LOCUS       mNeon_green_CBP_FLAG_UT 7735 bp ds-DNA     circular     29-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    196..409
                     /label="URA3 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    411..1211
                     /label="URA3"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1814..1962
                     /label="LacZ"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1943..1959
                     /label="M13 F"
                     /ApEinfo_revcolor=#00ffaa
                     /ApEinfo_fwdcolor=#00ffaa
     misc_feature    2001..2042
                     /label="biohub_linker"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2001..2042
                     /label="Translation 2001-2042"
     misc_feature    2043..2750
                     /label="Cn mNeongreen"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(2155..2179)
                     /label="MB158/R"
                     /note="sequence: tcctcgtaaccgtcgttggggttac"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     2763..2770
                     /label="P3-3188-CBP-2XFLAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     complement(2763..2770)
                     /label="P2-3188-CBP-2XFLAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             2763..2840
                     /label="CBP"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             2853..2900
                     /label="2xFLAG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2901..2903
                     /label="Stop"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3231..4062
                     /label="ACT1 Promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3231..4912
                     /label="Nat cassette"
                     /ApEinfo_revcolor=#ff53ff
                     /ApEinfo_fwdcolor=#ff53ff
     CDS             4075..4638
                     /label="NrsR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    4642..4892
                     /label="TRP1 Terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4855..4877
                     /label="MJB285"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4996..5014
                     /label="M13 R"
                     /ApEinfo_revcolor=#00ffba
                     /ApEinfo_fwdcolor=#00ffba
     misc_feature    5387..6006
                     /label="pBR322 ori"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    6161..7021
                     /label="Amp"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    7153..7671
                     /label="CEN6-ARS4 "
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
      241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
      301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
      361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag
      421 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
      481 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
      541 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
      601 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
      661 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
      721 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
      781 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
      841 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
      901 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
      961 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
     1021 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
     1081 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
     1141 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
     1201 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
     1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
     1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
     1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     1441 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     1501 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     1561 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     1621 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     1681 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     1741 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg
     1801 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     1861 ctattacgcc agctggcgaa ggggggatgt gctgcaaggc gattaagttg ggtaacgcca
     1921 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
     1981 tagggcgaat tggagctcca GGTGGCGGAC TCGAAGTCTT GTTCCAAGGT CCTGGATCTG
     2041 GTatggtctc caagggtgag gaggacaaca tggcctccct ccccgccacc cacgagctcc
     2101 acatcttcgg ttccatcaac ggtgtcgact tcgacatggt cggtcagggt accggtaacc
     2161 ccaacgacgg ttacgaggag ctcaacctca agtccaccaa gggtgacctc cagttctccc
     2221 cctggatcct cgtcccccac atcggttacg gtttccacca gtacctcccc taccccgacg
     2281 gtatgtcccc cttccaggcc gccatggtcg acggttccgg ttaccaggtc caccgaacca
     2341 tgcagttcga ggacggtgcc tccctcaccg tcaactaccg atacacctac gagggttccc
     2401 acatcaaggg tgaggcccag gtcaagggta ccggtttccc cgccgacggt cccgtcatga
     2461 ccaactccct caccgccgcc gactggtgtc gatccaagaa gacctacccc aacgacaaga
     2521 ccatcatctc caccttcaag tggtcctaca ccaccggtaa cggtaagcga taccgatcca
     2581 ccgcccgaac cacctacacc ttcgccaagc ccatggccgc caactacctc aagaaccagc
     2641 ccatgtacgt cttccgaaag accgagctca agcactccaa gaccgagctc aacttcaagg
     2701 agtggcagaa ggccttcacc gacgtcatgg gtatggacga gctctacaag gggtcagggt
     2761 caaagagaag atggaaaaag aatttcatag ccgtctcagc agccaaccgc tttaagaaaa
     2821 tctcatcctc cggggcactt gggtcagggt cagattataa agatgacgat gacaaggatt
     2881 ataaagatga cgatgacaag taatctggac atgtcaattg tacactgcct tataatgccc
     2941 catgcacttc cagtattagt catttgaagc gttgaaagat ctcttggcag tgtgatcatg
     3001 gaaacaatcc caaacagcag caaactgaaa acgttctggt atgcaatcca tcccatcgta
     3061 caagctctcc aaatacctct tccaatccaa aaacggttta gatgaaagtc tcgtctgatg
     3121 cacttgttag tgacacccca gaatttgatg atcttcgact cacctcgagc ggccgccagt
     3181 gtgatggata tctgcagaat tcgcccttga atagagtcac ttttgctagg CTGCGAGGAT
     3241 GTGAGCTGGA GAGCGGCGCA CGGGAAGGGG AGGACTCACA TAAGCATGCA GGATTCGAGT
     3301 GGCATGGTGT GCACTGAGTG TATGGTTGTC GGAGGAGAGG ATGATGGTAA CAACAATAGC
     3361 AGCAACGTCA CTCGACGCGC GTCCGGTGTG CCACACGGGG TAACGCCGAG TCGCCGTCAG
     3421 GGTCGCCGAG ACCACTCTCA CAGCGTCACC GTTGGCACCA GCTCAGCTTA CAGCTTCTAT
     3481 CCTCCGCCAG CATCCACATA CATCCCCTAT ACCGCATCCC CCACCCACTG CCCAAGGTGA
     3541 GTCATCTTCC CGCCCCCTTC CCTTGCCCGC CACTCAGTCC TCCATCCTCC ACTAATCCAC
     3601 CTTATCGCAC CCACCGCCTA TCGCACATCC GAGCACAATG CTGGGCCTGC CAGGGGCTGC
     3661 TAGATGGTGC TCTCCCCACG CTGATCTGCA TGCCGGCCAT TGGATCATGG GTGCTAGGTG
     3721 CTGGGTGCTG GATGTTGGAT GCTGGATGCT GGGTGCACGC TTGGTCATTT CCTTCCAGGA
     3781 TTGACGGTCG CCGAGAGGAC GACGTGGCGT TCGACAACGA GGGCCGATAG CACCGCATCG
     3841 CCTCGACCTG CATCCATCTC GCCTTGTCCT TTTGGTGCAA CAATCCATCC GTGCTGGTGC
     3901 CACACGCATA GCTGGAAGAG ATGGATGTGC GTTGAACAGA GCTGCCGTCA GGACTTTTTG
     3961 GTGCACGGAC CCTATTGTCC TCCCCAATCT TCACCGCGTC TCCTAATATG CAGCCTCTTT
     4021 GCTAATTGTC TTTTTCCATT AGTAAACTCG CCCAACATGT CTATGGCGGC CGCCACTCTT
     4081 GACGACACGG CTTACCGGTA CCGCACCAGT GTCCCGGGGG ACGCCGAGGC CATCGAGGCA
     4141 CTGGATGGGT CCTTCACCAC CGACACCGTC TTCCGCGTCA CCGCCACCGG GGACGGCTTC
     4201 ACCCTGCGGG AGGTGCCGGT GGACCCGCCC CTGACCAAGG TGTTCCCCGA CGACGAATCG
     4261 GACGACGAAT CGGACGACGG GGAGGACGGC GACCCGGACT CCCGGACGTT CGTCGCGTAC
     4321 GGGGACGACG GCGACCTGGC GGGCTTCGTG GTCGTCTCGT ACTCCGGCTG GAACCGCCGG
     4381 CTGACCGTCG AGGACATCGA GGTCGCCCCG GAGCACCGGG GGCACGGGGT CGGGCGCGCG
     4441 TTGATGGGGC TCGCGACGGA GTTCGCCCGC GAGCGGGGCG CCGGGCACCT CTGGCTGGAG
     4501 GTCACCAACG TTAACGCACC GGCTATCCAC GCGTACCGGC GGATGGGGTT CACCCTCTGC
     4561 GGCCTGGACA CCGCCCTGTA CGACGGCACC GCCTCGGACG GCGAGCAGGC GCTCTACATG
     4621 AGCATGCCCT GCCCCTAAGA ATTCGTGAAG GCGGTAAGGG GTTAATTTTC CTTAGAGGGT
     4681 GTATATATAC ATATTAGAGA AGTGATACAA TTTTAGCACA CTGCGAATTC GAGACAGACA
     4741 TCGTGTCAAT CATCTTTTTT GACATTATAT GCCCATTAAT CTATCTACAG ACAACAATAC
     4801 CATCCTTCCC ACCCTCAGCA ACGCCGTTGA ATCCTCAGGA TCTTCATGGC TCCTTGTCTC
     4861 TGAAACCAGG AAGCTAGTTT CTACATCTCT TCCGTGTTAA TACAGATAAA CCatcgatac
     4921 cgtcgacctc gagggggggc ccggtaccca gcttttgttc cctttagtga gggttaattc
     4981 cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     5041 ttccacacaa cataggagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     5101 ggtaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     5161 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     5221 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5281 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5341 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5401 ttttccatag gctcggcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5461 ggcgaaaccc gacaggacta taaagatacc aggcgttccc ccctggaagc tccctcgtgc
     5521 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5581 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5641 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5701 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5761 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5821 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     5881 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5941 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6001 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6061 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6121 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6181 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctgcccgtcg
     6241 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6301 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6361 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6421 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6481 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6541 caaggcgagt tacatgatcc cccatgttgt gaaaaaaagc ggttagctcc ttcggtcctc
     6601 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6661 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6721 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     6781 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     6841 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     6901 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     6961 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7021 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7081 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7141 aagtgccacc tgggtccttt tcatcacgtg ctataaaaat aattataatt taaatttttt
     7201 aatataaata tataaattaa aaatagaaag taaaaaaaga aattaaagaa aaaatagttt
     7261 ttgttttccg aagatgtaaa agactctagg gggatcgcca acaaatacta ccttttatct
     7321 tgctcttcct gctctcaggt attaatgccg aattgtttca tcttgtctgt gtagaagacc
     7381 acacacgaaa atcctgtgat tttacatttt acttatcgtt aatcgaatgt atatctattt
     7441 aatctgcttt tcttgtctaa taaatatata tgtaaagtac gctttttgtt gaaatttttt
     7501 aaacctttgt ttattttttt ttcttcattc cgtaactctt ctaccttctt tatttacttt
     7561 ctaaaatcca aatacaaaac ataaaaataa ataaacacag agtaaattcc caaattattc
     7621 catcattaaa agatacgagg cgcgtgtaag ttacaggcaa gcgatccgtc ctaagaaacc
     7681 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//