LOCUS       Exported                3748 bp DNA     circular SYN 22-AUG-2020
DEFINITION  pT7RiboJ_Backbone_FGC in pUCIDT-KAN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 29, 2021 from SnapGene 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="pUCIDT-KAN"
                     /mol_type="other DNA"
     source          544..596
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /label=source:synthetic DNA construct(1)
                     /note="source:synthetic DNA construct;color: #ffc0cb"
     source          1335..1418
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="source:synthetic DNA construct;color: #ffc0cb"
     primer_bind     151..170
                     /label=pRS-marker
     primer_bind     352..375
                     /label=M13 Forward (-41)
                     /note="M13 Forward (-41)"
                     /note="/function=Primer"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="/ApEinfo_reevcolor=#85dae9"
     primer_bind     378..395
                     /label=M13 Forward
     primer_bind     379..395
                     /label=M13 Forward (-20)
                     /note="M13 Forward (-20)"
                     /note="/function=Primer"
     gene            483..1525
                     /function="Reference Gene"
                     /label=pT7RiboJ_Backbone_FGC
                     /note="pT7RiboJ_Backbone_FGC"
     misc_feature    489..528
                     /locus_tag="UNS1"
                     /label=UNS1
                     /note="UNS1;color: #00ffff"
     misc_feature    581..595
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
                     /note="T7 promoter;color: #ff99cc"
     attenuator      601..652
                     /locus_tag="RiboJ"
                     /label=RiboJ
                     /note="RiboJ;color: #993366"
     misc_feature    653..675
                     /locus_tag="hairpin to expose RBS in Voigts"
                     /label=hairpin to expose RBS in Voigts
                     /note="hairpin to expose RBS in Voigts;color: #ff0000"
     misc_feature    687..697
                     /locus_tag="Loop"
                     /label=Loop
                     /note="Loop;color: #ec8513"
     misc_feature    687..697
     RBS             703..725
                     /label=efficient ribosome binding site from bacterioph
                     /label=efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)
                     /note="From pEPQD0KN0025"
     primer_bind     complement(706..735)
                     /label=AN163 vector amp rev
                     /label=AN163\vector\amp\rev
     CDS             complement(832..1155)
                     /label=lacZ-alpha
     primer_bind     837..856
                     /label=pRS-marker
     primer_bind     1050..1072
                     /label=M13/pUC Forward
                     /note="/ApEinfo_reevcolor=#85dae9"
     primer_bind     1064..1081
                     /label=M13 Forward
     primer_bind     1065..1081
                     /label=M13 fwd
     misc_feature    1082..1138
                     /label=MCS
     primer_bind     complement(1151..1167)
                     /label=M13 rev
     primer_bind     complement(1151..1167)
                     /label=M13 Reverse
     primer_bind     complement(1164..1186)
                     /label=M13/pUC Reverse
     protein_bind    1175..1191
                     /label=lac operator
     promoter        complement(1199..1229)
                     /label=lac promoter
     protein_bind    1244..1265
                     /label=CAP binding site
     misc_feature    1357..1404
                     /locus_tag="T7 terminator"
                     /label=T7 terminator
                     /note="T7 terminator;color: #800000"
     misc_feature    1434..1473
                     /locus_tag="UNS2"
                     /label=UNS2
                     /note="UNS2;color: #7bff96"
     misc_feature    1474..1481
                     /locus_tag="mauBI cg/cgcgcg"
                     /label=mauBI cg/cgcgcg
                     /note="mauBI cg/cgcgcg;color: #00ffff"
     misc_feature    1483..1522
                     /locus_tag="UNSX"
                     /label=UNSX
                     /note="UNSX;color: #9882ff"
     primer_bind     complement(1574..1590)
                     /label=M13 Reverse (-27)
                     /note="M13 Reverse (-27)"
                     /note="/function=Primer"
     primer_bind     complement(1587..1609)
                     /label=M13 Reverse (-48)
                     /note="M13 Reverse (-48)"
                     /note="/function=Primer"
     protein_bind    1598..1614
                     /label=lac operator
     promoter        complement(1622..1652)
                     /label=lac promoter
     protein_bind    1667..1688
                     /label=CAP binding site
     rep_origin      1961..2575
                     /label=pMB1 Origin
                     /note="pMB1 Origin"
     gene            complement(2735..3550)
                     /function="Drug Resistance"
                     /label=Kanamycin
                     /note="Kanamycin"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcatcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac gcgatgacga tggatagcga
      421 ttcatcgatg agctgacccg atcgccgccg ccggagggtt gcgtttgaga cgggcgacag
      481 atcttaagca ttactcgcat ccattctcag gctgtctcgt ctcgtctccg gaagacatgc
      541 ttaggagcct gcattaggat cgatctcgat cccgcgaaat taatacgact cactataggg
      601 agctgtcacc ggatgtgctt tccggtctga tgagtccgtg aggacgaaac agcctctaca
      661 aataattttg tttaatacta gacagaaaca gaggagatat gctttgttta actttaagaa
      721 ggagatatac aaatgtgaga ccgtcacagc ttgtctgtaa gcggatgccg ggagcagaca
      781 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
      841 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
      901 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
      961 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     1021 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     1081 tgaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg
     1141 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     1201 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     1261 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctag
     1321 gtctcagctt aggtcgccta ggccgctgag caataactag cataacccct tggggcctct
     1381 aaacgggtct tgaggggttt tttgctgaaa acctcgcttt gtcatgtctt caggctggga
     1441 gttcgtagac ggaaacaaac gcagaatcca agccgcgcgc ggccaggata catagattac
     1501 cacaactccg agcccttcca cccgcatcag ttctggacca gcgagctgtg ctgcgactcg
     1561 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     1621 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     1681 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     1741 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     1801 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1861 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1921 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1981 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2041 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2101 ctgttccgac cctgtcgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2161 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2221 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2281 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2341 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2401 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2461 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2521 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2581 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2641 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2701 tctaaagtat atatgagtaa acttggtctg acagttagaa aaactcatcg agcatcaagt
     2761 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     2821 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     2881 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     2941 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaagagtt
     3001 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     3061 tcgcaccaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
     3121 cgccgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     3181 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     3241 tccctgggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     3301 tggtcggaag aggcataaat tccgtcagcc agtttagcct gaccatctca tctgtaacat
     3361 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     3421 acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     3481 ataaatcagc atccatgttg gaatttaatc gcggcttcaa gcaagacgtt tcccgttgaa
     3541 tatggctcat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     3601 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcacacattt
     3661 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     3721 aataggcgta tcacgaggcc ctttcgtc
//
