LOCUS       Exported                3033 bp DNA     circular SYN 08-JUL-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 8, 2021 from SnapGene 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(27..46)
                     /label=HCN-bk-R
                     /note="color: black; sequence: aggtggcacttttcggggaa; added:
                     2020-01-04"
     primer_bind     28..47
                     /label=rrn-F1-F
                     /note="color: black; sequence: 
                     cagtttactttgcagggcttccctccccgaaaagtgccacctG; added: 
                     2019-03-28"
     promoter        53..213
                     /label=rrnB-P1
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    83..152
                     /label=FIS 123
                     /note="color: #a6acb3"
     misc_feature    154..176
                     /label=UP element
                     /note="color: #a6acb3"
     misc_feature    177..182
                     /label=-35
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    199..204
                     /label=-10
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(203..226)
                     /label=rrnM-R
                     /note="color: black; sequence: ATCGGTgaatgtttggtggcgcat; 
                     added: 2020-02-17"
     primer_bind     208..247
                     /label=rrnM-F
                     /note="color: black; sequence: 
                     ccaccaAacattcACCGATATCCGAACGGAGGTAGTTATG; added: 
                     2020-02-17"
     misc_feature    213..244
                     /label=AAAC RBS7k
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    213
                     /label=+1
                     /note="color: #a6acb3"
     misc_feature    245..958
                     /label=GFP-mut2
                     /note="fast-folding protein"
                     /note="color: #00ff00; direction: RIGHT"
     misc_difference 953..955
                     /label=mutation
                     /note="it was TAT (Tyr), found mutated to CAT (His)"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    965..1000
                     /label=LAA tag
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     1017..1036
                     /label=HCN-bk-F
                     /note="color: black; sequence: atggtacgcgtGCTAGAggc; added:
                     2020-01-04"
     terminator      1039..1125
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff"
     primer_bind     complement(1217..1246)
                     /label=rrn-F1-R
                     /note="color: black; sequence: 
                     ctcccgtggggaaaaaatcatggcgctcacatgttctttcctgcg; added: 
                     2019-03-28"
     primer_bind     complement(1217..1237)
                     /label=rrn-F1-R
                     /note="color: black; sequence: 
                     ctcccgtggggaaaaaatcatggcgctcacatgttctttcctgcg; added: 
                     2019-03-28"
     rep_origin      complement(1289..1877)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      1965..2059
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
                     /note="color: #ffffff"
     CDS             complement(2089..2949)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 2089 .. 2880 / #ccffcc
                        2: 2881 .. 2949 / #ccffcc / signal sequence
                     Cleavage site after base 2880"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(join(2950..3033,1..21))
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgccaggag
       61 ctgaacaatt attgcccgtt ttacagcgtt acggcttcga aacgctcgaa aaactggcag
      121 ttttaggctg atttggttga atgttgcgcg gtcagaaaat tattttaaat ttcctcttgt
      181 caggccggaa taactcccta taatgcgcca ccaaacattc accgatatcc gaacggaggt
      241 agttatgtcg aaaggtgagg agttattcac aggggtcgtg cccattttag tggaacttga
      301 cggagatgtc aacggtcaca agtttagtgt gtctggcgag ggggaggggg atgccacgta
      361 tggcaagttg accttgaagt ttatctgtac taccggaaaa ttacccgtgc cgtggccgac
      421 attggtcaca acttttgctt acggtcttca gtgcttcgct cgctaccccg atcatatgaa
      481 gcagcatgat tttttcaaaa gcgcgatgcc cgaggggtat gttcaagaac gtaccatttt
      541 cttcaaggac gatggcaact acaagactcg tgcagaggtc aaatttgagg gtgacactct
      601 tgtaaatcgc attgagttaa agggtatcga tttcaaagaa gacggcaata ttcttgggca
      661 caaattagaa tacaactaca acagccataa cgtgtatatt atggctgaca aacaaaagaa
      721 tggaattaag gttaatttta agatccgcca taatattgag gacggatcag ttcagctggc
      781 ggatcactat caacagaata caccgatcgg tgacggccct gtgctgcttc cagacaacca
      841 ctatttatcc actcagtcca agttatctaa agaccccaat gaaaaacgcg atcatatggt
      901 tttgttagag ttcgtaacgg ctgcaggaat cactcatggt atggacgagt tgcataaaag
      961 gcctgcagca aacgacgaaa actacgcttt agcagcttaa taacccgggg gatcccatgg
     1021 tacgcgtgct agaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1081 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gccctagacc
     1141 taggcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1201 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1261 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1321 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1381 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1441 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     1501 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1561 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1621 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1681 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     1741 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1801 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1861 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1921 acgaaaactc acgttaaggg attttggtca tgactagtgc ttggattctc accaataaaa
     1981 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     2041 ggatctatca acaggagtcc aagcgagctc gtaaacttgg tctgacagtt accaatgctt
     2101 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2161 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2221 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2281 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2341 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2401 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2461 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2521 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2581 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2641 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2701 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2761 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2821 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2881 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     2941 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3001 gagcggatac atatttgaat gtatttagaa aaa
//
