LOCUS       Exported                8825 bp ds-DNA     circular SYN 17-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...g-cf34kig
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 2, 2021 from SnapGene 5.2.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8825
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     4..20
                     /label=M13 rev
     misc_feature    complement(61..196)
                     /label=PiggyBac 5'TR
     misc_feature    77..95
                     /label=TR2
     primer_bind     complement(356..379)
                     /label=Rv2 - PiggyBac NO attP NO lox EM835
     misc_feature    complement(371..591)
                     /label=attP'
     protein_bind    427..526
                     /label=phage phi-C31 attP
     protein_bind    602..635
                     /label=loxP
     misc_feature    638..640
                     /label=lac promoter
     promoter        645..2559
                     /label=Nix promoter
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    645..668
                     /label=Nix Fwd4
                     /note="/created_by=User"
     exon            2560..3305
                     /label=Exon 1
                     /note="/modified_by=User"
     misc_feature    2560..2631
                     /label=5' UTR
     misc_feature    2632..2634
                     /label=Start
     misc_feature    complement(3280..3305)
                     /label=Aal Nix E1 Rvs
     intron          3306..3412
                     /label=Intron1
                     /note="/created_by=User"
     exon            3413..3587
                     /label=Exon2
                     /note="/created_by=User"
     misc_feature    3587^3588
                     /label=GTATGGCAGAATTGAAAAAAATAGACTAGTGTACGACTCAACCGGAC
                     /label=GTATGGCAGAATTGAAAAAAATAGACTAGTGTACGACTCAACCGGACACT 
                     CTAAACAATACGGTTTTGTTTATTATGAAAAGCACTTGTCTGCTCAAGCGGCCAAACA 
                     GGAAATGGACCGCAGTGATCATACAGGACGTAAAATTGCACTGCGGACCGTTCAAGAA 
                     ATTTTGGCAGCGGAAACATTTCTTGACCTTTTCCACAATACTTATCAGCTTTGGAAAA 
                     TCCCCAGAGGTAGATCAATCCTACTCCGCCAAAAACTTCCCTGGCGATGGAAGCTCTT 
                     CCGAACCGATCTTCCCGAGTCGGTGTCGAGCTAGATTCTGTTTTTTCCGGTTGGAGAC 
                     TGGTCTCCGGTTCACTGGCGCCCCAGCGACCGTGCTTTCCATGACGTTATCGATCTAG 
                     CCTACTCTCCCGGCGGTGGACCTAGCCTACTCTGATGGAAAAAATCCCCAGCGAAAGA 
                     AGTTCTCCCGAAACCGAGAATTCAATCTCGACCATCCAGGTGCCGAGGTCGACCCAGC 
                     GATGCCGGTTGGAGGTTGACTTCCAGTTCACTATCACTCCGATTAAG
                     /note="Geneious type: Editing History Deletion"
                     /note="/Original_Bases=GTATGGCAGAATTGAAAAAAATAGACTAGTGTACGA
                     CTCAA 
                     CCGGACACTCTAAACAATACGGTTTTGTTTATTATGAAAAGCACTTGTCTGCTCAAGC 
                     GGCCAAACAGGAAATGGACCGCAGTGATCATACAGGACGTAAAATTGCACTGCGGACC 
                     GTTCAAGAAATTTTGGCAGCGGAAACATTTCTTGACCTTTTCCACAATACTTATCAGC 
                     TTTGGAAAATCCCCAGAGGTAGATCAATCCTACTCCGCCAAAAACTTCCCTGGCGATG 
                     GAAGCTCTTCCGAACCGATCTTCCCGAGTCGGTGTCGAGCTAGATTCTGTTTTTTCCG 
                     GTTGGAGACTGGTCTCCGGTTCACTGGCGCCCCAGCGACCGTGCTTTCCATGACGTTA 
                     TCGATCTAGCCTACTCTCCCGGCGGTGGACCTAGCCTACTCTGATGGAAAAAATCCCC 
                     AGCGAAAGAAGTTCTCCCGAAACCGAGAATTCAATCTCGACCATCCAGGTGCCGAGGT 
                     CGACCCAGCGATGCCGGTTGGAGGTTGACTTCCAGTTCACTATCACTCCGATTAAG"
     terminator      3588..3810
                     /label=SV40 term
                     /note="/modified_by=User"
     primer_bind     3588..3610
                     /label=EM820
     polyA_signal    3691..3773
                     /label=SV40 poly(A) signal
     primer_bind     complement(3789..3819)
                     /label=EM1121
     promoter        3820..5205
                     /label=PUb
                     /note="/created_by=User"
                     /note="/modified_by=User"
     CDS             5206..5922
                     /label=EGFP
     primer_bind     complement(5214..5231)
                     /label=GATC-pEGFP-RP
     primer_bind     complement(5251..5267)
                     /label=GATC-pEGFP_N
     primer_bind     5854..5871
                     /label=GATC-pEGFP_C2-FP
     misc_feature    5923^5924
                     /label=ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCT
                     /label=ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGT 
                     CGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGC 
                     GATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCG 
                     TGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTA 
                     CCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTC 
                     CAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGA 
                     AGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGA 
                     GGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTAT 
                     ATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACA 
                     TCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGA 
                     CGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAA 
                     GACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGA 
                     TCACTCTCGGCATGGACGAGCTGTACAAG
                     /note="Geneious type: Editing History Deletion"
                     /note="/Original_Bases=ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTG
                     GTGCC 
                     CATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAG 
                     GGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCA 
                     AGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTT 
                     CAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAA 
                     GGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCG 
                     CCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGA 
                     CTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCAC 
                     AACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCC 
                     GCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCC 
                     CATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCC 
                     CTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCG 
                     CCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAG"
     terminator      5927..6180
                     /label=SV40
                     /note="/modified_by=User"
     primer_bind     5943..5965
                     /label=EM820
     misc_feature    6044..6165
                     /label=SV40 polyA signal
     primer_bind     complement(6141..6163)
                     /label=EM1121
     misc_feature    6167..6180
                     /label=EM1745
     protein_bind    6221..6254
                     /label=loxP
     primer_bind     6254..6283
                     /label=Fw2 - PiggyBac NO attP NO lox EM834
     misc_feature    6268..6363
                     /label=repeated transposase region
     misc_feature    complement(6347..6504)
                     /label=original PiggyBac 3'region
     primer_bind     6420..6448
                     /label=EM73
     misc_feature    complement(6442..6460)
                     /label=TR2
     misc_feature    6456..6476
                     /label=direct repeat
     misc_feature    complement(6492..6504)
                     /label=TR1
     primer_bind     complement(6618..6667)
                     /label=EM854
     primer_bind     complement(6634..6650)
                     /label=M13 fwd
     misc_feature    6652..6683
                     /label=BbsI sites GGGG-CCAT
     primer_bind     6668..6703
                     /label=EM841
     misc_feature    join(6682..8825,1..33)
                     /label=pDONR backbone
     terminator      6815..6842
                     /label=rrnB T2 terminator
     primer_bind     complement(7073..7094)
                     /label=EM599
     rep_origin      complement(7172..7760)
                     /direction=LEFT
                     /label=ori
     CDS             complement(7907..8716)
                     /label=KanR
     misc_feature    8787..8805
                     /label=pDONR-RP
ORIGIN
        1 tgccaggaaa cagctatgac catgtaatac gacgatatga tcctgatgca gctagattaa
       61 ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt gctctctctt
      121 tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg cttggagctc
      181 ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata acgaccgcgt
      241 gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact catacgataa
      301 ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata ttttcttgtt
      361 atagattaga tcgcgctcgc gcgactgacg gtcgtaagca cccgcgtacg tgtccacccc
      421 ggtcacaacc ccttgtgtca tgtcggcgac cctacgcccc caactgagag aactcaaagg
      481 ttaccccagt tggggcacta ctcccgaaaa ccgcttctga cctgggaaaa cgtgaagccc
      541 cggggcatcc gctgagggtt gccgccgggg cttcggtgtg tccgtcagta ctgcaggtac
      601 cataacttcg tatagcatac attatacgaa gttataccgg atcctcgcat tttatgagta
      661 aaggcccatt actcatatat gggtaaagtg ctttttgtaa aaaatgagta aatcgattta
      721 ctcataaatt ataatttcac cgtgtttact tttcgtcttt tgttatattt ttgaactgtt
      781 aactattttg tatttatcat ttaattcata caaaattttg cgtttatttt gtagtttatt
      841 gcaatctacg aaaattatta atgaattttt acatgtttca aatactctta atcattttcc
      901 tcctggaaaa cagaccttcc gagacgtagc tctttgaagt tttcataatg atccatgtat
      961 gaaattttcc cctccggctg ttgttcatcg cgcagttgtt cctgttcttc cgttcctcgt
     1021 tcttccggcg cttgaagcct tggtgcagtc ggaactgtgg aaaagactga tatattttta
     1081 tttcacggtc gttaatgaaa atgatgctgt cactatggaa cttacctgca aattccatgc
     1141 ttttgtagcc tttcttgtag ctccacatga tgatttgttg ctcctgtatt atcttcgagt
     1201 accaaacctt gtgaaacgga aataatccat cgtaaatgaa aattgaacgg tatcaattta
     1261 tccaggaagt acttaccgat taaaatatca atgtatttgg gaacctcgac tctaatccgc
     1321 gtgggaatcc gatggacgcg gtatcggtca ttgaacccga tgctgaaggg cctcgactgg
     1381 ctatagcggt gccgtgaaaa tcaaaatatc taaaatttcc cgatagtgtt atgatcaggg
     1441 gatccgcatg cggcaaggta ggcctactgc tcatcttttg cgagtgtgtt agtgtgactg
     1501 gcaacgcgac cagaagtcta gtcgaatcct gcttggaaat cggtttgttt tgtcgatggg
     1561 ggtggcccgc gaaatcgcat tgtgttgttt gggaaggccc gctcgatgca tgttaggtag
     1621 gcgtagtttg ttcgagtttg acgtgcctgc accctccctg gttatgatgg cgcactaatg
     1681 cattccgaga aaagttgcta acttgaaaat tttcatccaa atatgggtaa gttcatttac
     1741 ccatgtttgg gtatagcgat tttacctata atatgggcaa atttgaccgt ttttcaaagg
     1801 tatattttac ccatattatg ggtaatgcat atgaaaccca aatatgggta aatcaactac
     1861 tgatctatgg gtaaactgat cttagcgtgt atgtttcaca cgtgacattc actggtttta
     1921 caattggctt ctttagcagt gttgggataa ttccatatta tgaatctgca tgccaaactg
     1981 ggccgaaatc caaattttca tcaattttgg tgcacgggaa cctatttaaa tatcaatttg
     2041 aagtttgtat gggagcgatt tgtcgaatca cccctcgttg cattttgtac tgggcggagc
     2101 tgtcaaacag ttgcctagct gtcaaaaggt gatttcgaat aatctctttg aaattgattt
     2161 taggtatcaa aataaagttc taaaaatctg aaaaaaatca tagtggctca gaaaaaggtg
     2221 ctctttcgta taaaatcaaa aaatgaacac ttttttcaaa atttaaaaac ccaattttcg
     2281 caaatctata gggctctgca cgaagttctc tccctctctt tcgctctcat tgagattttg
     2341 taaacaacaa ggccaggaaa tgtcaaaatc ccatacaaaa tcaaaacagt gcagtgccct
     2401 atatgtaaaa cacatcactc cgacgtgtaa atttttttga gtgttgattt aatcaaagtg
     2461 aataaaaata ttagttttat gacatacttg ttttctgagt gtagcaaaaa tatgaaaaca
     2521 catttttgta ctttgaatgt taagcgtgta tgctttttgt gtcaatatgt caattgttaa
     2581 acccatgtta atagttttaa ttttttttta atcaaattct ttttttaagt aatgtacagt
     2641 aaaagtgaac ttaatctcat taacaatcaa tttgaataca ttaaaaaata ttgcatatac
     2701 attggaaaca ttcccgccga agtatcgaaa acagatttaa ttgcaaaatt ttccgtattt
     2761 ggtgaaatat ctaacttata catgaagtca ttcattcagt tttgtgatgt gaaaccggca
     2821 gttgttcgtt acagactgat gaaaagtgta aaggaatctt caagtttaca caatagtcga
     2881 tatattcaat cggttttaat agttctgcca ctagattctt cctacaataa ttactttctt
     2941 ccttacaaca cttgtgttgt ggtatacact tataacaaat ttggcatggt agatttttat
     3001 caaaaattca gtaaattagg agatatacat gcgatgaaga aagctacaaa tgtcatggtt
     3061 tacattagct ttgtatcaga aagagctgca aggaccattc tggatactaa gcctacagat
     3121 atacatataa atgtacaaac aattaatcat gttacacgaa atattaacgt atgcttaata
     3181 gattttgaaa aggaatgtac atcaaatacg gcgataaaat taacactttt atataatcgc
     3241 tcaattggaa tattcggact accatctaat ttcacagaag caaaactgca cgatgaattt
     3301 tcaaggtttg tgtggcaatg tgaatccaaa tgagacagat aaatataaaa gctactcaga
     3361 tgaaaagacc ttgaaggtcg aaattagaca ttatattttt atatgttttc aggtatggca
     3421 gaattgaaaa aaatagacta gtgtacgact caaccggaca ctctaaacaa tacggttttg
     3481 tttattatga aaagcacttg tctgctcaag cggccaaaca ggaaatggac cgcagtgatc
     3541 atacaggacg taaaattgca gttcggtttg ttccagaaaa agagtagcat aatcagccat
     3601 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     3661 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     3721 aaataaagca atagcatcac aaatttcaca aataaagcat ttttcttcac tgcattctag
     3781 ttgtggtttg tccaaactca tcaatgtatc taagtatcga atccatcttt acatgtagct
     3841 tgtgcattga atccaattat aatttgcctt ggcaccagct gagccagaca agaaagaaag
     3901 cttcccagaa gtatatcgat ttagaagggt tgacgtcact ttgctgactg cactaataca
     3961 gcaaatgata caattagaat gattcaagtg aaattcccaa attactgctt tgtctctgga
     4021 tttggttatc agattacatt cgaagctaag aatagctacc gaaattgtcg atcaaatcag
     4081 gaaatccttt ctctatcgaa aaaggcattc gcacatcttt ctctgtatgc catatacacg
     4141 aatggtaggt acattgacgt ctttgccaga agttgaactg catcgttcaa ggtacagaat
     4201 gaacgactaa cagacacaag cacgttttgc tgtccattca gacacaggga tggtacccat
     4261 agtcgatcga tttagagcca tccaaccgaa cagaggtata tgtatgaatg gattgcagaa
     4321 attttctaga agtacaacca ccactacggc agtgtctata aaacgcccct gcaaaggcaa
     4381 aaccagctca atcgaatacg tttcctagtg gagtgaacat tacgcggtcc aagtaagcag
     4441 tgccagtgca agtgaagtga agtctctagt gaaaaagagt gatccaatta gccagaggag
     4501 aaaatttcag agtgaacaaa gctttgttca aaggacaatt actattaaat ttgtgaaagt
     4561 gcatttcggt gaagggaatc ttctagtgaa ggtaggtaaa ttaaatgatg aaattatagc
     4621 tatgagcgaa aactagtttg gtgaatgatt cctttgtctt tgaatgagca aactattttc
     4681 caagatggcg actattgagc tttgagtgat tagtgaaaat ttgcaacgca gtttcatcat
     4741 cattgataaa acccaattgt gattcacggc gataatcata tttcgttgaa tcatcgctgc
     4801 taattgaatt aaatttctag agcaagcgcg aattcgccat atttctaaaa ttaaatattg
     4861 tggtgataat tacccattaa ggtaatatta acacatatcg agaaaaacct tgaggaaatc
     4921 gtgaaaactt gaagatacgc aatttccaaa ctacgtagtt caaagtcgaa aacaagttaa
     4981 tttttcacta aaaagtaggg cgttgttgtg acgtcatcac cttcaagtgt atatttttca
     5041 cttggcctgc gactgcaaac gcagacaaag caaaacaagt ttaaaacctg tcgtgtcgtg
     5101 ctcgaagcca aaggcaatga atcaatatca aatgagagtt tgcatttcac aaccaattac
     5161 tgaagcgttt cctcgtttct ttttctgctc aacagagatt tcaacatggt gagcaagggc
     5221 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     5281 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     5341 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     5401 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     5461 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     5521 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     5581 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     5641 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     5701 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     5761 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     5821 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     5881 accgccgccg ggatcactct cggcatggac gagctgtaca agaagcggcc gcgactctag
     5941 atcataatca gccataccac atttgtagag gttacttgct ttaaaaaacc tcccacacct
     6001 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     6061 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6121 actgcattct agttgtggtt tgtccaaact catcaatgta tcttagcttg taattcgtcg
     6181 acgcggatat cgtttaaacc gttgcttgta attcgtcgac ataacttcgt atagcataca
     6241 ttatacgaag ttatgagctc aattcgataa aagttttgtt actttataga agaaattttg
     6301 agtttttgtt ttttttaata aataaataaa cataaataaa ttgtttgttg aatttattat
     6361 tagtatgtaa gtgtaaatat aataaaactt aatatctatt caaattaata aataaacctc
     6421 gatatacaga ccgataaaac acatgcgtca attttacgca tgattatctt taacgtacgt
     6481 cacaatatga ttatctttct agggttaatc tagctgcgtg ttctgcagcg tgtcgagcat
     6541 cttcatctgc tccatcacgc tgtaaaacac atttgcaccg cgagtctgcc cgtcctccac
     6601 gggttcaaaa acgtgaatga acgaggcgcg ctcactggcc gtcgttttac aggggatgtc
     6661 ttcatatata tgaagactcc catctgttgt ttgtcggtga acgctctcct gagtaggaca
     6721 aatccgccgg gagcggattt gaacgttgtg aagcaacggc ccggagggtg gcgggcagga
     6781 cgcccgccat aaactgccag gcatcaaact aagcagaagg ccatcctgac ggatggcctt
     6841 tttgcgtttc tacaaactct tcctggctag cggtacgcgt attaattgcg ttgcgctcac
     6901 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6961 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7021 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7081 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7141 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7201 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7261 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7321 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7381 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7441 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7501 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7561 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7621 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7681 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7741 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7801 ggaacgacgc gtaactcacg ttaagggatt ttggtcatga gcttgcgccg tcccgtcaag
     7861 tcagcgtaat gctctgccag tgttacaacc aattaaccaa ttctgattag aaaaactcat
     7921 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
     7981 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
     8041 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
     8101 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
     8161 atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
     8221 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
     8281 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
     8341 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
     8401 ggaatgctgt ttttccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
     8461 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
     8521 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     8581 cgggcttccc atacaagcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     8641 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc gacgtttccc
     8701 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
     8761 ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga gacacgggcc
     8821 agagc
//
