LOCUS       Exported                5596 bp ds-DNA     circular SYN 01-OCT-2020
DEFINITION  New_Assembly 5596bp ds-DNA circular SYN 1-OCT-2020.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 15, 2021 from SnapGene 4.0.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    143..160
                     /label=S1/S3-Site
                     /note="color: #ff6600"
     CDS             161..856
                     /codon_start=1
                     /label=mScarlet-H
                     /note="color: #ff0000"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     HALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     misc_feature    857..892
                     /label=Linker
                     /note="color: #a6acb3"
     CDS             893..1600
                     /codon_start=1
                     /label=mNeonGreen
                     /note="color: #00ff00"
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     terminator      1616..1818
                     /label=ADH1-Terminator
                     /note="color: #0000ff"
     misc_feature    1856..2234
                     /label=TEF promoter
                     /note="color: #800000"
     misc_feature    2235..3044
                     /label=KanMx
                     /note="color: #ffff00"
     misc_feature    3285..3303
                     /label=S2-Site
                     /note="color: #ff6600"
     misc_feature    complement(3610..4292)
                     /label=ColE1 origin
                     /note="color: #a6acb3; direction: LEFT"
     CDS             4390..5049
                     /codon_start=1
                     /label=AmpR
                     /note="color: #ffff00"
                     /translation="PMLNQ*GTYLSDLSISFIHSCLTPRRVDNYDTGGLTIWPQCCNDT
                     ARPTLTGSRFISNKPASRKGRAQKWSCNFIRLHPVY*LLPGS*SK*FAS**FAQRCCHC
                     YRHRGVTLVVWYGFIQLRFPTIKASYMIPHVVQKSG*LLRSSDRCQK*VGRSVITHGYG
                     STA*FSYCHAIRKMLFCDW*VLNQVILRIVYAATELLLPGVNTG*YRAT*QNFKSAH"
ORIGIN
        1 gcggcatcag agcagattgt actgagagtg caccatatgg acatattgtc gttagaacgc
       61 ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg acactataga
      121 acgcggccgc cagctgaagc ttcgtacgct gcaggtcgac atggtgagca agggcgaggc
      181 agtgatcaag gagttcatgc ggttcaaggt gcacatggag ggctccatga acggccacga
      241 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      301 gaaggtgacc aagggtggcc ccctgccctt ctcctgggac atcctgtccc ctcagttcat
      361 gtacggctcc agggccttca ccaagcaccc cgccgacatc cccgactact ataagcagtc
      421 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgccgtgac
      481 cgtgacccag gacacctccc tggaggacgg caccctgatc tacaaggtga agctccgcgg
      541 caccaacttc cctcctgacg gccccgtaat gcagaagaag acaatgggct gggaagcgtc
      601 caccgagcgg ttgtaccccg aggacggcgt gctgaagggc gacattaagc acgccctgcg
      661 cctgaaggac ggcggccgct acctggcgga cttcaagacc acctacaagg ccaagaagcc
      721 cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg gacatcacct cccacaacga
      781 ggactacacc gtggtggaac agtacgaacg ctccgagggc cgccactcca ccggcggcat
      841 ggacgagctg tacaagctcg acggagcagg tgctggtgct ggtgctggag caatggtttc
      901 taagggtgaa gaagacaaca tggcttcttt gccagctact cacgaattgc acatcttcgg
      961 ttctatcaac ggtgttgact tcgacatggt tggtcaaggt actggtaacc caaacgacgg
     1021 ttacgaagaa ttgaacttga agtctactaa gggtgacttg caattctctc catggatctt
     1081 ggttccacac atcggttacg gtttccacca atacttgcca tacccagacg gtatgtctcc
     1141 attccaagct gctatggttg acggttctgg ttaccaagtt cacagaacta tgcaattcga
     1201 agacggtgct tctttgactg ttaactacag atacacttac gaaggttctc acatcaaggg
     1261 tgaagctcaa gttaagggta ctggtttccc agctgacggt ccagttatga ctaactcttt
     1321 gactgctgct gactggtgta gatctaagaa gacttaccca aacgacaaga ctatcatctc
     1381 tactttcaag tggtcttaca ctactggtaa cggtaagaga tacagatcta ctgctagaac
     1441 tacttacact ttcgctaagc caatggctgc taactacttg aagaaccaac caatgtacgt
     1501 tttcagaaag actgaattga agcactctaa gactgaattg aacttcaagg aatggcaaaa
     1561 ggctttcact gacgttatgg gtatggacga attgtacaag ggatcctaag cgcgccactt
     1621 ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa
     1681 taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga
     1741 gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc gctcttattg
     1801 accacacctc taccggcaga tccgctaggg ataacagggt aatatagatc tgtttagctt
     1861 gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac
     1921 agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata
     1981 catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa
     2041 aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg
     2101 aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg
     2161 ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc
     2221 gaacataaac aaccatgggt aaggaaaaga ctcacgtttc gaggccgcga ttaaattcca
     2281 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     2341 cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     2401 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     2461 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     2521 ccactgcgat ccccggcaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     2581 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     2641 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     2701 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     2761 tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     2821 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     2881 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     2941 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     3001 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagta ctgacaataa
     3061 aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg tagttgttct
     3121 attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca tctgcccaga
     3181 tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc
     3241 tatactgctg tcgattcgat actaacgccg ccatccagtt taaacgagct cgaattcatc
     3301 gatgatatca gatccactag tggcctatgc ggccgcggat ctgccggtct ccctatagtg
     3361 agtcgtatta atttcgataa gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg
     3421 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3481 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3541 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3601 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3661 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3721 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3781 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
     3841 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3901 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3961 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4021 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     4081 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4141 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4201 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4261 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4321 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4381 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4441 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4501 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4561 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4621 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4681 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4741 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4801 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4861 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4921 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4981 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     5041 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     5101 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     5161 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     5221 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     5281 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     5341 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     5401 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     5461 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     5521 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     5581 gggctggctt aactat
//
