LOCUS       pKD279.8                3875 bp ds-DNA     circular     25-APR-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1019..1123
                     /label="pEXT20 expression vector source"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1218..1223
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1264..1983
                     /label="superfolder GFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3111..3770
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1124..1135
                     /label="Stop region"
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_fwdcolor=#408080
     misc_feature    1159..1166
                     /label="YvcP/PsdR binding site (inverted repeat II)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    890..1018
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     rep_origin      complement(2302..2984)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1147..1154
                     /label="YvcP/PsdR binding site (inverted repeat I)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1996..2124
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1230..1230
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    138..172
                     /label="sRBS (1,000)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    173..562
                     /label="SO_4388"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1191..1196
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      2989..3094
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    323..325
                     /label="D51"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    103..137
                     /label="J23108"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1246..1263
                     /label="BBa_B0034"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1136..1245
                     /label="PpsdA110"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    563..877
                     /label="yvcP/psdR"
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_fwdcolor=#0080c0
ORIGIN
        1 GTCTCATTTT CGCCAGATAT CGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT
       61 AAAAATAGGC GTATCACGAG GCCCTTTCGT CTTCACCTCG AGctgacagc tagctcagtc
      121 ctaggtataa tgctagcTGC CCCGTATAAT AGGTTATAAG ACGGAATTAT TTatgaaaat
      181 tcttatcgcc gaagatgata ttcatatccg ccaagggttg gccgatatgc tttccagaga
      241 gggatattcc gttctgcttg ctgataatgg caaagttgcc ctgttaaaat atcaacaaga
      301 gcagccggat tttattattc tggatattat gatgcctgag ttagatggct attcagtctg
      361 caaagaaatc agaaagcacg atgaacaaac gccggtgatc tttttatccg ctaaaggtga
      421 agagcttgat aaagttctgg ggcttgagct tggtgcggat gactatatta ataaaccatt
      481 tggtattcac gaggttcggg cgcgaatcaa aacaattgcc cgacgctgtc tgaaagccaa
      541 acagaattca ccagatcaaa gcGTGGAGCT TGGCGGGCTG ACGATTTATC CTGATCAGAA
      601 TGAGGCCGAA TGGAACAGCG TCCGGATTTT GTTTTCCCAA AAAGAATTCC AGCTTCTCTC
      661 GATTTTTGTG AGGGAGCATA AAAAAATTGT CTCTCGTGAT GAACTATTGG AGGCGTTATG
      721 GGATGACGTT GATTTTGTCG ATGACAATAC GTTAACGGTC AATGTCAATC GGCTGCGGAG
      781 AAAACTGGAG AATGCAGGAC TGACAGATTG CATTTCCACA ATCAGAGGAC AAGGTTATCA
      841 ATTTCAAGTC AATCGGAAGG ATGAAGCGGA ATGTTAATAA TAATCTAGAC CAGGCATCAA
      901 ATAAAACGAA AGGCTCAGTC GAAAGACTGG GCCTTTCGTT TTATCTGTTG TTTGTCGGTG
      961 AACGCTCTCT ACTAGAGTCA CACTGGCTCA CCTTCGGGTG GGCCTTTCTG CGTTTATAGT
     1021 AAATCACTGC ATAATTCGTG TCGCTCAAGG CGCACTCCCG TTCTGGATAA TGTTTTTTGC
     1081 GCCGACATCA TAACGGTTCT GGCAAATATT CTGAAATGAG CTGGTTAGCT AGTCACACCC
     1141 TCGTGAATGT GACAGCATTG TAAGATTGGG GAGCGGAATT GCAAGAAAGT TCGATGGGAG
     1201 GATGCTGACT TCCTTTTTAT AATAAAGAAA AAGGAGGAGC AGAACAAAGA GGAGAAATAC
     1261 TAGATGCGTA AAGGCGAAGA GCTGTTCACT GGTGTCGTCC CTATTCTGGT GGAACTGGAT
     1321 GGTGATGTCA ACGGTCATAA GTTTTCCGTG CGTGGCGAGG GTGAAGGTGA CGCAACTAAT
     1381 GGTAAACTGA CGCTGAAGTT CATCTGTACT ACTGGTAAAC TGCCGGTACC TTGGCCGACT
     1441 CTGGTAACGA CGCTGACTTA TGGTGTTCAG TGCTTTGCTC GTTATCCGGA CCATATGAAG
     1501 CAGCATGACT TCTTCAAGTC CGCCATGCCG GAAGGCTATG TGCAGGAACG CACGATTTCC
     1561 TTTAAGGATG ACGGCACGTA CAAAACGCGT GCGGAAGTGA AATTTGAAGG CGATACCCTG
     1621 GTAAACCGCA TTGAGCTGAA AGGCATTGAC TTTAAAGAAG ACGGCAATAT CCTGGGCCAT
     1681 AAGCTGGAAT ACAATTTTAA CAGCCACAAT GTTTACATCA CCGCCGATAA ACAAAAAAAT
     1741 GGCATTAAAG CGAATTTTAA AATTCGCCAC AACGTGGAGG ATGGCAGCGT GCAGCTGGCT
     1801 GATCACTACC AGCAAAACAC TCCAATCGGT GATGGTCCTG TTCTGCTGCC AGACAATCAC
     1861 TATCTGAGCA CGCAAAGCGT TCTGTCTAAA GATCCGAACG AGAAACGCGA TCATATGGTT
     1921 CTGCTGGAGT TCGTAACCGC AGCGGGCATC ACGCATGGTA TGGATGAACT GTACAAATGA
     1981 TGATAATAAT CTAGACCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT
     2041 TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCTACTA GAGTCACACT GGCTCACCTT
     2101 CGGGTGGGCC TTTCTGCGTT TATAGGTACC CGTGGATCCT CTAGAGTCGA CCTGCAGGCA
     2161 TGCAAGCTTC CTAGGCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT
     2221 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA
     2281 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC
     2341 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     2401 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     2461 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC
     2521 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     2581 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     2641 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     2701 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     2761 GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     2821 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     2881 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
     2941 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGACTAGTG CTTGGATTCT
     3001 CACCAATAAA AAACGCCCGG CGGCAACCGA GCGTTCTGAA CAAATCCAGA TGGAGTTCTG
     3061 AGGTCATTAC TGGATCTATC AACAGGAGTC CAAGCGAGCT CGATATCAAA TTACGCCCCG
     3121 CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA TTCTGCCGAC ATGGAAGCCA
     3181 TCACAGACGG CATGATGAAC CTGAATCGCC AGCGGCATCA GCACCTTGTC GCCTTGCGTA
     3241 TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT CCATATTGGC CACGTTTAAA
     3301 TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA AAAACATATT CTCAATAAAC
     3361 CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA CATCTTGCGA ATATATGTGT
     3421 AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG ATGAAAACGT TTCAGTTTGC
     3481 TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA TCACCAGCTC ACCGTCTTTC
     3541 ATTGCCATAC GAAATTCCGG ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC
     3601 GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA
     3661 ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA
     3721 TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT TTTTCTCCAT TTTAGCTTCC
     3781 TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT TATTTCATTA
     3841 TGGTGAAAGT TGGAACCTCT TACGTGCCGA TCAAC
//