LOCUS       Exported                3853 bp DNA     circular SYN 09-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Fernando Guzman Chavez
  TITLE     Direct Submission
  JOURNAL   Exported Jun 29, 2021 from SnapGene 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     151..170
                     /label=pRS-marker
     primer_bind     352..375
                     /label=M13 Forward (-41)
                     /note="M13 Forward (-41)"
                     /note="/function=Primer"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="/ApEinfo_reevcolor=#85dae9"
     primer_bind     378..395
                     /label=M13 Forward
     primer_bind     379..395
                     /label=M13 Forward (-20)
                     /note="M13 Forward (-20)"
                     /note="/function=Primer"
     gene            483..731
                     /function="Reference Gene"
                     /label=pT7RiboJ_Backbone_FGC
                     /note="pT7RiboJ_Backbone_FGC"
     misc_feature    489..528
                     /locus_tag="UNS1"
                     /label=UNS1
                     /note="UNS1;color: #00ffff"
     misc_feature    581..595
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
                     /note="T7 promoter;color: #ff99cc"
     attenuator      601..652
                     /locus_tag="RiboJ"
                     /label=RiboJ
                     /note="RiboJ;color: #993366"
     misc_feature    653..675
                     /locus_tag="hairpin to expose RBS in Voigts"
                     /label=hairpin to expose RBS in Voigts
                     /note="hairpin to expose RBS in Voigts;color: #ff0000"
     misc_feature    687..697
     misc_feature    687..697
                     /locus_tag="Loop"
                     /label=Loop
                     /note="Loop;color: #ec8513"
     RBS             703..725
                     /label=efficient ribosome binding site from bacterioph
                     /label=efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)
                     /note="From pEPQD0KN0025"
     primer_bind     complement(706..731)
                     /label=AN163 vector amp rev
                     /label=AN163\vector\amp\rev
     primer_bind     732..758
                     /label=Primer 1
     CDS             733..1431
                     /label=mScarlet-I
     primer_bind     736..758
                     /label=E coli mScarlet part 2 F
     regulatory      736
                     /label=vertebrate consensus sequence for strong initia
                     /label=vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)
     primer_bind     complement(866..884)
                     /label=mCherry-R
     primer_bind     1134..1153
                     /label=mCherry-F
     primer_bind     complement(1406..1443)
                     /label=E coli mScarlet part 2 R
     primer_bind     complement(1406..1435)
                     /label=Primer 2
     primer_bind     complement(1406..1429)
                     /label=E coli mScarlet part 2 R
     gene            1432..1630
                     /function="Reference Gene"
                     /label=pT7RiboJ_Backbone_FGC
                     /note="pT7RiboJ_Backbone_FGC"
     misc_feature    1462..1509
                     /locus_tag="T7 terminator"
                     /label=T7 terminator
                     /note="T7 terminator;color: #800000"
     misc_feature    1539..1578
                     /locus_tag="UNS2"
                     /label=UNS2
                     /note="UNS2;color: #7bff96"
     misc_feature    1579..1586
                     /locus_tag="mauBI cg/cgcgcg"
                     /label=mauBI cg/cgcgcg
                     /note="mauBI cg/cgcgcg;color: #00ffff"
     misc_feature    1588..1627
                     /locus_tag="UNSX"
                     /label=UNSX
                     /note="UNSX;color: #9882ff"
     primer_bind     complement(1679..1695)
                     /label=M13 Reverse (-27)
                     /note="M13 Reverse (-27)"
                     /note="/function=Primer"
     primer_bind     complement(1692..1714)
                     /label=M13 Reverse (-48)
                     /note="M13 Reverse (-48)"
                     /note="/function=Primer"
     protein_bind    1703..1719
                     /label=lac operator
     promoter        complement(1727..1757)
                     /label=lac promoter
     protein_bind    1772..1793
                     /label=CAP binding site
     rep_origin      2066..2680
                     /label=pMB1 Origin
                     /note="pMB1 Origin"
     gene            complement(2840..3655)
                     /function="Drug Resistance"
                     /label=Kanamycin
                     /note="Kanamycin"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcatcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac gcgatgacga tggatagcga
      421 ttcatcgatg agctgacccg atcgccgccg ccggagggtt gcgtttgaga cgggcgacag
      481 atcttaagca ttactcgcat ccattctcag gctgtctcgt ctcgtctccg gaagacatgc
      541 ttaggagcct gcattaggat cgatctcgat cccgcgaaat taatacgact cactataggg
      601 agctgtcacc ggatgtgctt tccggtctga tgagtccgtg aggacgaaac agcctctaca
      661 aataattttg tttaatacta gacagaaaca gaggagatat gctttgttta actttaagaa
      721 ggagatatac aaatggtgag caagggcgag gcagtgatca aggagttcat gcggttcaag
      781 gtgcacatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
      841 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      901 ttctcctggg acatcctgtc ccctcagttc atgtacggct ccagggcctt catcaagcac
      961 cccgccgaca tccccgacta ctataagcag tccttccccg agggcttcaa gtgggagcgc
     1021 gtgatgaact tcgaggacgg cggcgccgtg accgtgaccc aggacacctc cctggaggac
     1081 ggcaccctga tctacaaggt gaagctccgc ggcaccaact tccctcctga cggccccgta
     1141 atgcagaaga agacaatggg ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc
     1201 gtgctgaagg gcgacattaa gatggccctg cgcctgaagg acggcggccg ctacctggcg
     1261 gacttcaaga ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtc
     1321 gaccgcaagt tggacatcac ctcccacaac gaggactaca ccgtggtgga acagtacgaa
     1381 cgctccgagg gccgccactc caccggcggc atggacgagc tgtacaagta agcttaggtc
     1441 gcctaggccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
     1501 ggttttttgc tgaaaacctc gctttgtcat gtcttcaggc tgggagttcg tagacggaaa
     1561 caaacgcaga atccaagccg cgcgcggcca ggatacatag attaccacaa ctccgagccc
     1621 ttccacccgc atcagttctg gaccagcgag ctgtgctgcg actcgtggcg taatcatggt
     1681 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     1741 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     1801 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     1861 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     1921 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     1981 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2041 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2101 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2161 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgt
     2221 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2281 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2341 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2401 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2461 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2521 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2581 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2641 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2701 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2761 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2821 agtaaacttg gtctgacagt tagaaaaact catcgagcat caagtgaaac tgcaatttat
     2881 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     2941 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     3001 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     3061 aatcaccatg agtgacgact gaatccggtg agaatggcaa gagtttatgc atttctttcc
     3121 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca ccaaccaaac
     3181 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgccg ttaaaaggac
     3241 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     3301 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgcag
     3361 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     3421 taaattccgt cagccagttt agcctgacca tctcatctgt aacatcattg gcaacgctac
     3481 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     3541 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     3601 tgttggaatt taatcgcggc ttcaagcaag acgtttcccg ttgaatatgg ctcattttag
     3661 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     3721 cattatggtg aaagttggaa cctcttacgt gccgatcaca catttccccg aaaagtgcca
     3781 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     3841 aggccctttc gtc
//
