LOCUS       Exported                4591 bp DNA     circular SYN 14-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 29, 2021 from SnapGene 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     151..170
                     /label=pRS-marker
     primer_bind     352..375
                     /label=M13 Forward (-41)
                     /note="M13 Forward (-41)"
                     /note="/function=Primer"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="/ApEinfo_reevcolor=#85dae9"
     primer_bind     378..395
                     /label=M13 Forward
     primer_bind     379..395
                     /label=M13 Forward (-20)
                     /note="M13 Forward (-20)"
                     /note="/function=Primer"
     gene            483..731
                     /function="Reference Gene"
                     /label=pT7RiboJ_Backbone_FGC
                     /note="pT7RiboJ_Backbone_FGC"
     misc_feature    489..528
                     /locus_tag="UNS1"
                     /label=UNS1
                     /note="UNS1;color: #00ffff"
     misc_feature    581..595
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
                     /note="T7 promoter;color: #ff99cc"
     attenuator      601..652
                     /locus_tag="RiboJ"
                     /label=RiboJ
                     /note="RiboJ;color: #993366"
     misc_feature    653..675
                     /locus_tag="hairpin to expose RBS in Voigts"
                     /label=hairpin to expose RBS in Voigts
                     /note="hairpin to expose RBS in Voigts;color: #ff0000"
     misc_feature    687..697
     misc_feature    687..697
                     /locus_tag="Loop"
                     /label=Loop
                     /note="Loop;color: #ec8513"
     RBS             703..725
                     /label=efficient ribosome binding site from bacterioph
                     /label=efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)
                     /note="From pEPQD0KN0025"
     primer_bind     complement(706..731)
                     /label=AN163 vector amp rev
                     /label=AN163\vector\amp\rev
     CDS             733..2169
                     /codon_start=1
                     /label=RRvT
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYNVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYKGSTGSGSSGPMVSKGEEAIKEFMRFKVSMEGSMNGHEFEIEGEGEGR
                     PYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFRWER
                     VMNFEDGGLVTVTQDSSIQDGTLIYKVKVRGTNFPPDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTVVEQ
                     YERSEGRHHLFLYGMDELYK"
     primer_bind     complement(866..884)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     gene            2170..2368
                     /function="Reference Gene"
                     /label=pT7RiboJ_Backbone_FGC
                     /note="pT7RiboJ_Backbone_FGC"
     misc_feature    2200..2247
                     /locus_tag="T7 terminator"
                     /label=T7 terminator
                     /note="T7 terminator;color: #800000"
     misc_feature    2277..2316
                     /locus_tag="UNS2"
                     /label=UNS2
                     /note="UNS2;color: #7bff96"
     misc_feature    2317..2324
                     /locus_tag="mauBI cg/cgcgcg"
                     /label=mauBI cg/cgcgcg
                     /note="mauBI cg/cgcgcg;color: #00ffff"
     misc_feature    2326..2365
                     /locus_tag="UNSX"
                     /label=UNSX
                     /note="UNSX;color: #9882ff"
     primer_bind     complement(2417..2433)
                     /label=M13 Reverse (-27)
                     /note="M13 Reverse (-27)"
                     /note="/function=Primer"
     primer_bind     complement(2430..2452)
                     /label=M13 Reverse (-48)
                     /note="M13 Reverse (-48)"
                     /note="/function=Primer"
     protein_bind    2441..2457
                     /label=lac operator
     promoter        complement(2465..2495)
                     /label=lac promoter
     protein_bind    2510..2531
                     /label=CAP binding site
     rep_origin      2804..3418
                     /label=pMB1 Origin
                     /note="pMB1 Origin"
     gene            complement(3578..4393)
                     /function="Drug Resistance"
                     /label=Kanamycin
                     /note="Kanamycin"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcatcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac gcgatgacga tggatagcga
      421 ttcatcgatg agctgacccg atcgccgccg ccggagggtt gcgtttgaga cgggcgacag
      481 atcttaagca ttactcgcat ccattctcag gctgtctcgt ctcgtctccg gaagacatgc
      541 ttaggagcct gcattaggat cgatctcgat cccgcgaaat taatacgact cactataggg
      601 agctgtcacc ggatgtgctt tccggtctga tgagtccgtg aggacgaaac agcctctaca
      661 aataattttg tttaatacta gacagaaaca gaggagatat gctttgttta actttaagaa
      721 ggagatatac aaatggtgag caagggcgag gaggtgatca aggaattcat gaggttcaag
      781 gtgaggatgg agggctccat gaatggacat gagtttgaaa ttgaaggaga gggagaggga
      841 cgcccttatg aaggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
      901 ttcgcctggg acattctgtc ccctcagttt atgtatggat ctaaggctta tgtcaaacat
      961 cctgctgata ttcccgacta caagaagctg agcttccccg agggcttcaa gtgggagagg
     1021 gttatgaact tcgaagatgg aggactggtc acagtcacac aggattcctc cctgcaggat
     1081 ggaacactga tttacaatgt gaagatgagg ggcaccaact ttccacccga cggccccgtg
     1141 atgcaaaaga aaacaatggg atgggaggct tccacagaac gcctgtatcc tcgtgatgga
     1201 gtcctgaaag gagagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
     1261 gagtttaaga ccatttatat ggctaaaaaa cctgtccaac tgcctggata ttattatgtc
     1321 gatacaaaac tggacatcac cagccacaac gaggactaca ccatcgtgga gcagtacgag
     1381 aggagcgagg gccgccatca tctgttcctc tatggaatgg atgaactcta taaaggcagc
     1441 accggcagcg gcagctccgg ccccatggtt tccaaaggag aagaagccat taaagagttt
     1501 atgcgcttca aagtcagcat ggaaggcagc atgaacggcc acgagttcga gatcgagggc
     1561 gagggcgagg gcaggcccta cgagggaaca cagacagcta aactgaaagt cacaaaagga
     1621 ggacctctgc ctttcgcttg ggatatcctg agcccccagt tcatgtacgg cagcaaggcc
     1681 tacgtgaagc accccgccga catccctgat tataaaaaac tgtcctttcc tgaaggattc
     1741 agatgggaac gcgtcatgaa tttcgaggac ggcggcctgg tgaccgtgac ccaggacagc
     1801 agcatccagg acggcaccct gatctataaa gtcaaagtgc gcggaacaaa tttccctcct
     1861 gatggacctg tcatgcagaa aaaaaccatg ggctgggaag ccagcaccga gaggctgtac
     1921 cccagggacg gcgtgctgaa gggcgaaatt catcaggctc tgaaactgaa agatggagga
     1981 cattatctgg tcgaattcaa aacaatctac atggccaaga agcccgtgca gctccccggc
     2041 tactactacg tggacaccaa gctggatatt acatcccata atgaagatta tacagttgtc
     2101 gaacagtatg aacgctccga aggaaggcac cacctctttc tgtacggcat ggacgagctg
     2161 tacaagtaag cttaggtcgc ctaggccgct gagcaataac tagcataacc ccttggggcc
     2221 tctaaacggg tcttgagggg ttttttgctg aaaacctcgc tttgtcatgt cttcaggctg
     2281 ggagttcgta gacggaaaca aacgcagaat ccaagccgcg cgcggccagg atacatagat
     2341 taccacaact ccgagccctt ccacccgcat cagttctgga ccagcgagct gtgctgcgac
     2401 tcgtggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2461 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     2521 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2581 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     2641 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2701 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2761 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2821 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2881 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2941 ctcctgttcc gaccctgtcg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3001 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3061 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3121 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3181 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3241 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3301 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3361 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3421 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3481 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3541 caatctaaag tatatatgag taaacttggt ctgacagtta gaaaaactca tcgagcatca
     3601 agtgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
     3661 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
     3721 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     3781 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaga
     3841 gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     3901 cactcgcacc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc
     3961 gatcgccgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
     4021 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     4081 ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     4141 tgatggtcgg aagaggcata aattccgtca gccagtttag cctgaccatc tcatctgtaa
     4201 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     4261 catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     4321 catataaatc agcatccatg ttggaattta atcgcggctt caagcaagac gtttcccgtt
     4381 gaatatggct cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     4441 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcacaca
     4501 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     4561 aaaaataggc gtatcacgag gccctttcgt c
//
