LOCUS       ATP1A1_G2_Dual_s        3003 bp    DNA     circular UNA 28-JUN-2021
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local...2o-djqnibz
VERSION     urn.local...2o-djqnibz
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..241
                     /standard_name="Human U6 Promoter"
     misc_feature    268..343
                     /note="chimeric guide RNA scaffold"
                     /note="Geneious type: misc_RNA"
                     /standard_name="sgRNA scaffold"
     misc_feature    344..349
                     /note="U6 terminator"
                     /note="Geneious type: terminator"
                     /standard_name="U6 terminator"
     promoter        448..702
                     /standard_name="U6 promoter"
     misc_feature    711..730
                     /note="Geneious type: misc_RNA"
                     /standard_name="ATP1A1 G2 (19bp)"
     misc_feature    731..806
                     /note="Geneious type: misc_RNA"
                     /standard_name="sgRNA scaffold"
     terminator      807..813
                     /standard_name="U6 Terminator"
     misc_feature    complement(845..866)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="M13R-20"
     misc_feature    complement(1051..1070)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="pUC19 R"
     misc_feature    1234..1821
                     /note="Geneious type: rep_origin"
                     /standard_name="Ori"
     rep_origin      1234
     CDS             complement(1996..2853)
                     /standard_name="AmpR"
     misc_feature    complement(2854..2918)
                     /note="Geneious type: promoter"
                     /standard_name="AmpR promoter"
ORIGIN      
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg ggtcttcgag aagacctgtt ttagagctag aaatagcaag ttaaaataag
      301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg ttttagagct
      361 agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac
      421 aaatggcgtc gactggatcc ggtaccaagg tcgggcagga agagggccta tttcccatga
      481 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga
      541 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
      601 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
      661 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc gatccaagct
      721 gctacagaag gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
      781 ttgaaaaagt ggcaccgagt cggtgctttt tttaagcttg gcgtaatcat ggtcatagct
      841 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      901 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      961 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     1021 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     1081 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1141 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1201 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1261 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1321 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1381 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1441 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1501 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1561 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1621 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     1681 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1741 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1801 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1861 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1921 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1981 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2041 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2101 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2161 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2221 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2281 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2341 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2401 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2461 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2521 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2581 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     2641 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2701 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2761 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2821 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2881 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2941 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg ctagctgtac
     3001 agt
//
