LOCUS       Exported               12389 bp ds-DNA     circular SYN 19-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 12389)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 29, 2021 from SnapGene 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12389
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             129..989
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(2641..2675)
                     /label=5' TR
     primer_bind     2916..2975
                     /label=JW27 (PE2_FOR)
     primer_bind     2931..2984
                     /label=JW16 (PE2_fwd)
     enhancer        2956..3335
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3336..3539
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate earlypromoter"
     primer_bind     complement(3546..3604)
                     /label=JW31 (TRE_REV)
     primer_bind     complement(3546..3598)
                     /label=JW26 (TRE_rev)
     promoter        3581..3599
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3607..3624
                     /label=JW38 (PE2 F)
     CDS             3613..3669
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian 
                     virus40) large T antigen"
                     /label=SV40 NLS
                     /translation="MKRTADGSEFESPKKKRKV"
     CDS             3670..7770
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA 
                     dueto the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(5699..5731)
                     /label=KAS277 (5'hSpCas9 [AS])
     primer_bind     5706..5736
                     /label=KAS278 (3'hSpCas9 [S])
     primer_bind     7770..7789
                     /label=JW136 linker-M-MLV_F
     CDS             7870..9900
                     /codon_start=1
                     /label=M-MLV Revers Transciptase
                     /label=MMLV RT
                     /translation="TLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQ
                     APLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKP
                     GTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPT
                     SQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLILLQY
                     VDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLT
                     EARKETVMGQPTPKTPRQLREFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLFNWGPDQ
                     QKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKL
                     DPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMT
                     HYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPDLTDQPLPDA
                     DHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAELIALTQALKMAEGK
                     KLNVYTDSRYAFATAHIHGEIYRRRGWLTSEGKEIKNKDEILALLKALFLPKRLSIIHC
                     PGHQKGHSAEARGNRMADQAARKAAITETPDTSTLLIENSSP"
     misc_feature    8467..8469
                     /label=D200N
     misc_feature    8785..8787
                     /label=T306K
     misc_feature    8806..8808
                     /label=W313F
     misc_feature    8839
                     /label=sense mut
     misc_feature    8857..8859
                     /label=T330P
     misc_feature    9676..9678
                     /label=L603W
     CDS             9913..9963
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian 
                     virus40) large T antigen"
                     /label=SV40 NLS
                     /translation="KRTADGSEFEPKKKRKV"
     primer_bind     complement(9941..9963)
                     /label=JW40 (PE2-P2A R)
     primer_bind     complement(9942..9963)
                     /label=JW135 SV40-NLS_R
     primer_bind     complement(9945..9994)
                     /label=JW28 (PE2_REV)
     primer_bind     complement(9945..9986)
                     /label=JW17 (PE2_rev)
     primer_bind     complement(9948..9966)
                     /label=JW39 (PE2 R)
     primer_bind     9994..10014
                     /label=JW20 (bGH-poly(A)-stop_fwd)
     primer_bind     9994..10014
                     /label=JW24 (bGH-poly(A)_fwd)
     misc_feature    9995..10219
                     /label=polyA
     primer_bind     complement(10201..10219)
                     /label=JW21 (bGH-poly(A)_rev)
     primer_bind     10204..10246
                     /label=JW29 (PGK-Blast-rtTA_FOR)
     primer_bind     10209..10246
                     /label=JW18 (PGK-Blast_fwd)
     primer_bind     10209..10246
                     /label=JW22 (PGK-Blast-T2A-rtTA_fwd)
     promoter        10229..10739
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             10749..11147
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             10749..11144
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(11122..11157)
                     /label=JW19 (PGK-Blast-stop_rev)
     primer_bind     11138..11167
                     /label=JW20 (bGH-poly(A)-stop_fwd)
     primer_bind     11142..11167
                     /label=JW24 (bGH-poly(A)_fwd)
     polyA_signal    11148..11372
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    11249
                     /label=mut
     primer_bind     complement(11354..11397)
                     /label=JW21 (bGH-poly(A)_rev)
     misc_feature    complement(11582..11644)
                     /label=3' TR
     primer_bind     complement(11774..11790)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      11932..12387
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc ccgccgggta actcacgggg tatccatgtc catttctgcg gcatccagcc
     2221 aggatacccg tcctcgctga cgtaatatcc cagcgccgca ccgctgtcat taatctgcac
     2281 accggcacgg cagttccggc tgtcgccggt attgttcggg ttgctgatgc gcttcgggct
     2341 gaccatccgg aactgtgtcc ggaaaagccg cgacgaactg gtatcccagg tggcctgaac
     2401 gaacagttca ccgttaaagg cgtgcatggc cacaccttcc cgaatcatca tggtaaacgt
     2461 gcgttttcgc tcaacgtcaa tgcagcagca gtcatcctcg gcaaactctt tccatgccgc
     2521 ttcaacctcg cgggaaaagg cacgggcttc ttcctccccg atgcccagat agcgccagct
     2581 tgggcgatga ctgagccgga aaaaagaccc gacgatatga tcctgatgca gctagattaa
     2641 ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt gctctctctt
     2701 tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg cttggagctc
     2761 ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata acgaccgcgt
     2821 gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact catacgataa
     2881 ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata ttttcttgtt
     2941 atagatatca agcttgacat tgattattga ctagttatta atagtaatca attacggggt
     3001 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     3061 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     3121 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     3181 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     3241 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     3301 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     3361 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     3421 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     3481 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg
     3541 gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc taatacgact cactataggg
     3601 agagccgcca ccatgaaacg gacagccgac ggaagcgagt tcgagtcacc aaagaagaag
     3661 cggaaagtcg acaagaagta cagcatcggc ctggacatcg gcaccaactc tgtgggctgg
     3721 gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc
     3781 gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca
     3841 gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg
     3901 atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc
     3961 cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc
     4021 ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg
     4081 agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg
     4141 gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac
     4201 agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa
     4261 aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag
     4321 agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc
     4381 ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg
     4441 gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg
     4501 ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac
     4561 gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc
     4621 gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc
     4681 gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc
     4741 tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc
     4801 atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg
     4861 ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag
     4921 ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa
     4981 aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga
     5041 aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc
     5101 gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc
     5161 gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc
     5221 accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc
     5281 ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa
     5341 gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg
     5401 gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg
     5461 aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat
     5521 atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc
     5581 tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc
     5641 tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca
     5701 atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac
     5761 gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat
     5821 agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg
     5881 cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac
     5941 atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc
     6001 gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa
     6061 caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat
     6121 gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg
     6181 gacgctatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc
     6241 agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag
     6301 atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac
     6361 aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag
     6421 agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg
     6481 atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg
     6541 aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc
     6601 aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc
     6661 aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg
     6721 cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc
     6781 tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg
     6841 aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg
     6901 gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc
     6961 gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag
     7021 ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc
     7081 gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag
     7141 agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc
     7201 atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg
     7261 cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc
     7321 gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg
     7381 gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt
     7441 gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag
     7501 agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg
     7561 gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg
     7621 ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc
     7681 accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca
     7741 cggatcgacc tgtctcagct gggaggtgac tctggaggat ctagcggagg atcctctggc
     7801 agcgagacac caggaacaag cgagtcagca acaccagaga gcagtggcgg cagcagcggc
     7861 ggcagcagca ccctaaatat agaagatgag tatcggctac atgagacctc aaaagagcca
     7921 gatgtttctc tagggtccac atggctgtct gattttcctc aggcctgggc ggaaaccggg
     7981 ggcatgggac tggcagttcg ccaagctcct ctgatcatac ctctgaaagc aacctctacc
     8041 cccgtgtcca taaaacaata ccccatgtca caagaagcca gactggggat caagccccac
     8101 atacagagac tgttggacca gggaatactg gtaccctgcc agtccccctg gaacacgccc
     8161 ctgctacccg ttaagaaacc agggactaat gattataggc ctgtccagga tctgagagaa
     8221 gtcaacaagc gggtggaaga catccacccc accgtgccca acccttacaa cctcttgagc
     8281 gggctcccac cgtcccacca gtggtacact gtgcttgatt taaaggatgc ctttttctgc
     8341 ctgagactcc accccaccag tcagcctctc ttcgcctttg agtggagaga tccagagatg
     8401 ggaatctcag gacaattgac ctggaccaga ctcccacagg gtttcaaaaa cagtcccacc
     8461 ctgtttaatg aggcactgca cagagaccta gcagacttcc ggatccagca cccagacttg
     8521 atcctgctac agtacgtgga tgacttactg ctggccgcca cttctgagct agactgccaa
     8581 caaggtactc gggccctgtt acaaacccta gggaacctcg ggtatcgggc ctcggccaag
     8641 aaagcccaaa tttgccagaa acaggtcaag tatctggggt atcttctaaa agagggtcag
     8701 agatggctga ctgaggccag aaaagagact gtgatggggc agcctactcc gaagacccct
     8761 cgacaactaa gggagttcct agggaaggca ggcttctgtc gcctcttcat ccctgggttt
     8821 gcagaaatgg cagcccccct gtaccctctc accaaaccgg ggactctgtt taattggggc
     8881 ccagaccaac aaaaggccta tcaagaaatc aagcaagctc ttctaactgc cccagccctg
     8941 gggttgccag atttgactaa gccctttgaa ctctttgtcg acgagaagca gggctacgcc
     9001 aaaggtgtcc taacgcaaaa actgggacct tggcgtcggc cggtggccta cctgtccaaa
     9061 aagctagacc cagtagcagc tgggtggccc ccttgcctac ggatggtagc agccattgcc
     9121 gtactgacaa aggatgcagg caagctaacc atgggacagc cactagtcat tctggccccc
     9181 catgcagtag aggcactagt caaacaaccc cccgaccgct ggctttccaa cgcccggatg
     9241 actcactatc aggccttgct tttggacacg gaccgggtcc agttcggacc ggtggtagcc
     9301 ctgaacccgg ctacgctgct cccactgcct gaggaagggc tgcaacacaa ctgccttgat
     9361 atcctggccg aagcccacgg aacccgaccc gacctaacgg accagccgct cccagacgcc
     9421 gaccacacct ggtacacgga tggaagcagt ctcttacaag agggacagcg taaggcggga
     9481 gctgcggtga ccaccgagac cgaggtaatc tgggctaaag ccctgccagc cgggacatcc
     9541 gctcagcggg ctgaactgat agcactcacc caggccctaa agatggcaga aggtaagaag
     9601 ctaaatgttt atactgatag ccgttatgct tttgctactg cccatatcca tggagaaata
     9661 tacagaaggc gtgggtggct cacatcagaa ggcaaagaga tcaaaaataa agacgagatc
     9721 ttggccctac taaaagccct ctttctgccc aaaagactta gcataatcca ttgtccagga
     9781 catcaaaagg gacacagcgc cgaggctaga ggcaaccgga tggctgacca agcggcccga
     9841 aaggcagcca tcacagagac tccagacacc tctaccctcc tcatagaaaa ttcatcaccc
     9901 tctggcggct caaaaagaac cgccgacggc agcgaattcg agcccaagaa gaagaggaaa
     9961 gtctaatgta gatcccccgg gggatcagcc tcgactgtgc cttctagttg ccagccatct
    10021 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
    10081 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
    10141 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
    10201 gatgcggtgg gctctatggg cggccgctgg ggttggggtt gcgccttttc caaggcagcc
    10261 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
    10321 accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
    10381 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
    10441 ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag
    10501 acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
    10561 gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
    10621 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
    10681 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagc
    10741 aattcaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
    10801 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
    10861 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
    10921 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
    10981 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
    11041 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
    11101 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaactg tgccttctag
    11161 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
    11221 tcccactgtc ctttcctaat aaaatgagaa aattgcatcg cattgtctga gtaggtgtca
    11281 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
    11341 caggcatgct ggggatgcgg tgggctctat ggtctagaac tagtgccccg ggctgcagga
    11401 attcgataaa agttttgtta ctttatagaa gaaattttga gtttttgttt ttttttaata
    11461 aataaataaa cataaataaa ttgtttgttg aatttattat tagtatgtaa gtgtaaatat
    11521 aataaaactt aatatctatt caaattaata aataaacctc gatatacaga ccgataaaac
    11581 acatgcgtca attttacgca tgattatctt taacgtacgt cacaatatga ttatctttct
    11641 agggttaatc tagctgcgtg ttctgcagcg tgtcgagcat cttcatctgc tccatcacgc
    11701 tgtaaaacac atttgcaccg cgagtctgcc cgtcctccac gggttcaaaa acgtgaatga
    11761 acgaggcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
    11821 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
    11881 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
    11941 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
    12001 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
    12061 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
    12121 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
    12181 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
    12241 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
    12301 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
    12361 ttaacaaaat attaacgctt acaatttag
//
