2341 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2401 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2461 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2521 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2581 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2641 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2701 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2761 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2821 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2881 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2941 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3001 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3061 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3121 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3181 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3241 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3301 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3361 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3421 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3481 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3541 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3601 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3661 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3721 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     3781 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggcc
//
