LOCUS       ATP1A1_G4_Q118R_        2706 bp    DNA     circular UNA 28-JUN-2021
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local...47-djqol3k
VERSION     urn.local...47-djqol3k
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..241
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /standard_name="U6 promoter"
     primer_bind     1..21
                     /note="Human U6 promoter, forward primer"
                     /standard_name="hU6-F"
     primer_bind     172..191
                     /note="Human U6 promoter, forward primer"
                     /standard_name="LKO.1 5'"
     misc_RNA        250..269
                     /standard_name="RUNX1 guide"
     misc_RNA        270..345
                     /standard_name="sgRNA scaffold"
     misc_RNA        346..375
                     /standard_name="RUNX1 +4 T to G extension"
     terminator      376..382
                     /standard_name="U6 Terminator"
     rep_origin      complement(479..1067)
     primer_bind     complement(559..578)
                     /note="pBR322 origin, forward primer"
                     /standard_name="pBR322ori-F"
     CDS             complement(1238..2098)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /standard_name="AmpR"
     primer_bind     1861..1880
                     /note="Ampicillin resistance gene, reverse primer"
                     /standard_name="Amp-R"
     promoter        complement(2099..2203)
                     /gene="bla"
                     /standard_name="AmpR promoter"
     promoter        2266..2506
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /standard_name="U6 promoter"
     primer_bind     2266..2286
                     /note="Human U6 promoter, forward primer"
                     /standard_name="hU6-F"
     primer_bind     2437..2456
                     /note="Human U6 promoter, forward primer"
                     /standard_name="LKO.1 5'"
     misc_RNA        2515..2534
                     /standard_name="ATP1A1-G4"
     misc_RNA        2535..2610
                     /standard_name="sgRNA scaffold"
     misc_RNA        2611..2635
                     /standard_name="ATP1A1 Q118R extension"
     misc_RNA        2611..2622
                     /standard_name="RT template (Q118R)"
     misc_difference 2618..2620
                     /standard_name="Q118R (CAA to AGA)"
     misc_RNA        2623..2635
                     /standard_name="PBS"
     terminator      2636..2642
                     /standard_name="U6 Terminator"
ORIGIN      
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg cattttcagg aggaagcgag ttttagagct agaaatagca agttaaaata
      301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgctgtct gaagccctcg
      361 cttcctcctg aaaatttttt ttaagcttgg gccgctcgag gtacctctct acatatgaca
      421 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      481 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      541 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      601 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      661 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      721 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      781 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      841 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      901 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
      961 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1021 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1081 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1141 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1201 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1261 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1321 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     1381 atccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     1441 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     1501 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     1561 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     1621 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     1681 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     1741 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     1801 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     1861 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     1921 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     1981 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2041 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2101 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     2161 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     2221 tgccacctga cgtcgctagc tgtacatacc aaggtcgggc aggaagaggg cctatttccc
     2281 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat
     2341 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     2401 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     2461 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgttcct
     2521 cttctgtagc agctgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     2581 caacttgaaa aagtggcacc gagtcggtgc tagcatcaga gctgctacag aagagttttt
     2641 ttaagcttgg gccgctcgag gtacctctct acgtcgactg gatccggtac caaggtcggg
     2701 caggaa
//
