LOCUS       Exported                5614 bp ds-DNA     circular SYN 15-JUL-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 15, 2021 from SnapGene 4.0.8
            http://www.snapgene.com
COMMENT     pFA6a-mCherry-sfGFP-KanMX
            
            mCherry cut out of pMM151 via SalI/XhoI and cloned into SalI cut 
            pMaM4-1
            control digest with SalI/BglII, sequenced from both sides with 
            pYM-seq + His&Kan-Tag 2
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    143..160
                     /label=S1/S3-Site
                     /note="/ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#ff8000"
                     /note="color: #ff8000"
     CDS             161..868
                     /codon_start=1
                     /label=mCherry
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             905..1618
                     /codon_start=1
                     /label=superfolder GFP
                     /note="/ApEinfo_fwdcolor=#80ff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#80ff80"
                     /note="color: #80ff80"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSRLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    1634..1836
                     /label=ADH1-Terminator
                     /note="/ApEinfo_fwdcolor=#0000ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#0000ff"
                     /note="color: #0000ff"
     misc_feature    1874..2252
                     /label=TEF promoter
                     /note="/ApEinfo_fwdcolor=#b90746
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#b90746"
                     /note="color: #b90746"
     primer_bind     complement(1938..1956)
                     /label=His&Kan-Tag 2
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    2253..3062
                     /label=KanMx
                     /note="/ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#ffff00"
                     /note="color: #ffff00; direction: RIGHT"
     misc_feature    3303..3321
                     /label=S2-Site
                     /note="/ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#ff8000"
                     /note="color: #ff8000"
     primer_bind     complement(3369..3388)
                     /label=T7
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
                     /note="color: #00ff00; direction: LEFT"
     rep_origin      complement(3628..4310)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="/ApEinfo_fwdcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=gray50"
                     /note="color: #808080"
     misc_feature    complement(4405..4716)
                     /label=beta-lactamase, C-terminus region
                     /note="/ApEinfo_fwdcolor=#ffff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#ffff80"
                     /note="color: #ffff80; direction: LEFT"
     CDS             complement(4408..5067)
                     /label=AmpR
                     /note="/ApEinfo_fwdcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=yellow"
                     /note="color: #ffff00; direction: LEFT"
ORIGIN
        1 gcggcatcag agcagattgt actgagagtg caccatatgg acatattgtc gttagaacgc
       61 ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg acactataga
      121 acgcggccgc cagctgaagc ttcgtacgct gcaggtcgac atggtgagca agggcgagga
      181 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
      241 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
      301 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
      361 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
      421 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
      481 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
      541 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
      601 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
      661 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
      721 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
      781 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
      841 caccggcggc atggacgagc tgtacaagct cgacggagca ggtgctggtg ctggtgctgg
      901 agcaatgtcc aagggtgaag agctatttac tggggttgta cccattttgg tagaactgga
      961 cggagatgta aacggacata aattctctgt tagaggtgag ggcgaaggcg atgccaccaa
     1021 tggtaaattg actctgaagt ttatatgcac tacgggtaaa ttacctgttc cttggccaac
     1081 cctagtaaca actttgacat atggtgttca atgtttctca agatacccag accatatgaa
     1141 aaggcatgat ttctttaaaa gtgctatgcc agaaggctac gtgcaagaga gaactatctc
     1201 ctttaaggat gacggtacgt ataaaacacg agcagaagtg aaattcgaag gggatacact
     1261 agttaatcgc atcgaattaa agggtataga ctttaaggaa gatggtaata ttctcggcca
     1321 taaacttgag tataatttca actcgcataa tgtgtacatt acagctgaca aacaaaagaa
     1381 cggaattaaa gcgaatttta aaatcaggca caacgtcgaa gatgggtctg ttcaacttgc
     1441 cgatcattat cagcaaaaca cccctattgg tgatggtcca gtcttgttac ccgataatca
     1501 ctacttaagc acacagtcta gattgtcaaa agatccgaat gaaaagcgtg atcacatggt
     1561 tttattggaa tttgtcaccg ctgcaggaat aactcacgga atggacgagc tttataaggg
     1621 atcctaagcg cgccacttct aaataagcga atttcttatg atttatgatt tttattatta
     1681 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac
     1741 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt
     1801 atgaggtcgc tcttattgac cacacctcta ccggcagatc cgctagggat aacagggtaa
     1861 tatagatctg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc
     1921 cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc
     1981 ccgtacattt agcccataca tccccatgta taatcatttg catccataca ttttgatggc
     2041 cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga cctgcgagca gggaaacgct
     2101 cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt
     2161 taggatttgc cactgaggtt cttctttcat atacttcctt ttaaaatctt gctaggatac
     2221 agttctcaca tcacatccga acataaacaa ccatgggtaa ggaaaagact cacgtttcga
     2281 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     2341 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
     2401 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     2461 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     2521 atgatgcatg gttactcacc actgcgatcc ccggcaaaac agcattccag gtattagaag
     2581 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     2641 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     2701 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     2761 gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt
     2821 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     2881 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     2941 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     3001 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     3061 aatcagtact gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt
     3121 ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct
     3181 cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg
     3241 tatgtgaatg ctggtcgcta tactgctgtc gattcgatac taacgccgcc atccagttta
     3301 aacgagctcg aattcatcga tgatatcaga tccactagtg gcctatgcgg ccgcggatct
     3361 gccggtctcc ctatagtgag tcgtattaat ttcgataagc caggttaacc tgcattaatg
     3421 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3481 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3541 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3601 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3661 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3721 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3781 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3841 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3901 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3961 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4021 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4081 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4141 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4201 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4261 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4321 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4381 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4441 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4501 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4561 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4621 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4681 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4741 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4801 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4861 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4921 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4981 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5041 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5101 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5161 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5221 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5281 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5341 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     5401 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     5461 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     5521 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     5581 gggtgttggc gggtgtcggg gctggcttaa ctat
//
