LOCUS       Exported                2832 bp ds-DNA     circular SYN 14-MAY-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   TRS
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 14, 2021 from SnapGene 5.2.5.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(4..663)
                     /label=AmpR
                     /note="locus_tag=""AmpR"" - label=""AmpR"" - 
                     ApEinfo_label=""AmpR"" - ApEinfo_fwdcolor=""yellow"" - 
                     ApEinfo_revcolor=""yellow"" - 
                     ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0"" - complement(4..663)"
                     /note="color: #ff4500; direction: LEFT"
     misc_feature    862..966
                     /label=AMPR promoter
                     /note="locus_tag=""AMPR promoter"" - label=""AMPR 
                     promoter"" - ApEinfo_label=""AMPR promoter"" - 
                     ApEinfo_fwdcolor=""#00ff00"" - ApEinfo_revcolor=""green"" -
                     ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0"" - 862..966"
                     /note="color: #dc143c; direction: RIGHT"
     promoter        986..1004
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #00ffff; direction: RIGHT"
     protein_bind    1005..1023
                     /gene="tetO"
                     /allele="Tet operator (TetO2 from Tn10). TetR binds here to
                     suppress transcription."
                     /label=TetO
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_feature    1030..1042
                     /label=RBS
                     /note="ribosome binding site"
                     /note="color: #ff00ff"
     misc_feature    1051..1833
                     /label=GFP-ssra [3']
                     /note="locus_tag=""GFP-ssra"" - label=""GFP-ssra"" - 
                     ApEinfo_label=""GFP-ssra"" - ApEinfo_fwdcolor=""#80ff80"" -
                     ApEinfo_revcolor=""#80ff80"" - 
                     ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0"" - 1080..1862"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    1768..1830
                     /label=ssra
                     /note="locus_tag=""ssra"" - label=""ssra"" - 
                     ApEinfo_label=""ssra"" - ApEinfo_fwdcolor=""#ff8000"" - 
                     ApEinfo_revcolor=""#ff8000"" - 
                     ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0"" - 1797..1859"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    1786..1830
                     /label=ssra(1)
                     /note="locus_tag=""ssra(1)"" - label=""ssra(1)"" - 
                     ApEinfo_label=""ssra"" - ApEinfo_fwdcolor=""#ff0080"" - 
                     ApEinfo_revcolor=""#ff0080"" - 
                     ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0"" - 1815..1859"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    1840..1887
                     /label=T7 stop
                     /note="locus_tag=""T7 stop"" - label=""T7 stop"" - 
                     ApEinfo_label=""T7 stop"" - ApEinfo_fwdcolor=""#800000"" - 
                     ApEinfo_revcolor=""#800000"" - 
                     ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0"" - 1869..1916"
                     /note="color: #dc143c; direction: RIGHT"
     rep_origin      complement(2033..2715)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="locus_tag=""ColE1 origin"" - label=""ColE1 origin"" 
                     - ApEinfo_label=""ColE1 origin"" - 
                     ApEinfo_fwdcolor=""gray50"" - ApEinfo_revcolor=""gray50"" -
                     ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0"" - complement(2062..2744)"
                     /note="color: #f08080"
     misc_feature    2727..2821
                     /label=T0
                     /note="locus_tag=""T0"" - label=""T0"" - 
                     ApEinfo_label=""T0"" - ApEinfo_fwdcolor=""#ff8000"" - 
                     ApEinfo_revcolor=""green"" - 
                     ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0"" - 2756..2850"
                     /note="color: #dc143c; direction: RIGHT"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcgct agcggtaccc catggtaata cgactcacta taggtcccta tcagtgatag
     1021 agagtttaac tttaagaagg aggagtacat atggtgagca agggcgagga gctgttcacc
     1081 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1141 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1201 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     1261 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1321 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1381 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1441 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     1501 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     1561 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1621 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     1681 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1741 actctcggca tggacgagct gtacaagtcc ggactcagat ctcgagcggc gaacgacgaa
     1801 aactactctg aaaactacgc ggacgcgtct tagaagcttc tagcataacc ccttggggcc
     1861 tctaaacggg tcttgagggg ttttttgccc gggggatccc ctagggcgtt cggctgcggc
     1921 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1981 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2041 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2101 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2161 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2221 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     2281 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2341 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2401 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2461 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     2521 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2581 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2641 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2701 ggattttggt catgactagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     2761 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     2821 ccaagcgagc tc
//
