LOCUS       ATP1A1_T804N_hPG        5652 bp    DNA     circular UNA 03-JUL-2021
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local...1u-djqn9vs
VERSION     urn.local...1u-djqn9vs
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             complement(28..1023)
                     /standard_name="PDCD4-TOSI_mScarlet-I_NLS"
     CDS             complement(28..33)
                     /standard_name="2xStop"
     misc_feature    complement(34..81)
                     /note="Geneious type: CDS"
                     /standard_name="NPM1 NLS"
     misc_feature    complement(82..777)
                     /note="Geneious type: CDS"
                     /standard_name="mScarlet-I"
     CDS             complement(778..1023)
                     /standard_name="PDCD4_TOSI"
     misc_feature    complement(796..816)
                     /standard_name="Phosphodegron"
     misc_feature    complement(832..852)
                     /standard_name="NSL"
     misc_feature    complement(1020..1029)
                     /standard_name="Kozak"
     promoter        complement(1048..1563)
                     /standard_name="hPGK1"
     misc_feature    1612..2356
                     /standard_name="ATP1A1_HA-R"
     promoter        2736..2800
                     /standard_name="AmpR promoter"
     CDS             2801..3658
                     /standard_name="AmpR"
     rep_origin      3832..4420
     misc_feature    4584..4603
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="pUC19 R"
     misc_feature    4662..5409
                     /standard_name="ATP1A1_HA-L"
     exon            5223..5377
                     /standard_name="Exon 17"
     misc_feature    5339..5341
                     /standard_name="T804N"
     terminator      complement(5426..5650)
                     /standard_name="bGH Poly A"
ORIGIN      
        1 tgcagggttt aaacgcggcc gctcgagtta tcactttttc ttttttgcct ggccggcctt
       61 tttcgtggcc gccggccttt tcttgtacag ctcgtccatg ccgccggtgg agtggcggcc
      121 ctcggagcgt tcgtactgtt ccaccacggt gtagtcctcg ttgtgggagg tgatgtccaa
      181 cttgcggtcg acgttgtagg cgccgggcat ctgcacgggc ttcttggcct tgtaggtggt
      241 cttgaagtcc gccaggtagc ggccgccgtc cttcaggcgc agggccatct taatgtcgcc
      301 cttcagcacg ccgtcctcgg ggtacaaccg ctcggtggat gcttcccagc ccattgtctt
      361 cttctgcatt acggggccgt caggagggaa gttggtgccg cggagcttca ccttgtagat
      421 cagggtgccg tcctccaggg aggtgtcctg ggtcacggtc acggcgccgc cgtcctcgaa
      481 gttcatcacg cgctcccact tgaagccctc ggggaaggac tgcttatagt agtcggggat
      541 gtcggcgggg tgcttgatga aggccctgga gccgtacatg aactgagggg acaggatgtc
      601 ccaggagaag ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc
      661 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca tggagccctc
      721 catgtgcacc ttgaaccgca tgaactcctt gatcactgcc tcgcccttgc tcaccatgga
      781 gccggagtcg gacacgctat cgcctcttcc agaatctcta gagctgttct tccgcagccg
      841 tcttttggcc ttggcgttga ttctggcctc attgatgctg ctggcgctga tccagttgcc
      901 gttgatctcg ttcttaattt cctcggtgcc ggcattttcc tcgtcgccgc tgaacaggct
      961 gtcgctcagg ttgtcaggat cagcggggtt cacgttcagg atctgctcgt tttccacgtc
     1021 catcgtggcc ttaaggctga gggtacccct ggggagagag gtcggtgatt cggtcaacga
     1081 gggagccgac tgccgacgtg cgctccggag gcttgcagaa tgcggaacac cgcgcgggca
     1141 ggaacagggc ccacactacc gccccacacc ccgcctcccg caccgcccct tcccggccgc
     1201 tgctctcggc gcgccctgct gagcagccgc tattggccac agcccatcgc ggtcggcgcg
     1261 ctgccattgc tccctggcgc tgtccgtctg cgagggtact agtgagacgt gcggcttccg
     1321 tttgtcacgt ccggcacgcc gcgaaccgca aggaaccttc ccgacttagg ggcggagcag
     1381 gaagcgtcgc cggggggccc acaagggtag cggcgaagat ccgggtgacg ctgcgaacgg
     1441 acgtgaagaa tgtgcgagac ccagggtcgg cgccgctgcg tttcccggaa ccacgcccag
     1501 agcagccgcg tccctgcgca aacccagggc tgccttggaa aaggcgcaac cccaaccccg
     1561 tggatgcatt aaaaaacctc ccacacctcc ccctgaacct gaaacaagct tgtgaggtgt
     1621 gagctgtgtc ttcattcact ggcactatgc tcccagcatc catgaggctg gcgttgggaa
     1681 aagaaattct caggaccagt atccagtgtg tgtcccaatc ccggcttcac agaatcagta
     1741 ttaccactct tcaaggcagc agttaacatt tgtttatcag atgggaatct ttatttttgt
     1801 atacttcacc taaaagattt ttaaatggga gggcaagaat tttataacaa aaggttcaca
     1861 atattagctt ccttattttt agtaactaaa ttccttctcc ccaccccttc ccaggttcct
     1921 gccatctccc tggcttatga gcaggctgag agtgacatca tgaagagaca gcccagaaat
     1981 cccaaaacag acaaacttgt gaatgagcgg ctgatcagca tggcctatgg gcagattggt
     2041 aagctgcagc ctggagtggg aagctggcac atctaaggca tctgaggtga tggtgtccac
     2101 ctcaggttga ggttgatttc agagactgca aatccaggcg actttcaggt ctaggatgag
     2161 ccctaacgga gtgagcctgt ggagtttctc tgaactctct tccatgtgag aatacatctg
     2221 ttgctgtttg cccagttacc ttttgtggaa actggttttc tatactgagc accttggaac
     2281 tgggctcaac tctgcctgag aaatgaggat gtccttcctc actgagcctt gcggaacagg
     2341 aggcatggtt ctaagggcta gccggaagcg ctcctccctc tgcgagagca atggctccgt
     2401 ccggtttcga gccatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     2461 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     2521 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     2581 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     2641 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     2701 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2761 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     2821 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     2881 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     2941 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3001 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3061 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3121 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3181 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3241 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3301 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     3361 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3421 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     3481 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     3541 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     3601 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     3661 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     3721 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3781 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3841 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3901 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3961 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4021 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4081 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4141 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4201 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4261 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4321 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4381 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4441 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     4501 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     4561 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     4621 gcctctcccc gcgcgttggc cgattcatta atgcagaatt ccacccacca gagtcattac
     4681 tttgagtagc tcttgttaat gctgggggct atgtttgttg tcacttctca gttctgttat
     4741 ttggtgtagg gcctgtgtga atacttgcct gtgacggttc tcaggcttca taaatagtct
     4801 caataggaaa ggagcagtgt ctgtaatgag tgctcagtgg gggcatgcat cgcactattt
     4861 ccatcgctag gaaaagtgat tggtattaac ccgttttccc ttttctgggg tagggtgcta
     4921 tcgtggctgt gactggtgac ggtgtgaatg actctccagc tttgaagaaa gcagacattg
     4981 gggttgctat ggggattgct ggctcagatg tgtccaagca agctgctgac atgattcttc
     5041 tggatgacaa ctttgcctca attgtgactg gagtagagga aggtgagagc tatttaaggt
     5101 gtacaccaag atcttattca gatactgccc attagcatcc atttctgtat acttcttgga
     5161 tatgttcagt ttccagtgtg cttgtctcat aagctaacag taaaaaatct tggttttcat
     5221 aggtcgtctg atctttgata acttgaagaa atccattgct tataccttaa ccagtaacat
     5281 tcccgagatc accccgttcc tgatatttat tattgcaaac attccactac cactggggaa
     5341 tgtcaccatc ctctgcattg acttgggcac tgacatggtg agtgtcacaa cagtcacaga
     5401 tcgatatcag acgtgatatg tcgacccata gagcccaccg catccccagc atgcctgcta
     5461 ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga
     5521 cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg
     5581 gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta
     5641 gaaggcacag cc
//
